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Citations for Merck :
1 - 50 of 301 citations for PD L1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... FITC conjugated goat anti-human IgG Fc was from Merck, Dorset ...
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bioRxiv - Cell Biology 2024Quote: Human embryonic kidney (HEK293) cells were cultured in Dulbeccoʹs Modified Eagleʹs Medium (DMEM, Merck) containing 10% Fetal Calf Serum and 1% Antibiotic Antimycotic Solution (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... and PD: PD0325901 (all Merck) were solved in DMSO at stock concentrations of 20 mM.
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bioRxiv - Molecular Biology 2021Quote: ... and goat anti-Human IgG (Fc specific)-Cy3 (1:1000 dilution in binding buffer; Merck). Finally ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μM PD 0325901 (Merck #PZ0162), LIF (Merck # ESG1107) ...
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bioRxiv - Plant Biology 2021Quote: ... desalted with PD 10 desalting columns (Merck) in extraction buffer (50 mM HEPES with 1mM EDTA ...
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bioRxiv - Bioengineering 2022Quote: ... desalted using a PD-10 desalting column (Merck), and loaded on a preparative Superdex200 increase 10/300 GL (GE Healthcare ...
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bioRxiv - Developmental Biology 2023Quote: ... ERK antagonist PD 0325901 (1uM, PZ0162-5MG, Merck) and 1000 units/ml LIF diluted in N2B27 ...
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bioRxiv - Cancer Biology 2023Quote: ... T cell medium (human serum was replaced with FCS during thawing) and incubated for 15 minutes with 25U/mL benzonase (Merck). After washing ...
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bioRxiv - Microbiology 2021Quote: ... Purified proteins were desalted using a PD-10 column (Merck), concentrated using an Amicon Ultra-4 10 kDa cut-off spin column (Merck) ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.5 with PD 10 columns (Merck, New Jersey, USA) using the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: HEK293 and NIH-3T3 cells were cultured in DMEM (Merck) supplemented with 10% FBS (Merck) ...
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bioRxiv - Immunology 2019Quote: ... Fc IgG (AG714, Merck Millipore) at 2 μg/ml in 150 μl of PBS for one hour ...
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bioRxiv - Biophysics 2019Quote: ... supplemented with 10 % FCS (Merck), 100 µg ml−1 penicillin (Life technologies) ...
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bioRxiv - Biophysics 2019Quote: ... supplemented with 10% FCS (Merck), 100 µg ml−1 penicillin (Life technologies) ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with 10% FCS (MERCK), 100 μg ml−1 penicillin (Life technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with 10% FCS (Merck) and 2 mM L-glutamine (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... + 10% FCS (Merck Millipore S0615) + 1% P/S (Thermo Fisher #15140122) ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% FCS (Merck), 2mM L-Glutamine (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... whereas HEK293 cells were cultured in DMEM (D5796 MERCK, Darmstadt, Germany) containing 10% (vol./vol. ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Merck) supplemented with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... L1s were then broken up to extract proteins using a Beadbug homogenizer (Merck Life Science) at the highest setting for 90 seconds three times at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10% FCS (Merck KGA, Germany), 4 mM glutamine (Merck KGA ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were incubated with 50 µM 17ODYA (17-octadecynoic acid, Merck) added from stock solution in DMSO or with 0.05% DMSO in DMEM containing 2% charcoal-stripped FBS and 30 mM HEPES for 4 h at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Eluted protein was desalted using PD-10 desalting columns (Sigma-Merck, U.S.A/Germany). Concentration of the desalted protein was estimated using Bradford’s method and the protein was stored at -80 °C until needed.
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: HEK293 cells were cultured in Dulbecco⍰s Modified Eagle⍰s Medium (DMEM, Merck) containing 10% Fetal Calf Serum and 1% Antibiotic Antimycotic Solution (Sigma Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... and 10% Fetal calf serum (FCS) (Merck). Both cell lines were cultivated in T75 culture flasks ...
