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Citations for Merck :
1 - 50 of 2302 citations for SARS CoV 2 Spike E M Mosaic Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Microbiology 2020Quote: ... 70-230 mesh silica 60 (E. M. Merck) was used as the stationary phase ...
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bioRxiv - Biophysics 2020Quote: ... After removal of the poly-E tag with the 3C protease (MERCK), the protein was again subjected to gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: 10His-tagged MA gene was expressed in Escherichia coli (E. coli) strain BL21 (DE3) (Merck, Darmstadt, Germany). The 10His-tagged MA protein was initially purified with nickel-NTA affinity column chromatography followed by TEV cleavage ...
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bioRxiv - Microbiology 2022Quote: ... The sections were then incubated overnight in PBS with 0.2% BSA and 0.05% Tween-20 with primary antibodies directed against SARS-CoV-2 Nucleocapsid Protein (1:500; mouse monoclonal, # ZMS1075, Merck); Iba1 (1 ...
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bioRxiv - Molecular Biology 2023Quote: ... and 0.1 mM 2-ß-mercaptoethanol (Merck ES-007-E), on 0.2% gelatin-coated plates ...
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bioRxiv - Biophysics 2024Quote: ... 0.5 x EmbryoMax 2-Mercaptoethanol (Merck Millipore #ES-007-E), 2.5 μg/ml Plasmocin (Invivogen) ...
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bioRxiv - Biochemistry 2023Quote: ... Silica gel (E, Merck) was used for flash chromatography ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred Immobilon-E PVDF membrane (Merck Millipore, Tullagreen, Ireland) and AGAL was detected by monoclonal anti-Galactosidase alpha antibody (# ab168341 ...
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bioRxiv - Biophysics 2020Quote: ... After removal of the poly-E tag located on the “alive” subunit with 3C protease (MERCK) followed by a gel filtration step (Superdex-200 10/300 GE Healthcare Life Sciences) ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... high ethanol concentration (E. Merck), Xylol ...
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bioRxiv - Biochemistry 2020Quote: ... HRP-conjugated streptavidin (E. Merck), 3.3 ‘diaminobenzidine (DAB) ...
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bioRxiv - Cell Biology 2023Quote: ... anti-E-cadherin (#MABT26, Merck), anti-Paxillin (#GTX125891 ...
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bioRxiv - Microbiology 2022Quote: ... After testing for SARS-CoV-2 by PCR test (COBAS 6800, Merck México, Mexico city), they were classified as positive (AP ...
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... E-cadherin (1:300, Merck, #U3254), α-actinin (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... E-Cadherin (1:600, Merck U3254), Glucagon (1:500 ...
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bioRxiv - Systems Biology 2023Quote: ... Primary his tag antibody (05-949, Merck), stored in a skim milk solution ...
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bioRxiv - Genetics 2021Quote: ... Proteins separated by SDS-PAGE were transferred onto a PVDF membrane (Immobilon-E, Merck) and blocked in 5% milk in Tris-Buffer Saline (TBS ...
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bioRxiv - Microbiology 2022Quote: ... The SARS CoV-2 glycoproteins were then purified by divalent cation affinity chromatography using TALON resin (Merck) or HiTrap IMAC FF followed by size exclusion chromatography using a Superose 6 Increase 10/300 column linked to an AKTApure instrument (Cytiva) ...
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bioRxiv - Neuroscience 2021Quote: ... and coverslipped with Entellan (E. Merck, Switzerland).
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bioRxiv - Microbiology 2020Quote: ... treatment with 80 µM E-64d (Merck), 80 µM Z-VAD-fmk (Invivogen ...
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bioRxiv - Molecular Biology 2020Quote: ... WT—or its mutants fused with a His-tag on the amino-terminus—were expressed in Escherichia coli BL21 (DE3) using pET15b vector (Merck). A single colony was inoculated in 2 mL Luria-Bertani broth (LB ...
