Labshake search
Citations for Roche :
1 - 50 of 1164 citations for Trans 4 Hydroxy L Proline Rabbit Polyclonal Conjugated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... A polyclonal sheep anti-digoxigenin antibody conjugated to alkaline phosphatase (Roche) was added to membrane at 1:10,000 dilution then washed twice in 1× wash buffer at room temperature with gentle shaking and equilibrated in 1× detection buffer (100 mM Tris-HCl ...
-
bioRxiv - Cancer Biology 2022Quote: ... followed by HRP-conjugated anti-rabbit (Roche) or anti-mouse (Santa Cruz ...
-
bioRxiv - Molecular Biology 2022Quote: ... and L-lactate dehydrogenase from rabbit muscle (Roche) was used as a standard ...
-
bioRxiv - Microbiology 2019Quote: ... 0.5 U/mL L-lactate dehydrogenase from rabbit muscle (Roche) and 1 mM NADH (Roche ...
-
bioRxiv - Cell Biology 2021Quote: ... rabbit polyclonal anti- GFP (gift from Oliver Gruss) and mouse monoclonal anti-GFP (Roche), mouse monoclonal anti-β actin (Sigma) ...
-
bioRxiv - Neuroscience 2020Quote: The following conjugated antibodies were used: sheep polyclonal anti-digoxigenin alkaline phosphatase (1:2000 - 1:5000; Roche Life Science, Cat#11093274910), mouse monoclonal anti-βACTIN (clone AC-15 ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... animals were incubated overnight with peroxidase-conjugated antibodies at 4°C (anti-DIG-POD, Roche 1:500 dilution and anti-DNP ...
-
bioRxiv - Plant Biology 2021Quote: ... After 4 weeks of culture on MSD medium supplemented with 50 mg/L hygromycin (Roche, Germany), the resistant callus lines were transferred onto RM plates to generate transgenic rice seedlings ...
-
bioRxiv - Plant Biology 2020Quote: ... followed by incubation with α-rabbit IgG-HRP-conjugated secondary antibodies (1:10000, Roche, USA) for 1 h at room temperature in a solution of 5 % skim milk in TBS-T ...
-
bioRxiv - Microbiology 2019Quote: ... The GRA7 blot was incubated with a secondary antibody (HRP)-conjugated anti-rabbit antibody (Roche). The HRP was detected using an enhanced chemiluminescence (ECL ...
-
bioRxiv - Developmental Biology 2019Quote: ... the sections were incubated overnight at 4°C with alkaline phosphatase–conjugated antibodies to digoxigenin (Roche) at a dilution of 1:2000 in the same solution ...
-
bioRxiv - Immunology 2022Quote: ... Immunostaining was performed by using 1:500 diluted rabbit polyclonal antibodies against ferroportin (49) and an appropriate detection kit (Omnimap rabbit polyclonal HRP, #760-4311 and ChromoMap-DAB #760-159; Roche). Negative controls were performed by the omission of the primary antibody ...
-
bioRxiv - Neuroscience 2021Quote: ... participants were given either a total of 225mg of L-DOPA (Madopar, Roche, Levodopa/Benserazid, 4:1 ratio) or a placebo (P-Tabletten white 8mm Lichtenstein ...
-
bioRxiv - Developmental Biology 2021Quote: ... They were incubated overnight at 4°C with 1:4000 alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche) in buffer 2 ...
-
bioRxiv - Neuroscience 2020Quote: ... lysates were incubated at 4 °C overnight with rabbit anti-GFP (1:1000, Roche) and pull down was performed with magnetic proteinA beads (Millipore ...
-
bioRxiv - Cell Biology 2023Quote: ... Polyclonal anti-GFP antibody (Roche) was used ...
-
bioRxiv - Neuroscience 2021Quote: ... medium was replaced with E6 medium (DMEM/F-12 supplemented with 64 mg/L L-ascorbic acid 2-phosphate magnesium, 14 µg/L sodium selenium, 543 mg/L sodium bicarbonate, mg/L insulin [Roche, Penzberg ...
