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Citations for Roche :
1 - 50 of 6039 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... 6μL PCR mix (1x KAPA HiFi PCR buffer(Roche), 0.3mM dNTPs/each(Roche) ...
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bioRxiv - Genetics 2019Quote: ... 1× FastStart PCR Reaction buffer (Roche) (50mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: Single cells were sorted directly into PCR tubes containing 25 μl PCR lysis buffer (1x Buffer 1 from the Expand Long Template PCR System [Roche], 0.5 mg/ml Proteinase K) and lysed for 1h at 55°C ...
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bioRxiv - Cell Biology 2020Quote: ... or 1x Kappa HiFi PCR buffer (Kapa Biosystems). For locally doublestranded cleavage tests pBB3 was added to a final concentration of 3 μM ...
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bioRxiv - Cell Biology 2024Quote: ... 1× KAPA Hotstart HiFI PCR buffer (Roche, #THSNTKB), 0.02 U µl−1 of KAPA HiFi Hotstart polymerase (Roche ...
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bioRxiv - Cancer Biology 2019Quote: ... 1x FastStart PCR Buffer including 2 μM MgCl2 (Roche), 0.05 mm dNTPs (Roche) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 25μl of 1X PCR buffer B (Kapa Biosystems, Boston, MA, USA) pre-warmed at 65°C was added then the legs were ground ...
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bioRxiv - Genomics 2021Quote: ... all containing constant reaction concentrations of (1x KAPA HiFi PCR buffer(Roche), 0.3mM dNTPs/each(Roche) ...
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bioRxiv - Systems Biology 2022Quote: ... PCR was immediately performed by adding 6ul of PCR mix to each well (2ul Kapa HiFi Hotstart buffer (5x, Roche), dNTPs 0.12ul (25mM/each ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR was performed using KAPA-Hifi polymerase with 1X KAPA HiFi Buffer (Roche), 0.2 mM dNTPs and 1.0 µm of each forward (5’-CCGGTTGTACCTATCGAGTG-3’ ...
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bioRxiv - Molecular Biology 2019Quote: ... Each PCR reaction was composed of the following ingredients: 1 μl 10× FastStart PCR Buffer comprising 20 mM MgCl2 (Roche Diagnostics), 0.2 μl dNTPs (10 mM each) ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted using a Tissue Lysis Buffer and PCR template preparation kit (Roche).
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bioRxiv - Immunology 2021Quote: ... 5 μl of the resulting cDNA-containing reverse transcription mixes were then used as templates for 25 μL PCR reactions using the KAPA HiFi PCR kit with GC buffer (Roche Diagnostics, #07958846001) and the following thermal cycling conditions ...
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bioRxiv - Plant Biology 2019Quote: ... 2 μL of 10x PCR Reaction Buffer with MgCl2 (Roche Life Science, Indianapolis, IN, USA), 0.15 μL Taq DNA Polymerase (Roche Life Science ...
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bioRxiv - Molecular Biology 2021Quote: ... The following PCR conditions were used: 5µL KAPA HiFi Hotstart Fidelity Buffer (5X) (KAPA Biosystems, USA), 1.25µL Forward Primer (10µM) ...
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bioRxiv - Microbiology 2020Quote: ... Amplicon PCRs were conducted in 25 μL reactions each containing 1 X Buffer (KAPA Biosystems, USA), 1.5 mM MgCl2 ...
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bioRxiv - Microbiology 2021Quote: ... PCRs were prepared containing 1x KAPA Taq Buffer A (includes 1.5 mM MgCl2) (Kapa Biosystems, USA), dNTP nucleotide mix (0.2 mM each) ...
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bioRxiv - Microbiology 2023Quote: ... The ERIC-PCR was performed with 25-μL volumes containing Taq DNA polymerase buffer 1X (Roche), 200 mM dNTP (Sigma-Aldrich) ...
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bioRxiv - Genomics 2021Quote: ... Then 100 nL of KAPA PCR mix (2.775 X HiFi Buffer, 0.85 mM dNTPs, 0.05 U KAPA HiFi polymerase / µL)(Roche Cat# 07958846001) was added to all wells containing a single nucleus via two 50 nL filtered dispenses ...
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bioRxiv - Immunology 2021Quote: Single-cells were sorted into PCR tubes containing 1μl of cell lysis solution (1:10 Cell Lysis buffer(Roche), 10U/μl Rnasin plus Ribonuclease inhibitor (Promega ...
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bioRxiv - Physiology 2019Quote: ... The qRT-PCR reactions were carried out in triplicate in 96-well plates and each PCR sample consisted of 6μl 2X SYBR green master mix buffer (Roche), 0.024μl of forward and reverse primers 25nmole and 3.953μl of RNase-free water ...
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bioRxiv - Genomics 2021Quote: ... KAPA PCR mix (2.775 X HiFi Buffer, 0.85 mM dNTPs, 0.05 U KAPA HiFi polymerase / µL, Roche Cat# 07958846001). The chip was sealed for heated incubation and spun down at 1200 x g for 1 min after each dispense ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... resuspended at 1 million cells per mL in CETSA buffer and dispensed (10 μL per well) into 384-well PCR plates (Roche) using a Multidrop Combi (ThermoFisher) ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was immobilized using UV crosslinking (125 mJ/cm2) and hybridized at 42°C using Abion ULTRAhyb hybridization buffer and PCR DIG probes (Roche) synthesised according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2020Quote: ... A 25 μl PCR master mix was prepared in a UV-sterilized hood and consisted of 3.125 μl Buffer (Kapa Biosystems), 1.25 μl dNTP (Invitrogen) ...