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bioRxiv - Microbiology 2022Quote: ... the Milliplex SARS-CoV-2 Antigen Panel 1 IgG was used according to the manufacturer’s instructions (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Cell Biology 2021Quote: ... The separated proteins were then transferred to the PVDF membranes (0.45 µm Immobilon®-E; Merck Millipore). Membranes were blocked either with 2.5% nonfat milk in TBST (50 mM Tris ...
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bioRxiv - Biochemistry 2019Quote: EcSerRS (from E. coli strain B ER2560) and SaSerRS (from S. aureus seg50 (1150)) were cloned into pET52b(+) vector (Merck Millipore, Germany) using the NcoI and SacI restriction sites allowing for the production of protein with a thrombin cleavable C-terminal His10-tag ...
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bioRxiv - Microbiology 2020Quote: ... To investigate the inhibitory activity of E-64 (Merck) and Z-VAD-fmk (Invivogen ...
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bioRxiv - Microbiology 2019Quote: ... we provided the information of praziquantel (E. Merck KG) written in Chichewa to all villagers ...
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bioRxiv - Cell Biology 2023Quote: ... Ultrathin sections were stained with uranyl acetate (E. Merck) and lead citrate (Sigma-Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Human HepaRG hepatoma cells (1.5-2×105 cells/cm2, Biopredic International, Rennes, France) were cultured in William’s E medium (Merck) supplemented with 10% heat-inactivated FCS ...
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bioRxiv - Biochemistry 2020Quote: ... Materials for staining tissue include: Phosphate buffered formalin (E. Merck), high ethanol concentration (E ...
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bioRxiv - Cancer Biology 2022Quote: ... and stained with haematoxylin and eosin (H&E; Merck + Sigma) or IHC using antibodies of interest ...
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bioRxiv - Molecular Biology 2020Quote: ... transferred to the Immobilon-P/E PVDF membrane (Merck Millipore), and immunodetected using the SuperSignal West Pico PLUS Chemiluminescent Substrate (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 1-2.5 × 105 CFU-E/proerythroblasts isolated from Dox-treated mice were seeded on fibronectin-coated (2 µg/mL; Merck) 48-well plates in Iscove’s modified Dulbecco’s medium (IMDM ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μL of each sample was injected into a Chromolith FastGradient RP 18-e column (50 x 3 x 2 mm; monolithic silica with bimodal pore structure, macropores with 1.6mm diameter; Merck) protected by a precolumn (Gemini NX RP18 ...
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bioRxiv - Microbiology 2022Quote: ... 5°6.7’ E) in September and October 2017 by filtering 2 L of seawater through PVDF membrane filters (Merck Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... and applied directly onto a column of 2 ml pre-equilibrated Ni-NTA beads (cOmplete His-Tag Purification Resin from Merck). After several washes with high salt (500 mM NaCl) ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was loaded on to a gravity flow-based Ni-NTA metal affinity column (2 ml beads, cOmplete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a gravity flow-based Ni-NTA metal affinity column (2 ml beads, complete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... H&E staining was carried out using a kit (Merck, Germany). Muscle fiber cross sectional area was measured with the ImageJ 1.48 software (National Institutes of Health ...
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bioRxiv - Physiology 2020Quote: ... 3.) C + 0.3 g/kg vitamin E (Merck KGaA, Darmstadt, Germany) (vit E diet) ...
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bioRxiv - Physiology 2024Quote: ... sections were stained with hematoxylin and eosin (H&E; Merck, Zug, Switzerland).
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bioRxiv - Molecular Biology 2021Quote: ... coli Rosetta 2 cells (Merck) by induction with 0.2 mM isopropyl β-d-1-thiogalactopyranoside for 18 h at 18 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... coli Rosetta 2 (DE3) (Merck) and purified using Glutathione Sepharose High Performance beads (GE Healthacare ...
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bioRxiv - Microbiology 2023Quote: ... 2 M MgSO4 (Merck), 670 mM CaCl2 (Merck ...
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bioRxiv - Microbiology 2023Quote: ... 2 M NaCl (Merck), 2 M KCl (Merck) ...
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bioRxiv - Microbiology 2023Quote: ... 2 M KCl (Merck), 2 M MgSO4 (Merck) ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...