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Citations for Merck :
1 - 50 of 913 citations for Nucleic Acid Electrophoresis Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... integrity was inspected on 1% agarose gel in TAE buffer pre-stained with GelRed nucleic acid stain (Merck).
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bioRxiv - Microbiology 2023Quote: ... Nucleic acids were labelled with 3 µM DAPI (Merck, Belgium) for 20 minutes ...
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bioRxiv - Synthetic Biology 2020Quote: ... nucleic acids were digested by addition of 1 µL benzonase (Merck KGaA) and 10 µL 1 M MgSO4 followed by incubation at room temperature for 10 min ...
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bioRxiv - Genomics 2020Quote: ... Free nucleic acids were then digested with 60 units/ml Benzonase Nuclease (Merck) for at least 30 minutes at 37°C ...
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bioRxiv - Microbiology 2023Quote: SDS gel electrophoresis was performed using pre-cast mPAGE™ 4-20% bis-tris gels (Merck) and 20 µl of PS extracts were mixed in Laemmli buffer and boiled at 95°C for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA:DNA hybrids were immunoprecipitated from total nucleic acids using 5µg of S9.6 antibody (MABE1095, Merck Millipore) in binding buffer (10mM Na2HPO4 pH 7.0 ...
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bioRxiv - Microbiology 2021Quote: ... polyacrylamide gel electrophoresis and general laboratory reagents were from Merck (Moscow, Russia).
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bioRxiv - Developmental Biology 2024Quote: ... Following gel-electrophoresis proteins were transferred to a PVDF membrane (Merck, IPVH00010) using a semi-dry transfer cell (Biorad Transblot SD) ...
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bioRxiv - Neuroscience 2023Quote: ... Targeting proteins were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) by electrophoresis and transferred onto a polyvinylidene fluoride (PVDF) film (Merck Millipore, Guangzhou, China). The PVDF membrane carrying proteins was blocked by 5% fat-free milk dissolved in 1× TBST (0.1% Tween20 in Tris-buffered saline ...
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bioRxiv - Immunology 2019Quote: ... Cell lysates were separated by SDS-gel electrophoresis and transferred to PVDF membranes (Merck Millipore). Immunoblotting was performed using the following antibodies from Cell signaling ...
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bioRxiv - Immunology 2024Quote: ... The ethanol and unloaded nucleic acids in the mixtures were removed using Amicon Ultra centrifugal filters (10- or 30-kD MWCO, Merck) for two rounds with 5- and 15-times volumes of 1× PBS ...
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bioRxiv - Biophysics 2022Quote: ... three washes in PBS+10%HS each lasting 5min and staining of nucleic acids with Hoechst 33342 (5μg/mL-Merck Bio-sciences) for 5min ...
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bioRxiv - Genetics 2022Quote: ... 50 μg of protein was used for gel electrophoresis in 10-14% SDS-PAGE gels and transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA). After blocking in 5% defatted milk ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins in 65 μl of conditioned media and cell lysates per lane were separated on 6% polyacrylamide gels using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred on a poly-vinylidene difluoride (PVDF) membrane (Immobilon-FL, Merck Millipore Ltd., Burlington, MA) using the MiniProteas Blotting Module (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to an AmershamTM HybondTM 0.45µm polyvinylidene difluoride (PVDF) membrane (Merck). CITRINE-fusion proteins were detected with anti-GFP antibody (JL-8 ...
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bioRxiv - Developmental Biology 2021Quote: ... resolved by SDS-polyacrylamide gel electrophoresis and subsequently transferred to nitrocellulose membranes (Amersham Protran Western Blotting Membranes; Merck). The filters were blocked with TBS containing 0.05% Tween and 5% non-fat dry milk (Merck) ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were separated by SDS-PAGE gel electrophoresis and then transferred to the PVDF membrane (Merck, USA).
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bioRxiv - Neuroscience 2022Quote: ... 25 μg of protein were resolved by 8% dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then electrophoretically transferred to a 0.45 μm polyvinylidene difluoride membrane (PVDF; Immobilon, Merck Millipore). Membranes were blocked in blocking buffer (5% non-fat milk or 5% FBS diluted in TBS-T buffer (0.05% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2019Quote: ... Subsequently 20 μg of protein were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a polyvinylidene difluoride membranes (Immobilon-P PVDF, EMD, Merck,). Membranes were blocked with 5% fat-free milk in TBST (0.01% Tween ...
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bioRxiv - Molecular Biology 2020Quote: ... The prepared samples were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a polyvinylidene fluoride (PVDF) membrane (Merck Millipore). The PVDF membrane was blocked in 5% skimmed milk in Tris-buffered saline containing Tween for 1 hour and incubated with primary antibodies overnight at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... The proteins were then separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto an Immobilon-P membrane (IPVH00010; Merck). The membranes were blocked with 10% nonfat milk (190-12865 ...
