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Citations for Merck :
1 - 50 of 1735 citations for Recombinant Mouse Pcsk9 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... His tagged proteins were labeled using Ni-NTA-ATTO 488 (Merck Group) at a ratio of ATTO/protein = 1/500 in MN buffer (50 mM MES pH 6 ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... The eluted His-Myc tagged recombinants were further concentrated using 3kDa ultra concentration units (Merck, Germany). The GST-tagged recombinant proteins were affinity purified using Glutathione Sepharose 4B beads and purified in batch mode ...
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bioRxiv - Plant Biology 2020Quote: ... His-tagged proteins were washed and purified using the Amicon Pro System (Merck) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: His-tagged proteins were labeled using Ni-NTA-ATTO 550 or Ni-NTA-ATTO 647 from Merck Group ...
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bioRxiv - Genomics 2019Quote: ... and 100 Units/ml of Recombinant mouse LIF Protein (Merck ESG1107). Culture were seeded at an average density of ∼ 3.8*104 cells/cm2 and passaged every 48 h.
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bioRxiv - Molecular Biology 2024Quote: ... FLAG-tagged proteins were detected using M2 anti-FLAG antibody (mouse, F1084, Merck) followed and fluorescent Anti-Mouse Alexa Fluor™ Plus 800 ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt (50mM Tris pH8.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt buffer (50mM Tris pH7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The His6- tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with 2mL Ni-NTA His•Bind® Resin (Merck), followed by incubation with TEV protease for 12 hours at 4 °C to remove the His6 tag ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant active DAPK3 protein was obtained from Merck.
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bioRxiv - Cell Biology 2021Quote: ... Myc-tagged proteins were immuno-precipitated with the 9E10 antibody (Merck); Rpb1 was immunoprecipitated using the 8WG16 antibody (Merck).
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitation of cell lysates overexpressing FLAG-tagged or GST-tagged proteins were performed using anti-FLAG-M2 affinity beads (Merck) or glutathione-Sepharose beads (Cytiva).
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bioRxiv - Neuroscience 2023Quote: Biotinylated surface proteins were isolated using streptavidin-coated agarose beads (Merck). The beads were washed 2 times in lysis buffer by centrifugation at low speed (<1.5k RCF) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged Trx1 was purified on a Ni2+ sepharose resin following the manufactureŕs instructions (cOmplete™ His-Tag Purification Resin, Merck).
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bioRxiv - Microbiology 2020Quote: ... GST bound protein was then cleaved using biotinylated thrombin (Merck Millipore; 69672) according to the manufacturers instructions overnight at 4°C ...
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... of the following ECM proteins: recombinant human fibronectin (Merck, 341631), recombinant human vitronectin (PeproTech ...
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bioRxiv - Microbiology 2022Quote: ... and the FLAG-tagged proteins were immunoprecipitated with anti-FLAG agarose (A2220, MERCK). Input samples were collected as described above.
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 2020) containing 300 ng GST-tagged MP-GAP fragments and 200 ng recombinant human Aurora A kinase (Merck, 14-511), 1mCi of [gamma-P32]ATP (Hartmann Analytic ...
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bioRxiv - Genomics 2020Quote: ... ESGRO recombinant mouse Leukaemia Inhibitory Factor (LIF) was obtained from Merck Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... and a biotinylated donkey antibody to mouse IgG (10 μg/ml; AP192B, Merck Millipore). The sections were further incubated with Alexa594-conjugated streptavidin (2.5 μg/ml ...
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bioRxiv - Biophysics 2020Quote: ... 100 μl of 10 μg/ml recombinant protein G (Merck, Darmstadt, Germany) was added and incubated for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were treated with recombinant mouse TNFα (10 ng/ml, Merck 654245).
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bioRxiv - Biochemistry 2020Quote: ... Flag-tagged proteins were eluted by incubation with 150 μg/mL Flag tag peptide (Merck; F3290) with 0.1% digitonin in solubilisation buffer for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg purified recombinant LARK protein or 1 μg BG4 (MABE917, Merck, Darmstadt, Germany) was added ...
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bioRxiv - Molecular Biology 2021Quote: ... StrepII-tagged proteins (RBBP6, symplekin and WDR33) were detected using streptavidin-HRP conjugate (Merck Millipore, cat. No. 18152) and Amershan ECL Detection Reagents (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... The expression of S-tagged NrdA was confirmed by purification using S-protein agarose (Merck Millipore, Darmstadt, Germany) as previously described (11 ...
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bioRxiv - Cell Biology 2020Quote: ... Purification of His6-tagged proteins from the soluble fraction was achieved using Ni-NTA His.Bind® Resin (Merck), a peristaltic pump and a series of column washes with buffers of increasing imidazole content ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... or anti-His (Merck) for His-G5845–1508) ...
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bioRxiv - Cancer Biology 2019Quote: The uncharged polystyrene 96-well plate were coated with different recombinant proteins-Collagen IV (Merck Millipore), P-Selectin ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were obtained after expression at 22°C in B834 (DE3) cells (Merck, Darmstadt, Germany), upon induction with 0.6□mM IPTG at 0.8 OD600 ...
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bioRxiv - Cell Biology 2021Quote: ... Virotrap particles containing protein complexes were purified in a single step using biotinylated anti-FLAG BioM2 antibody (#F9291, Merck) and Dynabeads MyOne Streptavidin T1 Beads (#65601 ...
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bioRxiv - Biochemistry 2023Quote: ... and transiently transfected with the plasmid DNA containing GFP-tagged protein of interest (See Table S7 for plasmid constructs) using GeneJuice transfection reagent (70967, Merck) according to manufacturer’s protocol ...
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bioRxiv - Biophysics 2019Quote: ... fractions containing monomeric PSCFP2*-AVI-3C-12xHis were concentrated using Amicon Ultra-4 centrifugal filters (10 kDa cut-off, Merck) in the presence of 100 µM Tris(2-carboxyethyl)phosphine (TCEP ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 10 ng/ml of the recombinant human TNFα protein (Merck-Millipore, Cat# GF023) and the outcome was assessed at RNA or protein levels.
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing the desired His6-tagged protein were concentrated to 2 ml using Amicon® Ultra Centrifugal Filters (10,000 MWCO, Merck Millipore), and were directly injected into a size-exclusion chromatography column (Superdex 75 16/60 ...
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bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat. No 71403). Protein expression was always performed using freshly transformed chemically competent E ...
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bioRxiv - Molecular Biology 2019Quote: ... Biotinylated porcine intestinal mucosa heparin (Merck Millipore) was immobilized on streptavidin biosensors at 10 µg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-neurofilament-H protein (clone NE14; Merck, Darmstadt, Germany); mouse anti-dephosphorylated neurofilament protein (clone smi32 ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-neurofilament-H protein (clone NE14; Merck, Darmstadt, Germany); rabbit anti-IBA1 (Polyclonal IgG ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the mouse anti-ubiquitinated proteins 1:1000 (04-263, Merck), the rabbit anti-LC3B 1:1000 (NB600-1384 ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...