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bioRxiv - Systems Biology 2020Quote: FGF receptor inhibitor (FGFRi) stock: PD 173074 (#P2499-5MG, Merck & Co., Inc., NJ, USA) diluted in DMSO (#D2650-5×5ML ...
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bioRxiv - Biophysics 2019Quote: ... and 10 ml RPMI containing 0.75 % FCS (Merck) in a common 50-ml Eppendorf tube and heated up to 68°C using a common water bath ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorinated oil (FC- 40, Merck, Kenilworth, NJ, USA) was used to remove the excess amounts of the reaction solution and to seal the reactor (Supplementary Fig ...
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bioRxiv - Bioengineering 2023Quote: ... FCS was mixed with 50% PEG-10,000 (Merck) at a 5:1 ratio and rotated at 4 °C for 3 h ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells seeded in 6-well plates or 100mm dishes were transfected using GeneJuice (Merck) and 1 to 3µg DNA ...
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bioRxiv - Neuroscience 2024Quote: ... we used fixed HEK293 cells labeled with primary antibody (rabbit anti-GM130, 1:1000, Merck) and secondary antibody (goat anti-rabbit conjugated with Alexa Fluor 555 ...
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bioRxiv - Genomics 2020Quote: ... supplemented with 15% fetal calf serum (FCS) (Merck Millipore), 0.1mM b-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... was supplemented with 10% fetal calf serum (FCS) (Merck), 1x non-essential amino acids (NEAA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... supplemented with 10% foetal calf serum (FCS, Merck, Germany). Once confluent ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with 10% Fetal Calf Serum (FCS, #S0615, Merck), 4 mM L-glutamine (#25030-024 ...
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bioRxiv - Cell Biology 2023Quote: ... and anti-mouse IgG (Fc) TRITC (Merck, #SAB3701020, 1:500).
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bioRxiv - Cancer Biology 2021Quote: ... cultures were treated with 4 μM of the CDK4/6 inhibitor Palbociclib/PD-0332991 (Merck, PZ-0199) for 8 days ...
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bioRxiv - Bioengineering 2024Quote: ... Unlabeled AAVs were detected on HEK293 cells post fixation by anti-AAV9 clone HL2372 (Merck, MABF2309-100UL) at a 1:500 dilution followed by AF647-labeled anti mIgG H+L.
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bioRxiv - Biochemistry 2019Quote: ... 5 was performed using 600 μL HEK293 cells (±CL) previously treated with 2000 U/mL Benzonase (Merck, 70664) in the recommended buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: hTERT RPE-1 and HEK293(T) cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM/F12; Merck) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... 100 μL of anti-mouse IgG (Fc-specific)−biotin (Merck B7401) were added and incubated for 45 min ...
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bioRxiv - Biophysics 2022Quote: ... containing 10mM HEPES (Roth) and 0.5% FCS (Biochrom GmbH, Merck Millipore). One chamber slide was used for gel expansion and the other one as non-expanded control ...
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bioRxiv - Biophysics 2023Quote: ... with the addition of 10% FCS (fetal calf serum, #S0615, Merck) and 4 mM glutamine (#25030-024 ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% FCS (Bio & Sell) and 1% Penicilin/Streptomycin (Merck). Cells were grown in a humidified incubator at 37°C with 5% CO2 and regularly tested for mycoplasma contamination ...
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bioRxiv - Physiology 2019Quote: ... human tubal fluid (HTF) medium and human serum albumin (HSA) (Merck Millipore Corporation ...
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bioRxiv - Biochemistry 2022Quote: The protein stock solutions were transferred to a deuterated buffer containing 35 mM potassium phosphate and 25 mM NaCl in D2O at a pD of 7.5 using 0.5 mL Centricon devices (Merck Millipore) with a MWCO of 30 kDa by washing and centrifuging several times at 10,000 x g and 4°C ...
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bioRxiv - Cell Biology 2022Quote: The following inhibitors were used in this study: Palbociclib (PD-0332991, hydrochloride salt, MedChemEx-press, HY-50767A, Merck PZ0383); Rapamycin (MedChemExpress ...