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Citations for Merck :
1 - 50 of 384 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: Phosphorylation of Sam68 N-term and C-term were obtained by incubation with CDK1/Cyclin B (Merck, catalogue 14-450) at a Cdk1:Sam68 ratio of 5:1000 at 20 °C ...
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bioRxiv - Microbiology 2022Quote: ... The SARS CoV-2 glycoproteins were then purified by divalent cation affinity chromatography using TALON resin (Merck) or HiTrap IMAC FF followed by size exclusion chromatography using a Superose 6 Increase 10/300 column linked to an AKTApure instrument (Cytiva) ...
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bioRxiv - Microbiology 2022Quote: ... anti-coronavirus antibody (MAB9013, Merck Millipore), SARS-CoV-2 nucleocapsid antibody (HL344 ...
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bioRxiv - Microbiology 2021Quote: 10His-tagged MA gene was expressed in Escherichia coli (E. coli) strain BL21 (DE3) (Merck, Darmstadt, Germany). The 10His-tagged MA protein was initially purified with nickel-NTA affinity column chromatography followed by TEV cleavage ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Neuroscience 2023Quote: ... MOG (myelin oligodendrocyte glycoprotein, Cat#MAB5622, Merck Millipore) and NFH (neurofilament heavy chain ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The mouse anti-coronavirus monoclonal antibody MAB9013 was purchased from Merck (Darmstadt, Germany). The secondary antibody peroxidase-conjugated AffiniPure F (ab’)2 Fragment Goat Anti-Mouse IgG (H+L ...
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bioRxiv - Microbiology 2024Quote: ... The 1 mL coronavirus suspension was then washed twice using AmiconUltra (Merck Millipore) with 10 mL phosphate buffer ...
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bioRxiv - Microbiology 2023Quote: ... S1 (N5661-50KU, Merck), and Micrococcal nuclease (EN0181 ...
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bioRxiv - Genomics 2022Quote: ... Keratanase (G6920-5UN)) and a glycoprotein deglycosylation kit (Merck catalog #362280). We then used Water-18O (97% atom ...
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bioRxiv - Developmental Biology 2024Quote: ... and 0.1% N,N,N’,N’-Tetramethylethylenediamine (Merck), to make the gel at a stiffness of 100KPa ...
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bioRxiv - Microbiology 2023Quote: N,N,N’,N’-tetrakis-(2-pyridylmethyl)ethylenediamine (TPEN) (Merck, 616394), made up in DMSO and used at 5 µM ...
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bioRxiv - Biochemistry 2019Quote: EcSerRS (from E. coli strain B ER2560) and SaSerRS (from S. aureus seg50 (1150)) were cloned into pET52b(+) vector (Merck Millipore, Germany) using the NcoI and SacI restriction sites allowing for the production of protein with a thrombin cleavable C-terminal His10-tag ...
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bioRxiv - Cell Biology 2024Quote: ... coli (Merck). Cells were grown at 37 °C in lysogen broth medium supplemented with 50 μg .mL−1 kanamycin (and 34 μg.mL−1 of chloramphenicol for Rosetta ...
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bioRxiv - Microbiology 2022Quote: N,N-dimethylethylenediamine (Merck, 95%), 4-chloro-7-nitrobenzofurazan (NBD-Cl ...
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Bioengineering 2022Quote: ... 4 μl of N,N,N’,N’-Tetramethylethylendiamine (TEMED, #612-103-00-3, Sigma-Aldrich now Merck) was added to 1 ml of the monomer solution ...
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bioRxiv - Developmental Biology 2023Quote: ... and KiCqStart SYBR green primers (Sigma Merck, Table S1). Obtained Ct values were used for relative quantification of mRNA expression using the ddCt method ...
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bioRxiv - Biochemistry 2023Quote: ... Silica gel (E, Merck) was used for flash chromatography ...
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Actin binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin IIbioRxiv - Biophysics 2022Quote: ... coli (Merck Millipore), and purified basically in the same way as Rng2CHD ...
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bioRxiv - Immunology 2021Quote: ... dissolved in N,N-dimethylformamide (Merck Millipore) as substrate ...
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bioRxiv - Microbiology 2020Quote: ... was dissolved in N,N-dimethylformamide (Merck Millipore) and used for peroxidase-dependent staining ...
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bioRxiv - Cell Biology 2022Quote: ... 0.15 mg/ml N,N’-Methylenebisacrylamide (Merck, M7279), 11.7 mg/ml NaCl in 1x PBS) ...
