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Citations for Roche :
1251 - 1269 of 1269 citations for Recombinant Mouse SHH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... the expression medium containing the secreted fusion protein was supplemented with 1/4 tablet of protease inhibitor (complete EDTA-free protease inhibitor cocktail tablets, Roche Diagnostic GmbH, 45148300) and transferred to a 15 ml Falcon tube containing 200 μl of washed ...
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bioRxiv - Cell Biology 2020Quote: ... 50 μg of proteins from the microsomal membrane pellet (P13) were incubated with 30 μg/ml of proteinase K (Roche Diagnostics, Mannheim, Germany) for 30 min on ice ...
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bioRxiv - Developmental Biology 2022Quote: Testes from adult mice were cut into ~20 mg pieces and transferred into ~200 μl pre-cooled denaturing buffer from the Minute Total Protein Extraction Kit (SD-001, Invent Biotech, Plymouth, MN, USA) containing protease inhibitors (CO-RO, Roche, Indianapolis, IN, USA) and phosphatase inhibitors (PHOSS-RO ...
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bioRxiv - Cell Biology 2023Quote: ... were used to remove salts from purified protein and to exchange to freezing buffer (20mM HEPES, 100mM NaCl, 0.5mM EDTA, TCEP 0.5mM and Roche cOmplete™ Protease Inhibitor Cocktail), using the gravity protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue extracts were subjected to TCA precipitation as previously described53.Washed protein pellets were solubilized in 900 μl of urea buffer (8M urea, 150mM NaCl, 50mM Tris pH8, 1X Roche complete protease inhibitor). Protein quantitation was performed using Qubit fluorometry (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... The elute was neutralized with a PBS-diluted protein inhibitor cocktail (cOmplete, EDTA-free, protease inhibitor cocktail tablets: Roche Diagnostics GmbH, Mannheim, Germany) and used to infect TG1 cells ...
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bioRxiv - Cell Biology 2020Quote: ... In addition, the goat polyclonal antibody anti-Cta1p (Kragler et al., 1993) and the mouse monoclonal antibodies anti-GFP (Roche Diagnostics, cat. # 11814460001), anti-Pgk1p (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... Slides were blocked in 10% BSA and 0.05% Tween 20 in PBS for 30 minutes before application of one of the following antibodies: mouse anti-human p16INK4a (CINTEC-Roche, cat# 705-4793), rabbit anti-human alpha-smooth muscle actin or aSMA (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... The remaining right lungs and spleens of each A/J mouse treated with PBS or BNF 0.0005mg were macerated with protease inhibitor (complete, EDTA-free, Roche Life Science, IN, USA) and centrifuged ...
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bioRxiv - Cell Biology 2023Quote: RNA was obtained from epithelioids 1 week after medium change to mFAD or from mouse esophageal epithelium peeled and incubated with Dispase I (Roche catalog no. 04942086001) diluted at 1 mg/ml in PBS for 15 min ...
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bioRxiv - Pathology 2023Quote: FDB muscles were harvested bilaterally in sterile mouse ringer and maintained overnight in DMEM (supplier) supplemented with collagenase A (Roche, 0.2 mg/ml) and 1% Penicillin- Streptomycin in a CO2 incubator (37°C ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 1 hour incubation with the following primary antibodies diluted in blocking solution: mouse IgG1 mAb α-GFP (1:500, Roche Diagnostics #11814460001), mouse IgG2 mAb α-Centrin clone 20H5 (1:500 ...
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bioRxiv - Neuroscience 2019Quote: Synaptosomes were prepared using the Procedure for Synaptic Protein Extraction from Neuronal Tissue” and Syn-PER Synaptic Protein Extraction Reagent (Thermo Fisher, #87793, containing a half tablet of cOmplete™ Protease Inhibitor Cocktail (Roche, #CO-RO) per 12.5 ml Syn-PER reagent ...
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bioRxiv - Biochemistry 2020Quote: ... The gel was stained with Coommassie Blue and the single band of proteins was excised and subjected to in-gel digestion with bovine trypsin (Roche Diagnostics GmbH, Mannheim, Germany) as previously described by Marie et al ...
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bioRxiv - Biochemistry 2023Quote: ... 100 ng of recombinant PfCDPK1 protein was taken in a kinase assay buffer (50 mM Tris, 50 mM MgCl2, 1× phosphatase inhibitor cocktail (Roche Life Science, Indianapolis, IN), 1 mM DTT ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The unreacted compound was removed via centrifugation using a molecular weight cutoff filter or G-25 Sephadex Quick Spin Protein columns (Roche Applied Science, Indianapolis, IN).
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bioRxiv - Pathology 2020Quote: ... were performed at the National Mouse Metabolic Phenotyping Center (MMPC) at the University of Massachusetts Medical School using a Cobas Clinical Chemistry Analyzer (Roche Diagnostics, Indianapolis, IN, USA) while plasma non-esterified fatty acid (NEFA ...
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bioRxiv - Molecular Biology 2023Quote: ... Classically activated macrophages were prepared by stimulating macrophages with 10 ng/mL lipopolysaccharide (LPS) (L5293, SigmalAldrich) and 10 ng/mL mouse interferon-γ (mIFN-γ, 11276905001, Roche, Basel, Switzerland). In loss-of-function experiments ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10, 1:50, Roche, or rabbit anti-FLAG, 1:1000, SIGMA) followed by incubation with Alexa488- and/or Alexa647-coupled secondary antibodies (Molecular Probes ...