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Citations for Merck :
1 - 50 of 261 citations for hsa mir 222 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Primers for reverse-transcription PCR (RT-PCR) and quantitative RT-PCR (qRT-PCR) were supplied by Merck with standard desalting at 100 µM in TE buffer ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and 1.8 ul of LD forward primer set pools (10 uM per primer, provided by MERCK) using 25 ul of Q5 Hot Start Master Mix (2X ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and 1 ul of FR1 forward primer set pools (10 uM per primer, provided by MERCK) using 25 ul of Q5 Hot Start Master Mix (2X ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% HSA (Merck Millipore, 823022), 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... DNA oligonucleotides (RT-qPCR primers) were obtained from Merck (Rehovot, Israel).
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bioRxiv - Developmental Biology 2023Quote: ... except for a different set of SYBR green primers (Sigma Merck, Supplementary Table 2). The obtained Ct values were analyzed using the ddCt method for relative quantification of mRNA expression derived from three independent experiments ...
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bioRxiv - Cancer Biology 2022Quote: ... Phophorylated MEK was detected using anti-phospho MEK 1/2 (residues 218/222 and 222/226, MERCK, catalogue No ...
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bioRxiv - Developmental Biology 2020Quote: ... and anaesthetised with MS-222 (Sigma-Aldrich/Merck) if required (Alexandre et al. ...
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bioRxiv - Physiology 2019Quote: ... human tubal fluid (HTF) medium and human serum albumin (HSA) (Merck Millipore Corporation ...
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bioRxiv - Immunology 2023Quote: Carrier proteins (HEL, OVA, HSA, BSA and MSA) were purchased commercially (Merck) and dissolved in 0.1 M NaCO3 (pH = 8 ...
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bioRxiv - Developmental Biology 2020Quote: ... anaesthetised with MS-222 (Sigma-Aldrich/Merck, St. Louis, U.S.A.) and fixed in 4% PFA overnight at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... was amplified via PCR with the restriction sites 5′-BamHI/XhoI-3′ (Fw Primer: ATATGGATCCATGTTCGTGTTCCTGGTTCTT; Rv Primer: AATATGAGCAGTACATAAAATGGCCCCTCGAGATAT; purchased from Merck). As vector system ...
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bioRxiv - Developmental Biology 2023Quote: ... The digestion was terminated by α-MEM media with 10% HSA (Merck Millipore, 823022). After digestion ...
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bioRxiv - Bioengineering 2023Quote: ... 2 dpf embryos were manually dechorionated and anesthetized with MS-222 (Merck). 4 nl of the sample were delivered into the common cardinal vein using microcapillaries (TW100-4 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... namely using 1 g/L MS-222 (Ethyl 3-aminobenzoate methanesulfonate, Merck #E10521) and subsequent exsanguination by cutting the gill arches ...
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bioRxiv - Physiology 2022Quote: ... Mild anesthetic induction by MS-222 (50 mg/L, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) [74] was used to treat five masu salmon and two cherry salmon until there was minimal gill movement ...
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bioRxiv - Cell Biology 2023Quote: ... larvae were exposed to 0.1 mg/ml tricaine anesthesia (MS-222, #E10521, Merck, Darmstadt, Germany) and positioned laterally in a 96-well plate (Greiner Bio-One ...
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bioRxiv - Synthetic Biology 2020Quote: ... Gene insertion was verified by PCR using the primer pairs pETUpstream/DuetDOWN or DuetUP2/T7 Terminator (Merck) with Enc or mSOG-containing plasmids as template DNA ...
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bioRxiv - Developmental Biology 2020Quote: ... phosphatase inhibitor cocktail sets II (Merck) and IV (Merck) ...
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bioRxiv - Immunology 2020Quote: ... All primers (Merck) were designed to span exon-exon boundaries and are available upon request ...
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bioRxiv - Neuroscience 2023Quote: ... Primers (Merck, Israel) for mRNA and lncRNA were designed using the NCBI Primer-Blast software ...
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bioRxiv - Biochemistry 2021Quote: ... These Wild Type or Mutant Type let-7a-5p plasmids were co□transfected into treated human chondrocytes cells along with NC mimics or KCNQ1OT1 mimic (Sigma□Aldrich; Merck KGaA) using Lipofectamine 6000 following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... by submersion in 160 mg/l MS-222 (ethyl 3-aminobenzoate methanesulfonate; Sigma-Aldrich, Merck, Darmstadt, Germany) dissolved in tank water ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with protease inhibitor cocktail set III (Cat No. 539134-1 set, 1:100, Merck Millipore, Rahway, NJ). The lysate was centrifuged at 16,000 g at 4 LJ for 10 minutes to remove insoluble samples ...
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bioRxiv - Biochemistry 2021Quote: ... protease inhibitor cocktail set III (Merck Millipore), 1 mM PMSF) ...
