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Citations for Roche :
1 - 50 of 641 citations for Glucosamine N acetyl 6 Sulfatase GNS Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... uridine 5’-diphospho-N-acetyl-glucosamine (UDP-GlcNAc) and cytidine-5’-monophospho-N-acetylneuraminic acid (CMP-Neu5Ac) were obtained from Roche Diagnostics [UDP-Gal ...
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bioRxiv - Molecular Biology 2021Quote: ... lysate supernatant from cells expressing N-terminal 6-His-tagged proteins were incubated with nickel-nitrilotriacetic acid (Ni-NTA) cOmplete His-tag purification resin (Roche) for 1 h at 4° C ...
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bioRxiv - Biochemistry 2022Quote: (His)6-GST-SNX15 MIT was bound to cOmplete His-Tag purification beads (5 mL, Roche, Germany, 2h) and washed with 2 L wash buffer ...
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Serine-ubiquitination regulates Golgi morphology and the secretory pathway upon Legionella infectionbioRxiv - Cell Biology 2020Quote: ... His (Roche), GRASP55 (Proteintech) ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was set by using qPCRBIO Probe Mix Hi-ROX (Nippongenetics) and TaqMan (5’: 6-FAM, 3’: TAMRA) or UPL (Universal Probe Library, Roche) probes ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK293 cells were transiently transfected with β-arrestin 2-Renilla luciferase 8 (Rluc8) and human C5aR2-Venus constructs using XTG9 (Roche, Sydney, Australia) for 24 h ...
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bioRxiv - Immunology 2021Quote: We used DOTAP (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate) (Roche) to introduce LPS or oxPLs into cells following the method by Zanoni et al (Zanoni et al. ...
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bioRxiv - Microbiology 2022Quote: ... 25 uL of N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methylsulfate (DOTAP) (Roche Diagnostics Deutschland GmbH ...
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bioRxiv - Cell Biology 2022Quote: ... and His-tag (11965085001, Roche) antibodies were used to detect the proteins.
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bioRxiv - Biochemistry 2019Quote: ... anti-His-tag (11922416001; Roche), anti-vinculin (V4505 ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1 mM ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA) and protease inhibitor cocktail (Roche) on ice for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... Two µg plasmid DNA and 25 µL DOTAP (N-[1-(2,3- Dioleoyloxy)propyl]-N,N,N-trimethylammonium methylsulfate) (Roche Diagnostics Deutschland GmbH ...
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bioRxiv - Developmental Biology 2021Quote: ... His-tagged proteins in soluble fraction were purified using cOmplete His-Tag Purification Columns (Roche). The columns were washed with 10 column volumes of wash buffer 1 (20 mM Tris ...
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bioRxiv - Neuroscience 2021Quote: ... Vector DNA was transiently transfected into human embryonic kidney (HEK) cells using Fugene-6 (Roche Molecular Biochemicals) transfection reagent ...
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bioRxiv - Immunology 2023Quote: ... Cationic lipid N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate (Dotap, Liposomal Transfection Reagent, Cat#1202375, Roche, Nonnenwald, Penzberg, Germany) was used for the conjugation of phosphorothioated miRNAs and Dotap-formulated miRNAs were used for in vitro delivery of miRNAs ...
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bioRxiv - Biochemistry 2021Quote: ... cOmplete His-Tag Purification Resin (Roche) was added to the supernatant and samples were incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Either His (Thermo) or GFP (Roche) or GST (Santa Cruz ...
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bioRxiv - Plant Biology 2020Quote: ... 4 µg recombinant NAA50 was incubated with 100 µM Acetyl-Coenzyme A (Roche) in a 2X acetylation buffer (50mM Tris HCl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... MEFs were seeded subconfluently o/n (unless indicated otherwise) onto coverslips acid-washed and coated with human fibronectin (25 μg/ml, #11051407001, Roche), as described (Dimchev and Rottner ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoblot with the anti-His-antibody conjugated to peroxidase (BMG-His-1 monoclonal antibody; Roche, Basel, Switzerland) was used to confirm the presence of enzymes.
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bioRxiv - Biophysics 2022Quote: ... the supernatants containing the His-tagged histones were combined with NiNTA resin (Complete His-Tag purification Resin, Roche) (1mL of Ni-NTA per 1L of bacteria ...
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bioRxiv - Neuroscience 2023Quote: ... prior to transfection with 2 μg pcDNA3.1 encoding N-terminally HA-tagged human 1N3R tau or 1N4R tau (14) using X-tremeGENE 9 (Roche Life Sciences), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... and 1/5000 anti-His (11667475001, Roche) for GFP and His-Tag detection ...
