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Citations for Merck :
1 - 50 of 1134 citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The SARS CoV-2 glycoproteins were then purified by divalent cation affinity chromatography using TALON resin (Merck) or HiTrap IMAC FF followed by size exclusion chromatography using a Superose 6 Increase 10/300 column linked to an AKTApure instrument (Cytiva) ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Microbiology 2020Quote: ... The following secondary antibodies were used: sheep α-rabbit horseradish peroxidase (HRP) (catalogue number AP510P, 1/10,000; Merck) and goat α-mouse HRP (STAR117P ...
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bioRxiv - Microbiology 2022Quote: ... After testing for SARS-CoV-2 by PCR test (COBAS 6800, Merck México, Mexico city), they were classified as positive (AP ...
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bioRxiv - Plant Biology 2024Quote: ... 20 µg mL-1 horseradish peroxidase (Merck) together with elicitors ...
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bioRxiv - Plant Biology 2021Quote: ... 20 μg mL−1 horseradish peroxidase (P6782, Merck)) with or without elicitors ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and 20 µg/mL horseradish peroxidase (Merck P6782). ROS generation was measured at 0-60 minutes using a High-Resolution Photon Counting System (Photek HRPCS218) ...
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bioRxiv - Microbiology 2022Quote: ... The sections were then incubated overnight in PBS with 0.2% BSA and 0.05% Tween-20 with primary antibodies directed against SARS-CoV-2 Nucleocapsid Protein (1:500; mouse monoclonal, # ZMS1075, Merck); Iba1 (1 ...
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bioRxiv - Microbiology 2022Quote: ... the Milliplex SARS-CoV-2 Antigen Panel 1 IgG was used according to the manufacturer’s instructions (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2022Quote: ... Luminata Forte Western horseradish peroxidase (HRP) substrate (Merck Millipore) was added prior to imaging using the ChemiDoc Imaging System with ImageLab software (Bio-Rad).
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bioRxiv - Cancer Biology 2024Quote: ... Luminata Forte Western horseradish peroxidase (HRP) substrate (Merck Millipore) was used for developing the signal and membranes were imaged and analyzed using the ChemiDoc Imaging System with ImageLab software (Bio-Rad) ...
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bioRxiv - Plant Biology 2021Quote: ... and horseradish peroxidase (Merck, P8125-5KU, 0.125 units per reaction). Elicitors flg22 (Genscript ...
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bioRxiv - Cell Biology 2021Quote: ... Conjugated goat-anti mouse immunoglobulin G (IgG)-horseradish peroxidase (HRP) (Merck, A4416) or anti-rabbit IgG (whole molecule)–peroxidase antibody produced in goat (MERCK ...
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bioRxiv - Cancer Biology 2019Quote: ... The secondary horseradish peroxidase-coupled goat anti-mouse-IgG (AP124P, Merck Millipore, Darmstadt, Germany) for β-actin antibody was 10,000-fold diluted and goat anti-rabbit-IgG (81-6120 ...
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bioRxiv - Molecular Biology 2019Quote: ... Immunosignals were detected using the horseradish peroxidase substrates Luminata Classico or Crescendo (Merck Millipore) or Femto (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... with an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP; Merck, Darmstadt, Germany).
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bioRxiv - Immunology 2024Quote: ... 30 µL/well of the Luminol mix containing 80 U/mL Horseradish peroxidase (Merck) and 8 mM Luminol (AppliChem Life Science ...
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bioRxiv - Developmental Biology 2021Quote: ... plus 5 % sheep serum in MAB and incubated with anti-DIG conjugated to Peroxidase (1/50; Merck) in 1 % blocking reagent diluted in MAB for 3 days at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... The water was then replaced with a solution containing 10 μg/ml horseradish peroxidase (HRP) (Merck), 100 μM of the luminol derivate L-012 (Wako Chemicals ...
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bioRxiv - Immunology 2023Quote: ... Bound antibodies were detected with the horseradish peroxidase (HRP) substrate o-phenylenediamine (Merck KGaA, Darmstadt, Germany). The reactions were stopped with 1 M H2SO4 after 20 min and the absorbance was measured in a microplate reader (Benchmark ...
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bioRxiv - Genomics 2023Quote: ... incubated in the secondary antibody horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Merck 12-348) (1 h ...
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bioRxiv - Neuroscience 2023Quote: ... MOG (myelin oligodendrocyte glycoprotein, Cat#MAB5622, Merck Millipore) and NFH (neurofilament heavy chain ...
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bioRxiv - Molecular Biology 2021Quote: ... and a horseradish peroxidase (HRP)-conjugated donkey anti-goat IgG as secondary antibody (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Cell Biology 2023Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:8,000, Merck Millipore) and visualised with the LiteAblot Plus substrate (Euroclone) ...
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bioRxiv - Immunology 2023Quote: ... or 1% FCS (Biowest) and 2 mM CaCl2 and 2 mM MgCl2 (Merck).
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bioRxiv - Microbiology 2023Quote: ... S1 (N5661-50KU, Merck), and Micrococcal nuclease (EN0181 ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 1% FCS (Biowest) and 2 mM CaCl2 and 2 mM MgCl2 (Merck) prior to adding the cells.
