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Citations for Roche :
1 - 50 of 711 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... nuclei were harvested as above and the nuclear pellet resuspended in 100 µl extraction buffer (3mM EDTA, 0.2mM EGTA and protease inhibitors (Roche)) ...
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bioRxiv - Genetics 2021Quote: Total proteins were extracted using protein extraction reagents (Roche Diagnostics Ltd., Shanghai, China) from the myocardial tissue of rats ...
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bioRxiv - Molecular Biology 2021Quote: ... For protein extraction cells were resuspended in protein extraction buffer (1xPBS, 10% glycerol, 1mM EDTA, 1mM PMSF, 1x Roche cOmplete™Protease Inhibitor Cocktail), and lysed in a bead beater ...
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bioRxiv - Plant Biology 2020Quote: ... Total proteins were extracted in the extraction buffer containing protease inhibitor cocktail (Roche) as described above ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Nuclear staining was performed using DAPI (Roche). Fluorescence images were acquired using a Zeiss LSM880 confocal microscope ...
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bioRxiv - Immunology 2023Quote: ... Stimulated cultures were lysed in M-PER mammalian protein extraction reagent supplemented with PhosphoSTOP (Roche) and cOmplete Tablets (Roche ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The protein extraction was performed by adding 10 M urea containing complete protease inhibitor cocktail (Roche) and Halt™ Phosphatase Inhibitor (Thermo ...
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bioRxiv - Neuroscience 2022Quote: ... and homogenized in a T-PER tissue protein extraction reagent supplemented with protease (Roche Applied Science) and phosphatase inhibitors (Millipore) ...
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bioRxiv - Molecular Biology 2020Quote: ... (a) Proteins were extracted from cryogrinded cell powders with different extraction solutions supplemented with protease inhibitors (Roche) (solution compositions listed in Supplementary Table 1) ...
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bioRxiv - Cell Biology 2023Quote: Testis protein extraction was performed in RIPA buffer with a cocktail of protease inhibitors (Roche cat. 11836170001), which was freshly added before beginning protein extraction ...
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bioRxiv - Physiology 2023Quote: ... cells/tissue were lysed in mammalian protein extraction reagent (M-PER) supplemented with protease inhibitor cocktail (Roche). Soluble proteins and insoluble matrix were separated by centrifugation at 10,000 × g ...
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bioRxiv - Immunology 2019Quote: ... These cells were then lysed in T-PER tissue protein extraction reagent (Pierce) containing protease inhibitor cocktail (Roche) and phosphatase inhibitor cocktail 1(Sigma) ...
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bioRxiv - Immunology 2022Quote: Protein extraction was performed using RIPA buffer in the presence of complete Mini EDTA-free protease inhibitor (Roche) and PhosSTOPTM phosphatase inhibitor (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... the frozen brain samples were homogenized in tissue protein extraction reagent (Pierce) supplemented with complete protease inhibitors (Roche), and centrifuged for 1 hour at 430 000 g at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: Proteins were extracted using Tris-buffered Saline (TBS) with 0.5% NP40 protein extraction buffer containing protease and phosphatase inhibitors (Roche), on ice ...
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bioRxiv - Plant Biology 2023Quote: ... Meristem-enriched samples or whole seedlings were ground in liquid nitrogen and homogenized in cold protein extraction buffer (50 mM Tris HCl pH 7.5, 10% glycerol, 1 mM DTT, 1% IGEPAL, 1× Roche EDTA-free protease inhibitor and 1× Roche phosphatase inhibitor) ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in extraction buffer (SDS 2%, 50 mM Tris-Cl pH 7.4, 1 mM PMSF, 2x cOmplete protein inhibitor cocktail (Roche)) ...
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bioRxiv - Cancer Biology 2024Quote: ... or protein extraction (50 mM Tris-HCl, 1% NP40, 0.15 M NaCl, pH 8) supplemented with phosphatase (PhoStop, Roche) and protease inhibitors (Complete ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA extraction was performed by using the HighPure FFPET RNA extraction kit (Roche). RNA concentration and quality were assessed on the Agilent Bioanalyzer ...