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bioRxiv - Biochemistry 2020Quote: ... high ethanol concentration (E. Merck), Xylol ...
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bioRxiv - Biochemistry 2020Quote: ... HRP-conjugated streptavidin (E. Merck), 3.3 ‘diaminobenzidine (DAB) ...
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bioRxiv - Cell Biology 2023Quote: ... anti-E-cadherin (#MABT26, Merck), anti-Paxillin (#GTX125891 ...
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bioRxiv - Microbiology 2020Quote: ... coli ROSETTA strain (Merck) using pE-SUMO as an expression plasmid (86 ...
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bioRxiv - Cell Biology 2021Quote: ... coli BL21 (Novagen/ Merck Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... coli Rosetta2 (DE3) (Merck); TALON Metal Affinity Resin (Clontech) ...
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bioRxiv - Biochemistry 2023Quote: ... coli Rosetta2 (DE3) (Merck) was transformed using the constructed plasmid and cultured at 37°C in LB medium containing 30 μg/ml kanamycin and 34 μg/ml chloramphenicol ...
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bioRxiv - Biochemistry 2024Quote: ... coli C41(DE3) (Merck) co-transformed with pET22-Wscytc and pEC86 (which harbors the ccm genes for production of the machinery for c-type heme biosynthesis and maturation (Arslan et al. ...
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bioRxiv - Biochemistry 2019Quote: ... Bio-Lab Ltd, Jerusalem, Israel) in N,N-dimethylformamide (DMF, Bio-Lab Ltd, Jerusalem, Israel) combined with N,N-Diisopropylethylamine (DIPEA, Merck, Rehovot, Israel) for a 1 h cycle ...
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... E-cadherin (1:300, Merck, #U3254), α-actinin (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... E-Cadherin (1:600, Merck U3254), Glucagon (1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli Rosetta 2 cells (Merck) by induction with 0.2 mM isopropyl β-d-1-thiogalactopyranoside for 18 h at 18 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... coli Rosetta 2 (DE3) (Merck) and purified using Glutathione Sepharose High Performance beads (GE Healthacare ...
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bioRxiv - Cell Biology 2019Quote: ... coli Rosetta2 pLysS (Merck 71403). Overnight cultures were diluted 1:50 in a final volume of 50 ml ...
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bioRxiv - Cell Biology 2019Quote: ... coli Rosetta2 pLysS (Merck 71403) were used ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21 (DE3) cells (Merck) and the mutant proteins were expressed and purified according to the protocol described by Oliver ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Coli BL21 (DE3) cells (Merck) were transformed with the pET28a(+)-scDH and pET28a(+)-ΔscDH and grown in LB medium with 35 μg/ml kanamycin at 37 °C and shaken until an OD600 of ∼ 0.6 ...
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bioRxiv - Molecular Biology 2022Quote: ... coli BL21(DE3) star (Merck). Luria Bertani (LB ...
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bioRxiv - Genetics 2022Quote: ... coli O111:B4 (#LPS25, Merck) or Pam2CSK4 (#tlrl-pm2s-1 ...
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bioRxiv - Immunology 2023Quote: ... coli serotype O55:B5 (Merck) for 24 to 48 hours to arrive at a mature BMDC culture or alternatively frozen to -80°C in 90% FCS (Biowest ...
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bioRxiv - Immunology 2023Quote: ... coli serotype O55:B5 (Merck) for 24 to 48 hours to induce B-cell blast formation.
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bioRxiv - Microbiology 2023Quote: ... coli expression vector (Merck-Novagen) using Gibson assembly ...
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bioRxiv - Microbiology 2021Quote: ... and PCR of the spike was performed using KOD polymerase (Merck). For next generation sequencing RNA from virus-containing samples were extracted using the QIAsymphony DSP Virus/Pathogen mini kit (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... Spike plasmid was transfected with X-tremeGENE HP Transfection Reagent (Merck) into HEK-293T cells ...
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bioRxiv - Microbiology 2021Quote: ... Spike plasmid was transfected with X-tremeGENE HP Transfection Reagent (Merck) into HEK-293T cells ...
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bioRxiv - Microbiology 2021Quote: ... Spike plasmid was transfected with X-tremeGENE HP Transfection Reagent (Merck) into HEK-293T cells ...
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bioRxiv - Microbiology 2022Quote: ... Spike plasmid was transfected with X-tremeGENE HP Transfection Reagent (Merck) into HEK-293T cells ...