Labshake search
Citations for Roche :
1 - 50 of 1681 citations for Integrin Alpha M CD11b ITGAM Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... cells were lysed in 8 M urea buffer (8 M urea, 1 M Tris pH 7.4, 5 M NaCl) containing protease and phosphatase inhibitors (Roche) followed by sonication at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were incubated in staining solution (1 M Tris-HCl pH 9.5, 5 M NaCl, 1 M MgCl2, NBT-BCIP, H2O) (Roche) in the dark for 10 min at room temperature ...
-
bioRxiv - Immunology 2021Quote: Cells were collected in RIPA buffer (0.5 M EDTA, 1 M Tris-Cl pH7.5, 1 M NaCl, 200 mM, Roche protease inhibitor) at 0h ...
-
bioRxiv - Microbiology 2020Quote: ... Pegylated interferon alpha-2a (PEG-IFN-α; Pegasys, 90 mcg, Roche) was aliquoted and stored at room temperature until further use ...
-
bioRxiv - Neuroscience 2020Quote: ... Forebrains were finely chopped and then homogenized in 0.32 M sucrose solution (0.32 M sucrose, 10 mM Hepes pH 7.4, with cOmplete protease (Roche) and phosphatase inhibitors ...
-
bioRxiv - Bioengineering 2023Quote: ... 3 % NaCl (5 M, v/v), 0.08 % CaCl2 (2.5 M, v/v) and cOmpleteTM ULTRA Protease Inhibitor Cocktail tablets (Roche) in H2O ...
-
bioRxiv - Plant Biology 2024Quote: ... 0.5 M EDTA and Protease Inhibitor (Roche). The cell lysate was collected by centrifuging the homogenate at 20,000g for 10 min ...
-
bioRxiv - Genetics 2020Quote: ... Cells pellets were combined and lysed in a denaturing urea buffer (8 M urea, 0.1 M ammonium bicarbonate, pH 8, 150 mM NaCl, 1 Roche mini protease inhibitor tablet without EDTA/10 ml ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 1.5 ml 5 M NaCl, 12.5 μL 2 M Spermidine, final volume brought to 50 ml with dH2O, complemented with one Roche Complete Protease Inhibitor EDTA-Free tablet) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The remaining supernatant was diluted in 1 mL pre-warmed capturing buffer (0.5 M LiCl, 4 M urea, 100U Ribolock inhibitor, Thermo, 1x protease/phosphatase inhibitor, Roche) and 10 µL from an equimolar stock (10 µM ...
-
bioRxiv - Genetics 2021Quote: ... extracts were incubated 1h at 4°C with 50 µL magnetic beads (Dynabeads M-280 sheep anti-mouse IgG ,invitrogen 11202D) coated with anti-GFP antibodies (Roche ref:11814460001). Proteins extracts were resolved on 4-15% polyacrylamide gels ...
-
bioRxiv - Neuroscience 2019Quote: ... The resulting supernatant is the soluble fraction and the insoluble fraction is generated by resuspending the pellet in urea buffer (30 mM Tris, 7 M Urea, 2 M Thiourea, 4% CHAPS, 1X Protease Inhibitor Cocktail (Roche), 0.5 mM PMSF ...
-
bioRxiv - Neuroscience 2022Quote: ... Cell pellets were homogenized in lysis buffer (7 M urea, 2 M thiourea, 1% CHAPS, 70 mM DTT, and Roche EDTA-free complete protease inhibitor cocktail ...
-
bioRxiv - Biochemistry 2021Quote: ... 8 M urea and protease inhibitor (Roche, 05892791001) with sonication on ice ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.05 M TRIS-HCl (Roche Cat. No. 10812846001), 0.8 mM EDTA (Sigma Aldrich Cat No ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.25 M KCl) supplemented with protease inhibitors (Roche) and sonicated 3x5 seconds using a Bioruptor (Diagenode) ...
-
bioRxiv - Plant Biology 2023Quote: ... 0.2 mM EDTA, 0.3 M NaCl, 1 M Urea, 1% IGEPAL CA-630, 1 mM PMSF, and Roche protease inhibitor cocktail) was added ...
