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Citations for Merck :
1 - 50 of 1341 citations for RapidClean Protein Removal kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... samples (100 µl culture broth) were subjected to protein precipitation followed by phospholipid removal (HybridSPE, Merck/Sigma Aldrich). The internal standard E2-d5 (Chromsystems ...
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bioRxiv - Plant Biology 2020Quote: ... After removal of debris with a Miracloth (Merck), chromatin was dissolved into ChIP dilution buffer and sonication was conducted by S2 sonicator (Covaris) ...
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bioRxiv - Biophysics 2020Quote: ... After removal of the poly-E tag with the 3C protease (MERCK), the protein was again subjected to gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentrations were determined with the BCA protein assay kit (Novagen, Merck KGaA), and protein homogeneity was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cancer Biology 2021Quote: Intracellular protein was extracted from cells using the NucBuster Protein Extraction Kit (Merck) and quantified using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was estimated using BCA protein assay kit (Merck, 71285-3, Germany).
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bioRxiv - Neuroscience 2021Quote: ... protein concentration was quantified using a Sigma-Aldrich BCA protein assay kit (Merck). Subsequently ...
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bioRxiv - Physiology 2020Quote: ... Total proteins from the supernatant were quantified using a Bradford Protein Assay Kit (Merck) and subjected to electrophoresis on 15% SDS–PAGE gels ...
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bioRxiv - Physiology 2022Quote: ... Protein carbonylation was assessed with an Oxyblot protein oxidation detection kit (#S7150, Merck Millipore) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... followed by removal of excess staples with 100 kDa MWCO Amicon centrifugal filters (Merck). For the NSTR and NSTR/ K-PEG designs ...
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bioRxiv - Molecular Biology 2022Quote: Millipore Compartment Protein Extraction Kit (Merck-Millipore, Cat. #2145) was used to deplete cytosolic ...
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bioRxiv - Physiology 2022Quote: ... To detect total protein carbonylation by immunoblot we used the OxyBlot Protein Oxidation Detection kit (Merck) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore ...
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bioRxiv - Cell Biology 2023Quote: Protein carbonyl formation by oxidative reactions was assessed with the OxyBlot Protein Oxidation Detection Kit (S7150, Merck Millipore), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... After removal of the poly-E tag located on the “alive” subunit with 3C protease (MERCK) followed by a gel filtration step (Superdex-200 10/300 GE Healthcare Life Sciences) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... For removal of chorion embryos were incubated for 5 mins with 1 mg/ml Pronase (Merck) then washed with culture medium and flash-frozen in liquid nitrogen ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... Soluble proteins were then dosed using the Bicinchoninic Acid Kit (Merck) and diluted in Laemmli protein sample buffer ...
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bioRxiv - Immunology 2022Quote: ... The protein concentration of ES antigen was estimated using protein estimation kit by Bradford method (Merck GeNei™, Bangalore).
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bioRxiv - Pharmacology and Toxicology 2020Quote: The eluted corona proteins from nanoparticles were first precipitated by using the ProteoExtract® Protein Precipitation Kit (Merck, Germany) as described in the manufacturer protocol ...
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bioRxiv - Microbiology 2022Quote: ... Labelling began with the removal of PBS and addition of 0.1% Triton X-100 (Merck Life Sciences) for approximately 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... After removal of TCEP via 10 kDa molecular weight cut-off (MWCO) Amicon spin filters (Merck, #UFC500324), the reduced nanobodies were coupled using 10-fold excess of maleimide-DBCO crosslinker (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: The Magna RNA-binding protein immunoprecipitation kit (Merck Millipore, Billerica, MA, USA) was applied for the assay ...
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bioRxiv - Cell Biology 2021Quote: Co-IP was performed using Protein G Immunoprecipitation Kit (Merck, Cat # IP50) as per the manufacturer's protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Delipidated FBS was prepared by incubating FBS with 0.5 g/ml lipid removal beads (13358-U, Sigma/Merck) at 4°C overnight with rotation followed by centrifugation (27,000g ...
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bioRxiv - Cell Biology 2023Quote: Saporin was labelled with an ATTO 550 protein labelling kit (Merck, 51146-1KT).
