Labshake search
Citations for Roche :
1 - 50 of 1233 citations for Dengue Virus Subtype 4 Envelope 45 kDa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2019Quote: Dengue virus was quantified via RT-qPCR using the LightCycler 480 (Roche). We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... with virus packaging and envelope expressing plasmids using Fugene-6 (Roche). Medium was replaced with DMEM with 10% FCS the next day and supernatant was harvested 3 days post-transfection ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 0.4 µg vsFULL envelope plasmid using Xtremegene-9 (Roche). After 16 hours ...
-
bioRxiv - Microbiology 2022Quote: ... influenza virus hemagglutinin (Roche), followed by 1:4000 anti-rat horseradish peroxidase (HRP ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... 45 cycles on LC480 (Roche) - 94°C for 10 seconds ...
-
bioRxiv - Immunology 2019Quote: ... diluted 1:150 in washing buffer (45 min) and 4’,6-diamidino-2-phenylindole counterstain (DAPI; Roche/Sigma-Aldrich) diluted 1:250 in TBS (15 min ...
-
bioRxiv - Immunology 2022Quote: ... DNase I (45 μg/mL, Roche). The subsequent suspension was further dissociated using a GentleMACS dissociator (Miltenyi ...
-
bioRxiv - Immunology 2019Quote: ... DNase I (45 µg/mL, Roche). The subsequent suspension was further dissociated using a GentleMACS dissociator (Miltenyi) ...
-
bioRxiv - Genetics 2024Quote: ... and viral envelope vector gag-pol using XtremeGene 9 transfection reagent (Roche). Collection of viral particles and transduction was performed as described for lentiviral particles ...
-
bioRxiv - Molecular Biology 2023Quote: ... Alkaline phosphatase activity was detected in the dark in AP buffer supplemented with 45 mg/ml 4-nitrobluetetrazolium chloride (NBT, Roche) and 35 mg/ml 5-bromo-4-chloro-3-indolyl-phosphate (BCIP ...
-
bioRxiv - Cancer Biology 2021Quote: ... at 4 °C overnight and protein G-Agarose (Roche) at 4 °C for 3 h ...
-
bioRxiv - Genetics 2024Quote: ... and lentiviral envelope vector CMV VSV-G using XtremeGene 9 transfection reagent (Roche). Viral particles were collected 48h after transfection and passed through a 0.45 μm filter to eliminate cells and cell debris ...
-
bioRxiv - Cancer Biology 2023Quote: ... 45 U/mL creatine phosphokinase (Roche 10127566001), 10 mM HEPES pH 7.5 ...
-
bioRxiv - Microbiology 2021Quote: ... or LightCycler Multiplex RNA Virus Master (Roche). SYBR green based RT-qPCRs were performed using Luna Universal One-Step RT-qPCR Kit (NEB).
-
bioRxiv - Cell Biology 2021Quote: ... 45 ng/μl Proteinase K (Roche, Basel, Switzerland) was added followed by a second incubation step at 37°C for 30 min ...
-
bioRxiv - Molecular Biology 2019Quote: ... avian myeloblastosis virus reverse transcriptase (AMV RT; Roche) were used to synthesize cDNA from total RNA (2.5 µg ...
-
bioRxiv - Microbiology 2020Quote: ... or LightCycler® Multiplex RNA Virus Master (Roche) using CFX96 Real-Time System ...
-
bioRxiv - Microbiology 2022Quote: ... or LightCycler® Multiplex RNA Virus Master (Roche) using the CFX96 Real-Time System ...
-
bioRxiv - Microbiology 2020Quote: ... The Realtime Ready RNA Virus Master (Roche, Cat # 05619416001) was used to amplify viral RNA with the following primers/probe ...
-
bioRxiv - Neuroscience 2020Quote: ... 45 μl of Fugene HD transfection reagent (Roche, Laval, QC) was diluted in 400 μl of OPTI-MEM ...
-
bioRxiv - Microbiology 2020Quote: ... For IVD approved LightCycler® Multiplex RNA Virus Master (Roche) 5 μl of template RNA were used ...
-
bioRxiv - Immunology 2021Quote: ... and calf thymus histones (45 mg/Kg in sterile saline; Roche® distributed by Sigma-Millipore ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... or p89.6) and an envelope deficient HIV-1 backbone vector (pSG3Δenv) using XtremeGENE HP DNA Transfection Reagent (Roche). After 24 hours of incubation at 37°C and 5% CO2 ...
-
bioRxiv - Biochemistry 2023Quote: ... pCW57.1_yeastAOX 32 with the Delta-Vpr packaging plasmids and the VSV-G envelope plasmid (DNA concentration ratio, 1.2: 1: 0.3) into HEK293T cells using X-tremeGENE 9 Transfection Reagent (Roche). Lentivirus-containing media was harvested at 24 hr after fresh media change ...
-
bioRxiv - Cell Biology 2023Quote: ... Regions of interest were cut from the nitrocellulose membrane (70 kDa to 130 kDa) and RNA was released from the membrane using Proteinase K (Roche, 03115828001). RNA was purified using neutral phenol/chloroform/isoamylalcohol (Ambion ...
