Labshake search
Citations for Roche :
1 - 50 of 7592 citations for 6β Fluoro 21 hydroxypregna 1 4 9 11 16 tetraene 3 20 dione 21 acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... MED26 probe: #21 (universal probe library, Roche). The nascent transcription of HSPA1A and HSPH1 was compared against nascent transcription of Mediator subunit 26 (MED26) ...
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bioRxiv - Genomics 2021Quote: ... 10 nm of Universal Probe #21 (Roche Diagnostics, Australia). Cycling conditions were 95 °C for 5 minutes ...
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bioRxiv - Genetics 2023Quote: ... with the Universal Probe Library #21 probe (Roche, Indianapolis, Indiana). qPCR was performed with one 95°C incubation for 5 minutes ...
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bioRxiv - Genomics 2021Quote: Probes 1-3 were labelled with digoxigenin-11-dUTP or biotin-11-dUTP (Roche) using BioPrime® Array CGH ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown in 10 μM BrdU:BrdC (3:1) for 16–20 h before incubation with 0.1 μg/mL colcemid (Roche) for 2-3 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Then a 21-cycle PCR was performed using KAPA HiFi HotStart Uracil+ ReadyMix (KAPA Biosystems) and universal primers ...
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bioRxiv - Developmental Biology 2019Quote: ... To increase probe permeability embryos were incubated at 21°C in 10µg/ml proteinase K (Roche) for the following durations ...
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bioRxiv - Neuroscience 2019Quote: ... BCIP (5-Bromo-4-chloro-3-indolyl-phosphate, 4-toluidene salt, Roche, Cat. No. 11 383 221 001. 105 µl/40 ml) and Levamisole (Vector ...
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bioRxiv - Genetics 2019Quote: ... 20 µM digoxigenin-11-UTP (Roche Diagnostics, Mannheim, Germany), 1× nick translation buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... and HH20/21 chick embryos were harvested and neuroectoderm was isolated after Dispase I (Cat No. 4942086001, Roche) treatment (Pearson et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... In vitro translation reactions with luciferase reporter mRNAs were carried out with 0.5x extract, energy mix (final 0.84 mM ATP, 0.21 mM GTP, 21 mM creatine phosphate (Roche 10621722001), 45 U/mL creatine phosphokinase (Roche 10127566001) ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were reverse transfected with 1:3 or 1:6 X-tremeGENE 9™ (Roche) transfection reagent according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... A ∼3:1 ratio of X-tremeGENE™ 9 DNA Transfection Reagent (Roche, XTG9-RO) was added to the mixture prior to incubation and application on HEK293T cells using standard methods 1 ...
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bioRxiv - Genomics 2020Quote: ... Probe DNA was nick-translated with either Digoxigenin-11-UTP or Biotin-16-UTP (Roche) using DNase I (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... with added Roche cOmplete protease inhibitor (# 11836170001, Roche. Samples were further diluted 21 times in 5x Laemmli loading buffer containing Roche protease inhibitor ...
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bioRxiv - Neuroscience 2020Quote: ... nt 33-140 from sequence NM_016967 detected with the mOLIG2 probe (Universal ProbeLibrary probe #21, Roche, Cat.No. 04686942001; 5′-(Fam)-CAGAGCCA-(Dark Quencher)-3′) ...
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bioRxiv - Cell Biology 2020Quote: ... and SD-N (0 and 24 h) samples were TCA precipitated [21] and analyzed by Western blot with anti-GFP bodies (11814460001; Roche). All the experiments were done at least in duplicate.
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bioRxiv - Cell Biology 2021Quote: ... whole testes from mice at postnatal day 21(P21) were lysed in RIPA buffer containing protease-inhibitor cocktail (11697498001, Roche). Protein lysates were then blocked by incubation with protein A Dynabeads (10002D ...
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bioRxiv - Bioengineering 2023Quote: ... SOS1•RAS complex was formed by incubating SOS1 and RAS at a stochiometric ratio of 1:3 overnight at 4° with 20 mM EDTA and alkaline phosphatase (Roche). The complex was then purified by gel filtration ...
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bioRxiv - Neuroscience 2021Quote: ... and Biotin-16-dUTP (Roche Diagnostics, 4 μm) and added to slides for 1 h at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Beads containing cDNA were then eluted and cDNA was amplified for 21 cycles using Kapa HiFi Hot start ready mix (Roche #07958935001). Amplified cDNA was then purified (GC biotech CPCR-0050 ...
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bioRxiv - Immunology 2021Quote: ... 3 × 104 HMEC-1 cells were seeded into an E-16 multi-well plate (Roche) in triplicate and incubated for 72 h ...
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bioRxiv - Developmental Biology 2022Quote: ... DNA probes were labelled by nick translation with either biotin-16-dUTP or digoxigenin-11-dUTP (Roche). For simultaneous IF and RNA FISH ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The labelling nucleotide mix contained either biotin-16-dUTP or digoxigenin-11-dUTP (both Roche, Mannheim, Germany). Due to its long size ...
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bioRxiv - Microbiology 2019Quote: ... the ORF50 trans-complementing BHK-21 cells were transfected with 2 μg of ΔORF50 BAC DNA using FuGENE HD Transfection Reagent (Roche, Mannheim, Germany). When cells showed a visible CPE ...
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bioRxiv - Molecular Biology 2023Quote: ... Co-purification of FlgP or PflD-sfGFP was investigated by western blot (WB) analysis using FlgP specific antisera (21) or an anti-GFP antibody (Roche #11814460001, RRID:AB_390913), respectively ...
