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Citations for Roche :
1 - 50 of 700 citations for Hrms Rapid Pcb Screening Calibration Solution Cs0.05 Unlabeled 13C12 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... HRM reaction was prepared using HRM master mix (Roche Diagnostics, Mannheim, Germany) and the PCR was carried out with LightCycler 480 instrument (Roche Diagnostics ...
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Establishment of Wolbachia strain wAlbB in Malaysian populations of Aedes aegypti for dengue controlbioRxiv - Microbiology 2019Quote: ... 0.25 µL HRM Master (Roche), 0.064 µL dNTPs (25 mM ...
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bioRxiv - Genetics 2023Quote: ... PCR for HRM analysis was performed by using a KAPA HRM Fast PCR Kit (Roche, Basel, Switzerland) on a LightCycler 96 System (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... 1X Rapid DNA Ligation buffer (Rapid DNA Ligation Kit, Roche 11635379001), 1X Dilution buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.003mM CTP (unlabeled rNTPs from Roche 11277057001), 0.5mM Bromo-UTP (Sigma B7166) ...
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bioRxiv - Zoology 2020Quote: ... The library quantity was determined by qPCR using the rapid library standard quantification solution and calculator (Roche). The libraries were sequenced on the Illumina HiSeq platform.
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bioRxiv - Neuroscience 2021Quote: ... and reaction mixtures were: 1X LightCycler 480 HRM master mix (Roche), 2mM MgCl2 ...
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bioRxiv - Neuroscience 2021Quote: ... The HRM analysis was done on the LightCycler 480 instrument (Roche), and reaction mixtures were ...
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bioRxiv - Systems Biology 2020Quote: ... ligation (Rapid DNA Ligation Kit, Roche) was performed to obtain the two final vector backbones (4299 bp) ...
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bioRxiv - Neuroscience 2019Quote: ... using the following reaction mixtures: 1× LightCycler 480 HRM master mix (Roche), 2mM MgCl2 ...
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bioRxiv - Neuroscience 2019Quote: ... The HRM analysis was performed on a LightCycler® 480 instrument (Roche) using the following reaction mixtures ...
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bioRxiv - Microbiology 2021Quote: The HRM curve analysis was performed using a LightCycler 480 Instrument (Roche) with the AccuMelt HRM SuperMix fluorescent dye (DNAture ...
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bioRxiv - Cell Biology 2022Quote: ... the adjusted hRM preps were further treated with S7 micrococcal nuclease (Roche, Germany) at a concentration of 0.075 U/μl in the presence of 0.33 mM CaCl2 for 6 min in a 25°C water bath ...
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bioRxiv - Immunology 2024Quote: ... unlabeled streptavidin was prepared by incubating 190 µg streptavidin (Roche, Basel, Switzerland) in the presence of a 100-fold molar excess of biotin in 1X Gibco PBS in a final volume of 250 μl ...
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bioRxiv - Plant Biology 2020Quote: ... HRM analysis was performed using the High Resolution Melting Master (Roche Applied Science, Germany) on the LightCycler® 480 II system (Roche Applied Science ...
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bioRxiv - Physiology 2024Quote: ... HRM analysis was performed on the LightCycler 480 System II (Roche Diagnostics, Basel, Switzerland) using the LCGreen Plus dye (BioFire Defense ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA libraries were prepared using KAPA RNA HyperPrep Kit with RiboErase (HRM) (Roche KK8651). Briefly ...
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bioRxiv - Plant Biology 2019Quote: ... and the Rapid DNA Dephos & Ligation Kit (Roche) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The HRM assay was performed with LightCycler® 480 System II (Roche Life Science, Germany). The HRM data was analyzed using the Melt Curve Genotyping and Gene Scanning Software (Roche Life Science ...
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bioRxiv - Genetics 2022Quote: ... PCR was performed in 12 μL containing 6 μL Kapa HRM-Fast Master mix (Roche), supplemented with 1.5 mM MgCl2 ...
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bioRxiv - Molecular Biology 2024Quote: ... and then dephosphorylated using the alkaline phosphatase rAPid (Roche). A target-specific gRNA was designed using CRIPR-P 2.0 (http://crispr.hzau.edu.cn/CRISPR2) ...
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bioRxiv - Immunology 2023Quote: ... digested and purified plasmid was dephosphorylated (rAPid Alkaline Phosphatase) (Roche) and used in a Gibson Assembly reaction together with the purified PCR products ...
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bioRxiv - Cancer Biology 2022Quote: ... For ligations using the Rapid DNA Ligation Kit (Roche 11635379001) vector and insert were mixed in a 1:3 molar ratio ...
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bioRxiv - Cell Biology 2020Quote: ... and subsequent ligation (Rapid DNA Ligation Kit, Roche, Merck, Zug, CH). Then ...
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bioRxiv - Plant Biology 2020Quote: ... The PCR and HRM analysis were performed in a LightCycler® 480 system II (Roche Life Science, Germany) using a program consisting of an initial denaturation at 95°C for 5 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... The HRM data was analyzed using the Melt Curve Genotyping and Gene Scanning Software (Roche Life Science, Germany). Analysis of HRM variants was based on differences in the shape of the melting curves and in Tm values.
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bioRxiv - Microbiology 2024Quote: ... and DNA ligations were performed using the Rapid DNA Ligation kit (Roche).
