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Citations for Roche :
1 - 50 of 233 citations for SARS Coronavirus Nucleoprotein N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: We then targeted the N-(nucleoprotein) gene of SARS-CoV-2 virus with Roche Lightcycler 480 RNA Master Hydrolysis Probes (Roche Basel, Switzerland) with primers developed in house ...
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bioRxiv - Genomics 2019Quote: ... we used Escherichia coli k-12 strain MG1655 (hereafter E. coli; Roche, Basel, Switzerland), which has a high-quality genome sequence available (GenBank accession NC_000913 ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR analysis was conducted using anti-SARS-CoV-2 primer pairs specific to the E-gene (Corman et al. 2020) and Lightmix Modular SARS and Wuhan CoV E-gene (Roche Lifescience, Basel, Switzerland) in an ABI 7500 Fast PCR cycler (PE applied biosystems ...
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bioRxiv - Microbiology 2021Quote: ... and LacZ (β-D-galactosidase of E. coli (Hoffmann-La Roche, Basel, Switzerland) were used ...
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bioRxiv - Immunology 2024Quote: ... Sera were analysed for antibody directed against the SARS-CoV-2 Spike protein receptor-binding domain (S-RBD) and nucleocapsid (N) using an established automated electrochemiluminescence assay (Elecsys Anti-SARS-CoV-2 S and N, Roche diagnostics) [54] ...
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bioRxiv - Microbiology 2024Quote: ... Asymptomatic or undetected infections of the vaccinated-only group were excluded in those samples by testing all specimens for antibodies against SARS-CoV-2 nucleocapsid (Roche Elecsys anti-SARS-CoV-2 N assay), and only specimens with negative result were used ...
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bioRxiv - Microbiology 2024Quote: ... BHK cells were transfected with 3 μg of pBAC-SARS-COV-2 construct and1 μg of SARS-CoV-2 N (Delta) plasmid with the X-treme Gene 9 (X9) DNA transfection reagent (Roche: 6365809001) was added 1:3 as DNA:X9 ...
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bioRxiv - Immunology 2022Quote: Anti-SARS-CoV-2 (N protein) IgM/IgG levels in the serum were measured using an Elecsys Anti-SARS-CoV-2 with cobas8000 (Roche Diagnostics KK) at the Department of Clinical Laboratory ...
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bioRxiv - Immunology 2020Quote: ... The treated samples were purified using a C18 cartridge (Oasis HLB Plus Waters) prior to the release of N-glycans by PNGase F (recombinant from Escherichia coli, Roche) digestion ...
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bioRxiv - Immunology 2021Quote: We used DOTAP (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate) (Roche) to introduce LPS or oxPLs into cells following the method by Zanoni et al (Zanoni et al. ...
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bioRxiv - Microbiology 2022Quote: ... 25 uL of N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methylsulfate (DOTAP) (Roche Diagnostics Deutschland GmbH ...
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bioRxiv - Cell Biology 2022Quote: ... coli tRNA (Roche), 1 U/μl RNasin Plus (Promega)) ...
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bioRxiv - Neuroscience 2022Quote: ... coli tRNA (Roche), 2 mM Ribonucleoside Vanadyl Complexes (RVC ...
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bioRxiv - Molecular Biology 2022Quote: ... coli tRNA (Roche) was added ...
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bioRxiv - Immunology 2021Quote: ... with Elecsys Anti-SARS-CoV-2 (Roche), and <1 was evaluated as seronegative according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1 mM ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA) and protease inhibitor cocktail (Roche) on ice for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... Two µg plasmid DNA and 25 µL DOTAP (N-[1-(2,3- Dioleoyloxy)propyl]-N,N,N-trimethylammonium methylsulfate) (Roche Diagnostics Deutschland GmbH ...
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bioRxiv - Bioengineering 2020Quote: ... coli tRNA mixture (Roche), 4 mM oxidized glutathione (GSSG) ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli tRNA (Roche 10108294001), 2 mM each of 20 amino acids ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli tRNA (Roche 10108294001), 2 mM each of 20 amino acids ...
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bioRxiv - Molecular Biology 2019Quote: ... coli MRE 600 (Roche). Picked cells suspended in the lysis were stored at −80°C until further processing ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli tRNA (Roche 10108294001), 2 mM each of 20 amino acids ...
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bioRxiv - Biochemistry 2022Quote: ... coli MRE 600 (Roche), 227.5 μg/μL Folinic acid pH 7.2) ...
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bioRxiv - Microbiology 2023Quote: ... coli tRNA (Roche, #10109541001), 1mM vanadyl ribonucleoside complex (VRC ...
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bioRxiv - Molecular Biology 2023Quote: ... coli MRE 600 (Roche) in 1 ml of hybridization buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... coli tRNA (Roche, 10109541001)] and Mix2 [0.4 mg/mL BSA (NEB ...
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bioRxiv - Microbiology 2024Quote: ... coli tRNA mix (Roche), 14 mM DTT ...
