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Citations for Roche :
5601 - 5650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... followed by library preparation using the Kapa RNA HyperPrep kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and dispase II (Roche, Mannheim, Germany) for 10 min at 37° C ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed on the resulting cDNA using a LightCycler 96 Real-Time PCR system (Roche) and Luna Universal qPCR Master Mix kit (New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... Both lysis buffers were supplemented with cOmplete Mini EDTA-free protease inhibitor cocktail (Roche) and Benzonase (Merck Millipore ...
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bioRxiv - Microbiology 2023Quote: ... and 0.1% Triton X-100 supplemented with 1 mM PMSF and one Complete Protease Inhibitor tablet (EDTA-free; Roche), and then centrifuged at 13,000 × g for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... with the LightCycler® 480 SYBR Green I Master Mix (2X, Roche) and the primer pairs RppkF/RppkR for Lc ...
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bioRxiv - Microbiology 2023Quote: ... 10 μg/ml DNase I (Roche), 20 μg/ml RNase A (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... Real-time qPCR was performed using a LightCycler®480 Real-Time PCR system (Roche Diagnostics, USA) with the LightCycler® 480 SYBR Green I Master Mix (2X ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed once with PBS and then lysed with 0.5% NP-40 in PBS supplemented with cOmplete Mini EDTA-free protease inhibitor cocktail (Roche) and Benzonase (Merck Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... lysed in Laemmli buffer (62.5 mM Tris-HCl, pH 6.8, 10% glycerol, 2% SDS) supplemented with Complete® protease inhibitors (Roche), and sonicated before protein quantification (DC BioRad Protein Assay Cat No ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.25 or 0.17 mg ml−1 Liberase (Roche Diagnostics). The digested lungs were passed through a 1 ml syringe to make single-cell suspensions ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus was produced in 293T cells transfected using X-tremeGENE HP (Roche) with viral constructs along with VSV-G and gag-pol ...
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bioRxiv - Immunology 2023Quote: ... streptavidin-AP conjugate (Roche), SIGMAFAST BCIP/NBT (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... for GFP and mCherry or 1 mg/mL G-418 solution (Roche, 04727878001) for luciferase ...
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bioRxiv - Immunology 2023Quote: ... the medium was supplemented with 30 IU/mL recombinant human interleukin 2 (IL-2) (Proleukin, Roche). Electroporated T cells were kept in culture ON and then used directly or frozen.
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bioRxiv - Immunology 2023Quote: ... and 0.25 mg/ml of Liberase TL purified enzyme blend (Roche) for 90 minutes at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... and 50 ng/mL recombinant DNase I (Roche Diagnostics) in PBS ...
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bioRxiv - Immunology 2023Quote: ... All libraries were quantified via Agilent 2100 hsDNA Bioanalyzer and KAPA library quantification kit (Roche, Cat. No. 0796014001.) Gene expression libraries were sequenced at a targeted depth of 25,000 reads per cells ...
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bioRxiv - Immunology 2023Quote: ... spleens from wild-type or Axl-/- mice were digested with 500 μg/mL Liberase (Roche Diagnostics) and 50 ng/mL recombinant DNase I (Roche Diagnostics ...
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bioRxiv - Immunology 2023Quote: ... 5 mM CaCl2 and 5 mM MgCl2 with 100× concentrated EDTA-free protease inhibitor cocktail (Roche, pallet). The suspensions were treated with Dounce and added with 25 mU ml−1 apyrase ...
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bioRxiv - Immunology 2023Quote: ... Whole transcriptome amplification (WTA) was achieved via PCR using KAPA Hifi PCR Mastermix (Kapa Biosystems). The WTA product was purified using Agencourt Ampure beads (Beckman Coulter) ...
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bioRxiv - Immunology 2023Quote: ... the hydrogels were carefully collected using cut 1000-µL pipette tips and incubated 15 minutes at 37°C in a 0.5 mg.mL-1 Collagenase (Roche, 11088866001) solution in DMEM/F12 ...
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... Terminal transferase (2 µl/ml; Roche, Basel, Switzerland) and biotinylated dUTP (1 µl/ml ...
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bioRxiv - Physiology 2023Quote: ... and 1% Pen/Strep (11074440001, Roche).
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bioRxiv - Neuroscience 2023Quote: ... The KAPA Hyper Prep DNA library kit (KAPA Biosystems, Wilmington, MA, USA) was used to prepare the libraries ...
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bioRxiv - Molecular Biology 2023Quote: ... We then prepared libraries from these extractions with a Hyper library kit (Kapa Biosystems) and sequenced them on an Illumina NovaSeq 6000 with 150bp paired-end reads [32] ...
