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Citations for Roche :
1 - 50 of 358 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... His-tagged proteins in soluble fraction were purified using cOmplete His-Tag Purification Columns (Roche). The columns were washed with 10 column volumes of wash buffer 1 (20 mM Tris ...
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bioRxiv - Genomics 2019Quote: ... we used Escherichia coli k-12 strain MG1655 (hereafter E. coli; Roche, Basel, Switzerland), which has a high-quality genome sequence available (GenBank accession NC_000913 ...
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bioRxiv - Biophysics 2022Quote: ... the supernatants containing the His-tagged histones were combined with NiNTA resin (Complete His-Tag purification Resin, Roche) (1mL of Ni-NTA per 1L of bacteria ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged CopS(34-151) was purified using Ni-NTA columns (Roche)(11) ...
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bioRxiv - Biophysics 2022Quote: ... His-tagged ecMSG was loaded onto a gravity nickel affinity column (Roche) and eluted using 300 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... lysate supernatant from cells expressing N-terminal 6-His-tagged proteins were incubated with nickel-nitrilotriacetic acid (Ni-NTA) cOmplete His-tag purification resin (Roche) for 1 h at 4° C ...
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bioRxiv - Genetics 2022Quote: ... MYC-tagged or FLAG-tagged recombinant proteins were captured using ~5μg antibody and Protein-Agarose beads (Roche Applied Science, 11243233001) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The recombinant biotinylated A33-His antigen was immobilized on streptavidin (StreptaWells, Roche) or avidin coated wells (Avidin ...
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bioRxiv - Microbiology 2021Quote: ... and LacZ (β-D-galactosidase of E. coli (Hoffmann-La Roche, Basel, Switzerland) were used ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant cloned from Flavobacterium meningosepticum and expressed in Escherichia coli) was purchased from Roche Diagnostics (Mannheim ...
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bioRxiv - Plant Biology 2021Quote: ... His-tagged AtTPL188 (wt and mutants) bacteria pellets were resuspended in buffer A with EDTA-free antiprotease (Roche). The soluble fractions recovered after sonication were passed through a Ni-sepharose (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... The His-tagged-TRF2TRFH protein was purified in presence of cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche) by using Protino ® Ni-TED 2000 Packed Columns (Macherey-Nagel ...
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bioRxiv - Bioengineering 2024Quote: ... His-tagged rhFGFb was detected using a primary anti-HISx6 mouse polyclonal antibody (Roche Molecular Systems, Inc., USA) or anti-FGF polyclonal antibody (Santa Cruz Biotechnology ...
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bioRxiv - Bioengineering 2023Quote: ... coli DH10B recombinant strain was extracted using the High Pure Plasmid Purification Kit (Roche, Basel, Switzerland), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... HA-epitope tagged recombinant proteins were detected using a rat-derived monoclonal anti-HA antibody (dilution 1:200, Roche) followed by a secondary anti-rat antibody coupled to AlexaFluor 594 fluorophores (dilution 1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... HA-epitope tagged recombinant proteins were detected using a rat-derived monoclonal anti-HA antibody (dilution 1:200, Roche) followed by a secondary anti-rat antibody coupled to AlexaFluor 488 fluorophores (dilution 1:200 ...
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Serine-ubiquitination regulates Golgi morphology and the secretory pathway upon Legionella infectionbioRxiv - Cell Biology 2020Quote: ... His (Roche), GRASP55 (Proteintech) ...
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bioRxiv - Immunology 2020Quote: ... The treated samples were purified using a C18 cartridge (Oasis HLB Plus Waters) prior to the release of N-glycans by PNGase F (recombinant from Escherichia coli, Roche) digestion ...
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bioRxiv - Molecular Biology 2019Quote: ... hybridized fluorescein tagged dUTP was detected with alkaline phosphatase tagged anti fluorescein Fab fragments (Roche), revealed with CDP-Star (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... coli tRNA (Roche), 1 U/μl RNasin Plus (Promega)) ...
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bioRxiv - Neuroscience 2022Quote: ... coli tRNA (Roche), 2 mM Ribonucleoside Vanadyl Complexes (RVC ...