-
bioRxiv - Genetics 2022Quote: ... Overnight incubation with Anti-Dig antibody conjugated to alkaline phosphatase (1:5,000) at 4 °C (no. 11093274910; Roche) was followed by 8 × 30 min washing steps at room temperature with TBST 2 (TBST with 0.1% Tween 20 and 0.05% levamisole–tetramisole ...
-
bioRxiv - Developmental Biology 2021Quote: ... and incubated overnight at 4 °C with alkaline phosphatase–conjugated anti-digoxigenin antibodies in TBTX (1:2000; Roche). Following extensive washing in TBTX ...
-
bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation was performed at 4 °C for 1 h with pre-conjugated anti-HA affinity matrix (Roche 11815016001) or Anti-GFP Agarose (RQ2 ...
-
bioRxiv - Cell Biology 2023Quote: ... The polyclonal antibodies anti-GFP (Roche), anti-Arp2 ...
-
bioRxiv - Cell Biology 2020Quote: ... followed by incubation with HRP-conjugated rabbit anti-sheep antibodies and detection using ECL reagents (Roche Diagnostic GmbH). A series of timed exposures were undertaken to ensure that densitometric analyses were performed at exposures within the linear range ...
-
bioRxiv - Microbiology 2024Quote: ... followed by alkaline phosphatase-conjugated anti-rabbit secondary antibodies (1:5000) and CDP-Star (0.25 mM) according to the manufacturer’s instructions (Roche).
-
bioRxiv - Developmental Biology 2020Quote: ... for 1 h and then incubated overnight at 4 °C in block solution with diluted DIG-antibodies (1:5,000) conjugated with alkaline phosphatase (AP) (Roche). To visualize WISH signal ...
-
bioRxiv - Developmental Biology 2021Quote: ... WB following 4-20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) employed HRP-conjugated anti-HA antibody (Roche). Quantification was performed by Image J software.
-
bioRxiv - Neuroscience 2021Quote: ... and incubated overnight at 4°C in B1 buffer with alkaline phosphatase-conjugated anti-DIG (1/2000; Roche 11633716001). After three washes in B1 buffer and one wash in B3 buffer (100 mM Tris–HCl pH 9 ...
-
bioRxiv - Developmental Biology 2020Quote: ... embryos were incubated overnight at 4°C with 1:2000 alkaline phosphatase-conjugated anti-DIG antibodies (Sigma/Roche, 11093274910). After washes and prior to development ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... tissues were incubated at 4°C in a solution of 0.05% alkaline phosphatase-conjugated DIG antibodies (Roche cat #11093274910) overnight ...
-
bioRxiv - Developmental Biology 2021Quote: ... WB following 4-20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) employed HRP-conjugated anti-HA antibody (Roche). Quantification was performed by Image J software.
-
bioRxiv - Immunology 2022Quote: ... Biotinylated human MIF was produced using D-biotinoyl- ε -aminocaproic acid-N-hydroxy-succinimide ester (Biotin-7-NHS) with the Biotin Protein Labeling Kit from Roche (Mannheim, Germany). Alternatively ...
-
Elevated Hoxb5b expands vagal neural crest pool and blocks enteric neuronal development in zebrafishbioRxiv - Developmental Biology 2021Quote: ... Probed embryos were blocked for 1-2 hours at ambient temperature in 5% Goat sera in PBST before detection overnight (∼16 hours) at 4°C using an anti-Digoxigenin-Fab fragments conjugated to Alkaline Phosphatase enzymes (1:1000 dilution, Roche) in 5% Goat Sera in PBST ...
-
bioRxiv - Cell Biology 2021Quote: ... the sections were incubated overnight at 4 °C in digoxigenin antibodies conjugated to horseradish peroxidase (anti-digoxigenin-POD; Fab fragment; 1:100; Roche), rinsed in TBS (0.1 M Tris-HCl with 0.9% NaCl ...
-
bioRxiv - Neuroscience 2019Quote: ... and then incubated overnight at 4°C with sheep anti-DIG alkaline phosphatase conjugated antibody (1:2,000; Roche Applied Sciences). Sections were washed thrice with PTW buffer and twice with coloration buffer (100 mM Tris-HCl ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2% sheep serum in PBST) for one hour and overnight at 4 °C with alkaline phosphatase-conjugated anti-DIG antibodies (Roche) diluted at 1:4000 in the blocking solution ...
