Labshake search
Citations for Roche :
1 - 50 of 1765 citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2020Quote: ... 28 ng/μL fish sperm DNA (Roche) and 3 mM TCEP) ...
-
bioRxiv - Biophysics 2021Quote: ... 1 mg/ml fish sperm DNA (Roche) and 3 mM NaN3 (27) ...
-
bioRxiv - Molecular Biology 2019Quote: ... 50 µg boiled fish sperm carrier DNA (Roche 11467140001) was added to both linear and circular transformation reactions ...
-
bioRxiv - Plant Biology 2020Quote: ... and 0.5 μg double strand fish sperm DNA (Roche, USA). Cold competitor probe was used as 125-fold molar excess or as indicated elsewhere ...
-
bioRxiv - Cell Biology 2020Quote: ... 1% SDS (w/v) and 200 µg/ml fish sperm DNA solution (Roche). The 32P-labeled oligonucleotide probe (LD2648 (ITS1) ...
-
bioRxiv - Physiology 2023Quote: ... 0.2 mg/mL fish sperm ssDNA (Roche, 11467140001), 5x saline-sodium citrate (SSC ...
-
bioRxiv - Molecular Biology 2019Quote: ... DNA was added to a 50 µL aliquot of cells in addition to 50 µg boiled fish sperm carrier DNA (Roche 11467140001). Cells were equilibrated at 30° for 10 minutes after which 0.5 mL 40% Polyethylene glycol (PEG4000 ...
-
bioRxiv - Genomics 2023Quote: ... 1 M sodium phosphate (pH 7.2) and 0.5 ml of sonicated fish sperm solution (Roche Diagnostics, Switzerland). Probes were labeled with 32P by nick translation (New England Biolabs ...
-
bioRxiv - Genetics 2022Quote: ... Sperm DNA was extracted using the High Pure PCR Template Preparation Kit (Roche) modifying the first step of the protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... The membrane was incubated with labeled probe and 250 µg/mL salmon sperm DNA (Roche) at 65°C overnight in Perfecthyb Plus Hybridization Buffer (Millipore) ...
-
bioRxiv - Genomics 2022Quote: ... The labeled DNA fragment was ethanol-precipitated with sonicated salmon sperm DNA (Cat# D7656, Merk) and Escherichia coli tRNA (Cat# 10109541001, Roche Applied Science), and mouse Cot-1 DNA (Cat# 18440016 ...
-
bioRxiv - Developmental Biology 2022Quote: ... 5x SSC, 0.05mg/ml Heparin, 0.25% Tween20, 1% SDS, 1 mg/ml Salmon Sperm DNA, 1 mg/ml Roche blocking buffer powder) containing oligo probes at a concentration of 1μg/120μl and hybridized for two days at 43°C ...
-
bioRxiv - Cell Biology 2022Quote: ... or the fluorescein labeling kit for FISH (Roche). Probe for cmyb and WISH protocols have been previously described84–86.
-
bioRxiv - Developmental Biology 2020Quote: ... or for FISH (Roche anti-DIG-POD 1:1000). NBT/BCIP developed animals were stored in 80% glycerol overnight prior to mounting ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5X SSC, 5X Denhardt’s solution, 200 μg/ml yeast RNA, 500 μg/ml salmon sperm DNA and 2% Roche blocking reagent in DEPC treated water). Incubation with pre-hybridization buffer was carried out during 4h at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... 50% formamide, 5X SSC, 5X Denhardt’s, 200 μg/ml yeast RNA, 500 μg/ml salmon sperm DNA and 2% Roche blocking reagent in DEPC treated water) containing biotin and/or digoxin labeled Locked Nucleic Acid (LNATM ...
-
bioRxiv - Developmental Biology 2021Quote: ... Primary and secondary antibody for FISH were sheep anti-digoxigenin (Roche 11333089001) 1:375 ...
-
bioRxiv - Immunology 2019Quote: ... RNA FISH signal was detected by incubating FITC-labeled anti-digoxygenin antibody (Roche) in 4× SSC ...
-
bioRxiv - Microbiology 2020Quote: Biotinylated HIV-1 FISH probes were prepared with the Nick Translation Kit (Roche, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2019Quote: FISH probes were prepared from bacterial artificial chromosomes (BACs) using nick/translation (Roche 11745808910). 1ug BAC DNA was used in each 20uL nick/translation reaction along with the following fluorophores ...
-
bioRxiv - Immunology 2021Quote: ... slices were blocked for 30 min (2% BSA, 0.1ug/ul Salmon Sperm, 0.5% Saponin, 1 U/µl protector RNase inhibitor (Roche) in 3X SSC ...
-
bioRxiv - Genetics 2023Quote: ... Half of the sperm were incubated with 1X protease inhibitor cocktail (mini Complete EDTA-free tablet, Roche Diagnostic), incubated for 15 min on ice and sonicated ...
-
bioRxiv - Developmental Biology 2021Quote: ... 30-50 ventricles per group from fish of mixed sexes were dissociated using Liberase DH (Roche) and EGFP+ epicardial cells were isolated by FACS as described previously (Cao et al. ...
-
bioRxiv - Molecular Biology 2021Quote: ... Digoxigenin FISH signals were amplified using sheep anti-digoxigenin fluorescein fab fragments (1:50, Roche 11207741910) and fluorescein rabbit anti-sheep antibodies (1:100 ...