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bioRxiv - Molecular Biology 2022Quote: ... coli tRNA (Roche) was added ...
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bioRxiv - Cell Biology 2022Quote: ... and His-tag (11965085001, Roche) antibodies were used to detect the proteins.
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bioRxiv - Biochemistry 2019Quote: ... anti-His-tag (11922416001; Roche), anti-vinculin (V4505 ...
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bioRxiv - Bioengineering 2020Quote: ... coli tRNA mixture (Roche), 4 mM oxidized glutathione (GSSG) ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli tRNA (Roche 10108294001), 2 mM each of 20 amino acids ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli tRNA (Roche 10108294001), 2 mM each of 20 amino acids ...
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bioRxiv - Molecular Biology 2019Quote: ... coli MRE 600 (Roche). Picked cells suspended in the lysis were stored at −80°C until further processing ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli tRNA (Roche 10108294001), 2 mM each of 20 amino acids ...
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bioRxiv - Biochemistry 2022Quote: ... coli MRE 600 (Roche), 227.5 μg/μL Folinic acid pH 7.2) ...
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bioRxiv - Microbiology 2023Quote: ... coli tRNA (Roche, #10109541001), 1mM vanadyl ribonucleoside complex (VRC ...
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bioRxiv - Molecular Biology 2023Quote: ... coli MRE 600 (Roche) in 1 ml of hybridization buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... coli tRNA (Roche, 10109541001)] and Mix2 [0.4 mg/mL BSA (NEB ...
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bioRxiv - Microbiology 2024Quote: ... coli tRNA mix (Roche), 14 mM DTT ...
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bioRxiv - Biophysics 2024Quote: ... coli MRE 600 (Roche), 227.5 μg/μL Folinic acid ...
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bioRxiv - Biochemistry 2021Quote: ... cOmplete His-Tag Purification Resin (Roche) was added to the supernatant and samples were incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Either His (Thermo) or GFP (Roche) or GST (Santa Cruz ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoblot with the anti-His-antibody conjugated to peroxidase (BMG-His-1 monoclonal antibody; Roche, Basel, Switzerland) was used to confirm the presence of enzymes.
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bioRxiv - Physiology 2019Quote: ... The Cobas e 601 (Roche, Germany) electrochemiluminescence device was used to determine the concentrations of serum FSH ...
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bioRxiv - Biochemistry 2022Quote: (His)6-GST-SNX15 MIT was bound to cOmplete His-Tag purification beads (5 mL, Roche, Germany, 2h) and washed with 2 L wash buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... coli tRNA was from Roche, and oligonucleotides were from Microsynth ...
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bioRxiv - Cell Biology 2021Quote: ... coli tRNA (Roche, TRNAMRE-RO). Note VRC and probe competitor were omitted for half the cells analyzed in replicate 1 ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21(DE3) pLysS (Roche) cells and co-expression with GroEL was performed at 16°C for 18 hours in 500 ml of Luria–Bertani medium containing 100 μg/l ampicillin and 25 μg/l chloramphenicol ...
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bioRxiv - Plant Biology 2024Quote: ... and 1/5000 anti-His (11667475001, Roche) for GFP and His-Tag detection ...
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bioRxiv - Immunology 2019Quote: ... human recombinant IL-2 (Roche) was also added fresh immediately prior to use at a final concentration of 100 IU/ml ...
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bioRxiv - Molecular Biology 2022Quote: Recombinant HBsAg (Roche Diagnostics International) was used as a marker for aerosol formation ...
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bioRxiv - Synthetic Biology 2022Quote: ... with recombinant DNase I (Roche) treatment ...
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bioRxiv - Synthetic Biology 2022Quote: ... and recombinant proteinase K (Roche). Samples were run on a 1% certified megabase agarose (Bio-Rad ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 8G8-Fab were expressed with His-tag and purified using the cOmplete His-Tag purification resin (Roche, USA) following standard protocols ...
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bioRxiv - Developmental Biology 2024Quote: ... ST-HSCs and LT-HSCs using a KAPA RNA HyperPrep kit with RiboErase (HMR) (Kapa Biosystems) and sequenced using a Hiseq 1500 (Illumina ...