Labshake search
Citations for Roche :
1 - 50 of 2312 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2019Quote: ... ~2 x 108 parasites were resuspended in 2 mL cytoplasmic lysis buffer (10 mM HEPES [pH 8], 10 mM KCl, 0.1 mM EDTA [pH 8], plus complete protease inhibitor [PI, Roche # 11836170001]), transferred to a prechilled 2 mL douncer homogenizer and set on ice for 30 min ...
-
bioRxiv - Plant Biology 2020Quote: ... 10 mM ascorbic acid) containing a protease inhibitor cocktail (Roche). All subsequent steps were conducted on ice ...
-
bioRxiv - Molecular Biology 2020Quote: ... and then deproteinized by adding of 8 µl of 10% SDS and 8 µl of proteinase K (19 mg/ml stock, Roche). Deproteinization was carried out at 37 °C for 30 min with gentle flicking ...
-
bioRxiv - Biochemistry 2021Quote: ... 0.5% deoxycholic acid) containing inhibitors of phosphatases (1:10 PhosphoStop; Roche) and proteases (1:100 Protease Inhibitor Cocktail ...
-
bioRxiv - Cell Biology 2022Quote: ... each fatty acid was first conjugated to 10% fatty-acid-free BSA (bovine serum albumin fraction V) (#10735086001, Roche) for 1 hour at 50°C in a 50:50 volumetric ratio ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were resuspended in 450 μl lysis buffer (20 mM Tris pH 7.5, 0.5 mM EDTA pH 8, 200 mM NaCl, 10% glycerol, 1 mM PMSF, 10 mM NEM, Roche cOmplete protease inhibitor catalog # 11836153001) ...
-
Genomic organization of the autonomous regulatory domain of eyeless locus in Drosophila melanogasterbioRxiv - Genetics 2021Quote: ... The fixed embryos were resuspended in 2.5 ml of ice-cold lysis buffer (10 mM Tris-pH-8, 10 mM NaCl, 0.2% NP40 with Roche protease inhibitor cocktail freshly added ...
-
bioRxiv - Molecular Biology 2019Quote: ... Cell pellets were incubated in 1 ml ice-cold lysis buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.2% NP-40, protease inhibitor cocktail (Roche) for 15 min on ice ...
-
bioRxiv - Biochemistry 2019Quote: ... washed with PBS and suspended in Ni-wash buffer (20 mM Tris/HCl pH 8, 10 mM imidazole, 200 mM NaCl, 10 mM DTT, 2 mM PMSF, 0.5x Roche Protease Inhibitor cocktail ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were resuspended in cold lysis buffer (10 mM Tris pH 8, 10 mM NaCl, 0.2% NP-40, 1x protease inhibitors (Roche)) and incubated for 20 min on ice ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were lysed in IP buffer (10 mM Tris pH 8, 0.4% NP40, 300 mM NaCl, 10% glycerol, 1 mM DTT, antiprotease (Roche), anti-phosphatase (Sigma ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells were lysed in hypotonic lysis buffer (10 mM Tris/HCl pH 8, Roche protease inhibitors ...
-
bioRxiv - Microbiology 2024Quote: ... and 8 to 10 PCR cycles with the KAPA HiFi PCR kit (Kapa Biosystems) were used for amplification ...
-
bioRxiv - Microbiology 2022Quote: ... cells were recovered and lysed in 500 µl hypotonic lysis buffer (10 mM Tris-HCl pH 8, 10 mM KCl, 1× complete protease inhibitor cocktail (Roche)) ...
-
bioRxiv - Molecular Biology 2023Quote: ... in complementary deoxyribonucleic acid (cDNA) from 10 ng total RNA in 10 μl reactions with 2× Master Mix (Roche) in a StepOnePlus Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Genomics 2019Quote: ... and 8–10 kb) were constructed with the KAPA Hyper Prep Kit (Kapa Biosystems, Roche) and Nextera Mate Pair Library Preparation Kit ...
-
bioRxiv - Genomics 2019Quote: ... and 8–10 kb) were constructed with the KAPA Hyper Prep Kit (Kapa Biosystems, Roche) and Nextera Mate Pair Library Preparation Kit ...
-
bioRxiv - Genomics 2021Quote: ... The ligated products were enriched with 8-10 cycles of PCR (HiFi premix, KAPA biosystems) and size selected to 200-500 bp with AMPure XP beads (Agencourt) ...