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bioRxiv - Biophysics 2020Quote: ... Beads in the sample and control fractions were then pulled down and the looping buffer was replaced with 50 μl of PCR mix (25 μl 2x HiFi KAPA Hot Start ready mix (Roche), 1 ul each of 100 μM primers (supplementary note 1) ...
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bioRxiv - Systems Biology 2024Quote: ... 15uL of the first round products were mixed into a second PCR reaction that contained 1x KAPA HiFi GC Buffer (Roche), 300uM dNTPs ...
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bioRxiv - Molecular Biology 2019Quote: Re-amplification of primary WTA products was performed in a reaction volume of 50 μl comprising 5 μl Expand Long Template Buffer 1 (Expand Long Template PCR System, Roche Diagnostics), 6 μl of CP2-15C or CP2-9C primer (2.88 μM ...
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bioRxiv - Molecular Biology 2019Quote: ... primary or re-amplified WTA products) added to 1 μl Expand Long Template Buffer 2 (Expand Long Template PCR System, Roche Diagnostics), 1 μl dNTPs (10 mM each) ...
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bioRxiv - Molecular Biology 2020Quote: ... and UCRS Fwd and SF Rev respectively (Table S1) in PCR reactions with either KAPA HiFi DNA polymerase with high GC buffers (KAPA Biosystems) or Hot FIREPol Blend Master mix (Solis BioDyne) ...
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bioRxiv - Molecular Biology 2020Quote: ... a partial CYP6P9a upstream region was amplified in a final volume of 15μl PCR mixture contained 1.5μl of 10X Kapa Taq buffer A (Kapa Biosystems, Wilmington, MA, USA), 0.12μl of 5 U/μl KAPA taq ...
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bioRxiv - Genetics 2019Quote: ... of each library was used for bar-coding amplification using dual-barcoded single-stranded library adapters44 as primers in the following 100 μl volume reaction: 10 μl 10x PCR Buffer + MgCl2 (Roche, Basel, Switzerland) 0.4 μM of each primer ...
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bioRxiv - Immunology 2020Quote: ... generation of TCR libraries and generation of amplicons for deep sequencing were performed using the KAPA HiFi PCR kit with GC buffer (Roche Diagnostics, #07958846001) and custom designed primers (Supplementary Table 6) ...
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bioRxiv - Molecular Biology 2024Quote: ... treated with lysis buffer (RIPA buffer, 1x Roche Complete Protease Inhibitor cocktail ...
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bioRxiv - Microbiology 2021Quote: ... buffer (Roche). After collection ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR was performed using KAPAHiFi PCR HotStart Readymix (Roche) with an annealing temperature of 50° and an extension of 15 s for 30 cycles (melting temperature of one of two primers is 47°) ...
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bioRxiv - Genetics 2019Quote: ... PCR was conducted using FastStart PCR Master Mix (Roche), purified using the QIAquick PCR purification kit (QIAGEN ...
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bioRxiv - Neuroscience 2020Quote: ... PCR was performed using GC-RICH PCR System (Roche). SYNGAP primers (rSG_F ...
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bioRxiv - Neuroscience 2021Quote: ... PCR amplification using a HiFi PCR kit (Kapa Biosystems) and SINGV6 primer was performed with a modified thermocycling protocol (−98°C for 3 min ...
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bioRxiv - Cell Biology 2023Quote: ... and lysed in lysis buffer (RIPA buffer, 1x Roche Complete Protease inhibitor cocktail ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using the SYBR Green PCR Master Mix (Roche) on a ViiA7 Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Genomics 2020Quote: ... PCRs were performed using KAPA2G Robust PCR kits (KAPA Biosystems). A typical 25 μl reaction used 20–100 ng of genomic DNA ...
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bioRxiv - Plant Biology 2020Quote: ... PCR used the Kapa Taq PCR kit from Kapa Biosystems (Wilmington ...
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bioRxiv - Systems Biology 2021Quote: ... Barcoding PCR was done by KAPA HiFi PCR Kit (Roche) in a final 25μl reaction (11.5μl H2O ...
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bioRxiv - Developmental Biology 2020Quote: ... The PCR kit used was FastStrat PCR Master (Roche, 04710436001). The amplification product from mutated allele has a 341bp size and the one obtained from a wild-type allele is 262bp ...
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bioRxiv - Cancer Biology 2023Quote: ... Barcoding PCR was done using KAPA HiFi PCR Kit (Roche) in a final volume of 25 μl ...
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bioRxiv - Neuroscience 2023Quote: ... RT-PCR was achieved using 2X Faststart PCR mastermix (Roche) using primer sets and cycling conditions described in Supplementary Table 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... and blocked with TNB buffer (TN buffer + Blocking Reagent [Roche]) for 1 hour at room temperature ...
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bioRxiv - Genetics 2020Quote: ... PCR KIT (Roche). For each reaction ...
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bioRxiv - Biochemistry 2022Quote: ... RT buffer (Roche) and Reverse Transcriptase (Roche ...