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Citations for Merck :
1 - 50 of 1403 citations for Precast Protein Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... or mPAGE 4-12% Bis-Tris Precast gels (#MP41G10, Merck). Separated proteins were then transferred to PVDF or Nitrocellulose membranes according to manufacturer’s instructions (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... and lysed in SDS sample buffer containing 100 mM DTT before protein separation on precast Bis-Tris polyacrylamide gels (MPAGE; Merck) and transferred to nitrocellulose membranes by electroblotting ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... on a 4-20% precast Trupage gel (#PCG2012-10EA; Sigma-Aldrich/Merck). For Western blotting ...
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bioRxiv - Molecular Biology 2023Quote: ... separated using mPAGE 4-12% Bis-Tris precast gels (Merck Millipore, MP81G15) in mPAGE MOPS SDS Running Buffer (Merck Millipore ...
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LptM promotes oxidative maturation of the lipopolysaccharide translocon by substrate binding mimicrybioRxiv - Microbiology 2023Quote: ... protein gels were blotted onto PVDF membranes (Merck). Upon blocking with skim milk ...
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bioRxiv - Cell Biology 2023Quote: ... except that SDS-PAGE was carried out using Millipore mPAGE® 10% Bis-Tris Precast Gels (MP10W10/ MP10W12/ MP10W15) with MES SDS running buffer (MPMES) from Merck, before protein transfer to PVDF membranes.
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bioRxiv - Plant Biology 2020Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Plant Biology 2019Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were transferred from the gel to a PVDF membrane (Merck, IPFL00010) by semi-dry transfer with Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Bound proteins were analysed by mPAGETM 4-20% Bis-Tris gels (Merck) and Coomassie staining ...
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bioRxiv - Developmental Biology 2024Quote: ... Following gel-electrophoresis proteins were transferred to a PVDF membrane (Merck, IPVH00010) using a semi-dry transfer cell (Biorad Transblot SD) ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... were electrophoresed on SDS polyacrylamide gels ( NuPAGE™ 4-12% Bis-Tris Protein Gels; InvitrogenTM) and transferred to PVDF membranes (Millipore; Merck). Membranes were blocked in 2.5% (w/v ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins separated in the gel were transferred to a PVDF membrane (Merck Millipore) using a submerged blotting apparatus and then visualized using Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...
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bioRxiv - Cancer Biology 2019Quote: ... proteins were transferred from gel to a PVDF membrane (Merck, cat. no. IPSN07852). Transfer was carried out for 45 minutes at 110V in transfer buffer (cold room) ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were resolved in SDS-PAGE gels and electrotransferred onto nitrocellulose membranes (Merck). After incubation with the appropriated primary antibody ...
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bioRxiv - Pathology 2021Quote: ... proteins were transferred from the gel onto to a polyvinylidene fluoride membrane (Merck Millipore) via electroblotting at 120 V for 60 minutes in transfer buffer containing 20% methanol ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were separated on SDS/PAGE gels and transferred onto a PVDF membrane (Merck). After blocking the membrane with 5% fat-free milk in TBST ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were transferred from the gels to Immobilon PVDF membranes (Merck Millipore Ltd.). The membranes were immersed in appropriate blocking buffer (Tris-buffered saline 0.05% Tween 20 (TBST ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were resolved in SDS-polyacrylamide gels and blotted onto PVDF membranes (Millipore/Merck) or nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... NuPage 4 to 12% bis-tris protein gels (NP0323, Thermo Scientific; or MP42G15; Merck) before transferring into 0.22μm pore size polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2024Quote: ... For protein reduction gel cubes were mixed with freshly prepared 10mM DTT (dithiothreitol; Merck) in 50mM BA and incubated 1 h at 56C ...
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bioRxiv - Developmental Biology 2020Quote: ... Fifty or thirty-five (depending on the protein detection) micrograms of proteins was separated on 9% Tricine gels and transferred to polyvinylidene difluoride membranes (Merck Millipore). The membranes were incubated separately with anti-Mmp-9 (1:1000 ...
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bioRxiv - Plant Biology 2023Quote: ... The separated total proteins or proteins from two-dimensional gels were subsequently transferred onto polyvinylidene difluoride (PVDF) membranes (Merck, Cat. IPVH00010), hybridized with specific antibodies ...
