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Citations for Roche :
1001 - 1050 of 1994 citations for Arylacetamide Deacetylase Like 4 AADACL4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... centrifuged for 5 min at 1000 rpm and stored at 4 °C until measurement for LDH activity according to the manufacturers protocol (Roche, LDH Cytotoxicity Detection Kit ...
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bioRxiv - Neuroscience 2024Quote: ... Whole mount larvae and adult brain sections layered on glass slides were blocked for at least one hour in PBT with 2 mg/ml bovine serum albumin and 2% sheep serum at room temperature and then incubated overnight at 4°C with alkaline phosphatase-coupled anti-DIG antiserum (11093274910, Roche) diluted 1/5000 in blocking solution ...
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bioRxiv - Molecular Biology 2023Quote: ... and nuclei extracted overnight at 4 °C in 0.35M/0.6M buffer supplemented with a protease inhibitor cocktail (Roche Cat #05056489001). ChIP was performed with anti-H3K4me2/ anti-H3K9me3 antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... The structure of the lacO–tetO reporter locus was checked by a set of 4 PCRs using Expand Long Template PCR System (Roche) and the following primer pairs ...
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bioRxiv - Molecular Biology 2022Quote: ... blots were hybridized with 10 pmol/ml DIG-labeled (CAG)7 (5′-gcAgCagcAgca-3′) at 70°C for 4 h in hybridization buffer (5 × SSC, 1% block solution [Roche, Switzerland] ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was hybridized with a TAA(CCCTAA)4 probe which was conjugated with digoxigenin (DIG oligonucleotide 3⍰-end labelling kit, Roche) and signal was revealed using the anti-DIG-alkaline phosphatase antibodies (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed once with PBS and resuspend with Tris-HCl containing 4 mM EDTA and 1× complete protease inhibitor cocktail (Roche). Dounce homogenizer is used to fractionate the cell membranes and then 3 min 600 g centrifugation was used to obtain Post-Nuclear Supernatants (PNS) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... in PBS for 30 min and incubated overnight at 4°C with 1:1,500 anti-HA high affinity (Roche, 3F10). After three PBS washes ...
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bioRxiv - Neuroscience 2023Quote: ... diluted in PBS for 2 hours at room temperature after which they were counterstained with 300 nM 4’,6’-diamino-2-phenylindole (DAPI; 10236276001, Roche) for 5 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.1% Tween 20 in water) and incubated in NBT/BCIP (nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate) solution (Roche).
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bioRxiv - Cell Biology 2023Quote: Cells were lysed in SDS lysis buffer (4% SDS, 20% glycerol, 125 mM Tris-HCl pH=6.8) supplemented with protease inhibitors (cOmplete ™ Mini (Roche)) ...
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bioRxiv - Neuroscience 2023Quote: Pools of 2 to 4 organoids were resuspended in cold lysis buffer (Cell Signalling Technology) supplemented with protease and phosphatase inhibitor cocktails (Roche) and sonicated twice for 3 seconds at 10% intensity in the Branson digital Sonifier SFX 150 (Emerson) ...
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bioRxiv - Bioengineering 2023Quote: ... SOS1•RAS complex was formed by incubating SOS1 and RAS at a stochiometric ratio of 1:3 overnight at 4° with 20 mM EDTA and alkaline phosphatase (Roche). The complex was then purified by gel filtration ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were then washed 2 × 5 min at 4 °C with cold 1 × PBS containing protease inhibitor cocktail (Roche, #4693132001), snap frozen and stored at −80°C until awaiting further processing ...
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bioRxiv - Molecular Biology 2023Quote: ... Alkaline phosphatase activity was detected in the dark in AP buffer supplemented with 45 mg/ml 4-nitrobluetetrazolium chloride (NBT, Roche) and 35 mg/ml 5-bromo-4-chloro-3-indolyl-phosphate (BCIP ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were blocked for 30 minutes in 1% Roche Western Blocking Reagent prior to incubating overnight at 4°C with either anti-FITC with horseradish peroxidase conjugate (Roche) at a 1:2000 concentration or anti-DIG-with horseradish peroxidase conjugate (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... slides were incubated at 37oC in a staining solution containing nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate (Roche) for 20-24 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... Probes were synthesized at 37°C for 2–4 hrs in digoxigenin-synthesis reaction mixture with T7 RNA polymerase (Roche). After synthesis ...
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bioRxiv - Pathology 2023Quote: ... Bound alkaline phosphatase was visualized with nitroblue tetrazolium chloride and 5-bromo-4-chloro-3-indolyl-phosphate (NBT/BCIP; 11681451001, Roche). The reaction was stopped by incubation in buffer 4 (10 mM Tris and 1 mM EDTA ...