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bioRxiv - Genomics 2020Quote: ... and a potential large inversion on chromosome 4 (Supplementary Table 11) were labeled with digoxigenin-11-dUTP (Roche, Indianapolis, IN), biotin-16-dUTP (Roche) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Rap1GAP- RlucII/rGFP-CAAX + Gαi2 and D2 or PDZ-RhoGEF-RlucII/rGFP-CAAX + Gα13 and TPαR) using X-tremeGENE 9 DNA transfection reagent (3:1 reagent/DNA ratio; Roche) diluted in OptiMEM (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... PFN1-KO or PFN2-KO) were split 1:3 and the next day transfected using XtremeGene 9 (Roche) with 5 μg V5-NAA80 (M23L ...
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bioRxiv - Genomics 2022Quote: ... Cycle Pure Kit (Omega) and then labelled with either biotin–16-dUTP or digoxigenin–11-dUTP (Roche Diagnostics) using the BioPrime Array CGH random priming kit (Invitrogen) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1:3000 (Roche, 11 093 274 910), and the alkaline phosphatase substrate BM purpled (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 1000 µL cold cytoplasmic lysis buffer (10 mM HEPES pH 7.9, 10 mM KCl, 2 mM Mg acetate, 3 mM CaCl2, 340 mM sucrose, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA to 1× ...
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bioRxiv - Microbiology 2023Quote: ... cells were resuspended in 1000 µL cold sucrose buffer containing (10 mM HEPES pH 7.9, 10 mM KCl, 2 mM Mg acetate, 3 mM CaCl2, 340 mM sucrose, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA to 1× ...
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bioRxiv - Molecular Biology 2022Quote: ... The 5’ and 3’ linkers were terminally labelled with digoxigenin-11-dUTP (Roche) or biotin-16-dUTP (Roche) ...
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bioRxiv - Biophysics 2022Quote: GGCAGCGCTACCATAACGGA-3’) (11) by RT-qPCR was performed using LightCycler 480 II (Roche). GAPDH mRNAs were used as a loading control (14) ...
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bioRxiv - Molecular Biology 2019Quote: ... washed once with ice cold Phy Buffer (150 mM Potassium Acetate, 5 mM Magnesium Acetate, 20 mM HEPES-KOH pH 7.4, 5 mM DTT, protease inhibitor cocktail [Roche], 10 µg/mL cycloheximide). Cell pellets were then re-suspended in 1 mL Phy Buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected according to the manufacturer’s protocol at a µL lipofectamine: µg plasmid ratio of 3:1 (X-tremeGENE 9, Roche). After 48 hours ...
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bioRxiv - Microbiology 2023Quote: ... and S-D-Bact-0785-a-A-21 (GACTACHVGGGTATCTAATCC) (Klindworth et al. 2013) together with KAPA HIFI Hot Start Ready Mix (Kapa Biosystems, Cat. No. 07958935001) containing the polymerase with proofreading activity ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP (Roche, 11 814 460 001, 1:500). Secondary antibodies used were anti-mouse IgG-HRP (Dako ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP (Roche 11 814 460 001, 1:500), TRF1 (Rabbit ...
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bioRxiv - Neuroscience 2023Quote: ... HA (1:100, rat, Roche #11-867-423), and GFP (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... plasmid DNA from the in vitro methylation reactions were transfected with 3 µL (Il33) or 1 µL (SV40) X-tremeGENE 9 transfection reagent (Roche) diluted in 50 µL of Opti-MEM medium (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... and pBlueScript II KS reverse (5’-GCT GGG TCT CGT GGT TTC CCT TTA GTG AGG GTT AAT TG) primers and either 60 µM biotin-16-dUTP or digoxigenin-11-dUTP (Roche). This results in a 5 kb DNA tether which can be attached to a streptavidin coated magnetic bead at one end and an anti-digoxigenin coverslip surface at the other.
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bioRxiv - Biophysics 2021Quote: ... including 200 μM final concentration of each dNTP (G,C,T,A) and 10 µM Bio-16-dUTP or Dig-11-dUTP (all from Roche). Labelled handles specifically bind either to a glass surface covered with anti-digoxigenins or to superparamagnetic beads covered with streptavidin ...
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bioRxiv - Cancer Biology 2019Quote: ... The two DNA handles (510bp) labeled by multiple digoxigenin and biotin are generated by mixing biotin-16-dUTP and digoxigenin-11-dUTP nucleotides (Roche) with dNTP solution mix in the respective PCR reaction ...
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bioRxiv - Cell Biology 2022Quote: ... 5’-CAA GTG CAA CAG TTT CTC ATT-3’/5’-TGT TTG ACT ACA CTC ACA CT-3’) using X-tremeGENE 9 DNA Transfection Reagent (Roche). 48 hours after transfection ...
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bioRxiv - Developmental Biology 2022Quote: ... 3 µL 10% Tween-20 (Roche 11332465001), freshly add 3 µL 1:1 water diluted digitonin (Promega G9441) ...
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bioRxiv - Genomics 2019Quote: ... DIG-11-dUTP deoxyribonucleotides (Roche 11 175 033 910) and gel purified ...
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bioRxiv - Genomics 2022Quote: ... with digoxigenin 11-dUTP or biotin 11-dUTP (Roche) using the linearised clone pTa71 (from Triticum aestivum ...
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bioRxiv - Genomics 2020Quote: ... 1× Protease Inhibitor Cocktail (Roche, 11 873 580 001)] and incubation at 12 °C on a ThermoMixer for 30 min ...