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bioRxiv - Cell Biology 2021Quote: ... Immuno-reactive complexes were detected using horseradish-peroxidase coupled anti-rabbit or anti-mouse antibodies (Sigma-Aldrich/Merck, Germany) and subsequent detection of chemiluminescence signals with a ChemoCam Camera system (INTAS Science instruments GmbH ...
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bioRxiv - Microbiology 2020Quote: ... anti-A17L (1:500) in combination with horseradish peroxidase (HRP)-coupled secondary antibodies (1:2,000) and Immoblion Forte Western HRP substrate (Merck) or with IR-Dye secondary antibodies (Licor ...
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bioRxiv - Cell Biology 2024Quote: ... Blots were washed three times in TBS-T followed by incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck). Following washing in TBS-T ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were detected with a horseradish peroxidase (HRP)-conjugated secondary antibody (1:3000, #170-6515, Biorad or #12-349, Merck Millipore ...
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bioRxiv - Cancer Biology 2019Quote: ... Secondary antibody incubation was performed at room temperature for 90 min utilizing a horseradish-peroxidase (HRP)-labeled anti-rabbit IgG (Merck), and staining was visualized with DAB (Dako ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by washing and staining with horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG secondary antibodies (1:5,000 dilution; Merck Millipore), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... Plates were washed three times with 0.1% PBST and incubated at RT for 1 hour with horseradish peroxidase-conjugated secondary antibody (Cat No. NA934, Merck Millipore) diluted 1:1,000 in 0.05% PBST ...
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bioRxiv - Neuroscience 2023Quote: ... washed in TBS-T and incubated with horseradish peroxidase–conjugated anti-rabbit (1:2000, #7074P2, Cell Signalling or 1:80000, #A0545, Merck) and anti-mouse (1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... fluorescent visualization of the nonisotopic signals used anti-digoxigenin antibodies conjugated to horseradish peroxidase (anti-digoxigenin-POD; Fab fragment; 1/200; Merck). This was followed by the deposition of biotin tyramide (1:1000 ...
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bioRxiv - Biochemistry 2024Quote: ... H2O was removed and ROS production was measured upon addition of a 100-μL assay solution which contained 10 μg/mL horseradish peroxidase (P6782, Merck), 10 mM luminol and the treatment (1 μM SCOOP ...
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bioRxiv - Biochemistry 2023Quote: ... the Siglec-7-Spy Tag-TEV-Fc was concentrated using an AMICON (Molecular Weight Cut Off 30 kDa – Merck Millipore) to approximately 0.15 mg/ml measured via NanoDrop as described above ...
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bioRxiv - Genomics 2022Quote: ... Keratanase (G6920-5UN)) and a glycoprotein deglycosylation kit (Merck catalog #362280). We then used Water-18O (97% atom ...
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bioRxiv - Developmental Biology 2020Quote: ... membranes were washed 3 times with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP (Horseradish Peroxidase) Substrate (Merck Millipore) using a Gel-Doc XR+ System (BioRad) ...
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bioRxiv - Microbiology 2021Quote: 2mL SARS-CoV-2 P4 supernatant containing 2 x 107 pfu/mL was purified using an Amicon Ultra column (MWCO 100kDa; Merck) by centrifugation and washing in PBS at 2,000 x g ...
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bioRxiv - Microbiology 2019Quote: ... sheep α-rabbit HRP (AP510P, Merck) and goat α-mouse HRP (STAR117P ...
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bioRxiv - Developmental Biology 2023Quote: ... and 5% normal sheep serum (Merck). Incubation with primary antibodies was done for 12 to 72 hours at 8 °C in PBS-T with 3% BSA and 0.02% sodium azide ...
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bioRxiv - Microbiology 2021Quote: ... The binding of CoVHH1-FLAG to S1 subunits-His tag was detected by incubating for 1 hour at room temperature with mouse monoclonal ANTI-FLAG® M2-Peroxidase (Merck) diluted with PBST ...
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bioRxiv - Immunology 2022Quote: ... Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining was performed using an Apop Tag Plus Peroxidase In Situ Apoptosis Kit (Merck Millipore) following the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were washed and probed with secondary antibody for one hour at RT (anti-mouse IgG, horseradish peroxidase linked; 1:5,000; Merck, Burlington, MA, USA). Bands were visualized using high sensitivity Pierce ECL Plus (Thermo Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Immunoreactive bands were detected using iBright CL1000 chemiluminator or LAS 3000 (Fujifilm, Tokyo, Japan) with an enhanced chemiluminescent substrate for the detection of horseradish peroxidase (HRP; Merck, Darmstadt, Germany). The intensity of the RAS band was quantified using ImageJ software and normalized to the intensity of the GAPDH band.
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bioRxiv - Cancer Biology 2023Quote: ... Immunoreactive bands were detected with horseradish peroxidase-coupled anti-mouse or anti-rabbit antibodies (CST) using the ECL System (Merck Millipore; WBKLS0500). Immunoblot analysis was performed using the LI-COR Odyssey Imaging System.
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bioRxiv - Molecular Biology 2023Quote: ... The detection was performed using a specific secondary antibody coupled to the horseradish peroxidase (HRP): anti-mouse-HRP (1:4000, A4416 Merck, Sigma Aldrich), anti-rabbit-HRP (1:10000 ...
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bioRxiv - Neuroscience 2020Quote: ... sheep anti-Neuropeptide Y (1:1000; Merck), rabbit anti-KCNQ2 and anti-HCN2 (1:200 ...