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Citations for Roche :
1 - 50 of 354 citations for Siglec 15 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK293 cells were transiently transfected with β-arrestin 2-Renilla luciferase 8 (Rluc8) and human C5aR2-Venus constructs using XTG9 (Roche, Sydney, Australia) for 24 h ...
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bioRxiv - Cancer Biology 2023Quote: ... the T cells were activated in X-VIVO 15 containing 150 U/mL of human IL-2 (#Ro 23-6019, Roche, Switzerland), 10 ng/mL of recombinant IL-7 (#200-07 ...
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bioRxiv - Immunology 2020Quote: ... 1% FCS and 12.5µg/ml DNAse (Roche) in IMDM (Gibco) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The plasmid constructs were transfected into HEK293 cells by using X-tremeGENE HP (Roche), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cells were transiently transfected with C5aR2-Venus and βarr2-Rluc8 constructs using XTG9 (Roche). 24 hours post transfection ...
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bioRxiv - Developmental Biology 2020Quote: ... 15 µg/ml transferrin (Roche), 450 µM monothioglycerol (Sigma) ...
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bioRxiv - Genomics 2022Quote: ... 15 U/μL TdT (Roche), 0.1 U/μL RNase H (QIAGEN ...
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bioRxiv - Immunology 2024Quote: ... Zfp366_rev#15: TCT GGG AGA GGT CAA ATG GT and universal probe library # 15 (Roche); Actb ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 2% FCS and 1mg/mL dispase II (Roche) overnight at 4°C ...
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bioRxiv - Genetics 2019Quote: Total protein was extracted from transfected HEK293 cells lysed in RIPA buffer (Pierce) containing protease inhibitors (Roche) 24 hours post transfection and size separated using SDS-PAGE ...
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bioRxiv - Genetics 2019Quote: ... 15 units of FastStart Taq (Roche). Reactions were heated 95°C for 5 min ...
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bioRxiv - Biophysics 2021Quote: ... 15 μg/mL DNase I (Roche), 0.5 mg/mL Lysozyme ...
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bioRxiv - Bioengineering 2019Quote: ... Human Fibronectin (Roche). Agar low melting point ...
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bioRxiv - Developmental Biology 2022Quote: ... An MNase enzyme (15 units; Roche, #10107921001) was added to aliquots containing 2 million spermatozoa in an MNase buffer (0.3 M sucrose ...
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bioRxiv - Cell Biology 2019Quote: ... 15 μg/ml insulin (Cat.#1376497, Roche), 7 μg/ml transferrin (Cat.# 652202 ...
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bioRxiv - Neuroscience 2023Quote: ... and 15 mg/ml transferrin (Roche, #10652202001). Once cells reached the neural epithelial stage at day 5 ...
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bioRxiv - Cell Biology 2021Quote: ... human plasma fibronectin (Roche) was conjugated with Atto-647N using a protein labeling kit (cat # 76508 Sigma-Aldrich) ...
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bioRxiv - Genomics 2023Quote: ... Human genomic DNA (Roche) was amplified ...
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bioRxiv - Physiology 2020Quote: ... Patch-clamp experiments on HCN1 and HCN2 were performed with transient transfection of 2 μg of either pCDNA3.1-HCN1 or pCDNA3.1-HCN2 (generously provided by Dr. Eric Accili) in HEK293 cells using Fugene6 (Roche, Basal, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... were transfected into HEK293 cells at a ratio of 3:3:1 (hGluN1/hGluN2A/EGFP) using X-tremegene HP (Roche) at a 1:100 ratio with optiMEM ...
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bioRxiv - Biochemistry 2024Quote: ... The pcDNA3 constructs were used for transient transfection in HEK293 cells using an X-tremeGENE HP DNA Transfection Reagent (Roche, 6366236001according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and incubated with intermittent shaking (30 s shaking, 30 s off) for 15 min with 15 units of DNAse I (Roche) to remove DNA traces ...
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bioRxiv - Microbiology 2020Quote: ... 15% glycerol and cOmplete Protease Inhibitor Cocktail (Roche)) with 50 µl of acid-washed glass beads (Sigma)7 ...
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bioRxiv - Neuroscience 2021Quote: ... 15 mM β-glycerophosphate and protease inhibitors (Roche), pH 7.4 ...
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bioRxiv - Developmental Biology 2022Quote: ... and 15 µg/ml of transferrin (Roche, #10652202001). 24 hours after seeding in the 24-well plate ...
