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Citations for Merck :
1 - 50 of 325 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Microbiology 2021Quote: 10His-tagged MA gene was expressed in Escherichia coli (E. coli) strain BL21 (DE3) (Merck, Darmstadt, Germany). The 10His-tagged MA protein was initially purified with nickel-NTA affinity column chromatography followed by TEV cleavage ...
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bioRxiv - Immunology 2023Quote: ... The eluted His-Myc tagged recombinants were further concentrated using 3kDa ultra concentration units (Merck, Germany). The GST-tagged recombinant proteins were affinity purified using Glutathione Sepharose 4B beads and purified in batch mode ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... His tagged proteins were labeled using Ni-NTA-ATTO 488 (Merck Group) at a ratio of ATTO/protein = 1/500 in MN buffer (50 mM MES pH 6 ...
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bioRxiv - Plant Biology 2020Quote: ... His-tagged proteins were washed and purified using the Amicon Pro System (Merck) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: His-tagged proteins were labeled using Ni-NTA-ATTO 550 or Ni-NTA-ATTO 647 from Merck Group ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt (50mM Tris pH8.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt buffer (50mM Tris pH7.0 ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged Trx1 was purified on a Ni2+ sepharose resin following the manufactureŕs instructions (cOmplete™ His-Tag Purification Resin, Merck).
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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bioRxiv - Biochemistry 2022Quote: ... The His6- tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with 2mL Ni-NTA His•Bind® Resin (Merck), followed by incubation with TEV protease for 12 hours at 4 °C to remove the His6 tag ...
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bioRxiv - Cell Biology 2023Quote: ... 2020) containing 300 ng GST-tagged MP-GAP fragments and 200 ng recombinant human Aurora A kinase (Merck, 14-511), 1mCi of [gamma-P32]ATP (Hartmann Analytic ...
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bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat. No 71403). Protein expression was always performed using freshly transformed chemically competent E ...
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bioRxiv - Molecular Biology 2020Quote: ... WT—or its mutants fused with a His-tag on the amino-terminus—were expressed in Escherichia coli BL21 (DE3) using pET15b vector (Merck). A single colony was inoculated in 2 mL Luria-Bertani broth (LB ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Biochemistry 2019Quote: EcSerRS (from E. coli strain B ER2560) and SaSerRS (from S. aureus seg50 (1150)) were cloned into pET52b(+) vector (Merck Millipore, Germany) using the NcoI and SacI restriction sites allowing for the production of protein with a thrombin cleavable C-terminal His10-tag ...
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Cell Biology 2024Quote: ... coli (Merck). Cells were grown at 37 °C in lysogen broth medium supplemented with 50 μg .mL−1 kanamycin (and 34 μg.mL−1 of chloramphenicol for Rosetta ...
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bioRxiv - Molecular Biology 2022Quote: ... or anti-His (Merck) for His-G5845–1508) ...
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bioRxiv - Clinical Trials 2019Quote: ... competitive angiotensin II type 1 receptor antagonist losartan (LT, Cozaar; Merck, USA) or placebo (PLC) ...
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bioRxiv - Biochemistry 2023Quote: ... Silica gel (E, Merck) was used for flash chromatography ...
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Actin binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin IIbioRxiv - Biophysics 2022Quote: ... coli (Merck Millipore), and purified basically in the same way as Rng2CHD ...
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bioRxiv - Biochemistry 2020Quote: ... high ethanol concentration (E. Merck), Xylol ...
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bioRxiv - Biochemistry 2020Quote: ... HRP-conjugated streptavidin (E. Merck), 3.3 ‘diaminobenzidine (DAB) ...
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bioRxiv - Cell Biology 2023Quote: ... anti-E-cadherin (#MABT26, Merck), anti-Paxillin (#GTX125891 ...
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bioRxiv - Microbiology 2020Quote: ... coli ROSETTA strain (Merck) using pE-SUMO as an expression plasmid (86 ...
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bioRxiv - Cell Biology 2021Quote: ... coli BL21 (Novagen/ Merck Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... coli Rosetta2 (DE3) (Merck); TALON Metal Affinity Resin (Clontech) ...
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bioRxiv - Biochemistry 2023Quote: ... coli Rosetta2 (DE3) (Merck) was transformed using the constructed plasmid and cultured at 37°C in LB medium containing 30 μg/ml kanamycin and 34 μg/ml chloramphenicol ...
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bioRxiv - Biochemistry 2024Quote: ... coli C41(DE3) (Merck) co-transformed with pET22-Wscytc and pEC86 (which harbors the ccm genes for production of the machinery for c-type heme biosynthesis and maturation (Arslan et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitation of cell lysates overexpressing FLAG-tagged or GST-tagged proteins were performed using anti-FLAG-M2 affinity beads (Merck) or glutathione-Sepharose beads (Cytiva).
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bioRxiv - Immunology 2020Quote: ... 350 ng/mL active ERK2-GST tagged (Merck) or 175 ng/mL ERK1 plus 175 ng/mL ERK2-GST for 30 min at 30°C.
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... E-cadherin (1:300, Merck, #U3254), α-actinin (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... E-Cadherin (1:600, Merck U3254), Glucagon (1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli Rosetta 2 cells (Merck) by induction with 0.2 mM isopropyl β-d-1-thiogalactopyranoside for 18 h at 18 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... coli Rosetta 2 (DE3) (Merck) and purified using Glutathione Sepharose High Performance beads (GE Healthacare ...
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bioRxiv - Cell Biology 2019Quote: ... coli Rosetta2 pLysS (Merck 71403). Overnight cultures were diluted 1:50 in a final volume of 50 ml ...
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bioRxiv - Cell Biology 2019Quote: ... coli Rosetta2 pLysS (Merck 71403) were used ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21 (DE3) cells (Merck) and the mutant proteins were expressed and purified according to the protocol described by Oliver ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Coli BL21 (DE3) cells (Merck) were transformed with the pET28a(+)-scDH and pET28a(+)-ΔscDH and grown in LB medium with 35 μg/ml kanamycin at 37 °C and shaken until an OD600 of ∼ 0.6 ...
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bioRxiv - Molecular Biology 2022Quote: ... coli BL21(DE3) star (Merck). Luria Bertani (LB ...
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bioRxiv - Genetics 2022Quote: ... coli O111:B4 (#LPS25, Merck) or Pam2CSK4 (#tlrl-pm2s-1 ...
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bioRxiv - Immunology 2023Quote: ... coli serotype O55:B5 (Merck) for 24 to 48 hours to arrive at a mature BMDC culture or alternatively frozen to -80°C in 90% FCS (Biowest ...
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bioRxiv - Immunology 2023Quote: ... coli serotype O55:B5 (Merck) for 24 to 48 hours to induce B-cell blast formation.
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bioRxiv - Microbiology 2023Quote: ... coli expression vector (Merck-Novagen) using Gibson assembly ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% HI-FBS (Merck/Biochrom), 2 mM L-glutamine (Merck/Biochrom) ...
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bioRxiv - Systems Biology 2023Quote: ... Primary his tag antibody (05-949, Merck), stored in a skim milk solution ...