-
bioRxiv - Cell Biology 2019Quote: ... with ATP standard solutions used at concentrations between 10−8 and 10−5 M (luciferin/luciferase ATP bioluminescence assay kit CLSII, Roche, Basel, Switzerland). Data are expressed as nmol ATP produced/min/106 cells.
-
bioRxiv - Developmental Biology 2021Quote: ... and PCR enriched (8-10 cycles) using the KAPA Hyper Library Preparation kit (KAPA Biosystems, #KK8504). eCLIP libraries were prepared as described above according to (Van Nostrand et al. ...
-
bioRxiv - Cancer Biology 2019Quote: ... 10 mM Tris-HCl pH 8 supplemented with phosphatase and protease inhibitor cocktail tablets (Roche Diagnostics)) ...
-
bioRxiv - Microbiology 2023Quote: ... pH 8) containing 10 mM Imidazole and supplemented with Protease Inhibitor Mixture (Complete, Roche Applied Science) for lysis at 4 °C by sonication ...
-
bioRxiv - Pathology 2022Quote: ... 10 mM Okadaic acid) containing protease inhibitor cocktail (cOmplete ULTRA Tablets Mini EASYpack; Roche Diagnostics). Protein concentration was measured by BCA Protein Assay Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... 10 mM Okadaic acid) containing protease inhibitor cocktail (cOmplete ULTRA Tablets Mini EASYpack; Roche Diagnostics). The protein concentration was measured using BCA Protein Assay Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 50 mM Tris pH 8) containing protease inhibitors (Roche mini EDTA-free, 1 tablet in 10 ml) and phosphatase inhibitors (2mM Sodium Ortho-Vanadate ...
-
bioRxiv - Microbiology 2022Quote: ... and lentivirus vectors at a 2:8:10 μg ratio to HEK293T cells using Xtreme-Gene9 (Roche) and lentivirus-containing supernatants were collected 72hr post-transfection ...
-
bioRxiv - Genetics 2020Quote: ... 0.1 M ammonium bicarbonate, pH 8, 150 mM NaCl, 1 Roche mini protease inhibitor tablet without EDTA/10 ml, ½ Roche phosSTOP tablet/10 ml) using 14 × 1.5 min bursts on a BioSpec mini bead-beater at room temperature ...
-
bioRxiv - Molecular Biology 2020Quote: ... 0.5% blocking buffer pH 7.5 (100 mM maleic acid, 150 mM NaCl, 10% Roche blocking reagent #11096176001). Antibody staining was performed in 3% BSA in PBS-Triton (0.1%) ...
-
bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma], 10 mM NaCl [Sigma], 0.2 % Nonidet P-40 substitute [Sigma], 1x Complete EDTA-free Protease inhibitor [Roche]) for 30 min at 4 °C ...
-
bioRxiv - Microbiology 2019Quote: ... beads containing bound methylated DNA were resuspended in 250 μl digestion buffer (50 mM Tris pH 8, 10 mM EDTA, 0.5% SDS) with 100 μg Proteinase K (Roche) and incubated at 65°C for 4 h ...
-
bioRxiv - Molecular Biology 2021Quote: ... washed in cold PBS and incubated on ice in cold swelling buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.2% NP-40, 1 mM AEBSF and 1x complete mini EDTA-free proteasome inhibitor, Roche) for 10 min ...
-
bioRxiv - Immunology 2021Quote: ... Pellets of cross-linked cells were resuspended in shearing buffer (10 mM Tris-HCl pH 8, 0.1% SDS, 1 mM EDTA) supplemented with Complete Protease Inhibitor Cocktail (Roche), and chromatin was sheared to an average fragment size of 200-250 bp ...
-
bioRxiv - Cell Biology 2021Quote: ... membranes were removed from neurons cultured in MatTek dishes at 8-10 days in vitro (DIV) by incubating cells with extraction buffer (1M sucrose, 0.15% Triton X-100 (Roche, Basel ...
-
bioRxiv - Molecular Biology 2020Quote: ... Released nuclei were washed (10 mM Tris-HCl pH 8, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1x PIC (Roche)) for 5 minutes at 4°C ...
-
bioRxiv - Genomics 2020Quote: ... Isolated nuclei were then lysed with lysis buffer (50mM Tris-HCl, pH 8.1, 10 mM EDTA, pH 8, 1% SDS, 1X protease inhibitor cOmplete EDTA-free cocktail, Roche). Nuclei were sonicated using a Bioruptor ultrasonic cell disruptor (Diagenode ...
