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Citations for Merck :
1 - 50 of 477 citations for Siglec 8 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... HEK 293 cells were seeded in culture plates (Corning, Merck, Darmstadt, Germany) and incubated in DMEM (PAN Biotech ...
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bioRxiv - Neuroscience 2021Quote: HEK 293 cells were seeded in cell culture plates (Corning, Merck, Darmstadt, Germany) in the day before at the appropriate density ...
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bioRxiv - Cell Biology 2023Quote: HEK cells were grown in an 8-well chamber slide (Millicell EZ slide, Merck-Millipore, Burlington, MA) to early confluence ...
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bioRxiv - Genomics 2020Quote: ... was incubated with/without 0.4μg human 6X-His-CDK9/Cyclin T1 (Merck, 140685) and/or 0.4μg human GST-tagged PP2A-α (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... or anti-His (Merck) for His-G5845–1508) ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were sub-cultured on days 4 and 8 of differentiation onto human fibronectin (Merck Millipore, #FC010) coated plates at a ratio of 1:4 ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Immunology 2023Quote: ... using the 293-free transfection reagent (Merck, Darmstadt, Germany) according to the manufactures’ protocols ...
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bioRxiv - Biochemistry 2023Quote: ... the Siglec-7-Spy Tag-TEV-Fc was concentrated using an AMICON (Molecular Weight Cut Off 30 kDa – Merck Millipore) to approximately 0.15 mg/ml measured via NanoDrop as described above ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: 293 cells were seeded in culture plates (Corning, Merck, Darmstadt, Germany) and incubated overnight at 37°C with 5% CO2 in DMEM (PAN Biotech ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% HI-FBS (Merck/Biochrom), 2 mM L-glutamine (Merck/Biochrom) ...
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bioRxiv - Systems Biology 2023Quote: ... Primary his tag antibody (05-949, Merck), stored in a skim milk solution ...
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bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
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bioRxiv - Cancer Biology 2021Quote: ... 8) eosin (Merck. #1.15935.0100) for 1 min ...
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bioRxiv - Cell Biology 2021Quote: ... 8 mM MgCl2 (Merck), 40 mM EGTA (Sigma)) ...
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bioRxiv - Cell Biology 2021Quote: ... 8 mM MgCl2 (Merck), 40 mM EGTA (Sigma)) ...
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bioRxiv - Cell Biology 2023Quote: ... CCK-8 (96992, Merck) was used according to the manufacturer’s instructions and measurements were performed at the indicated time points ...
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bioRxiv - Immunology 2023Quote: ... 8 mM MgCl2 (Merck), 320 mM sucrose ...
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Biochemistry 2024Quote: ... we utilized the His-Bind purification kit (Merck, USA) followed by manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... HEK 293T cells grown on 15-cm dishes were transfected using polyethylenimine (PEI) (Merck), a transfection reagent for standard triple transfection protocol ...
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bioRxiv - Cell Biology 2022Quote: ... JNK-I-8 (Merck SML1246), SB-203580 (MedKoo ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-8-oxoguanine (MAB3560, Merck), anti-phospho-histone H2AX (Ser 139 ...
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Tracer-based metabolomics for profiling nitric oxide metabolites in a 3D microvessel-on-a-chip modelbioRxiv - Cell Biology 2023Quote: ... BH4 (Merck, 69056-38-8). RPMI SILAC - medium deficient in Arginine was obtained from (Thermofisher ...
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bioRxiv - Molecular Biology 2024Quote: ... 8 µL proteinase K (10 mg/mL) and 8 µL of 1 M DTT (Merck) was added to the sperm fraction which were vortexed and incubated at 56 °C for 60 min (mock casework samples ...
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was added to a His Trap HP column (Merck) previously equilibrated with buffer A ...
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bioRxiv - Cell Biology 2021Quote: ... previously coupled to 1 ml HI-TRAP NHS columns (GE/Merck) packed with Sepharose pre-activated for covalent coupling of ligands containing primary amino groups ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Merck). Immortalized human C20 microglia were kindly provided by David Alvarez-Carbonell (21) ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... 50 mM Tris pH 8 (Merck), 1 % IGEPAL® CA-630 (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 mM Tris pH 8 (Merck), 1 % v/v IGEPAL CA-630 (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... 8 μg/ml RNase A (Merck) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... cell counting kit 8 (Merck, 96992) was applied for 2.5 h as recommended by the manufacturer.
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bioRxiv - Cell Biology 2023Quote: ... HeLaM and HEK-293T cells were grown in Dulbeccos modified Eagles medium (DMEM) (Merck, D6429-500ML) supplemented with 10% Foetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a His-bind resin column (Merck Millipore) (1 ml ...
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bioRxiv - Plant Biology 2023Quote: ... and purified with Ni-NTA His•Bind® Resin agarose (Merck, 70666). The binding reaction was performed in 20 µL of binding buffer [10 mM Tris (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... His tagged proteins were labeled using Ni-NTA-ATTO 488 (Merck Group) at a ratio of ATTO/protein = 1/500 in MN buffer (50 mM MES pH 6 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells (8 × 104) were seeded on fibronectin (bovine, 0.1 mg/mL) coated Millicell EZ SLIDE 8-Well (Merck) in 300 µL media/well (RPMI + 10% FCS + 1% ultraglutamine + 1% Pen/Strep + 1% HEPES + 1% Na-pyruvate + 1% MEM NEAA + 0.1% mercaptoethanol).
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bioRxiv - Physiology 2023Quote: ... phloretin (50 mg/L; n=8) or ritonavir (60 mg/L; n=8) (all from Merck, Darmstadt, Germany) in 20 mL of 2.3 % glucose peritoneal dialysis fluid (Balance ...
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bioRxiv - Bioengineering 2023Quote: ... 8 μg/mL Calcein AM (Merck, Germany), 4 μg/mL PI (Merck ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.001% phenol red (Merck, 143-74-8), pH 7.8) ...
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bioRxiv - Physiology 2019Quote: ... human tubal fluid (HTF) medium and human serum albumin (HSA) (Merck Millipore Corporation ...
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bioRxiv - Plant Biology 2020Quote: ... His-tagged proteins were washed and purified using the Amicon Pro System (Merck) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Human HDL (Merck Millipore) was incubated with NS1 for 1 hour at 37°C prior to the DSC experiments ...
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bioRxiv - Immunology 2021Quote: Native human insulin (Merck) was pre-diluted in water to 1 mg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... pSmad1/5/8 (Merck, AB3848-I, 1/200), Ki67 (Cell Signaling Technology ...