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bioRxiv - Immunology 2021Quote: ... N-Glycans were released by 10 U N-glycosidase F (Roche) digestion in 50 mM NH4HCO3 buffer pH 8.4 ...
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bioRxiv - Cancer Biology 2023Quote: ... For N-glycan release 2.5 U N-glycosidase F (Roche, DE) were added and the resulting mixture was incubated overnight at 37°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 8G8-Fab were expressed with His-tag and purified using the cOmplete His-Tag purification resin (Roche, USA) following standard protocols ...
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bioRxiv - Immunology 2021Quote: ... and 0.5% Zwittergent 3-12 (N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate) (pH 8.0) and the presence of Complete® protease inhibitor (Roche). The preparation was centrifuged for 10 min at 2500 rpm and 4 °C and supernatant was transferred to a new tube and centrifuged for 40 min at 30,000 x g and 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% Zwittergent 3-12 (N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate) and protease inhibitor (Complete, Roche Applied Science)) for 2 h at 0°C (ref A) ...
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bioRxiv - Biophysics 2020Quote: ... and amplified using KAPA Hi Fi polymerase (Roche) in 20 cycles of emulsion PCR35 (ePCR ...
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bioRxiv - Biochemistry 2022Quote: ... purified by cOmplete His-Tag purification columns (Roche), and concentrated to 1 mg/mL using Amicon Ultra 10,000 NMWL (Merck) ...
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bioRxiv - Microbiology 2020Quote: ... and cOmplete™ His-tag Purification Resin (Roche). Purity and glycosylation of recombinant proteins were examined by PNGase F (N-Zyme Scientifics ...
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bioRxiv - Developmental Biology 2022Quote: ... the cOmplete his-tag purification matrix from Roche is working equally well ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... Complete His-Tag Purification Resin (Roche, Basel, Switzerland), or Amylose Resin (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... 6.7 mM N,N-dimethylcyclohexylamine (DMCH) and EDTA-free complete protease inhibitor tablets (Roche). The mixture was incubated at 4°C with rotation for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... 12.5 μL of Kappa Hi Fi Hotstart ReadyMix (Roche) and each 0.75 μL (0.3 μM ...
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bioRxiv - Biochemistry 2020Quote: ... and incubated with cOmplete His-Tag purification resin (Roche) for 16 hours on a tube roller (Starlab ...
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bioRxiv - Cancer Biology 2021Quote: ... 12.5 μL of Kappa Hi Fi Hotstart ReadyMix (Roche) and each 0.75 μL (0.3 μM ...
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bioRxiv - Plant Biology 2020Quote: ... 12 µl of complete His-Tag Purification Resin (Roche) was added and incubated for 15 min at 25°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we used Kapa Hi-Fidelity Library Amplification Kits (Roche) in 20 µL reactions with 4µL Nextera Unique Dual Indexes Set A (Illumina) ...
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bioRxiv - Cell Biology 2023Quote: ... or Complete His-Tag Purification Resin (Roche, Basel, Switzerland), that had been prewashed with respective lysis buffer ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The plasmid constructs were transfected into HEK293 cells by using X-tremeGENE HP (Roche), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... N-Glycosidase F (Roche, #11365185001) was diluted 1:10 in serum-free IMDM and incubated with the cells for two hours at 37°C prior to fixation ...
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bioRxiv - Cell Biology 2023Quote: ... N-Glycans were enzymatically released by peptide N-glycosidase F (E.C. 3.5.1.52; Roche Applied Science) digestion then purified by C18-Sep-Pak (Waters Corp. ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cells were transiently transfected with C5aR2-Venus and βarr2-Rluc8 constructs using XTG9 (Roche). 24 hours post transfection ...
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bioRxiv - Cell Biology 2021Quote: Clarified supernatants were rocked with Complete His-Tag resin (Roche), 30 min ...
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bioRxiv - Microbiology 2020Quote: ... Clarified lysates were loaded onto His-tag purification column (Roche) and the protein was eluted in a buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... and incubated with 4mL cOmplete His-Tag Purification Resin (Roche) for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... 2 ml of the cOmplete His-Tag purification resin (Roche) equilibrated with the Tris buffer 4 containing 25 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and purified using cOmplete His-Tag Purification Resin (Roche, Switzerland) and His-Bind Purification kit (Novagen ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were arrested at metaphase by incubating with colcemid (N-methyl-N-deacetyl-colchicine, Roche, 10295892001) for the last 14 hours before harvesting the cells ...