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Citations for Roche :
751 - 800 of 1374 citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Beads were washed three times with 200 µL of washing buffer (50 mM Tris-HCl at pH 7.5, 150 mM KCl, 5 mM MgCl2, 0.05% NP-40, EDTA-free cOmplete mini protease inhibitor [Roche, 11836153001] ...
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bioRxiv - Immunology 2023Quote: ... HEK293T cells were lysed in LUMIER lysis buffer (50 mM Hepes-KOH pH 7.9, 150 mM NaCl, 2 mM EDTA, 0.5% Triton X-100, 5% glycerol and Roche cOmplete™ Mini protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... 80 μL DNase I (5 mg/ml; DNase I – grade II from bovine pancreas from Roche (Basel, Switzerland)) ...
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bioRxiv - Microbiology 2023Quote: ... after which they were resuspended in 1.0 ml of ice-cold lysis buffer (10 mM Tris-HCl, pH 7.7; 10 mM NaCl; 5 mM MgCl2; 0.1 mM EGTA; protease inhibitors [Roche]) and incubated on ice for 10 min before nuclei were pelleted (400 × g ...
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bioRxiv - Neuroscience 2023Quote: ... Bacterial pellets were collected via centrifugation at 4°C and 5,000g and resuspended in ice-cold lysis buffer (50 mM MES pH 6.8, 1 mM EGTA, 0.2 mM MgCl2, 5 mM DTT, Roche complete protease inhibitor ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA extracted from the parasites at passages 7 and 20 were digested with 5 U Rsa1 (Roche) at 37 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.9; 5 mM MgCl2; 0.2% Triton X-100; 20% glycerol; 300 mM NaCl and freshly added ROCHE cOmplete protease inhibitor cocktail at 0.01 tablet/mL) ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µl cDNA and 5 µl of LightCycler 480 SYBR Green I Master mix (Roche Diagnostics GmbH, Germany). Three technical replicates of each sample were included ...
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bioRxiv - Physiology 2024Quote: ... 5 mM EDTA, 2% Triton-X-100, 0.2 mM HDSF, pH 7.4, and protease inhibitor cocktail from Roche). Following sonication on ice ...
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bioRxiv - Plant Biology 2024Quote: ... Tissues were ground with mortor and pestle and resuspended in 30 mL extraction buffer 1 (10 mM Tris-HCl, pH 8.0, 5 mM βmercaptoethanol, 0.4 M sucrose, protease inhibitor cocktail (cOmplete, Roche), vortexed ...
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bioRxiv - Developmental Biology 2024Quote: ... The binding reactions were separated on native 5% acrylamide gels and transferred onto positively charged nylon membranes (Roche) with a Perfect Blue Semi-dry electro blotter (60 minutes at 120volts ...
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bioRxiv - Molecular Biology 2024Quote: ... 1mM MgCl2, 0.1% NP-40, 5 mM DTT, 0.5 mM PMSF, 1× EDTA-free protease inhibitor cocktail [Roche] ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 mM Tris-HCl pH 8.0, 5 mM EDTA, 0.5 % SDS, 1#x00D7; protease inhibitor cocktail from Roche). The resulting nuclei were spun down ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mL of Farnham lysis buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40, EDTA-free Roche protease inhibitor tablets ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in lysis buffer (50 mM HEPES, pH7.5, 100 mM KCl, 5% glycerol, 0.3% Triton X-100, 1X Roche EDTA-free Complete Protease Inhibitor cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in lysis buffer (50 mM HEPES, pH7.5, 100 mM KCl, 5% glycerol, 0.3% Triton X-100, 1X Roche EDTA-free Complete Protease Inhibitor cocktail ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mL of the culture was then treated with either 5 µl of MeOH or LMB dissolved in 7:3 MeOH:H2O solution (Roche) at a final concentration of 50 ng/mL for 45 min before imaging.
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bioRxiv - Cell Biology 2022Quote: ... Cells were harvested using ice cold EBC buffer (140 mM NaCl, 50 mM Tris-HCl, 0.5% NP-40, 5 mM EDTA and protease inhibitor cocktail (Roche)) and briefly sonicated prior to 20 min centrifugation at 20,000 g ...