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bioRxiv - Molecular Biology 2024Quote: ... with oligo-p(dt)15 (Roche®, 10814270001). The cDNAs were analyzed by real time PCR using SYBR Green (Bimake ...
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bioRxiv - Immunology 2024Quote: ... 1X EHF buffer with 15 mM MgCl2 (Roche), 1 U HiFi enzyme mix (Expand High Fidelity PCR System ...
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bioRxiv - Microbiology 2021Quote: ... anti-human CD4 clone SP35 and anti-human CD8 clone SP57 (Roche, Basel, Switzerland), and anti-human CD20 clone L26 Dako Omnis (Agilent ...
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bioRxiv - Molecular Biology 2020Quote: ... 5% CO2 for 24 h before transfection with a 4:1 DNA ratio of pCMV6-Affimer-tGFP and FLAG-ERK plasmids using Lipofectamine 2000 (SW620, HEK293, NCI-H460) or X-tremeGENE 9 (Roche; Panc10.05). After 24 h cells were serum starved for 1 h ...
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bioRxiv - Genetics 2020Quote: N-terminal HA-tagged PRUNE1 overexpression constructs were transfected into PRUNE1 non-expressing HEK293 cells using X-tremeGENE HP DNA (Roche; 06366236001) transfection reagent ...
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bioRxiv - Biochemistry 2019Quote: ... The transfection of GFP-RNase H1 R-loop-binding domain (GFP-HB) for R-loop detection into HEK293 cells was performed with the FuGENE Transfection reagent (Roche, E269A).
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bioRxiv - Cancer Biology 2023Quote: ... This plasmid was digested with PacI and transfected into HEK293 cells using X-tremeGENETM HP DNA transfection reagent (Roche, Basel, Switzerland) to produce infectious viral particles ...
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bioRxiv - Biophysics 2020Quote: ... fibronectin from human plasma (Roche), the final concentration used for the experiments was 50 μg/ml (containing 2/3 of bovine and 1/3 of human FN) ...
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bioRxiv - Developmental Biology 2019Quote: ... human holotransferrin 0.6% (Roche, 10652202001); monothioglycerol 0.0039 % (Sigma ...
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bioRxiv - Immunology 2019Quote: ... human recombinant IL-2 (Roche) was also added fresh immediately prior to use at a final concentration of 100 IU/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Human fibronectin (Roche Diagnostics, 11051407001); Puromycin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... and human insulin (11376497, Roche) were digested with recombinant WT or protease-dead (cf-E111Q ...
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bioRxiv - Molecular Biology 2020Quote: ... 15 mM MgOAc, 15 mM Tris-HCl pH 7.4, 0.5% NP40, 1 mM DTT, 1 μl RNAsin, Roche protease inhibitors without EDTA). The cells were lysed by one freeze-thaw cycle in large volume (15–50 ml ...
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bioRxiv - Biochemistry 2020Quote: ... MR-Fc was purified from cell culture supernatant using Protein A-agarose beads (Roche). For ELISA experiments ...
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bioRxiv - Immunology 2019Quote: ... RNA was reverse transcribed with pd(T)-15 (Roche) and Moloney Murine Leukemia Virus reverse transcriptase (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... 15 mM NaCl and protease and phosphatase inhibitors (Roche) for protein extraction ...
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bioRxiv - Physiology 2019Quote: ... BDM 15 and 200UI/mL collagenase A (Roche Diagnostics) plus bovine serum albumin 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... BDM 15 and 200UI/mL collagenase A (Roche Diagnostics) plus 0.5% bovine serum albumin ...
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bioRxiv - Genetics 2023Quote: ... 15 ul of 2X FastStart™ PCR Master (Roche), 2 ul of DMSO ...
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bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Roche Cat#11691112001) was purchased from Sigma (Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Roche Cat#11691112001) was purchased from Sigma (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... 180 g/ml human transferrin (Roche), 5 ng/ml VEGF (PeproTech 450-32) ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IFNγ from Roche (#11040596001), recombinant human IL-1β from PeproTech (#200-01B).
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bioRxiv - Cancer Biology 2020Quote: ... 180 μg/ml human transferrin (Roche). Flk-1+ cells were isolated by magnetic cell sorting (MACS ...
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bioRxiv - Cancer Biology 2020Quote: ... 15 μl of 20 mg/ml RNase A (Roche 10109169001) was added to the samples that were then quickly vortexed and incubated at 37°C for 30 minutes ...