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Citations for Merck :
1 - 50 of 1233 citations for Recombinant E. coli NAGK Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat. No 71403). Protein expression was always performed using freshly transformed chemically competent E ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Microbiology 2021Quote: 10His-tagged MA gene was expressed in Escherichia coli (E. coli) strain BL21 (DE3) (Merck, Darmstadt, Germany). The 10His-tagged MA protein was initially purified with nickel-NTA affinity column chromatography followed by TEV cleavage ...
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant active DAPK3 protein was obtained from Merck.
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bioRxiv - Cell Biology 2023Quote: ... of the following ECM proteins: recombinant human fibronectin (Merck, 341631), recombinant human vitronectin (PeproTech ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred Immobilon-E PVDF membrane (Merck Millipore, Tullagreen, Ireland) and AGAL was detected by monoclonal anti-Galactosidase alpha antibody (# ab168341 ...
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bioRxiv - Genomics 2019Quote: ... and 100 Units/ml of Recombinant mouse LIF Protein (Merck ESG1107). Culture were seeded at an average density of ∼ 3.8*104 cells/cm2 and passaged every 48 h.
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bioRxiv - Biochemistry 2022Quote: ... Selenomethionine-labelled proteins were expressed in Escherichia coli B834(DE3) cells (Merck). All other proteins were expressed in Escherichia coli BL21(DE3 ...
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bioRxiv - Biophysics 2020Quote: ... 100 μl of 10 μg/ml recombinant protein G (Merck, Darmstadt, Germany) was added and incubated for 30 minutes ...
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bioRxiv - Genetics 2021Quote: ... Proteins separated by SDS-PAGE were transferred onto a PVDF membrane (Immobilon-E, Merck) and blocked in 5% milk in Tris-Buffer Saline (TBS ...
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bioRxiv - Biochemistry 2019Quote: EcSerRS (from E. coli strain B ER2560) and SaSerRS (from S. aureus seg50 (1150)) were cloned into pET52b(+) vector (Merck Millipore, Germany) using the NcoI and SacI restriction sites allowing for the production of protein with a thrombin cleavable C-terminal His10-tag ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg purified recombinant LARK protein or 1 μg BG4 (MABE917, Merck, Darmstadt, Germany) was added ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... coli (Merck). Cells were grown at 37 °C in lysogen broth medium supplemented with 50 μg .mL−1 kanamycin (and 34 μg.mL−1 of chloramphenicol for Rosetta ...
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bioRxiv - Cell Biology 2022Quote: All proteins used in this study were overexpressed in Rosetta (DE3, pLysS) Escherichia coli (MERCK) cells using 0.4 mM isopropyl 1-thio-D-galactopyranoside (Inalco Pharmaceuticals ...
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bioRxiv - Cell Biology 2021Quote: ... The separated proteins were then transferred to the PVDF membranes (0.45 µm Immobilon®-E; Merck Millipore). Membranes were blocked either with 2.5% nonfat milk in TBST (50 mM Tris ...
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bioRxiv - Microbiology 2022Quote: Wild type and mutant CANC proteins were expressed in Escherichia coli Rosetta (DE3) cells (Merck Millipore) as previously described (Betancor et al. ...
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bioRxiv - Cancer Biology 2019Quote: The uncharged polystyrene 96-well plate were coated with different recombinant proteins-Collagen IV (Merck Millipore), P-Selectin ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were obtained after expression at 22°C in B834 (DE3) cells (Merck, Darmstadt, Germany), upon induction with 0.6□mM IPTG at 0.8 OD600 ...
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bioRxiv - Biochemistry 2023Quote: ... Silica gel (E, Merck) was used for flash chromatography ...
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Actin binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin IIbioRxiv - Biophysics 2022Quote: ... coli (Merck Millipore), and purified basically in the same way as Rng2CHD ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 10 ng/ml of the recombinant human TNFα protein (Merck-Millipore, Cat# GF023) and the outcome was assessed at RNA or protein levels.
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bioRxiv - Biochemistry 2020Quote: ... high ethanol concentration (E. Merck), Xylol ...
