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Citations for Merck :
1 - 50 of 501 citations for VEGF D Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Human embryonic kidney (HEK293) cells were cultured in Dulbeccoʹs Modified Eagleʹs Medium (DMEM, Merck) containing 10% Fetal Calf Serum and 1% Antibiotic Antimycotic Solution (Sigma Aldrich) ...
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bioRxiv - Genomics 2020Quote: ... was incubated with/without 0.4μg human 6X-His-CDK9/Cyclin T1 (Merck, 140685) and/or 0.4μg human GST-tagged PP2A-α (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... R&D, AF2408), Goat-Anti Human SOX17 (1:100, R&D, AF1924) or Mouse Anti-Human Beta-Tubulin (1:1000, Merck, T8660) primary Chicken Anti-Human MAP2 (1:2000 ...
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bioRxiv - Cancer Biology 2020Quote: ... When stained for human nuclei (MAB1281, Merck Millipore, Fig. 4C-D), this was performed for 30 min at RT in the dark after EdU staining (Click-iT Assay ...
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bioRxiv - Molecular Biology 2022Quote: ... or anti-His (Merck) for His-G5845–1508) ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Synthetic Biology 2021Quote: HEK293 and NIH-3T3 cells were cultured in DMEM (Merck) supplemented with 10% FBS (Merck) ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... whereas HEK293 cells were cultured in DMEM (D5796 MERCK, Darmstadt, Germany) containing 10% (vol./vol. ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Merck) supplemented with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Microbiology 2023Quote: ... and VEGF content was quantified using a custom-made Milliplex Mouse Cytokine/Chemokine Panel (Merck Millipore, USA), a magnetic bead-based multiplex immunoassay following the manufacturer instructions ...
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bioRxiv - Genomics 2023Quote: ... spermatozoa from JF1/MsJ males were capacitated in Human Tubal Fluid medium (Merck Millipore, Cat#MR-070-D) supplemented with 10 mg ml−1 Albumin (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% HI-FBS (Merck/Biochrom), 2 mM L-glutamine (Merck/Biochrom) ...
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bioRxiv - Systems Biology 2023Quote: ... Primary his tag antibody (05-949, Merck), stored in a skim milk solution ...
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bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
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bioRxiv - Immunology 2020Quote: ... Plasma levels of vascular endothelial growth factor (VEGF) was measured using the Milliplex map kit system by Merck Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were incubated with 50 µM 17ODYA (17-octadecynoic acid, Merck) added from stock solution in DMSO or with 0.05% DMSO in DMEM containing 2% charcoal-stripped FBS and 30 mM HEPES for 4 h at 37°C ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: HEK293 cells were cultured in Dulbecco⍰s Modified Eagle⍰s Medium (DMEM, Merck) containing 10% Fetal Calf Serum and 1% Antibiotic Antimycotic Solution (Sigma Aldrich) ...
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Biochemistry 2024Quote: ... we utilized the His-Bind purification kit (Merck, USA) followed by manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells seeded in 6-well plates or 100mm dishes were transfected using GeneJuice (Merck) and 1 to 3µg DNA ...
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bioRxiv - Neuroscience 2024Quote: ... we used fixed HEK293 cells labeled with primary antibody (rabbit anti-GM130, 1:1000, Merck) and secondary antibody (goat anti-rabbit conjugated with Alexa Fluor 555 ...
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bioRxiv - Cancer Biology 2022Quote: ... and VEGF (Cat. No HCYTOMAG-60K) were quantified using Milliplex MAP Luminex microbead assays according to the manufacturer’s instructions (Merck Millipore, Darmstadt, DE). Samples were analyzed without dilution in duplicate ...
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was added to a His Trap HP column (Merck) previously equilibrated with buffer A ...
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bioRxiv - Cell Biology 2021Quote: ... previously coupled to 1 ml HI-TRAP NHS columns (GE/Merck) packed with Sepharose pre-activated for covalent coupling of ligands containing primary amino groups ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Merck). Immortalized human C20 microglia were kindly provided by David Alvarez-Carbonell (21) ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Cell Biology 2019Quote: ... and subsequently with 5IU of human chorionic gonadotropin (hCG) (Intervet, GesmbH) were mated and zygotes were isolated in M2 media (Merck Millipore, MR-015P-D) and cultured in KSOM medium (Cosmo Bio Co. ...
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bioRxiv - Bioengineering 2024Quote: ... Unlabeled AAVs were detected on HEK293 cells post fixation by anti-AAV9 clone HL2372 (Merck, MABF2309-100UL) at a 1:500 dilution followed by AF647-labeled anti mIgG H+L.
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bioRxiv - Microbiology 2021Quote: ... actinomycin D (A1410, Merck), and cycloheximide (037-20991 ...
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bioRxiv - Cell Biology 2022Quote: ... Cytochalasin D (Merck, #C8273), Nocodazole (Merck ...
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bioRxiv - Biochemistry 2021Quote: ... 0.6% D-glucose (Merck), 1 mM sodium pyruvate (Nacalai Tesque ...
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bioRxiv - Physiology 2020Quote: D-Glucose (Merck, 8342) was dissolved in ddH2O and supplemented to the NGM after autoclaving and UVB killed E ...
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bioRxiv - Cell Biology 2023Quote: ... dihydroxytestosterone (Merck, #D-073) or EtOH as solvent control ...
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bioRxiv - Cell Biology 2023Quote: Cytochalasin D (Merck C2618) was used at concentrations ranging from 100nM to 10 µM ...
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bioRxiv - Developmental Biology 2022Quote: Ciliobrevin D (Merck, 250401) 5 mM dimethyl sulfoxide (DMSO ...
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bioRxiv - Bioengineering 2023Quote: ... Cytochalasin D (C8273, Merck); CA3 (CIL56 ...
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bioRxiv - Cell Biology 2024Quote: ... D-biotin (B4501; Merck) at a final concentration of 500 µM was added to induce the RUSH.
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a His-bind resin column (Merck Millipore) (1 ml ...
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bioRxiv - Plant Biology 2023Quote: ... and purified with Ni-NTA His•Bind® Resin agarose (Merck, 70666). The binding reaction was performed in 20 µL of binding buffer [10 mM Tris (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... His tagged proteins were labeled using Ni-NTA-ATTO 488 (Merck Group) at a ratio of ATTO/protein = 1/500 in MN buffer (50 mM MES pH 6 ...
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bioRxiv - Biochemistry 2019Quote: ... 5 was performed using 600 μL HEK293 cells (±CL) previously treated with 2000 U/mL Benzonase (Merck, 70664) in the recommended buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: hTERT RPE-1 and HEK293(T) cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM/F12; Merck) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Physiology 2019Quote: ... human tubal fluid (HTF) medium and human serum albumin (HSA) (Merck Millipore Corporation ...
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bioRxiv - Plant Biology 2020Quote: ... His-tagged proteins were washed and purified using the Amicon Pro System (Merck) according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: ... Cytochalasin D (Merck Millipore, #250255), Jasplakinolide (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... Cytochalasin D (1 µM, Merck) and Y-27632 (30 µM ...
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bioRxiv - Neuroscience 2023Quote: ... D-tubocurarin (Merck-Sigma-Aldrich) was used at a concentration of 1 mM in ASW ...