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Citations for Merck :
1 - 50 of 465 citations for SEMA3A Human HEK293 His Flag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Cell Biology 2024Quote: Human embryonic kidney (HEK293) cells were cultured in Dulbeccoʹs Modified Eagleʹs Medium (DMEM, Merck) containing 10% Fetal Calf Serum and 1% Antibiotic Antimycotic Solution (Sigma Aldrich) ...
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bioRxiv - Genomics 2020Quote: ... was incubated with/without 0.4μg human 6X-His-CDK9/Cyclin T1 (Merck, 140685) and/or 0.4μg human GST-tagged PP2A-α (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... The binding of CoVHH1-FLAG to S1 subunits-His tag was detected by incubating for 1 hour at room temperature with mouse monoclonal ANTI-FLAG® M2-Peroxidase (Merck) diluted with PBST ...
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bioRxiv - Molecular Biology 2022Quote: ... or anti-His (Merck) for His-G5845–1508) ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG (mouse; Merck), FLAG (rat ...
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bioRxiv - Cancer Biology 2023Quote: ... FLAG (Merck F1804), TUBULIN (Santa Cruz ...
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bioRxiv - Cell Biology 2023Quote: ... FLAG tag (Merck, Darmstadt ...
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bioRxiv - Cell Biology 2024Quote: ... Flag (MERCK, F3165), GAPDH (Genetex ...
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bioRxiv - Synthetic Biology 2021Quote: HEK293 and NIH-3T3 cells were cultured in DMEM (Merck) supplemented with 10% FBS (Merck) ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... whereas HEK293 cells were cultured in DMEM (D5796 MERCK, Darmstadt, Germany) containing 10% (vol./vol. ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Merck) supplemented with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2023Quote: ... anti-FLAG immunoprecipitations were performed using anti-FLAG M2 magnetic beads (Merck). All beads were washed 3x in lysis buffer prior to use ...
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bioRxiv - Genomics 2023Quote: ... anti-FLAG (F1804, Merck), anti-SCC4/MAU2 (ab183033 ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% HI-FBS (Merck/Biochrom), 2 mM L-glutamine (Merck/Biochrom) ...
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bioRxiv - Systems Biology 2023Quote: ... Primary his tag antibody (05-949, Merck), stored in a skim milk solution ...
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bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
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bioRxiv - Microbiology 2022Quote: ... and the FLAG-tagged proteins were immunoprecipitated with anti-FLAG agarose (A2220, MERCK). Input samples were collected as described above.
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bioRxiv - Cell Biology 2023Quote: The anti-Flag primary antibody was purchased from Merck (Darmstadt, Germany: Flag F3165). Secondary antibodies were purchased from Thermo Fisher Scientific.
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bioRxiv - Molecular Biology 2024Quote: ... FLAG-tagged proteins were detected using M2 anti-FLAG antibody (mouse, F1084, Merck) followed and fluorescent Anti-Mouse Alexa Fluor™ Plus 800 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were incubated with 50 µM 17ODYA (17-octadecynoic acid, Merck) added from stock solution in DMSO or with 0.05% DMSO in DMEM containing 2% charcoal-stripped FBS and 30 mM HEPES for 4 h at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and anti-FLAG (Merck; F3165).
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bioRxiv - Neuroscience 2023Quote: ... Flag (Merck, Darmstadt, Germany, F1804), GFP (Merck ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-Flag-M2 (Merck, F3165).
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bioRxiv - Cell Biology 2023Quote: ... FLAG M2 beads (Merck M8823) were used ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: HEK293 cells were cultured in Dulbecco⍰s Modified Eagle⍰s Medium (DMEM, Merck) containing 10% Fetal Calf Serum and 1% Antibiotic Antimycotic Solution (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2020Quote: U2OS cells were transfected with FLAG-LotB or FLAG-LotC by GeneJuice transfection reagent (Merck) for 24 h and fixed by 4% paraformaldehyde for 20 min ...
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bioRxiv - Biochemistry 2020Quote: ... Flag-tagged proteins were eluted by incubation with 150 μg/mL Flag tag peptide (Merck; F3290) with 0.1% digitonin in solubilisation buffer for 30 min at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Flag pull down was performed incubating cleared cell lysates with Anti-flag M2 affinity gel (Merck) during 2h ...
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bioRxiv - Genetics 2020Quote: ... and incubated with 1:3500 anti-FLAG antibody (monoclonal anti-FLAG M2; Merck KGaA, Darmstadt, Germany).
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Biochemistry 2024Quote: ... we utilized the His-Bind purification kit (Merck, USA) followed by manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-Flag M2 (F1804, Merck), rabbit anti-SOH (07-2139 ...
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bioRxiv - Neuroscience 2021Quote: ... anti-FLAG M2 magnetic beads (Merck) were used ...
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bioRxiv - Plant Biology 2021Quote: ... and anti-FLAG antibodies (Merck Millipore).
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bioRxiv - Plant Biology 2021Quote: ... Anti-Flag (Merck, cat. no. F1804) was used at 1:2000 in 1× PBST solution dissolved with 5% skimmed milk ...
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bioRxiv - Microbiology 2023Quote: ... anti- Flag-M2 (F1804-200UG Merck) 1/1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-FLAG (M2, Merck Millipore/Sigma), anti-DYKDDDDK peroxidase conjugated (Wako) ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-FLAG (Merck, F3165, 1:1000), anti-ELAVL1 (SantaCruz Biotech ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells seeded in 6-well plates or 100mm dishes were transfected using GeneJuice (Merck) and 1 to 3µg DNA ...
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bioRxiv - Neuroscience 2024Quote: ... we used fixed HEK293 cells labeled with primary antibody (rabbit anti-GM130, 1:1000, Merck) and secondary antibody (goat anti-rabbit conjugated with Alexa Fluor 555 ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-FLAG M2 Affinity gel (Sigma-Merck) was equilibrated in lysis buffer and gently shaked at 4°C for 3 hours with the lysate ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-FLAG® M2 Magnetic Beads (Merck) were equilibrated in lysis buffer and incubated with the cell lysate for 1 hours at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... or anti-FLAG magnetic beads (#M8823, Merck). Lysates were incubated at 4°C under agitation overnight ...
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bioRxiv - Plant Biology 2021Quote: ... α-FLAG-HRP (A8592, Merck; 1:5,000) and α-rabbit-HRP (A-0545 ...
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bioRxiv - Immunology 2021Quote: ... using mouse anti-FLAG (monoclonal M2, Merck), rabbit anti-myc (Cell Signaling ...
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bioRxiv - Biochemistry 2023Quote: ... Blots were probed with FLAG (Merck, F3165), ubiquitin (Novus ...
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bioRxiv - Molecular Biology 2023Quote: ... ANTI-FLAG M2 Affinity Gel (Merck, A2220) beads were equilibrated by washing two times with 1× TBS and one time with Co-IP buffer ...