-
bioRxiv - Genomics 2022Quote: ... 3 mM MgCl2 and 2 mM CaCl2) containing 0.3 M of sucrose and MNase (30 units of MNase for every 2 million sperm, Roche Nuclease S7) was added to each tube ...
-
bioRxiv - Molecular Biology 2024Quote: ... beads were collected using a magnet stand at 4 °C and washed 3 times with beads wash buffer (sperm dilution buffer supplemented 1x cOmplete EDTA-free protease inhibitor cocktail (Roche, # 4693132001), 1x PhosSTOP (Roche ...
-
bioRxiv - Genetics 2023Quote: ... ssRNA FISH probes were labelled using FITC-UTP and purified through Mini Quick Spin RNA columns (Roche, #11814427001). Labelled double-stranded and ssRNA FISH probes were then precipitated in presence of 0.3M sodium acetate (Sigma ...
-
bioRxiv - Physiology 2022Quote: ... the linear segment relating the fish’s CoM to the center of the stimulus and b) the linear segment relating the fish’s CoM to its snout (Jornod and Roche 2015). These measures (4 and 5 ...
-
bioRxiv - Physiology 2021Quote: ... fish were taken out of their chamber one by one for a 2-min chase protocol (Roche et al., 2013) and returned in their chamber for immediate measurement of to estimate their maximum metabolic rate MMR (Fig ...
-
bioRxiv - Cancer Biology 2021Quote: ... Native fluorescence in situ hybridization (FISH) was performed by incubating cells with a hybridization mix (70% formamide, 1mg/mL Roche FISH blocking reagent 11096176001 ...
-
bioRxiv - Developmental Biology 2021Quote: ... FISH signal was developed with tyramide reaction following O/N incubation of embryos in sheep anti-DIG antibody (Roche; Cat#11222089001) (1:1000 ...
-
bioRxiv - Developmental Biology 2019Quote: ... Fluorescent in situ hybridisation (FISH) was carried out using either Fast Red tablets according to manufacturer’s instructions (Roche, discontinued from manufacturing) or Fast Blue BB Salt (Sigma ...
-
bioRxiv - Molecular Biology 2021Quote: ... the probe for RNA FISH was prepared with 2 µg of BAC RP183A17 using Nick translation kit (10976776001) purchased from Roche (Basel, Switzerland). Salmon sperm DNA and cot1 DNA were added to the probe to mask the repetitive sequence ...
-
bioRxiv - Developmental Biology 2021Quote: Immunostaining was performed after FISH development by re-blocking planarians in Blocking Solution (5% heat inactivated horse serum, 5% Roche Western Blocking Buffer in TNTx) for 2 hours at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... DNA fragments targeting the clpV gene were amplified from genomic DNA using KAPA HiFi DNA polymerase (Roche). Primers were designed from conserved regions identified by comparison of the clpV gene from the available K ...
-
bioRxiv - Genetics 2019Quote: ... DNA labelling was performed using random prime DNA labeling kit (Roche), and [α-32P] dCTP (PerkinElmer) ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1X Rapid DNA Ligation buffer (Rapid DNA Ligation Kit, Roche 11635379001), 1X Dilution buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with DNA using Xtreme-GENE 9 DNA (Roche) or with TransIT-HeLaMonster (Mirus ...
-
bioRxiv - Genomics 2024Quote: ... DNA was extracted using the MagNA Pure DNA isolation kit (Roche Molecular Systems ...
-
bioRxiv - Developmental Biology 2021Quote: ... DNA transfection was performed using X-tremeGENETM 9 DNA transfection reagent (Roche) in OptiMEM media according to the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2021Quote: ... 50ul of eluted digested DNA was ligated using T4 DNA ligase (Roche) in a volume of 2ml ...
-
bioRxiv - Microbiology 2020Quote: ... DNA amplifications were carried out with FastStart Essential DNA Probes Master (Roche), using specific primers and probes to Wolbachia pipientis WD0513 and Ae ...
-
bioRxiv - Microbiology 2022Quote: ... Total DNA was extracted with the MagNA Pure DNA isolation kit (Roche Molecular Systems ...
-
bioRxiv - Microbiology 2024Quote: ... and DNA ligations were performed using the Rapid DNA Ligation kit (Roche).
-
bioRxiv - Cancer Biology 2024Quote: ... DNA was extracted using the High Pure FFPET DNA Isolation Kit (Roche). Extracted FFPE DNA was repaired using the NEBNext FFPE DNA Repair Mix ...
-
Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... T4 DNA ligase (Roche) was used ...
-
bioRxiv - Genomics 2023Quote: ... Human genomic DNA (Roche) was amplified ...
-
bioRxiv - Developmental Biology 2024Quote: ... T7 DNA ligase (Roche) 25ng of backbone vector ...
-
bioRxiv - Cell Biology 2021Quote: ... BAC DNA was labeled with dUTP-bio (DNA-Synthesis) or dUTP-dig (Roche) by nick translation (Knoll and Lichter 2005 ...
-
bioRxiv - Cancer Biology 2022Quote: ... DNA was PCR amplified with FastStart Taq DNA Polymerase (Roche Diagnostics, Mannheim, Germany) using GeneAmp 9700 PCR system (Applied Biosystems ...