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bioRxiv - Genetics 2022Quote: ... 50 μg of protein was used for gel electrophoresis in 10-14% SDS-PAGE gels and transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA). After blocking in 5% defatted milk ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred from the polyacrylamide gel to a PVDF membrane (Merck Millipore, Massachusetts, USA). Membranes were blocked with Rockland blocking buffer (Biomol GmbH ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins in the gel were then transferred to a poly-1,1-difluoroethane (PVDF) membrane (Merck) in transfer buffer (25 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... Proteins were resolved on SDS–PAGE gels and transferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore). The PVDF membranes were then blocked with 5% skim milk and sequentially incubated with primary and secondary antibodies ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... proteins were separated with SDS-PAGE gels and transferred to PVDF membranes (Immobilon-P, Merck Millipore, USA). Blotted membranes were reacted with the specific primary antibodies shown in Supplementary Table 1 followed by peroxidase-linked secondary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... SDS-PAGE gels were used for protein separation followed by transfer onto PVDF membrane (Merck, Cat. # IPFL00010). Blots were blocked with 5% BSA at room temperature for 1 h and incubated with the following primary antibodies overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Equal quantities of protein were separated on SDS-PAGE gels and transferred to the PVDF membranes (Merck), which were then blocked with 1 % nonfat skim milk in TBS-T for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were then separated in 12% gel by SDS-PAGE and transferred to PVDF membranes (Merck Millipore, ISEQ00010), which were then block with 5% milk in TBS-T buffer at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were separated by SDS-PAGE gel electrophoresis and then transferred to the PVDF membrane (Merck, USA).
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were separated on an SDS-PAGE gel and then transferred to a nitrocellulose (NC) membrane (Merck Millipore, ISEQ00010), which was incubated with individual protein-specific primary antibodies at 4 °C overnight on a shaker ...
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bioRxiv - Biochemistry 2020Quote: Proteins separated on PAGE gel were transferred to a PVDF membrane (Immobilon-P/Immobilon-FL, 0.45 μm, Merck Millipore) using Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... proteins were separated using a 10% SDS-PAGE gel and transferred onto a PVDF Immobilon-P membrane (Merck Millipore). The membrane was blocked with 5% w/v milk in TBST (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Cell Biology 2023Quote: ... Separated proteins on the gels were blotted on Amersham™ Hybond® low-fluorescence 0.2 µm PVDF membranes (Merck). The membranes were blocked using fluorescence blocking buffer (Azure Biosystems ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were then transferred from the gel to a PVDF membrane (Immonilon-P transfer membrane, Merck, Darmstadt, Germany) using the wet transfer method ...
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bioRxiv - Genetics 2021Quote: ... For western blotting the proteins were resolved in SDS-polyacrylamide gels and transferred electrophoretically at room temperature to PVDF membranes (Merck) for 1 h at 50 mA using a Tris-glycine buffer system ...
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bioRxiv - Molecular Biology 2020Quote: ... 30~50 μg of protein samples were separated on a 10% SDS-PAGE gel and transferred to a PVDF membrane (Merck) using an Amersham semi-dry transfer system ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were loaded and resolved using a 10% SDS-PAGE gel and the separated proteins were transferred onto a PVDF membrane (IPVH00010, Immobilon-P; Merck). Blocking was performed using 5% Skimmed milk (70166 ...
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bioRxiv - Cancer Biology 2019Quote: ... Subsequently 20 μg of protein were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a polyvinylidene difluoride membranes (Immobilon-P PVDF, EMD, Merck,). Membranes were blocked with 5% fat-free milk in TBST (0.01% Tween ...
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bioRxiv - Immunology 2020Quote: ... The proteins were then separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto an Immobilon-P membrane (IPVH00010; Merck). The membranes were blocked with 10% nonfat milk (190-12865 ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were separated in a 10% SDS-polyacrylamide gel and transferred to Immobilon NC membranes (Millipore Merck, Darmstadt, Germany). The membranes were blocked with 5% non-fat dry milk in PBS for 1 h at room temperature and then incubated with primary antibodies (DB 1:2000 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated on a 10% polyacrylamide gel and transferred to polyvinylidene difluoride membrane (Amershan Hybond P PVDF membrane, Merck). Membranes were blocked in 5% milk in PBS/0.1% Tween-20 ...
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bioRxiv - Physiology 2023Quote: ... and 20 µg protein extracts were separated with 10-15% SDS-PAGE gel and transferred to the PVDF membranes (Millipore ISEQ00010, Merck). Each membrane was blocked and slit into two parts ...
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bioRxiv - Cell Biology 2023Quote: Whole liver lysates and lipid droplet-enriched fraction proteins were loaded on 10% SDS-PAGE gels and transferred onto polyvinylidene fluoride membranes (Merck). Membranes were incubated at room temperature for 1 h with blocking buffer (TBS ...
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bioRxiv - Plant Biology 2024Quote: ... Calibration of the column was performed using the Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da (#MWGF200; Sigma-Aldrich/Merck, www.sigmaaldrich.com).
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bioRxiv - Immunology 2021Quote: ... Samples of the eluted fractions were loaded into 15% SDS-PAGE gels and the fractions containing the nucleocapsid protein were concentrated using Amicon Ultra-15 concentric filters (Merck Millipore) with a 10 kDa cutoff ...
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bioRxiv - Biochemistry 2022Quote: ... were separated on 0.1% SDS polyacrylamide gels (6–12% w/v depending on the molecular mass of protein) and electroblotted onto Immobilon-PVDF Transfer Membrane (Merck Millipore). Blots were blocked in 5% w/v non-fat milk or 5% BSA (Albumin ...