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Citations for Merck :
1 - 50 of 180 citations for West Nile virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... and Nile Red (Merck) as described previously (Luginbuehl et al. ...
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bioRxiv - Biophysics 2022Quote: ... Nile red was procured from Merck-Sigma Aldrich (India) ...
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bioRxiv - Cell Biology 2020Quote: ... and Nile red (N3013-100MG, Merck, Darmstadt, Germany) were added to a final concentration of 0.4 μg/mL (1 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... the virus-containing supernatant (8ml) was filtered (Steriflip; Merck) before being concentrated 20x (14000 rpm ...
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bioRxiv - Biophysics 2021Quote: ... Freshly excited slices were incubated for 30 min at 37°C in a solution of Nile Red (Cat. No. 72485-100MG, Sigma-Aldrich/Merck) pre-dissolved in methanol at a concentration of 1 mg/ml and diluted 1:20 in PBS ...
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bioRxiv - Immunology 2022Quote: ... or Super-Signal West Pico Chemiluminescent Substrate (Merck-Millipore). The most sensitive reagent ...
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bioRxiv - Molecular Biology 2020Quote: ... lysates (including phosphatase inhibitors (Merck) in the case of the quantitative γH2A-assay ...
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bioRxiv - Cell Biology 2021Quote: ... The virus was concentrated in a 100KDa Amicon centrifugation unit (Merck) at 5000g for 15 mins to achieve a 10-fold concentration ...
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bioRxiv - Neuroscience 2021Quote: ... This concentrated virus solution was filtered (0.22 μm Millex, Merck Millipore) and stored at 4 °C until further use ...
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bioRxiv - Evolutionary Biology 2023Quote: ... virus samples were mixed with SYBR Green I stain (Merck, Germany) and Tris-EDTA buffer in a PCR plate ...
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bioRxiv - Cancer Biology 2023Quote: ... Peroxidase activity was detected using Super-Signal West Pico reagents (Merck) using a CCD camera LAS-4000 (FujiFilm) ...
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bioRxiv - Microbiology 2020Quote: ... virus-containing supernatants were filtered through a 0.45 μm membrane (Merck Millipore). The amount of virus in the supernatants was quantified by RT assay ...
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bioRxiv - Cell Biology 2022Quote: ... Virus amplification was performed in Sf9 TriEX cells (Novagen / Merck #71023-3) grown in TriEx media (Novagen / Merck #71022) ...
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bioRxiv - Genetics 2022Quote: ... Virus was concentrated using Amcion Ultra-15 30k centrifugal filter (Merck#C7715) and centrifugation for 30 min at 3,900rpm.
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bioRxiv - Genomics 2024Quote: ... virus-containing supernatant was filtered through a 0.45 μm filter (Merck Millipore), aliquoted ...
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bioRxiv - Microbiology 2022Quote: ... A PgVV-only lysate was obtained by 0.2 µm filtering of a mixed lysate (Millex, SLGV033RS, Merck Millipore), TFF concentration (Repligen N06-E100-05-N ...
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bioRxiv - Molecular Biology 2021Quote: ... protein lysates were reduced with dithiothreitol (Merck) and cysteine residues were alkylated with iodoacetamide (Merck) ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated using Amicon Ultra-4 centrifugal filter units (Merck-Millipore) and the solution was filtered with a 0.2 µm Acrodisc syringe filter (Sigma-Aldrich).
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bioRxiv - Physiology 2021Quote: ... The virus was further purified using Sepharose G100 SF resin (Merck, Darmstadt, Germany) in Econopac colums (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... Virus was concentrated in PBS using Amicon Ultra-15 Centrifugal Filter Units (Merck) and stored at 4°C.31,77,78 Viral titre was quantified by inverted terminal repeat probe quantitative polymerase chain reaction (PCR) ...
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bioRxiv - Biochemistry 2023Quote: ... Virus- containing supernatant P1 was sterile-filtered using a 0.22 μm filter (Merck). To amplify the virus 1.5 mL P1 was added to 250 mL of Sf21 shaking cell culture at a density of 0.4*106 cells/mL ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μl AAV virus was mixed with 1 μl 20% mannitol (MERCK K93152782 111). The virus and mannitol mixture were injected into a pulled-glass pipette (Warner Instruments ...
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bioRxiv - Biophysics 2023Quote: ... concentrated virus particles were freshly thawed and diluted in phosphate-buffered saline (PBS, Merck, 806552 ...
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bioRxiv - Cell Biology 2024Quote: ... of cell lysates by incubating lysates with 0.5-2.5 µg of antibodies and 20 µl of protein G–Sepharose (Merck, GE17-0618-01) at 4°C for 2 h ...