-
bioRxiv - Microbiology 2022Quote: ... Hybridised probes were detected with anti-DIG antibodies and revealed with alkaline phosphatase-conjugated antibodies in presence of nitro blue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP, Roche).
-
bioRxiv - Cell Biology 2022Quote: ... The membrane was hybridized with a TAA(CCCTAA)4 probe which was conjugated with digoxigenin (DIG oligonucleotide 3⍰-end labelling kit, Roche) and signal was revealed using the anti-DIG-alkaline phosphatase antibodies (Roche ...
-
bioRxiv - Developmental Biology 2023Quote: ... 2% sheep serum in PBST) for one hour and overnight at 4 °C with alkaline phosphatase-conjugated anti-DIG antibodies (Roche) diluted at 1/4000 in the blocking solution ...
-
bioRxiv - Neuroscience 2024Quote: ... the sections were washed and incubated overnight at 4 °C with an alkaline phosphatase-conjugated anti-digoxigenin antibody (1:2500-1:4000, Roche). To visualize the RNA-probe binding ...
-
bioRxiv - Cell Biology 2021Quote: ... and mouse/rabbit IgG overnight at 4°C and further with protein G-coupled agarose beads (ROCHE) for 1-2 h ...
-
bioRxiv - Developmental Biology 2023Quote: ... Embryos were then incubated overnight at 4°C with a 1:2000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche #11093274910) in MABT blocking buffer with 1% sheep serum ...
-
bioRxiv - Developmental Biology 2023Quote: ... Embryos were then incubated overnight at 4°C with a 1:2000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche #11093274910) in MABT blocking buffer with 1% sheep serum ...
-
bioRxiv - Genomics 2023Quote: ... sheep polyclonal anti-DIG-FITC (1:500, Roche). As the secondary antibodies we used Alexa Fluor 633-conjugated goat anti-guinea pig IgG (Invitrogen) ...
-
bioRxiv - Physiology 2022Quote: ... 2 mmol/l MgCl2, 10 mmol/l NaF, 1 mmol/l PMSF, 1% Triton X-100 and Complete Protease inhibitor mixture, Roche Diagnostics) for 30 min on ice and centrifuged at 20,000 g for 10 min at 4ºC ...
-
bioRxiv - Neuroscience 2019Quote: ... The sections were then stained overnight at 4°C with pre-absorbed AP-conjugated sheep anti-DIG antibody (1:1000, Roche, cat. 11093274910) in PBS containing 0.1% Triton X-100 and 20% horse serum ...
-
bioRxiv - Neuroscience 2020Quote: ... and blocked for 1 h (at room temperature) before incubating overnight at 4 °C in anti-dig sheep Fab fragments conjugated to alkaline phosphatase (AP; Roche No. 11093274910) diluted 1:2500 in blocking solution ...
-
bioRxiv - Molecular Biology 2020Quote: ... Signals were detected using anti-rabbit IgG conjugated with alkaline phosphatase and the color reaction substrates NBT-BCIP (Roche Biochemicals, Germany).
-
bioRxiv - Genomics 2021Quote: ... Samples were then incubated overnight at 4°C with a rabbit anti-DIG HRP-conjugate antibody (1:500, Roche). Then ...
-
bioRxiv - Immunology 2023Quote: ... Primary antibodies were incubated overnight at 4°C: rabbit anti-HA high affinity at 1:500 (Roche Diagnostics #ROAHAHA), mouse anti-FLAG M2 at 1:500 (Sigma-Aldrich #F1804) ...
-
bioRxiv - Neuroscience 2021Quote: ... and anti-GFP (mouse polyclonal, Roche, 11867423001, 1:5000). After washing three times with TBST buffer ...
-
bioRxiv - Biophysics 2021Quote: ... 5 mg of sheep polyclonal anti-digoxigenin antibody (Roche) in 100 ml with 10 mM PBS were incubated in the capillary for 2 h at 37°C ...