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bioRxiv - Molecular Biology 2020Quote: ... Then cells were lysed in B-per protein extraction reagent 4 ml/ gram bacterial pellet in the presence of cOMPLETE mini protease inhibitor cocktail (Roche) for 15 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... MSNs were harvested in M-PER Mammalian Protein Extraction Reagent (Pierce) with cOmplete Mini EDTA-free protease inhibitor (1 tablet/10 mL) (Roche). Whole-cell lysates were sonicated with a 5 second pulse followed by a 5 second rest (X 5 times ...
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bioRxiv - Cancer Biology 2020Quote: Protein extracts were prepared from drug-treated cells by lysis on ice for 20 minutes in M-PER Mammalian Protein Extraction Reagent (ThermoScientific) supplemented with protease and phosphatase inhibitors (Roche). Lysates were centrifuged at 16,000 RPM for 20 minutes at 4°C and the supernatants were assessed for concentration via a Bradford assay ...
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bioRxiv - Microbiology 2020Quote: Further protein extraction steps for each of 10 samples were carried out in solutions supplemented with cOmplete protease inhibitors (Roche) and PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... At DIV 17 cells were washed with ice-cold PBS and immediately extracted with ice-cold T-PER protein extraction buffer (Pierce) supplemented with inhibitors for proteases and phosphatases (Roche). The lysates were centrifuged at 15000 g for 15 minutes at 4°C and the supernatants were used for further analysis.
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bioRxiv - Microbiology 2020Quote: Western blot analysis of infected and uninfected erythrocytic material performed on protein extracts prepared by resuspending cells in M-PER mammalian protein extraction reagent (Pierce) supplemented with 1x protease inhibitory cocktail (EDTA free) (Roche), 300 mM benzamidine ...
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bioRxiv - Molecular Biology 2023Quote: ... The extraction of total proteins was performed using the PIERCE RIPA buffer (Thermo, #89900) complemented with EDTA-free cOmplete PIC (ROCHE).
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bioRxiv - Genomics 2019Quote: ... were bound to nuclear extract prior to poly d(I-C) (Roche) incubation at 4°C for 1 hr.
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei lysates (in Nuclear Buffer Lysis 3, supplemented with protease (Roche, 11697498001) and phosphatase inhibitors (1 mM Na3O4V (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed in M-PER mammalian protein extraction reagent (Thermo, MA, USA) with protease and phosphatase inhibitors (Roche, Basel, Switzerland), and proteins were quantified using protein assays (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2019Quote: ... cells were incubated for 48 h at 37°C in 5% CO2, harvested and lysed using M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, 78501) (with 1X Roche Complete Protease Inhibitor and 100 mM NaCl) ...
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bioRxiv - Cell Biology 2020Quote: ... The biotinylated protein was incubated with 50ug fresh mitochondria extraction in import buffer containing ATP regeneration system (Creatine kinase (Roche 10127566001), Creatine Phosphate (Sigma-Aldrich 10621714001 ...
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bioRxiv - Bioengineering 2023Quote: ... lung tissues were thawed on ice and then resuspended in 0.5ml of Tissue Protein Extraction Reagent (T-PER) (ThermoScientific, cat# 78510) containing Complete Mini Protease Inhibitor Cocktail (Roche, cat# 11836170001). Tissues were homogenized using an electric handheld tissue homogenizer and were stored at -80°C until used for ELISA.
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bioRxiv - Cancer Biology 2020Quote: ... protein lysates were prepared from HCC1428 cells with control or E2-E10 siRNA knockdown using modified Tissue Protein Extraction Reagent (TPER) (Pierce) and a cocktail of protease and phosphatase inhibitors (Roche Life Science). The protein lysates were then diluted into 0.5 mg/ml of total protein in SDS sample buffer and denatured on the same day ...