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bioRxiv - Bioengineering 2019Quote: ... Removal of excess prongs was done first with washing in 50 kDa MWCO 0.5 mL Amicon centrifugal filters (Merck Millipore) and then by means of Streptavidin-coated magnetic beads (DYNAL MyOne Dynabeads Streptavidin C1-Thermofisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... the sample was placed under vacuum for complete removal of the acetone and resuspended with 100 uL of 100 mM HEPES buffer (Merck).
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bioRxiv - Cell Biology 2020Quote: ... and nucleoproteins were extracted using a nuclear protein extraction kit (Merck Millipore, Darmstadt, Germany). Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS ...
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bioRxiv - Cancer Biology 2022Quote: RIP was performed according to the Magna-RIPTM RNA-binding protein immunoprecipitation kit (Millipore/Merck) as previously described (Papoutsoglou et al ...
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bioRxiv - Molecular Biology 2022Quote: The concentration of protein extracts was determined by BCA assay kit with BSA standards (Merck).
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bioRxiv - Biochemistry 2022Quote: ... Cell lysis and membrane protein extraction was performed using the ProteoExtract Native Membrane kit (Merck Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Purified proteins and native protein standard (Supelco-Merck) were loaded in 4-15% gradient mini protean precast native gel (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: Ribonucleoprotein complex immunoprecipitation (RIP) was performed using the EZ-Magna RIP RNA-binding protein Immunoprecipitation Kit (Merck Millipore Billerica ...
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bioRxiv - Microbiology 2019Quote: Supernatants of ham samples were clarified to eliminate proteins using the Carrez clarification kit (Merck KGaA, Darmstadt, Germany). Proteins were precipitated via the successive addition of 600 µL of Carrez reagent I and 600 µL of Carrez reagent II to 5 mL of supernatant ...
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bioRxiv - Molecular Biology 2022Quote: ... and SmWRKY27-GST were purified using a NucBuster™ Protein Extraction Kit (Cat. #71183; Merck KGaA, Darmstadt, Germany) for use in further EMSA experiments ...
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bioRxiv - Cancer Biology 2022Quote: An RIP assay was performed using a Magna RIP RNA binding protein immunoprecipitation kit (17-700; Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Total protein concentration of lysates was determined using a bicinchoninic acid kit (Merck Life Science UK Ltd, Gillingham, UK) and samples diluted in Laemmli buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Protein A and Protein G conjugated to HRP were purchased from Merck. DNA dye Hoechst 33342 was purchased from ThermoFisher Scientific and used at a final concentration of 5 μg/ml ...
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bioRxiv - Immunology 2020Quote: ... Thirty µl of Protein G (Protein G Sepharose Fast Flow, Merck, UK) slurry per sample was added in addition to 0.01% sodium azide and incubated on a roller overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Total protein concentration was quantified by means of BCA protein assay (Merck). 10-20 μg of protein was separated by size using a 4-12% Bis-Tris Plus gel ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Protein A and Protein G conjugated to HRP were purchased from Merck. DNA dye Hoechst 33342 was purchased from ThermoFisher Scientific and used at a final concentration of 5 µg/ml.
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bioRxiv - Cell Biology 2022Quote: ... The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck, Poznan, Polska). The samples containing 20 µg of protein were mixed with 4× Laemmli loading buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... RIP-Seq experiments with UV cross-linking were performed using the Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit (17-700 Merck). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... The clear cell-free extracts were immunoprecipitated using monoclonal antibodies against T18 tag following Protein G Immunoprecipitation Kit (Merck Inc) protocol ...
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bioRxiv - Immunology 2022Quote: ... an aliquot of the sample was taken for measuring the total protein concentration using a BCA assay kit (Merck Millipore). The remaining sample was heat-treated (70 °C ...
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bioRxiv - Plant Biology 2024Quote: ... Calibration of the column was performed using the Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da (#MWGF200; Sigma-Aldrich/Merck, www.sigmaaldrich.com).
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bioRxiv - Molecular Biology 2020Quote: ... From the remaining nuclei RNA-IP was then undertaken with the MagnaRIP Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit protocol (17-700, Merck) from step I.3 ...
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bioRxiv - Plant Biology 2022Quote: The m6A enrichment experiment was performed as described previously with minor modifications (Dominissini et al., 2013) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck) was used following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... 500 μg of protein were added to washed agarose protein A beads (Merck Millipore) and incubated on a rotating wheel for 1 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... protein standards (Merck Sigma-Aldrich) specified in the Supplementary Material were separated on the same column.