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bioRxiv - Biochemistry 2021Quote: ... protease inhibitor cocktail set III (Merck Millipore), 1mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Plant Biology 2020Quote: ... 1X Set III protease cocktail (Merck, 539134), 1 mM NaF ...
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bioRxiv - Molecular Biology 2019Quote: ... Protease Inhibitor Cocktail Set III (Merck Millipore) and SUPERase• In RNase Inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... The sequence-specific primers used are shown in Table S3 (GAPDH and β2M primers were from Eurofins Scientific; other primers were from Merck). The expression levels were normalized to GAPDH and β2M endogenous gene levels ...
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bioRxiv - Molecular Biology 2019Quote: ... using the primers (Merck) listed in Supplementary table 5 ...
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bioRxiv - Microbiology 2021Quote: ... the pUL21 gene was amplified from virus stock by PCR with the oligonucleotide primers 5′-ATGGAGCTTAGCTACGCCAC-3′ and 5′-TTTATTGGGGTCTTTTACACAGACTGTC-3′ using KOD polymerase (Merck) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR amplification of the Round 1 PCR product (1 ul) with 1 ul of JH reverse primer (10 uM, provided by MERCK) and 1 ul of FR1 forward primer set pools (10 uM per primer ...
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bioRxiv - Cell Biology 2023Quote: ... The allele-specific forward primers and common reverse primers were synthesized by Merck https://www.merckgroup.com/ ...
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bioRxiv - Molecular Biology 2023Quote: ... The allele-specific forward primers and common reverse primers were synthesized by Merck https://www.merckgroup.com/ ...
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bioRxiv - Developmental Biology 2022Quote: ... phosphatase inhibitor cocktail sets II and IV (Merck), and phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Cell Biology 2020Quote: E(y)2 was PCR-amplified using primers 5’ - tttggatccccggaattcccgacgatgag-3’ and 5’-tttgcggccgcttaggattcgtcctctggc-3’ and subcloned into pET32a(+) vector (Merck Biosciences) in frame with 6xHis tag using restriction sites BamHI and NotI
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bioRxiv - Cell Biology 2020Quote: ... 1-524) was PCR-amplified using primers 5’ - tttcatatgggtgaagtcaagtccgtg −3’ and 5’-tttctcgagcatgtggaaatgcagttcccg −3’ and subcloned into pET32a(+) vector (Merck Biosciences) in frame with 6xHis tag using restriction sites NdeI and XhoI.
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bioRxiv - Cell Biology 2020Quote: ... 468-1096) was PCR-amplified using primers 5’-tttggtaccgggccctggctgtgcctg-3’ and 5’-tttctcgagtgcggccgcagatcttag-3’ and subcloned into pET32a(+) vector (Merck Biosciences) in frame with 6xHis tag using restriction sites KpnI and XhoI.
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bioRxiv - Synthetic Biology 2020Quote: ... Primers are ordered from Merck Sigma Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... mix with the primers (Merck) listed in Supplementary table 5 ...
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bioRxiv - Biochemistry 2023Quote: ... Primers were obtained from Merck or Eurofins Genomics ...
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bioRxiv - Evolutionary Biology 2020Quote: Larvae were raised as described above until 11 dpf and deeply anesthetized with 160 mg/l of Tricaine (Ethyl 3-aminobenzoate methanesulfonate salt, MS-222, Merck) before dissecting their pectoral fins ...
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bioRxiv - Developmental Biology 2022Quote: Zebrafish and medaka samples from different developmental stages harbouring mutations in vsx genes were deeply anesthetized for 5-10 minutes with 160 mg/L of tricaine (ethyl 3-aminobenzoate methanesulfonate salt; MS-222; Merck) before dissecting their heads ...
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bioRxiv - Biochemistry 2023Quote: ... These treated fishes were anesthetized by immersing the animals in 0.015 μM anesthesia tricaine mesylate (MS 222) (Merck KGaA, USA) for CFU test and fluorescence images capturing.
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with Protease Inhibitor Cocktail Set III (Merck Millipore), mixed by rotation at 4°C for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... complemented with protease inhibitor cocktail set III (Merck Millipore) and phosphatase inhibitor cocktail II (AG Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... or Phosphatase Inhibitor Cocktail Set III (Merck, 524627-1ML). The samples were lysed at 100°C for 4 min ...
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bioRxiv - Neuroscience 2023Quote: ... and Phosphatase Inhibitor Cocktail Set V (Merck Millipore, 524629). The samples were pipetted 10-20 times up and down until dissolved and were incubated on ice for 20 min ...
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bioRxiv - Molecular Biology 2023Quote: FLAG-tagged or HA-tagged cDNA fragment of DDX43 was amplified by RT-PCR from total RNA of silkworm ovaries and cloned into pIEx-1 vector (Merck Millipore/Novagen) or pIExZ vector by In-fusion cloning kit (Takara).
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bioRxiv - Systems Biology 2020Quote: ... supplied with 1% Protease Inhibitor Cocktail Set III (Merck Chemicals). After an incubation of 30 min at 4 °C ...