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bioRxiv - Neuroscience 2020Quote: Cells from mouse brain or spleen tissues were lysed with T-PER Tissue Protein Extraction Reagent (Thermo) containing Complete Protease Inhibitor Cocktail (Roche Applied Science). Protein concentrations were determined with BCA protein assay reagents (Pierce) ...
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bioRxiv - Molecular Biology 2022Quote: Mouse liver tissues and cells were homogenized and lysed in Protein Extraction Reagent (P0013, Beyotime Biotechnology) supplemented with protease inhibitor cocktail (04693159001, Roche, Basel, Switzerland). Equal amounts of protein lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membranes (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... Bacterial pellets were lysed in 10 ml B-PER Bacterial Protein Extraction Reagent (Pierce, Rockford, ILL) with Complete Protease Inhibitor Cocktail Tablets (Roche, Indianapolis, IN). Extracts were cleared by centrifugation ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclear labeling was completed by incubating slices with DAPI (0.1 μg/mL, Roche) at room temperature for 5 min after incubation with secondary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclear labeling was completed by incubating slices with DAPI (0.1 μg/mL, Roche) at RT for 5 min after incubation with secondary antibodies ...
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bioRxiv - Bioengineering 2020Quote: ... Before extraction protease inhibitor (Roche 11873580001), phosphatase inhibitor cocktail 3 (p2850 ...
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bioRxiv - Plant Biology 2021Quote: ... total protein was isolated from Agrobacterium infiltrated leaves using extraction buffer (50mM Tris-Cl, 1% β-Mercaptoethanol and protease inhibitor cocktail (Roche, Basel, Switzerland)) ...
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bioRxiv - Developmental Biology 2022Quote: Testes from adult mice were cut into ~20 mg pieces and transferred into ~200 μl pre-cooled denaturing buffer from the Minute Total Protein Extraction Kit (SD-001, Invent Biotech, Plymouth, MN, USA) containing protease inhibitors (CO-RO, Roche, Indianapolis, IN, USA) and phosphatase inhibitors (PHOSS-RO ...
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bioRxiv - Biochemistry 2022Quote: ... Hela nuclear extract (Ipracell Ltd) in binding buffer supplemented with protease inhibitors (Roche Ltd) was added to the immobilized proteins ...
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bioRxiv - Microbiology 2020Quote: ... with RNA extraction controls—5 μL of LightMix® Modular EAV RNA Extraction Control (EAV; Roche, Basel, Switzerland) or 10 μL of MS2 phage (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: Synaptosomes were prepared using the Procedure for Synaptic Protein Extraction from Neuronal Tissue” and Syn-PER Synaptic Protein Extraction Reagent (Thermo Fisher, #87793, containing a half tablet of cOmplete™ Protease Inhibitor Cocktail (Roche, #CO-RO) per 12.5 ml Syn-PER reagent ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclear pellets were washed once with 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) (Roche, 10236276001) in methanol (1 μg/ml) ...
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bioRxiv - Cell Biology 2020Quote: ... second extraction buffer was supplemented with DNaseI (Roche) to a final concentration of 100μg/ml.
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bioRxiv - Neuroscience 2021Quote: ... nuclear staining was performed by adding 1 μg/mL DAPI in the buffer solution (10236276001, Roche Diagnostics). After incubation ...
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bioRxiv - Molecular Biology 2022Quote: ... The nuclear pellet was washed gently with 10 volumes of Buffer A containing 1x CPI (Roche, #11836145001), 0.5 mM DTT and 0.15 % NP40 and centrifuged for 5 min at 3,200 g at 4°C min ...
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bioRxiv - Genetics 2020Quote: ... A small portion of the vitelline glands from the posterior part of each fluke was used for DNA extraction using the High Pure DNA Extraction Kit (Roche, Mannheim, Germany) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2019Quote: ... Feature extraction was performed with NimbleScan v2.6 (Roche NimbleGen) by the MAD (University of Amsterdam ...