Labshake search
Citations for Merck :
1 - 50 of 1370 citations for Siglec 5 Human HEK293 His Flag Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... the Siglec-7-Spy Tag-TEV-Fc was concentrated using an AMICON (Molecular Weight Cut Off 30 kDa – Merck Millipore) to approximately 0.15 mg/ml measured via NanoDrop as described above ...
-
bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
-
bioRxiv - Immunology 2020Quote: ... FITC conjugated goat anti-human IgG Fc was from Merck, Dorset ...
-
bioRxiv - Cell Biology 2024Quote: Human embryonic kidney (HEK293) cells were cultured in Dulbeccoʹs Modified Eagleʹs Medium (DMEM, Merck) containing 10% Fetal Calf Serum and 1% Antibiotic Antimycotic Solution (Sigma Aldrich) ...
-
bioRxiv - Genomics 2020Quote: ... was incubated with/without 0.4μg human 6X-His-CDK9/Cyclin T1 (Merck, 140685) and/or 0.4μg human GST-tagged PP2A-α (Sigma ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5 μg/ml human insulin (Merck), and 50 μM hydrocortisone (Cayman ...
-
bioRxiv - Microbiology 2021Quote: ... and the supernatant was incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
-
bioRxiv - Molecular Biology 2021Quote: ... and goat anti-Human IgG (Fc specific)-Cy3 (1:1000 dilution in binding buffer; Merck). Finally ...
-
bioRxiv - Biochemistry 2019Quote: ... 5 was performed using 600 μL HEK293 cells (±CL) previously treated with 2000 U/mL Benzonase (Merck, 70664) in the recommended buffer (50 mM Tris ...
-
bioRxiv - Microbiology 2021Quote: ... The binding of CoVHH1-FLAG to S1 subunits-His tag was detected by incubating for 1 hour at room temperature with mouse monoclonal ANTI-FLAG® M2-Peroxidase (Merck) diluted with PBST ...
-
bioRxiv - Cell Biology 2020Quote: Flp-In™T-REx™293 and HEK293 cell lines were grown under standard conditions (37°C, 5% CO2) in Dulbecco’s modified Eagle’s medium (Merck-Sigma-Aldrich, D5796). For Flp-In™T-REx™293 the medium was supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Molecular Biology 2022Quote: ... or anti-His (Merck) for His-G5845–1508) ...
-
bioRxiv - Cancer Biology 2023Quote: ... T cell medium (human serum was replaced with FCS during thawing) and incubated for 15 minutes with 25U/mL benzonase (Merck). After washing ...
-
bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
-
bioRxiv - Cell Biology 2019Quote: ... FLAG (mouse; Merck), FLAG (rat ...
-
bioRxiv - Cancer Biology 2023Quote: ... FLAG (Merck F1804), TUBULIN (Santa Cruz ...
-
bioRxiv - Cell Biology 2023Quote: ... FLAG tag (Merck, Darmstadt ...
-
bioRxiv - Cell Biology 2024Quote: ... Flag (MERCK, F3165), GAPDH (Genetex ...
-
bioRxiv - Synthetic Biology 2021Quote: HEK293 and NIH-3T3 cells were cultured in DMEM (Merck) supplemented with 10% FBS (Merck) ...
-
bioRxiv - Microbiology 2021Quote: ... The sonicated cellular extract was spun down and the supernatant has been incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
-
bioRxiv - Immunology 2019Quote: ... anti-human C5aR or nonpeptide C5aR antagonist W-54011 (5 μM, Merck Millipore)(25 ...
-
bioRxiv - Immunology 2019Quote: ... Fc IgG (AG714, Merck Millipore) at 2 μg/ml in 150 μl of PBS for one hour ...
-
bioRxiv - Biophysics 2019Quote: ... supplemented with 10 % FCS (Merck), 100 µg ml−1 penicillin (Life technologies) ...
-
bioRxiv - Biophysics 2019Quote: ... supplemented with 10% FCS (Merck), 100 µg ml−1 penicillin (Life technologies) ...