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bioRxiv - Genomics 2023Quote: One hundred mg of 14-day-old seedlings were ground and nuclei were isolated with 4°C Buffer (0,25M Sucrose, 10mM Tris-HCl, 10mM MgCL2, 1% Triton, 5mM ß- mercaptoethanol) containing proteinase inhibitor cocktail (Roche) and filtered in 63 µm ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were pelleted by centrifugation (750 x g, 2 min, RT) and washed in 4 ml vPBS containing EDTA-free protease inhibitor (Roche). The washed cells were again pelleted by centrifugation (750 x g ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were pelleted by centrifugation (750 x g, 2 min, 4 °C) and resuspended in 50 μl vPBS containing EDTA-free protease inhibitor (Roche). The cells were fixed by addition of 0.5 ml ice-cold fixation solution (4% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... The larvae were then incubated overnight at 4°C with anti-Dig-AP (1:2000 in 5% normal goat serum, 11093274910, Roche) and washed before detecting alkaline phosphatase using NBP/BCIP(Roche ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed three times with 1X PBS before nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, Roche) for 10 min at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 0.4–0.5 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche), and the cells were fixed 5–6 days later ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were first incubated with 5 µl anti-GFP antibody (in house) for 1 hour rotating at 4°C and subsequently mixed with 50 µl protein G-agarose (50% slurry, Roche) and incubated for 1 hour rotating at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... and stressed (n=4) DA neurons to prepare stranded RNAseq libraries following manufacturer’s recommendations using KAPA mRNA hyperprep (Roche Diagnostic). Each final library was quantified and qualified with 2200 Tapestation (Agilent) ...
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bioRxiv - Biochemistry 2024Quote: ... 2.5 mL of lysis buffer (50 mM MES, pH 6.5 at 4 °C, plus one cOmplete™ EDTA-free protease inhibitor cocktail tablet (Roche) per 50 mL ...
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bioRxiv - Evolutionary Biology 2024Quote: ... bmp2/4 and bmp5–8 was performed using traditional cloning methods and visualised with NBT/BCIP stock solution (Roche 11681451001), as previously described143.
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bioRxiv - Immunology 2024Quote: Cell lysates were prepared by lysing cells for 1 h at 4 °C in RIPA buffer supplemented with cOmplete protease inhibitor cocktail (Roche). The cell lysate concentrations were quantified using a BCA protein assay kit (ThermoFisher) ...
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bioRxiv - Plant Biology 2019Quote: ... IP was conducted using the anti-GFP antibody (Roche, 11814460001, Lot: 19958500) and Pierce Protein G magnetic beads (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies conjugated with HRP and Lumi-Light Western Blotting Substrate (Roche) were used to visualize specific protein bands ...
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bioRxiv - Cell Biology 2020Quote: Antibodies used in this study included mouse monoclonal anti-GFP (Roche Diagnostics), rat anti-HA (Roche Diagnostics) ...
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bioRxiv - Developmental Biology 2021Quote: ... Then sections were incubated with Anti-DIG-AP fab antibody (Roche, 11093274910) prepared in blocking buffer overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... The secondary antibody solution was then replaced with DAPI solution (Roche, 10236276) in the dark at room temperature for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary and secondary antibody for FISH were sheep anti-digoxigenin (Roche 11333089001) 1:375 ...
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bioRxiv - Developmental Biology 2021Quote: ... Hybridized probes were detected using anti-DIG-POD antibody (1:1000, Roche), anti-DNP-HRP antibody (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... 50mL supernatant and 1mL of anti-HA-HRP antibody (Roche, cat#12013819001) solution (1:500 ...
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bioRxiv - Developmental Biology 2021Quote: ... Hybridization was detected using anti-DIG antibody conjugated with alkaline phosphatase (Roche) and color was developed with 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium (BCIP/NBT ...
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bioRxiv - Microbiology 2021Quote: ... then incubated with biotin-conjugated anti-rat antibody (Roche, diluted 1:8,000) in blocking buffer followed by horseradish peroxidase-conjugated streptavidin (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... All other antibodies were commercially available: mouse αGFP (Roche, 11814 460 001), rabbit αGFP rabbit (life technologies ...
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bioRxiv - Cell Biology 2019Quote: ... followed by SDS-PAGE and Western Blotting using anti-GFP antibody (Roche), HRP-conjugated anti-mouse secondary antibody (Roche ...
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bioRxiv - Microbiology 2019Quote: ... Antibody concentrations used were: rat anti-HA high affinity (Roche, Basel, Switzerland) (1:1000) ...
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bioRxiv - Molecular Biology 2019Quote: ... Immunoprecipitation was performed using 10 µg of anti-GFP antibody (Roche, 11814460001) (used for Hap2-GFP IP ...
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bioRxiv - Biochemistry 2019Quote: ... The membrane was analyzed using primary antibodies to GFP (1:1,000; Roche) and anti-porin (1:1,000 ...
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bioRxiv - Cell Biology 2019Quote: ... Cleared lysates were subjected to immunoprecipitation using anti-GFP antibody (Roche 11814460001) conjugated to protein G magnetic beads (Thermofisher ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies used for immunoprecipitation were Protein G Agarose beads (Roche, 1124323301) and anti-FLAG (M2 ...
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bioRxiv - Neuroscience 2020Quote: ... the fluorescein probe was detected with an anti-fluorescein primary antibody (Roche) and a goat anti-mouse-HRP secondary antibody (Invitrogen) ...
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bioRxiv - Neuroscience 2019Quote: ... The probe was detected with an anti-DIG antibody (Roche; 1:10,000) and visualized using CDP-star substrate (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... MRG-1::3XHA was detected with rat anti-HA-HRP antibody (Roche) at a dilution of 1:500 ...