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Citations for Merck :
1 - 50 of 164 citations for Real Time Thermal Cycler Thermocycler Accessories since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Real-time quantitative PCR was performed using 5uL of KAPA Sybr Fast Universal 2x PCR master mix (Merck, #KK4601), 0.6uL of 5 uM forward primer ...
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bioRxiv - Molecular Biology 2020Quote: ... Real time quantitative PCR was performed with KAPA SYBR FAST qPCR Master Mix (Sigma-Aldrich - Merck, UK, cat no KK4602) according to manufacturer’s instructions in a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 5 μL of each sample were mixed with 7.5 μL of KAPA probe fast universal real-time PCR master mix (Merck, Darmstadt, Germany) and 2.5 μL of the indicated primer/probe combinations ...
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bioRxiv - Synthetic Biology 2021Quote: ... GGA reactions were performed in a thermocycler according to a standard GGA protocol.52 The GGA products were transformed into NovaBlue cells (Merck), from which the plasmids were purified using the QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Genomics 2021Quote: ... with A pestle ~10 times following B pestle ~20 times in lysis buffer [5 mM CaCl2 (Merck, A546282), 3 mM Mg(Ac)2 (Roth ...
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bioRxiv - Molecular Biology 2019Quote: ... was washed 3 times with PBS (Merck Millipore ...
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bioRxiv - Biophysics 2021Quote: ... A biotinylated oligonucleotide was hybridized at 5x molar excess to a protruding elongated staple strand during the initial thermal annealing process of the DNA origami tile followed by purification using 100kDa Amicon®Ultra centrifugal filters (Merck). In a next step ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Single product was validated on 1.5% agarose gel after incubated at 95 °C for two minutes in thermo cycler with hot lid then brought to 22 °C and mixed with formamide (Merck millipore) in 1:3 ratio ...
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bioRxiv - Microbiology 2021Quote: ... Coverslips were washed ten times in PBS and ten times in ultrapure water before mounting on slides using Mowiol 4–88 (Merck) containing 200 nM 4′,6-diamidino-2-phenylindole (DAPI) ...
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bioRxiv - Microbiology 2022Quote: ... Coverslips were washed ten times in PBS and ten times in ultrapure water before mounting on slides using Mowiol 4–88 (Merck) containing 200 nM 4′,6-diamidino-2- phenylindole (DAPI) ...
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bioRxiv - Microbiology 2022Quote: ... 10 times using Amicon centrifugal filter units (Merck Millipore) with a 100 kDa cut-off to a final volume of 5 mL ...
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bioRxiv - Microbiology 2022Quote: ... 10 times using Amicon centrifugal filter units (Merck Millipore) with a 100 kDa cut-off to a final volume of 20 mL ...
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bioRxiv - Microbiology 2023Quote: ... three times using 100 kDa amicon filters (Merck, Millipore) and centrifuging at 3’000 × g for 6 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein pellets were washed five times with cold Acetone (Merck) and submitted for subsequent mass spectrometric analyses by the Functional Genomics Center Zurich (FGCZ) ...
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bioRxiv - Bioengineering 2019Quote: ... The NPs were washed three times in ethanol (99 % absolute, Merck) and three times in deionized water ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were washed three times with 1X PBS (Merck, D8537) to remove excess mucus ...
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bioRxiv - Cell Biology 2020Quote: ... and subsequently washed 4 times in Quencher solution (5 mM Trolox (Merck), 10 mM Na-Ascorbate (Merck)) ...
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bioRxiv - Developmental Biology 2022Quote: ... and then three times with 100 mM ammonium acetate (Merck, cat. # 1.01116.1000). Embryos were deposited in single spots on a microscope slide and desiccated for one hour in a vacuum chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... Buffers were filtered two times (0.22 μm pore size filter units, Merck) and degassed for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were washed two times in 50 % formamide (Emsure, ref 1.09684.1000, Merck), 5x saline sodium citrate (SSC ...
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bioRxiv - Microbiology 2022Quote: ... was diluted 100 times in 0.22 μm-filtered DMSO (IC Millex, Merck, USA) and Propidium Iodide (20 mM in dimethyl sulfoxide (DMSO) ...
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bioRxiv - Cell Biology 2022Quote: ... washed three times with PBS and permeabilized with 0.1% Triton X-100 (Merck) in PBS for 5 min ...
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bioRxiv - Molecular Biology 2019Quote: ... 40 times by centrifugal filtration (3K Amicon Ultra, Merck Millipore, 14’000g, 4°C) and buffer was exchanged to 4M Guanidine hydrochloride in 50mM HEPES (pH 7.8) ...
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bioRxiv - Developmental Biology 2019Quote: ... washed again 3 times 15 min and developed using Millipore Crescendo ECL (Merck). For western blot quantification ...