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bioRxiv - Genomics 2020Quote: ... An amount of 35 or 40 μg whole cell lysates or tissue lysates were resolved by 10% SDS-PAGE and transferred onto PVDF membranes (Merck Millipore, Germany). After incubation in 5% non-fat milk for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Virus-infected culture was mixed with an equal volume of 0.4% Gelzan™ CM (Merck) and spread onto pre-warmed 0.7% Gelzan™ CM/SCV plates ...
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bioRxiv - Microbiology 2020Quote: ... virus-containing medium from producing cells was treated with 15 U/ml DNase I (Merck) and 10 mM MgCl2 for 5 h at 37°C prior to ultracentrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was added to RPE1-hTERT cells together with 8µg/ml polybrene (Merck Life science). Cells were selected using 5 µg/ml puromycin (Santa Cruz ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing cell supernatants were concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore) and exchanged into TexMACS media (Miltenyi Biotec).
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bioRxiv - Microbiology 2023Quote: ... virus stocks were concentrated on a 100 KDa cellulose membrane (Amicon Ultra-15, Merck Millipore) and rinsed three times successively with PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... virus at the MOI of 50 was applied with 8 μg/ml Polybrene (Merck Millipore). Virus-containing medium was replaced with medium containing puromycin (3.5 μg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclear lysates were treated with Benzonase (Merck-Millipore, 70664). Nuclear lysates were pre-cleared on 25 μL Dynabeads M-280 Sheep anti-mouse or antirabbit IgG (Thermo ...
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bioRxiv - Cancer Biology 2023Quote: Protein lysates were prepared using RIPA buffer (Merck/Millipore). SDS-PAGE and western blotting were performed using standard protocols ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The virus supernatants were directly overlaid on H1299 cells in the presence of polybrene (Merck, Germany) at a final concentration of 4 μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... 10mL of virus was concentrated using centrifugation through Amicon® Ultra-15 Centrifugal Filter Units (Merck). Efficacy against steel was assessed at room temperature and 4°C ...
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bioRxiv - Immunology 2022Quote: ... Protein concentrations of lysates were determined using BCA assay (Merck). Proteins in culture supernatants were precipitated using methanol/chloroform ...
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bioRxiv - Cancer Biology 2019Quote: ... The virus was harvested 48 hours post transfection and filtered through a 0.45 μm PES filter (Merck-Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... Respiratory syncytial virus RSV-G was immunoblotted with commercially available goat polyclonal anti-RSV serum (#AB1128, Merck). Coxsackievirus VP1 was immunoblotted with commercially available mouse monoclonal anti-VP1 (3A8 ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... virus was also concentrated using Amicon Ultra-15 Centrifugal Filter Units with 100 kDa cutoff (Merck Millipore). Mice were injected s.c ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 mL of virus supernatant is concentrated using Amicon Ultra-4 30K centrifugal filters (Merck, cat# UFC803024). For transduction of suspension cells ...
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bioRxiv - Neuroscience 2019Quote: ... the cell lysates were treated with benzonase nuclease (Merck, Darmstadt, Germany) at 37°C for 30 min ...
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bioRxiv - Neuroscience 2019Quote: ... the cell lysates were treated with benzonase nuclease (Merck, Darmstadt, Germany) at 37°C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were supplemented with 500 U Recombinant DNAse (Merck 4716728001) and 100 mM PMSF ...
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bioRxiv - Neuroscience 2021Quote: ... The cell lysate was treated with Benzonase (75 U/μl; Merck) for 1h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The phage lysate was treated with DNase-I (Merck cat #11284932001) added to a final concentration of 20 μg/ml and incubated at 37°C for 1 hour to remove bacterial DNA ...
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bioRxiv - Cell Biology 2023Quote: ... cell lysates were precipitated on magnetic carboxylate modified beads (Merck, GE45152105050250) in the presence of 77% (volv/vol ...
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bioRxiv - Developmental Biology 2023Quote: ... the lysate was digested with proteinase K (Merck, Kenilworth, NJ, USA) at 55°C for 2 h ...
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bioRxiv - Biochemistry 2023Quote: ... the lysate was filtrated in Centricon™ 10 kDa (Merck Millipore). The flow-through was loaded into SPE-C18 (MacroSpin Columns ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysate was mixed at 1:100 (v:v) with Benzonase (Merck) to digest precipitated chromatin ...
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bioRxiv - Neuroscience 2020Quote: ... a short protein sequence expressed in the injected virus (1:400 rabbit anti P2A; ABS31, Merck, Darmstadt, Germany). In the third step ...