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Citations for Roche :
1 - 50 of 6629 citations for Dengue Virus NS1 Protein Serotypes 1 4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2019Quote: Dengue virus was quantified via RT-qPCR using the LightCycler 480 (Roche). We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2022Quote: ... labeling probe (359 bp) was synthesized using primers TiPV-Fq and TiPV-NS1-R (Table 1) and DIG-labeling mix reagent (Roche, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... influenza virus hemagglutinin (Roche), followed by 1:4000 anti-rat horseradish peroxidase (HRP ...
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bioRxiv - Cancer Biology 2021Quote: ... at 4 °C overnight and protein G-Agarose (Roche) at 4 °C for 3 h ...
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bioRxiv - Cell Biology 2021Quote: ... The virus was pretreated with 20 U ml-1 RNase-free DNase I (Roche) for 1 h at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... or LightCycler Multiplex RNA Virus Master (Roche). SYBR green based RT-qPCRs were performed using Luna Universal One-Step RT-qPCR Kit (NEB).
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bioRxiv - Molecular Biology 2019Quote: ... avian myeloblastosis virus reverse transcriptase (AMV RT; Roche) were used to synthesize cDNA from total RNA (2.5 µg ...
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bioRxiv - Microbiology 2020Quote: ... or LightCycler® Multiplex RNA Virus Master (Roche) using CFX96 Real-Time System ...
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bioRxiv - Microbiology 2022Quote: ... or LightCycler® Multiplex RNA Virus Master (Roche) using the CFX96 Real-Time System ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-Green Fluorescent Protein overnight at 4°C (Clones 7.1 and 13.1; 1:50 dilution, Roche, Mannheim, Germany) and rat monoclonal anti-HA High Affinity for 60 minutes at room temperature (clone 3F10 ...
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bioRxiv - Microbiology 2020Quote: ... The Realtime Ready RNA Virus Master (Roche, Cat # 05619416001) was used to amplify viral RNA with the following primers/probe ...
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bioRxiv - Microbiology 2020Quote: ... For IVD approved LightCycler® Multiplex RNA Virus Master (Roche) 5 μl of template RNA were used ...
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bioRxiv - Biochemistry 2020Quote: ... the expression medium containing the secreted fusion protein was supplemented with 1/4 tablet of protease inhibitor (complete EDTA-free protease inhibitor cocktail tablets, Roche Diagnostic GmbH, 45148300) and transferred to a 15 ml Falcon tube containing 200 μl of washed ...
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bioRxiv - Neuroscience 2021Quote: ... or precleared for 1 hr using 30 μl dry volume of a 1:1 mixture of protein-A and protein-G agarose (Roche Diagnostics). Solubilized membranes (3 mg of total membrane protein ...
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bioRxiv - Microbiology 2022Quote: ... For the IVD-approved LightCycler® Multiplex RNA Virus Master (Roche), 5 µL of template RNA in a total reaction volume of 20 µL was used ...
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bioRxiv - Cell Biology 2023Quote: ... with virus packaging and envelope expressing plasmids using Fugene-6 (Roche). Medium was replaced with DMEM with 10% FCS the next day and supernatant was harvested 3 days post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Virus passages were then amplified in penicillin-streptomycin-containing media (Roche) until cells became visibly swollen and demonstrated reduced viability statistics ...
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bioRxiv - Microbiology 2022Quote: ... using the LightCycler Multiplex DNA Virus Master kit (Roche Diagnostics, Mannheim, Germany) on a LightCycler 480 instrument (Roche Diagnostics).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... including 5 μL template using LightCycler Multiplex RNA Virus Master (Roche, Switzerland). All assays were carried out in triplicate on the Roche Lightcycler 96 platform (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus was produced in 293T cells transfected using X-tremeGENE HP (Roche) with viral constructs along with VSV-G and gag-pol ...
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bioRxiv - Neuroscience 2020Quote: ... levodopa (Madopar®, Roche, Levodopa/carbidopa, ratio 4:1) was administered twice daily for 4-5 months at an individually-tailored dose designed to produce a full reversal of the parkinsonian condition (p.o ...
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bioRxiv - Pathology 2022Quote: RNA was reverse-transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (LifeTechnologies, Carlsbad, CA) on the LightCycler 480 or LC96 instrument (Roche, Indianapolis, IN), and quantified by interpolation onto a standard curve made up of serial tenfold dilutions of in vitro transcribed RNA ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse/rabbit IgG overnight at 4°C and further with protein G-coupled agarose beads (ROCHE) for 1-2 h ...
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bioRxiv - Developmental Biology 2019Quote: ... 1/10 volume of Protein-G agarose beads (Roche) was then added and the sample rotated for 30 min at RT °C ...
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bioRxiv - Microbiology 2020Quote: ... DENV-3 RNA was amplified by qRT-PCR (LightCycler Multiplex RNA Virus Master, Roche), using primers to the conserved 3’UTR ...
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bioRxiv - Microbiology 2021Quote: RNA from virus was extracted using High Pure Viral Nucleic acid extraction kit (Roche). Using gene specific reverse primers of target genes ...