-
bioRxiv - Immunology 2021Quote: ... Add 120 ul of fresh ChIP SDS Lysis Buffer (0.5% SDS, 50 mM Tris, pH 8, 10 mM EDTA, 1 × protease inhibitor cocktail (Roche)) per 5 million cells ...
-
bioRxiv - Plant Biology 2023Quote: ... in 100μl GTEN-buffer (10% Glycerol, 25mM TRIS pH 8, 0.5mM EDTA, 150 mM NaCl,1 x protease inhibitors (Roche), 5mM DTT ...
-
bioRxiv - Plant Biology 2023Quote: ... A GTEN-based buffer (10% Glycerol, 25mM TRIS pH 8, 0.5mM EDTA, 150 mM NaCl, 1 x protease inhibitors (Roche), 5mM DTT ...
-
bioRxiv - Biochemistry 2023Quote: ... washed with 1 x PBS and resuspended in 100 μl of SoTE buffer (10 mM Tris-HCl pH 8, 1 mM EDTA, 0.15 % Triton X-100, protease inhibitor cocktail (Roche)) ...
-
bioRxiv - Molecular Biology 2024Quote: Ni-NTA Magnetic Agarose Beads were incubated with 1.5µg of recombinant His-TIRR(8) in binding buffer (50mM Tris pH 7.4, 150mM NaCl, 5mM MgCl2, 10% Glycerol, Protease inhibitors (EDTA free, Roche)) for 1 hour at 4°C on a wheel ...
-
bioRxiv - Cell Biology 2021Quote: ... membranes were blocked with 10 mL of 1x blocking solution diluted in 1x Maleic Acid Buffer (Roche, 115857262001) with 0.3% TWEEN 20 for 30 minutes at room temperature ...
-
bioRxiv - Cancer Biology 2020Quote: ... Linoleic acid (C18) complexed with fatty acid free BSA (Roche 10775835001). PBS and BSA were used as the vehicle control in experiments containing C8 and C18 respectively ...
-
bioRxiv - Biochemistry 2019Quote: ... The cells were then pelleted again and resuspended in 150 μL of Lysozyme buffer (150 mM NaCl, 30 mM Tris–HCl pH 8, 10 mM EDTA, cOmplete protease inhibitor (Roche), 1 mg/ml lysozyme ...
-
bioRxiv - Biochemistry 2019Quote: ... The cells were then pelleted again and resuspended in 150 μL of Lysozyme buffer (150 mM NaCl, 30 mM Tris–HCl pH 8, 10 mM EDTA, 1 mM TCEP, cOmplete protease inhibitor (Roche), 1 mg/ml lysozyme ...
-
bioRxiv - Microbiology 2019Quote: ... The cells were washed twice with PBS and lysed with 6 ml of lysis buffer (8 M urea, 150 mM NaCl, 100 mM ammonium bicarbonate, pH 8; added per 10 ml of buffer: 1 tablet of Roche mini-complete protease inhibitor EDTA free and 1 tablet of Roche PhosSTOP tablet ...
-
bioRxiv - Genetics 2020Quote: ... and resuspended in 300 μL of Nuclei Lysis Buffer (Tris-HCl pH 8 50 mM, EDTA 10 mM, SDS 0.5% and protease inhibitor (Roche-04693132001). Nuclei were then sonicated (Diagenode bioruptor plus sonicatore ...
-
bioRxiv - Microbiology 2020Quote: ... pelleted and resuspended in 150 μl sonication buffer [(50 mM Tris pH 8, 1% SDS, 10 mM EDTA, 1x protease inhibitor (Roche)] ...
-
bioRxiv - Developmental Biology 2020Quote: ... Nuclei were pelleted by centrifugation and resuspended in sonication buffer (50 mM Tris-HCl pH 8, 1% SDS, 10 mM EDTA, 1x cOmplete ULTRA Tablet (Roche)) and sonicated using a Bioruptor sonicator (Diagenode ...
-
bioRxiv - Genetics 2019Quote: ... cells were vortexed and passed through a syringe in ubiquitin lysis buffer (2% SDS, 150mM NaCl, 10 mM Tris HCl pH 8, 1 Roche protease inhibitor tablet ...
-
bioRxiv - Cell Biology 2019Quote: ... washed in PBS, and resuspended in Lysis Buffer (Tris 10 mM pH 8, 2 mM EDTA) supplemented with Complete protease inhibitor (Roche, Merck ...