-
bioRxiv - Biophysics 2020Quote: ... supplemented with 10% FCS (MERCK), 100 μg ml−1 penicillin (Life technologies) ...
-
bioRxiv - Biochemistry 2021Quote: ... supplemented with 10% FCS (Merck) and 2 mM L-glutamine (Thermo Scientific ...
-
bioRxiv - Developmental Biology 2023Quote: ... + 10% FCS (Merck Millipore S0615) + 1% P/S (Thermo Fisher #15140122) ...
-
bioRxiv - Immunology 2024Quote: ... supplemented with 10% FCS (Merck), 2mM L-Glutamine (Gibco ...
-
bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
-
bioRxiv - Cancer Biology 2022Quote: ... whereas HEK293 cells were cultured in DMEM (D5796 MERCK, Darmstadt, Germany) containing 10% (vol./vol. ...
-
bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Merck) supplemented with 10% FBS and 1% penicillin/streptomycin ...
-
bioRxiv - Cell Biology 2023Quote: ... anti-FLAG immunoprecipitations were performed using anti-FLAG M2 magnetic beads (Merck). All beads were washed 3x in lysis buffer prior to use ...
-
bioRxiv - Genomics 2023Quote: ... anti-FLAG (F1804, Merck), anti-SCC4/MAU2 (ab183033 ...
-
bioRxiv - Developmental Biology 2023Quote: ... injection of 5 IU of human chorionic gonadotrophin (hCG; Pregnyl, Merck, Kilsyth, VIC, Australia) 48 h later.
-
bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% HI-FBS (Merck/Biochrom), 2 mM L-glutamine (Merck/Biochrom) ...
-
bioRxiv - Systems Biology 2023Quote: ... Primary his tag antibody (05-949, Merck), stored in a skim milk solution ...
-
bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
-
bioRxiv - Microbiology 2022Quote: ... and the FLAG-tagged proteins were immunoprecipitated with anti-FLAG agarose (A2220, MERCK). Input samples were collected as described above.
-
bioRxiv - Cell Biology 2023Quote: The anti-Flag primary antibody was purchased from Merck (Darmstadt, Germany: Flag F3165). Secondary antibodies were purchased from Thermo Fisher Scientific.
-
bioRxiv - Molecular Biology 2024Quote: ... FLAG-tagged proteins were detected using M2 anti-FLAG antibody (mouse, F1084, Merck) followed and fluorescent Anti-Mouse Alexa Fluor™ Plus 800 ...
-
bioRxiv - Cell Biology 2020Quote: ... containing 10% FCS (Merck KGA, Germany), 4 mM glutamine (Merck KGA ...
-
bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were incubated with 50 µM 17ODYA (17-octadecynoic acid, Merck) added from stock solution in DMSO or with 0.05% DMSO in DMEM containing 2% charcoal-stripped FBS and 30 mM HEPES for 4 h at 37°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... All human cell lines were cultured at 37℃ in 5% CO2 in DMEM media (Merck) containing 1% antibiotic (Gibco ...
-
bioRxiv - Molecular Biology 2023Quote: ... and anti-FLAG (Merck; F3165).
-
bioRxiv - Neuroscience 2023Quote: ... Flag (Merck, Darmstadt, Germany, F1804), GFP (Merck ...
-
bioRxiv - Molecular Biology 2023Quote: ... Anti-Flag-M2 (Merck, F3165).
-
bioRxiv - Cell Biology 2023Quote: ... FLAG M2 beads (Merck M8823) were used ...
-
A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: HEK293 cells were cultured in Dulbecco⍰s Modified Eagle⍰s Medium (DMEM, Merck) containing 10% Fetal Calf Serum and 1% Antibiotic Antimycotic Solution (Sigma Aldrich) ...
-
bioRxiv - Molecular Biology 2020Quote: U2OS cells were transfected with FLAG-LotB or FLAG-LotC by GeneJuice transfection reagent (Merck) for 24 h and fixed by 4% paraformaldehyde for 20 min ...
-
bioRxiv - Immunology 2020Quote: ... and 10% Fetal calf serum (FCS) (Merck). Both cell lines were cultivated in T75 culture flasks ...