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bioRxiv - Molecular Biology 2021Quote: Mouse livers were homogenized with a motorized tissue grinder in STM buffer (Tris-HCl pH 7.4, 5 mM MgCl2, 250 mM Sucrose, 1 mM DTT, supplemented with 500 µM PMSF, Protease Inhibitor Cocktail [Roche, Basel ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed in 500 μl lysis buffer (50mMTris-HCl, pH8.0, 100mM NaCl, 10m MEDTA, 5% glycerine, 1% NP- 40 and protease inhibitor cocktail (Roche)) for 40 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 mM NaCl, 5 mM MgCl2, 0.5% NP-40, 1 mM DTT, and 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]), washed once with PBS ...
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bioRxiv - Cell Biology 2022Quote: ... 5 mM EDTA) with the addition of a Complete EDTA free protease inhibitor cocktail tablet (Roche Diagnostics, Oslo, Norway) and a PhosSTOP tablet (Roche) ...
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bioRxiv - Neuroscience 2019Quote: ... cells were collected and lysed with a lysing buffer (100mM KCl, 20mM HEPES, 5% Glycerol, 0.1X Triton-X, 1mM DTT, and Complete protease inhibitor (Roche)) ...
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bioRxiv - Genetics 2019Quote: ... DNA was eluted in 100μl of TE + 1%SDS and the beads were washed and eluted a second time with 100μl of TE + 1%SDS + 5 μl of 20mg/mL Proteinase K (Roche). Following overnight incubation at 65°C ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM MgCl2, 1 mM EGTA, 0.1% [v/v] NP-40, 1 mM DTT, 5% [v/v] glycerol, Roche Complete Protease Inhibitors and PhosSTOP Protein Phosphatase Inhibitors ...
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bioRxiv - Neuroscience 2019Quote: ... Resin was washed with TBS buffer (150 mM NaCl, 20 mM Tris-Cl, pH 7.4, 5 mM MgCl2, EDTA free protease inhibitors (Complete, Roche) and incubated with purified 6xHis-tag LC3 for 120 min @4°C in TBS buffer either alone or in the presence of purified 6xHis-Snapin ...
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bioRxiv - Molecular Biology 2019Quote: ... were lysed in IP buffer (10 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM PMSF and Roche complete EDTA free protease inhibitor cocktail) ...
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Genomic organization of the autonomous regulatory domain of eyeless locus in Drosophila melanogasterbioRxiv - Genetics 2021Quote: ... The fixed embryos were resuspended in 2.5 ml of ice-cold lysis buffer (10 mM Tris-pH-8, 10 mM NaCl, 0.2% NP40 with Roche protease inhibitor cocktail freshly added ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatants from each buffer were dialyzed in 25 mM Tris-HCl and 5 mM EDTA pH 8.0 overnight at 4°C and subsequently digested with 200 mg/ml pronase (Roche). Peptides were precipitated with 5% trichloroacetic acid (TCA ...
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bioRxiv - Neuroscience 2020Quote: ... Each tissue sample was dissociated using 2.4 mL of homogenization buffer (10 nM Tris pH 8, 5 mM MgCl2, 25 mM KCl, 250 mM sucrose, 1 μM DTT, 0.5x protease inhibitor [cOmplete, Roche #4693159001] ...
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bioRxiv - Neuroscience 2020Quote: ... 5’-CAGACACGCAGACTCTTTC-3’) and 2.5 µl reverse primer (10 µM; 5’-CTAAAGATGTGTGTCTTCCTCA-3’) using the Expand Long Template PCR System (Roche). The PCR conditions were 35 cycles at 95°C for 30 sec ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were harvested and incubated in buffer A (50 mM MMT pH 8.0/300 mM KCl/10 mM MgCl2/5 % glycerol) containing 1 g L-1 lysozyme/2.5 U mL-1/SmDNAse//complete protease inhibitor cocktail (Roche) for 1 h prior to lysis using EmulsiFlex C5 (Avestin ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 mM KCl, 5 mM MgCl2, 0.01% NP-40, freshly prepared 20 mM Na2MoO4, and cOmplete protease inhibitors; Roche). 10-20 million cells were lysed in 0.5 mL Felts buffer by rotation at 4°C for 30 minutes and vortexing for 10 seconds ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei pellets were resuspended in 1 ml nuclei permeabilization buffer (5 % BSA, 0.2 % IGEPAL-CA630, 1mM DTT and cOmpleteTM, EDTA-free protease inhibitor cocktail (Roche) in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... which were blocked and permeabilized with 5% normal goat serum in 0.25% Triton X-100 in PBS (Roche Diagnostics) for 30 min at 25°C ...