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bioRxiv - Microbiology 2022Quote: ... Virus RNA was purified using the High Pure Viral RNA kit (Roche, Basel, Switzerland), amplified using universal eight-segment primers and directly sequenced ...
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bioRxiv - Microbiology 2023Quote: ... DENV-2 RNA was amplified by qRT-PCR (LightCycler Multiplex RNA Virus Master, Roche), using primers to the conserved 3’UTR ...
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bioRxiv - Physiology 2020Quote: ... Proteins were extracted using 1:1 Pierce RIPA solution with protease inhibitor tablet (Roche). Samples were vortexed for 5 seconds ...
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bioRxiv - Neuroscience 2020Quote: ... at 4°C with alkaline phosphate anti-DIG (1:2000, Roche). For chromogenic revelation ...
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bioRxiv - Plant Biology 2023Quote: ... 1/200 mM NaF) containing 4 tablets of protease inhibitor (Roche cOmplete ...
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bioRxiv - Immunology 2024Quote: ... beads and lysis buffer (20 mM Tris pH 7.4, 120 mM NaCl, 1 mM EDTA, 1% Triton-X-100, 0.5% sodium deoxycholate, 1× protease inhibitor cocktail [Roche])) ...
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bioRxiv - Microbiology 2020Quote: Quantification of the virus by qPCR was performed using the LightCycler 480 System (Roche, France) and specific primers designed in the non-structural gene NS1 (qAalDV2-F ...
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bioRxiv - Biochemistry 2022Quote: Proteins were extracted with 1× RIPA buffer containing 1× complete-mini protease inhibitor (Roche: 11836170001) and 1× phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Biophysics 2021Quote: ... transient transfection was performed with a total of 1 µg of plasmids (EGFRmCitrine, PTBmCherry and cCblBF P at ratio 4:3:4 by mass) using FUGENE6 (Roche Diagnostics) transfection reagent and Opti-MEM (Gibco - Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants were diluted 1:4 with ice-cold dilution buffer (1× phos-stop [Roche], 0.5% NP-40, 1 mM DTT) and centrifuged again at 15,000 rpm for 15 minutes at 4°C to precipitate actomyosin components ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified for 30min at 15,000xg and the supernatant was immunoprecipitated for 2h at 4°C with Dynabeads-protein G covalently coupled to the rat anti-HA antibody (Roche). The beads were washed 3×5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Then cells were lysed in B-per protein extraction reagent 4 ml/ gram bacterial pellet in the presence of cOMPLETE mini protease inhibitor cocktail (Roche) for 15 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... the lysates were centrifuged (10 min, 20000 g, 4 °C) and the resulting supernatants were added to 25 µl Protein G Agarose (Roche), rotating for 1 hour at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... incubation at 4 °C rotating for 3 h, followed by incubation (4 °C, rotating) with 30 μl of pre-washed Protein A agarose bead slurry (#11134515001, Roche) for an additional hour ...
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bioRxiv - Microbiology 2019Quote: ... Protein extracts were incubated for 3 h on a spinning wheel at 4°C with 40 µl of Protein G Sepharose beads (Roche) and 2.5 µg of the specific BTV-NS3 antibody ...
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bioRxiv - Plant Biology 2023Quote: ... Meristem-enriched samples or whole seedlings were ground in liquid nitrogen and homogenized in cold protein extraction buffer (50 mM Tris HCl pH 7.5, 10% glycerol, 1 mM DTT, 1% IGEPAL, 1× Roche EDTA-free protease inhibitor and 1× Roche phosphatase inhibitor) ...
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bioRxiv - Immunology 2022Quote: ... RT-PCR was performed using the LightCycler Multiplex RNA Virus Master Kit (Roche Diagnostics, Mannheim, Germany) on a Cobas z480 analyzer (Roche Diagnostics ...
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bioRxiv - Immunology 2021Quote: ... Cellular mRNA of cells not exposed to virus was isolated with an mRNA Capture kit (Roche) and cDNA was synthesized with a reverse-transcriptase kit (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... Protein translation was induced with 1 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG, 11411446001, Roche Diagnostics) and cultures were incubated overnight at 18 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... a 4-minute digestion with proteinase K (Roche, 1:1000 in PBS-DEPC) is followed by probe titration (100-300 ng per slide dependent on the probe ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then blocked overnight at 4°C in 1 % blocking reagent (Roche) plus 5 % sheep serum in MAB and incubated with anti-DIG conjugated to Peroxidase (1/50 ...
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bioRxiv - Cell Biology 2023Quote: ... fibroblasts were fixed with PFA 4 % and stained with DAPI (Roche; 1:10,000) for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... HA-tagged proteins were detected using 1:1,000 anti-HA antibody (Roche) and 1:5,000 anti-rat antibody (Abcam) ...
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bioRxiv - Biochemistry 2022Quote: ... The relipidated proteins were treated with 1 mM AMPPNP (sodium salt, Roche) for overnight at 4 °C and desalted with a PD-10 column ...