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bioRxiv - Plant Biology 2022Quote: ... The identified cdkd;3-3 mutation was genotyped by the High-Resolution Melting (HRM) qPCR-based method using primers described in Table S3 using Lightcycler96 thermocycler (Roche) using the inbuilt HRM profile.
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bioRxiv - Microbiology 2023Quote: ... We recircularized linearized plasmid PCR products with a Rapid DNA Ligation Kit (Roche) and transformed plasmids into DH5α-competent cells (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... Prepared insert and vector fragments were ligated using Rapid DNA Ligation Kit (Roche), transformed to One Shot TOP10 Chemically Competent E ...
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bioRxiv - Cell Biology 2022Quote: ... Two different HRMA-compatible platforms were used (Applied Biosystems ViiA 7 Real-Time PCR System, using the MeltDoctor HRM Master Mix, and Roche LightCycler 480 System ...
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bioRxiv - Microbiology 2022Quote: ... were determined using the Rapid amplification of cDNA-5’ends (5’RACE) kit (Roche). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... We re-circularized linearized plasmid PCR products with a Rapid DNA Ligation Kit (Roche) and transformed plasmids into DH5α-competent cells (Thermo Fisher) ...
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Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: ... and transferred into pLXINmod using EcoR1 and BamH1 and Rapid DNA Ligation Kit (11635379001, Roche). pLXINmod is a modified retroviral vector based on pLXIN but with an extended multiple cloning site ...
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bioRxiv - Genetics 2023Quote: ... AB individuals with the shortest wild introgressed fragment were selfed and the progeny was genotyped by High Resolution Melting (HRM) on a LightCycler 480 Real-Time PCR (Roche Diagnostics, Meylan, France) to identify recombinants with shorter introgressions ...
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bioRxiv - Immunology 2021Quote: ... also available at addgene (plasmid 15912) (http://www.addgene.org/15912/) using the Roche Rapid ligation kit (Roche). The correct insert orientation was confirmed by NheI and SmadI (NEB ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryonic tail biopsies were used for rapid genotyping using KAPA Mouse Genotyping Kits (Kapa Biosystems, KK7352). All mouse protocols and experiments were approved by the Rutgers Institutional Animal Care and Use Committee ...
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bioRxiv - Genetics 2021Quote: ... digested with NdeI and EcoRI using a 3-way ligation reaction (Rapid DNA ligation kit, Roche). This generated the intermediate plasmid pCMV-BE2+dCas9m4 ...
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bioRxiv - Microbiology 2024Quote: The rapid translation system has been performed according to the producer’s protocol with slight modifications (Roche, Rapid Translation System RTS 100 ...
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bioRxiv - Cell Biology 2022Quote: ... pLPCX vector was digested and then dephosphorylated before ligation using the Rapid DNA Dephos & Ligation Kit (Roche). Ampicillin resistance clones were verified with 5’ sequencing primer 5’-AGCTGGTTTAGTGAACCGTCAGATC-3’ and 3’ sequencing primer 5’-ACCTACAGGTGGGGTCTTTCATTCCC-3’ ...
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bioRxiv - Neuroscience 2024Quote: ... Linear fragments were then re-ligated using the Rapid DNA Dephosphorylation and Ligation Kit (Roche, Millipore Sigma): dephosphorylating the backbone fragment and ligating at room temperature for at least fifteen minutes.
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bioRxiv - Synthetic Biology 2022Quote: ... The calibration curve samples and the aliquots of working standard were then tested using a cobas® 6800 system (Roche) and cobas® HBV Test (Roche ...
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bioRxiv - Genomics 2023Quote: ... and analyzed on a light cycler rapid thermal system (LightCycler®480 2.0 Real time PCR system, Roche). ChIP-qPCR results were normalized on input signal (% input) ...
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bioRxiv - Molecular Biology 2023Quote: Successful virus rescue was monitored with SARS-CoV-2 Rapid Antigen Test (Cat# 9901-NCOV-01G, SD BIOSENSOR, Roche) by applying 100 μL of unfiltered culture supernatant directly onto the test device.
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bioRxiv - Physiology 2019Quote: ... HbA1c was measured by finger-stick in the same manner with a rapid HbA1c device (Accu-chek, Roche, Indianapolis, IN). Adipose tissue thickness over the forearm muscle was measured with B-mode ultrasound (GE Logic Q ...
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bioRxiv - Physiology 2020Quote: 100 µL of whole blood was pelleted by rapid centrifugation and pellets were lysed in 100 µL of RIPA lysis buffer with proteinase inhibitor cocktail (Roche). Following an additional centrifugation step ...
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bioRxiv - Genomics 2023Quote: ... supplemented with 0.2μM of specific primer pairs (Table S9) and run on a light cycler rapid thermal system (LightCycler®480 2.0 Real time PCR system, Roche) with 20 sec of denaturation at 95°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Phosphorylated and annealed oligos were diluted 1:10 and a Golden Gate reaction was setup with 1x rapid ligase (Roche), 10 units of BbsI enzyme ...
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bioRxiv - Neuroscience 2021Quote: ... was used to maintain the temperature of the water between the objective lens and cranial window at 36.6 °C to avoid the objective acting as a heat sink and to comply with the Oxyphor 2P calibration temperature (Li et al 2019, Podgorski & Ranganathan 2016, Roche et al 2019).
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bioRxiv - Microbiology 2022Quote: ... with the IDT E Assay First Line Screening primers and probe (Cat # 10006804) on a LightCycler 96 System (Roche, Cat# 05815916001).