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bioRxiv - Biophysics 2024Quote: ... coli MRE 600 (Roche), 227.5 μg/μL Folinic acid ...
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bioRxiv - Immunology 2023Quote: ... Cationic lipid N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate (Dotap, Liposomal Transfection Reagent, Cat#1202375, Roche, Nonnenwald, Penzberg, Germany) was used for the conjugation of phosphorothioated miRNAs and Dotap-formulated miRNAs were used for in vitro delivery of miRNAs ...
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bioRxiv - Immunology 2023Quote: ... Elecsys® Anti-SARS-CoV-2 S from Roche for the in vitro quantitative determination of antibodies (including IgG ...
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bioRxiv - Physiology 2019Quote: ... The Cobas e 601 (Roche, Germany) electrochemiluminescence device was used to determine the concentrations of serum FSH ...
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bioRxiv - Molecular Biology 2021Quote: ... coli tRNA was from Roche, and oligonucleotides were from Microsynth ...
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bioRxiv - Cell Biology 2021Quote: ... coli tRNA (Roche, TRNAMRE-RO). Note VRC and probe competitor were omitted for half the cells analyzed in replicate 1 ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21(DE3) pLysS (Roche) cells and co-expression with GroEL was performed at 16°C for 18 hours in 500 ml of Luria–Bertani medium containing 100 μg/l ampicillin and 25 μg/l chloramphenicol ...
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bioRxiv - Immunology 2021Quote: ... N-Glycans were released by 10 U N-glycosidase F (Roche) digestion in 50 mM NH4HCO3 buffer pH 8.4 ...
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bioRxiv - Cancer Biology 2023Quote: ... For N-glycan release 2.5 U N-glycosidase F (Roche, DE) were added and the resulting mixture was incubated overnight at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... HUVEC were seeded to confluency on the microelectrodes of the E-plate (E-plate 16, Roche Applied Science). Electrical impedance readings were taken every 2 min for 5h ...
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bioRxiv - Immunology 2021Quote: ... and 0.5% Zwittergent 3-12 (N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate) (pH 8.0) and the presence of Complete® protease inhibitor (Roche). The preparation was centrifuged for 10 min at 2500 rpm and 4 °C and supernatant was transferred to a new tube and centrifuged for 40 min at 30,000 x g and 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% Zwittergent 3-12 (N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate) and protease inhibitor (Complete, Roche Applied Science)) for 2 h at 0°C (ref A) ...
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bioRxiv - Genomics 2022Quote: ... Positivity of SARS-CoV-2 infection was assessed both by PCR and measurement of specific antibodies (Cobas-Roche using Elecsys Anti-SARS-CoV-2 S). All patients gave their consent for participation in this study ...
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bioRxiv - Immunology 2023Quote: ... only donors were included that had no history of COVID-19 as well as had tested negative in three serological assays (EuroImmun-Anti-SARS-CoV-2 ELISA IgG /S1/, Siemens Healthineers SARS-CoV-2 IgG /RBD/, and Roche Elecsys Ig /Nucleocapsid Pan Ig/). PBMC were selected from heparinized full blood by a standard density gradient (Pancoll Separating Solution ...
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bioRxiv - Developmental Biology 2023Quote: ... HUVEC were seeded at a density of 60,000cells/well of the E-plate (E-plate 16, Roche Applied Science), then electrical impedance readings acquired every 2 min for 24 hr ...
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bioRxiv - Immunology 2020Quote: ... or the Roche Cobas SARS-CoV-2 Test CE-IVD (Roche) according to manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 6.7 mM N,N-dimethylcyclohexylamine (DMCH) and EDTA-free complete protease inhibitor tablets (Roche). The mixture was incubated at 4°C with rotation for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... coli 23S rRNA spike-in (Roche, 10206938001) using RT-qPCR (20 picograms of E ...
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bioRxiv - Microbiology 2020Quote: ... coli cultures were supplemented with Kanamycin (Roche) at a final concentration of 50µg/ml ...
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bioRxiv - Microbiology 2024Quote: ... N-Glycosidase F (Roche, #11365185001) was diluted 1:10 in serum-free IMDM and incubated with the cells for two hours at 37°C prior to fixation ...
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bioRxiv - Cell Biology 2023Quote: ... N-Glycans were enzymatically released by peptide N-glycosidase F (E.C. 3.5.1.52; Roche Applied Science) digestion then purified by C18-Sep-Pak (Waters Corp. ...
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bioRxiv - Cell Biology 2020Quote: ... HUVEC were pre-treated with siRNAs or drug for 24 hr prior to plating an equal cell number onto the microelectrode surface of the E-plate (E-plate 16, Roche Applied Science). Impedance readings were taken automatically every 5 min for 24 hr.
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bioRxiv - Synthetic Biology 2022Quote: ... coli tRNA and Phosphoenolpyruvate were purchased from Roche. Bpy and AzK were purchased from Toronto Research Chemicals ...