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bioRxiv - Microbiology 2023Quote: Cells were washed in PBS and harvested in lysis buffer (1% NP-40, 2mM EDTA, 10% glycerol, 150mM NaCl, 50mM Tris-HCl, protease inhibitor (Roche complete mini EDTA-free protease inhibitor) ...
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bioRxiv - Cell Biology 2023Quote: ... and 2 μl of RNase-free DNase I (20 μ/ml, Roche) were added to the washed antibody beads and were precipitated ON rotating at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Gene-specific primers and probes (Universal ProbeLibrary, Roche) were used in combination with qPCR Probe-MasterMix (SL-9802 ...
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bioRxiv - Cancer Biology 2023Quote: Real-time quantitative PCR (qPCR) was performed in accordance with MIQE-guidelines (15) using a LightCycler® 480 Real-Time PCR Instrument (Roche, Mannheim, Germany) in a 384-well plate format ...
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bioRxiv - Synthetic Biology 2023Quote: ... or KAPA HiFi HotStart ReadyMix (Roche), cloned the gene library into the yeast surface display vector pCTCON2 (provided by Dane Wittrup ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5% NP-40 and 10% glycerol) containing protease inhibitor cocktail (cOmplete Roche, 04693159001), followed by end-over-end rotation at 4 °C for 6 hours ...
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bioRxiv - Immunology 2023Quote: ... antigen retrieval was performed using a tris/borate/EDTA buffer (Discovery CC1, 06414575001; Roche), pH 8.0 to 8.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM EGTA and cOmplete Protease Inhibitor Cocktail (Roche)) and centrifuged at 1500g for 2 min at 4°C ...
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bioRxiv - Physiology 2023Quote: ... Body weight was monitored weekly and blood glucose was assessed with a glucometer (ACCU-CHEK Aviva, Roche, UK) at 2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... supplemented with a protease inhibitor cocktail (Roche)) and incubated with 25 mU / mL apyrase for 1 h at RT ...
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bioRxiv - Cell Biology 2023Quote: ... cOmplete protease inhibitor cocktail (Roche), phosphatase inhibitor cocktail 1 and 2 (Sigma-Aldrich)) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Reverse transcription was performed using first-strand cDNA synthesis kit for RT-PCR (Roche Diagnostics, Basel, Switzerland). qPCR was then performed on a LightCycler™ (Roche Diagnostics ...
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bioRxiv - Neuroscience 2023Quote: ... and Phos-STOP (Roche). Protein concentration was determined by the Bradford assay ...
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bioRxiv - Plant Biology 2023Quote: Quantitative real-time PCR (qRT-PCR) analysis was performed using the KAPA SYBR FAST qPCR kit (KAPA Biosystems Inc., Switzerland) to check the gene expression levels of GLXI;1 ...
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bioRxiv - Physiology 2023Quote: ... a protease inhibitor (1 minitab per 10 mL, Roche), pH = 7 ...
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bioRxiv - Physiology 2023Quote: ... Worms were homogenized in PBS containing 5% TritonX-100 and a protease inhibitor cocktail (Roche), and lipid was extracted using the TissueLyser II (QIAGEN ...
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bioRxiv - Physiology 2023Quote: ... cells/tissue were lysed in mammalian protein extraction reagent (M-PER) supplemented with protease inhibitor cocktail (Roche). Soluble proteins and insoluble matrix were separated by centrifugation at 10,000 × g ...
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bioRxiv - Bioengineering 2023Quote: ... and dispase (Roche, 0.03 U/mL) in Dulbecco’s Modified Eagle’s medium (DMEM ...
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bioRxiv - Neuroscience 2023Quote: ... in a 1:1 ratio and added to white 384 well plates (Roche). Plates were run on a 45-cycle protocol using the LC 480 II system (Roche).
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bioRxiv - Neuroscience 2023Quote: ... and a High Pure RNA tissue kit (Roche). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... and dNTPs (Roche).
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bioRxiv - Neuroscience 2023Quote: ... chopped samples were transferred to a 15 ml Dounce Homogenizer with 5ml homogenisation buffer (100 μM of DTT [Sigma–Aldrich, St. Louis, MO, USA], 0.1% Triton X-100 [Sigma–Aldrich], 2X EDTA Protease Inhibitor [Roche, Basel ...
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bioRxiv - Neuroscience 2023Quote: ... with complete Protease inhibitor EDTA-free mix (Roche/SIGMA)) using a Dounce homogenizer to obtain homogenized tissue samples ...
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bioRxiv - Pathology 2023Quote: ... The RNA-seq libraries were prepared with KAPA Stranded mRNA-Seq Illumina® Platforms Kit (Roche) following the manufactureŕs recommendations starting with 500 ng of total RNA as the input material ...