-
bioRxiv - Molecular Biology 2023Quote: ... DIG-labeled TER and alpha-tubulin probes were generated using the PCR DIG Probe Synthesis Kit (Roche) and used in the hybridization step (S1 Table ...
-
bioRxiv - Genomics 2024Quote: ... The probe was labeled with alpha-32P CTP using the High Prime DNA labeling kit (Roche, #11585584001). The labelled probe was purified on a G50 sephadex column (Cytiva ...
-
bioRxiv - Cancer Biology 2022Quote: ... Initial T cell activation was performed with anti-CD3/anti-CD28 microbeads (T Cell TransAct, Miltenyi Biotech, 130-111-160) and 10µg/ml Phytohemagglutinin-M (PHA-M) (Roche 11082132001, 20 mg). Lentivirus transduction procedure ...
-
bioRxiv - Molecular Biology 2022Quote: ... The cells were pelleted and resuspended in 2× SDS-PAGE solvent (4% SDS, 20% glycerol, 0.1 M DTT, 0.125 M Tris-HCl pH 7.5 and Roche EDTA-free protease inhibitor cocktail) and boiled at 95°C for 5min ...
-
bioRxiv - Biophysics 2020Quote: ... EDTA-free lysis-M buffer with protease inhibitor (Roche). Protein concentrations were determined using PierceTM BCA kit (Thermo ScientififTM) ...
-
bioRxiv - Neuroscience 2021Quote: ... 0.15 M NaCl and Complete protease inhibitor cocktail (Roche)] ...
-
bioRxiv - Immunology 2021Quote: ... hydrocortisone hemisuccinate (7×10−5 M, Roche-Boehringer-Manheim) and FCS (10% selected ...
-
bioRxiv - Neuroscience 2019Quote: ... 0.15 M NaCl and complete protease inhibitor cocktail (Roche) by ultrasonication ...
-
bioRxiv - Cell Biology 2023Quote: ... 0.3 M sucrose containing Complete Protease Inhibitor Cocktail (Roche). Suspended cells were homogenized using a syringe with a 27-gauge needle ...
-
bioRxiv - Immunology 2023Quote: ... Initial T cell activation was performed using anti-CD3/anti-CD28 microbeads (T Cell TransAct, Miltenyi Biotech, 130-111-160) and 10 μg/mL Phytohemagglutinin-M (PHA-M) (Roche 11082132001, 20 mg). Lentiviral transduction was performed by inoculating cells with Vectofusin 1 (10 μg/mL ...
-
bioRxiv - Microbiology 2020Quote: ... 0.5 M β-mercaptoethanol) supplemented with protease inhibitor cocktail (Roche) and sonicated for 10 s at 20% amplitude using a Sonics Vibra Cell sonicator fitted with a microprobe ...
-
bioRxiv - Biophysics 2022Quote: ... 0.5 M sucrose) supplemented with one protease inhibitor tablet (Roche). Osmotic shock was performed by the addition of diluted TES buffer to release the periplasmic proteins ...
-
bioRxiv - Biophysics 2022Quote: ... 0.5 M sucrose) supplemented with one protease inhibitor tablet (Roche). Osmotic shock was performed by the addition of diluted TES buffer to release the periplasmic proteins ...
-
bioRxiv - Biophysics 2022Quote: ... 0.02% NaN3 and 1x cOmpleteT M Protease Inhibitor Cocktail (Roche). 10% D2O was added for lock.
-
bioRxiv - Microbiology 2021Quote: ... 2 M CaCl2 and 2 UU/ml DNAse I (Roche) for 30 min at 37°C ...
-
bioRxiv - Microbiology 2023Quote: ... and suspended in 8 M urea containing PhosSTOP (Roche Diagnostics) and Benzonase (Novagen) ...