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bioRxiv - Developmental Biology 2021Quote: ... washed 3 times in PBS with 1x cOmplete Protease Inhibitor Cocktail (Merck, 11697498001).
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bioRxiv - Immunology 2020Quote: ... by filtrating four times over 100 kDa cut-off cellulose centrifugal filter (Merck).
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bioRxiv - Microbiology 2023Quote: ... Cells were washed four times with PBS and lysed with 2% saponin (Merck) for 30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... slides were washed with deionized water three times and counterstained with Mayer’s hematoxylin (Merck). Slides were then rinsed with deionized water for 5 minutes and mounted with a coverslip using Aquatex mounting medium ...
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bioRxiv - Neuroscience 2022Quote: ... slices were washed two times with 1X Phosphate Buffer (30 mM Na2HPO4·12H2O [Merck, 10039-32-4] ...
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bioRxiv - Developmental Biology 2022Quote: ... and detergent were removed by washing five times with 8M Urea (Merck, Darmstadt, Germany) 50mM Tris (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... Wells were washed 3 times with 200 µL PBS containing 0.1% Tween-20 (Merck) (PBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were washed three times with PBS and mounted in Moviol 4-88 (Merck). Wide-field fluorescence microscope Leica (Leica ...
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bioRxiv - Molecular Biology 2020Quote: ... Fresh fecal samples were diluted with nine times buffered peptone water (BPW) (Merck, Germany) and 25 g of sample was added to 225 mL of BPW pre-enrichment media ...
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bioRxiv - Cell Biology 2022Quote: ... passed 10 times though a 27 G needle and laid on an Optiprep (Merck) gradient ...
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bioRxiv - Developmental Biology 2023Quote: ... and detergent were removed by washing five times with 8M Urea (Merck, Darmstadt, Germany) 50mM Tris (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... We dipped shoots 20 times in a 0.1% Tween 20 solution (Merck, NJ, USA) followed by 20 rinses with milli-Q water to remove surface trace element contamination (Schmidt et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... slices were washed three times with DMEM-F12 and grown on Millicell culture inserts (Merck) in cortical culture medium (DMEM-F12 containing B27 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then washed three times in PBS and counterstained with Hoechst 33342 (Merck, B2261). F-actin was detected using Alexa Fluor 488-conjugated phalloidin (ActinGreen 488 ReadyProbes ...
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bioRxiv - Cell Biology 2021Quote: ... After treatment for the indicated time the cells were fixed in 4% paraformaldehyde (Merck, 100496,) and permeabilized with cold methanol at −20°C for 2 min or 0.1% TX-100 in PBS for 5 min at room temperature followed by blocking with 0.12% glycine (Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... washed four times in TBS+T and signals revealed by enhanced chemiluminescence (Merck Cat#WBLUF0100) on a ChemiDoc Touch Imaging System (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... cells and tissue were washed 3 times with phosphate buffered saline (PBS) + 0.02M glycine (Merck, K27662101) to remove fixative ...
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bioRxiv - Neuroscience 2021Quote: ... Stained sections were washed 4 times in PBS and then embedded using Aquatex (Merck Millipore, USA).
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bioRxiv - Microbiology 2020Quote: ... were confirmed by comparison of retention time to a commercial reference of (+)-valencene purchased (75056, Merck). To estimate the titre of α-cuprenene ...
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bioRxiv - Developmental Biology 2022Quote: ... The beads were then washed five times with binding buffer with 0.05% IGEPAL CA630 (Sigma, Merck). One tenth of the beads were eluted in loading buffer for Western blot purposes and the rest was processed for Mass Spectrometry analysis.
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bioRxiv - Biophysics 2020Quote: ... the mixture was washed with PBS buffer four times using 10kD cut-off concentrators (Merck-millipore). Labelled bacterial MVs were kept at 4°C and can be stored upto one week for further experiments.
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bioRxiv - Biochemistry 2021Quote: ... After binding beads were washed 3 times with binding buffer (PBS with protease inhibitor cocktail (Merck) and 1 mM Na3VO4 ...
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bioRxiv - Cell Biology 2021Quote: ... washed four times with PBS and permeabilized for 5 min with 0,5% Triton X-100 (Merck) in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed three more times and affixed to slides with Fluoroshield histology mounting medium (Merck). Cell were viewed on am IX83 inverted microscope (Olympus ...
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bioRxiv - Cell Biology 2023Quote: ... all materials and equipment used were rinsed three times with filtered (0.1 μm filter – Merck Isopore) ultra-pure water prior to use ...
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bioRxiv - Developmental Biology 2023Quote: ... all materials and equipment used were rinsed three times with filtered (0.1 μm filter – Merck Isopore) ultra-pure water prior to use ...