-
bioRxiv - Cancer Biology 2023Quote: Lentivirus was generated by co-transfecting HEK293T cells with the lentiviral construct pCMV-dR8.91 (Δ8.9) plasmid and the pMDG envelope-expressing plasmid using X-tremeGENE 9 DNA Transfection Reagent (Roche, 6365779001). Viral supernatant was collected within 24-48 h post-transfection for subsequent infection of the target cells.
-
bioRxiv - Microbiology 2022Quote: ... For the IVD-approved LightCycler® Multiplex RNA Virus Master (Roche), 5 µL of template RNA in a total reaction volume of 20 µL was used ...
-
bioRxiv - Biochemistry 2023Quote: ... Virus passages were then amplified in penicillin-streptomycin-containing media (Roche) until cells became visibly swollen and demonstrated reduced viability statistics ...
-
bioRxiv - Systems Biology 2019Quote: ... along with the packing plasmid psPAX2 (16.2 μg) and envelope plasmid pMD2.G (1.8 μg) were transfected using Fugene 6 (Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... pUMVC for retrovirus) and 250 ng envelope vector (pCMV-VSV-G) are mixed with 20 µL Fugene 6 (Roche, USA) diluted in OptiMEM ...
-
bioRxiv - Genomics 2024Quote: ... Cells were then transfected with the Tet-pLKO-puro plasmids targeting either UHRF1 or DNMT1 transcripts and the accompanying lentiviral packaging (psPAX2) and envelope (pMD.2G) plasmids with Opti-MEM and Xtreme HP Gene transfection reagent (Roche) per the manufacturer’s protocol ...
-
bioRxiv - Zoology 2020Quote: ... buds were incubated 45 mins in a blocking solution (Blocking Reagent (BR) (Roche) at 2% and saponine (Sigma ...
-
bioRxiv - Microbiology 2019Quote: ... 9016-45-9]) supplemented with complete protease inhibitor cocktail (cOmplete, EDTA-free [Roche]). Cell lysates were passed 3 times through a 25 G needle ...
-
bioRxiv - Microbiology 2019Quote: ... 9016-45-9]) supplemented with complete protease inhibitor cocktail (cOmplete, EDTA-free [Roche]). Cell lysate was passed 3 times through a 25 G needle ...
-
bioRxiv - Microbiology 2022Quote: ... 9016-45-9]) supplemented with complete protease inhibitor cocktail (cOmplete, EDTA free; Roche) and phosphatase inhibitor (PhosSTOP ...
-
bioRxiv - Microbiology 2022Quote: ... using the LightCycler Multiplex DNA Virus Master kit (Roche Diagnostics, Mannheim, Germany) on a LightCycler 480 instrument (Roche Diagnostics).
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... including 5 μL template using LightCycler Multiplex RNA Virus Master (Roche, Switzerland). All assays were carried out in triplicate on the Roche Lightcycler 96 platform (Roche ...
-
bioRxiv - Cancer Biology 2023Quote: ... Virus was produced in 293T cells transfected using X-tremeGENE HP (Roche) with viral constructs along with VSV-G and gag-pol ...
-
bioRxiv - Immunology 2022Quote: ... treated for 45 min at 37 °C with collagenase D (Roche, 200 μg/ml), and prepared into a cell suspension ...
-
bioRxiv - Immunology 2021Quote: ... treated for 45 min at 37°C with collagenase D (Roche, 100 ng/ml), and a cell suspension was prepared ...
-
bioRxiv - Immunology 2021Quote: ... treated for 45 min at 37 °C with collagenase D (Roche, 200 μg/ml), and prepared into a cell suspension ...
-
bioRxiv - Plant Biology 2024Quote: ... SeqCap EZ HyperCap exome capture [45] was performed as per the manufacturer’s instructions (Roche Sequencing Solutions ...
-
bioRxiv - Cell Biology 2021Quote: ... and mouse/rabbit IgG overnight at 4°C and further with protein G-coupled agarose beads (ROCHE) for 1-2 h ...
-
bioRxiv - Cell Biology 2020Quote: ... minced and enzymatically dissociated at 37°C for 45 min in 0.2% collagenase A (Roche) and 1 mg/ml dispase (GibcoBRL) ...
-
bioRxiv - Neuroscience 2023Quote: ... Plates were run on a 45-cycle protocol using the LC 480 II system (Roche).
-
bioRxiv - Cell Biology 2021Quote: ... The virus was pretreated with 20 U ml-1 RNase-free DNase I (Roche) for 1 h at 37°C ...
-
bioRxiv - Microbiology 2020Quote: ... DENV-3 RNA was amplified by qRT-PCR (LightCycler Multiplex RNA Virus Master, Roche), using primers to the conserved 3’UTR ...
-
bioRxiv - Microbiology 2021Quote: RNA from virus was extracted using High Pure Viral Nucleic acid extraction kit (Roche). Using gene specific reverse primers of target genes ...
-
bioRxiv - Microbiology 2022Quote: ... Virus RNA was purified using the High Pure Viral RNA kit (Roche, Basel, Switzerland), amplified using universal eight-segment primers and directly sequenced ...
-
bioRxiv - Microbiology 2023Quote: ... DENV-2 RNA was amplified by qRT-PCR (LightCycler Multiplex RNA Virus Master, Roche), using primers to the conserved 3’UTR ...