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bioRxiv - Plant Biology 2021Quote: ... Each 10 μl reaction was comprised of 5 μl LightCycler 480 SYBR Green I Master mix (Roche Life Science), 2 μl cDNA template ...
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bioRxiv - Biochemistry 2021Quote: ... Pelleted cells were resuspended in 1x PBS buffer (pH 7.4) with 5 mM BME and proteinase inhibitor cocktail (Roche). For purification of PABPC1-His ...
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bioRxiv - Biochemistry 2020Quote: ... re-suspended with buffer A (50mM Phosphate buffer pH 8, 400mM NaCl, 5% glycerol, 1mM DTT, 0.5mM EDTA, protease inhibitor cocktail from Roche) and lysed using a microfluidizer followed by two cycles of centrifugation (12000 × g 20 min) ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then washed 3 times for 5 min each in PBST and blocked with 1X Blocking Reagent (Roche) in PBST ...
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bioRxiv - Microbiology 2020Quote: ... cells were centrifuged and pellets were resuspended in RB1 (20mM Tris pH7.5, 300mM NaCl, 1mM DTT, 5% glycerol and protease inhibitor (complete EDTA-Free, Roche)) ...
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bioRxiv - Plant Biology 2020Quote: ... 0.5 mM PMSF) supplemented with 5 mg L−1 DNAse I and 0.5 g/L lysozyme (Roche, Mannheim, Germany). Cells were disrupted by sonication (3×2 min ...
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Plant pathogens convergently evolved to counteract redundant nodes of an NLR immune receptor networkbioRxiv - Plant Biology 2021Quote: ... 5% (vol/vol) glycerol and 20 mM imidazole (buffer A) supplemented with cOmplete EDTA-free protease inhibitor tablets (Roche) and lysed by sonication ...
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bioRxiv - Plant Biology 2021Quote: ... 500 nM of each primers and 5 μl of 2X LightCycler® 480 SYBR Green I Master mix (Roche) in 10 μl final volume ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was diluted 5 times and subjected to real-time PCR using LightCycler 480 SYBR Green I Master (Roche) with specific primers for the genes of interest ...
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bioRxiv - Microbiology 2021Quote: ... 60 mM KCl, 1 mM CaCl2, 5 mM MgCl2, 300 mM sucrose, 0.4% NP-40, 1 mM DTT, 1x Roche Complete protease inhibitors cocktail EDTA-free ...
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bioRxiv - Plant Biology 2021Quote: ... 150 mM NaCl, 10% glycerol, 0.2% NP-40, 1 mM EDTA, 5 mM DTT, and EDTA-free protease inhibitor [Roche]). Anti-GFP agarose resin (Chromotek ...
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bioRxiv - Microbiology 2021Quote: ... 109 CFUs of bacteria were resuspended in 150 µL of buffer TE (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) with 5 mg mL-1 of lysozyme (Roche) for 5 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei pellets were resuspended in 1 ml nuclei permeabilization buffer (5 % BSA, 0.2 % IGEPAL-CA630, 1mM DTT and cOmpleteTM, EDTA-free protease inhibitor cocktail (Roche) in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... 150 mM NaCl, 50 mM Tris pH 8.5, 40 mM chloroacetamide, 5 mM TCEP; freshly added: 1 tablet Phosphostop [Roche, 4906845001] and 1 tablet cOmplete Protease inhibitor cocktail [Roche ...
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bioRxiv - Cell Biology 2020Quote: ... cells from mouse blood infected with the DEH-GFP-expressing parasite were pelleted and then lysed in hypotonic buffer (10 mM Tris-HCl pH 8.4, 5 mM EDTA) containing protease inhibitors (Roche), freeze/thawed twice ...