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bioRxiv - Biochemistry 2020Quote: ... HRP-conjugated streptavidin (E. Merck), 3.3 ‘diaminobenzidine (DAB) ...
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bioRxiv - Cell Biology 2023Quote: ... anti-E-cadherin (#MABT26, Merck), anti-Paxillin (#GTX125891 ...
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bioRxiv - Microbiology 2020Quote: ... coli ROSETTA strain (Merck) using pE-SUMO as an expression plasmid (86 ...
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bioRxiv - Cell Biology 2021Quote: ... coli BL21 (Novagen/ Merck Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... coli Rosetta2 (DE3) (Merck); TALON Metal Affinity Resin (Clontech) ...
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bioRxiv - Biochemistry 2023Quote: ... coli Rosetta2 (DE3) (Merck) was transformed using the constructed plasmid and cultured at 37°C in LB medium containing 30 μg/ml kanamycin and 34 μg/ml chloramphenicol ...
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bioRxiv - Biochemistry 2024Quote: ... coli C41(DE3) (Merck) co-transformed with pET22-Wscytc and pEC86 (which harbors the ccm genes for production of the machinery for c-type heme biosynthesis and maturation (Arslan et al. ...
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... E-cadherin (1:300, Merck, #U3254), α-actinin (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... E-Cadherin (1:600, Merck U3254), Glucagon (1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli Rosetta 2 cells (Merck) by induction with 0.2 mM isopropyl β-d-1-thiogalactopyranoside for 18 h at 18 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... coli Rosetta 2 (DE3) (Merck) and purified using Glutathione Sepharose High Performance beads (GE Healthacare ...
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bioRxiv - Cell Biology 2019Quote: ... coli Rosetta2 pLysS (Merck 71403). Overnight cultures were diluted 1:50 in a final volume of 50 ml ...
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bioRxiv - Cell Biology 2019Quote: ... coli Rosetta2 pLysS (Merck 71403) were used ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21 (DE3) cells (Merck) and the mutant proteins were expressed and purified according to the protocol described by Oliver ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Coli BL21 (DE3) cells (Merck) were transformed with the pET28a(+)-scDH and pET28a(+)-ΔscDH and grown in LB medium with 35 μg/ml kanamycin at 37 °C and shaken until an OD600 of ∼ 0.6 ...
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bioRxiv - Molecular Biology 2022Quote: ... coli BL21(DE3) star (Merck). Luria Bertani (LB ...
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bioRxiv - Genetics 2022Quote: ... coli O111:B4 (#LPS25, Merck) or Pam2CSK4 (#tlrl-pm2s-1 ...
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bioRxiv - Immunology 2023Quote: ... coli serotype O55:B5 (Merck) for 24 to 48 hours to arrive at a mature BMDC culture or alternatively frozen to -80°C in 90% FCS (Biowest ...
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bioRxiv - Immunology 2023Quote: ... coli serotype O55:B5 (Merck) for 24 to 48 hours to induce B-cell blast formation.
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bioRxiv - Microbiology 2023Quote: ... coli expression vector (Merck-Novagen) using Gibson assembly ...
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bioRxiv - Cancer Biology 2020Quote: ... The glutathione-S-transferases (GST) fusion proteins were expressed in Escherichia coli and purified with glutathione-conjugated agarose beads (Merck) by standard procedures ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant BG4 (recBG4) (Millipore Merck) was added as control.
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bioRxiv - Biochemistry 2020Quote: ... The recombinant protein was dialyzed overnight using an Amicon Ultra 15 mL spin dialysis column 10 000 MWCO (Merck, Germany). Purified protein was quantified using Bradford’s assay ...
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bioRxiv - Neuroscience 2023Quote: ... the affinity resin was prepared by incubating 100 µg of recombinant protein with 20 µl of nickel beads (PureProteome Nickel magnetic beads, Merck) in 50 mM Pull-Down Buffer PDB (Tris–HCl pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... Affinity-purified recombinant TrK13 proteins (WT, C580Y, R539T and C580Y) or commercially purchased BSA (A7030) or lysozyme (L6876) (Merck, Germany), each at 10 µM concentration ...