-
bioRxiv - Microbiology 2023Quote: ... 0.5 M β-mercaptoethanol) supplemented with protease inhibitor cocktail (Roche) and lysates were sonicated for 10 s at 20% amplitude using a Vibra-Cell sonicator (Sonics ...
-
bioRxiv - Microbiology 2022Quote: ... At the end of the cell disruption dry homogenised powder of cells were resuspended in 1 ml urea buffer (8 M urea, 50 mM Tris/HCl pH 8.0 containing one tablet of ‘cOmplete’ protease inhibitor per 25 ml, Roche, Germany; 1 mM DTT; 0.1 M PMSF) and centrifuged at 16,000 × g for 10 minutes at 4 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... 2 M NaCl with Complete protease inhibitor tablet (Roche, Indianapolis, IN) and centrifuged for 30 min at 13,000 rpm 4°C to pellet cell debris ...
-
bioRxiv - Neuroscience 2021Quote: ... 0.32 M sucrose supplemented with Complete mini protease inhibitors (# 11836153001, Roche), PhosStop phosphatase inhibitor cocktail (# 4906837001 ...
-
bioRxiv - Neuroscience 2020Quote: ... 0.25 M sucrose and EDTA-free protease inhibitors (Roche, cat# 05056489001) followed by centrifugation at 9000 rpm for 15 minutes ...
-
bioRxiv - Cell Biology 2022Quote: Cell lysates were prepared using cOmplete™ Lysis-M (4719956001, Roche) with protease and phosphatase inhibitors and subjected to standard electrophoresis and transfer as previously described41 ...
-
bioRxiv - Neuroscience 2024Quote: ... 0.01 M Tris-HCl pH 7.4] containing protease inhibitors (Roche, Switzerland). For sarkosyl preparations ...
-
bioRxiv - Molecular Biology 2021Quote: Serum creatinine was determined using the creatininase/creatinase specific enzymatic method described by Bömer using a commercial kit (Alpha Laboratories Ltd. Eastleigh, UK) adapted for use on a Cobas Fara centrifugal analyser (Roche Diagnostics Ltd ...
-
bioRxiv - Molecular Biology 2021Quote: Serum albumin measurements were determined using a commercial serum albumin kit (Alpha Laboratories Ltd., Eastleigh, UK) adapted for use on Cobas Mira analyser (Roche Diagnostics Ltd ...
-
bioRxiv - Molecular Biology 2021Quote: ... HCXII-0155) was incubated with neutrophils that were pre-treated with vehicle or DNase I (10U/ml, Dornase alpha, Roche, Germany) for 1h ...
-
bioRxiv - Immunology 2024Quote: ... the cells were quickly transferred into 10 ml of pre-warmed RPMI supplemented with 10% FBS and 100µg of Pulmozyme (dornase alpha, Roche, Basel, Switzerland) for 30 min at 37°C ...
-
bioRxiv - Neuroscience 2022Quote: ... pH 7.4 plus 0.32 M sucrose supplemented with protease inhibitor cocktail (Roche). To isolate synapse-enriched fractions ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 m DTT) supplemented with protease inhi itor ta lets (Roche, 11697498001) and 75 U/ml of Pierce universal nuclease (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: ... 8 M Urea loading dye was supplemented with complete protease inhibitor (Roche). 100 μl Urea loading dye were used to resuspend cell pellet after NaOH treatment ...
-
bioRxiv - Developmental Biology 2020Quote: ... Extracts were made using Complete Lysis-M EDTA-Free Lysis buffer (Roche). Antibody Array was performed ...
-
bioRxiv - Biochemistry 2024Quote: ... 8 M Urea and 1x cOmpleteTM EDTA-free protease inhibitor (Roche #11873580001). Lysates were sonicated with a probe sonicator and cleared via centrifugation at 15,000x g for 30 min ...
-
bioRxiv - Neuroscience 2023Quote: ... alpha synuclein and NfL) were measured in both cohorts using Elecsys assays in accordance with the manufacturer’s instructions (Roche Diagnostics International Ltd).52 CSF analyses were performed by technicians blinded to all clinical and imaging data ...