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bioRxiv - Biochemistry 2023Quote: ... The prepared samples were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a polyvinylidene fluoride (PVDF) membrane (Merck Millipore). The PVDF membrane was blocked in 5% skimmed milk in Tris-buffered saline containing Tween for 1 hour and incubated with primary antibodies overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were separated by SDS–polyacrylamide gel electrophoresis and then transferred to a polyvinylidene difluoride (PVDF) membrane (Hybond-P PVDF membranes, Merck, Germany) by a wet-transfer apparatus (Vep-3 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto 45-μm polyvinylidene difluoride (PVDF) membrane (IPVH00010, Merck Millipore). Membranes were blocked using 5% non-fat dry milk in Tris-buffered saline with 0.1% Tween 20 (655204 ...
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bioRxiv - Immunology 2022Quote: ... resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with Laemmli running buffer and transferred to PVDF membranes (Merck-Millipore) in a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... 20 µg of protein was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto Immobilon®-P PVDF membrane (Merck), followed by blocking in milk and further overnight probing at 4 °C with a primary antibody specific for Cre (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membrane (PVDF Transfer Membrane, Merck-Millipore). The primary antibodies used for the detection of proteins are Anti-CRY1 (Bethyl) ...
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bioRxiv - Cell Biology 2024Quote: ... The cell lysate was then separated by electrophoresis in a 10% polyacrylamide gel and transferred to a PVDF membrane (Merck Millipore). The membrane was blocked with 5% milk in PBS-T ...
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bioRxiv - Microbiology 2021Quote: ... A total of 20 µg of protein from each sample was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to the polyvinylidene difluoride (PVDF) membrane (Merck Millipore, USA). Then ...
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bioRxiv - Developmental Biology 2022Quote: ... separated by polyacrylamide gel electrophoresis and then transferred to either nitrocellulose or a polyvinylidene difluoride (PVDF) (Merck Life Science UK Ltd, UK). Membranes were then processed for Western blotting using the antibodies listed in Supplementary Table 5 ...
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bioRxiv - Molecular Biology 2023Quote: ... and loaded in 4–12% NuPAGE Bis-Tris gel (Fisher, 10247002) for subsequent electrophoresis and transference to nitrocellulose membrane (Merck-Sigma, GE10600003). Precipitated RNA was analyzed by autoradiography and FLAG-PTBP protein levels were measured by western blot.
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bioRxiv - Plant Biology 2023Quote: ... Total proteins were separated by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene fluoride membranes (Merck Millipore, USA). OsJMT1-HA was detected by immunoblotting with HRP-conjugated anti-HA monoclonal antibodies (1:2,000 ...
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bioRxiv - Immunology 2023Quote: ... Equal amounts of protein were loaded and separated by electrophoresis on 12% SDS-PAGE gels and subsequently transferred to polyvinylidene fluoride (PVDF; Merck-Millipore, Germany). After blocking with 10% skim milk for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS) polyacrylamide gels and blotted onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Darmstadt, Germany). After blocking for 1 h with 5% bovine serum albumin ...
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bioRxiv - Microbiology 2020Quote: ... and HA-tagged proteins of samples were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and were transferred to a PVDF membrane (Merck Millipore, Tokyo, Japan). The membrane was incubated with rabbit anti-N antiserum or mouse anti-GFP antibody ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Final concentration was measured by Qubit™ RNA BR Assay Kit (Thermo Fisher Scientific. RNA integrity was assessed by gel electrophoresis with 1:1 formamide (Merck Millipore) and 1 µl of loading dye on 1.5% agarose gel ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amount of lysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride membrane (Merck Millipore, Burlington, MA, USA) in 25 mmol/L Tris ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were separated through electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel and then transferred onto an Immobilon-P membrane (Merck Millipore, Burlington, MA, USA). After blocking with 5% bovine serum albumin ...
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bioRxiv - Pathology 2022Quote: ... 50 μg of protein was separated via SDS-polyacrylamide gel electrophoresis (SDS–PAGE; Bio–Rad Laboratories, Inc., Hercules, CA, USA) and transferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Burlington, MA, USA). Then ...
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bioRxiv - Systems Biology 2024Quote: Cells were analysed for protein expression by SDS polyacrylamide gel electrophoresis and Western blot analysis using the following antibodies: mouse monoclonal anti α-tubulin (#05-829, Merck Millipore, Burlington, US), rabbit α-p-AKTSer473 (#9271) ...
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bioRxiv - Immunology 2024Quote: ... Equal amounts of protein were then separated through 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore Ltd., Tullagreen, Ireland). Membranes were blocked in 5% nonfat dry milk in 1× TBST for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: Periodic acid-Schiff (PAS) (periodic acid, Merck-Millipore ...
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bioRxiv - Microbiology 2024Quote: ... Samples were separated on a 1.2 % agarose gel stained with 0.01 % (v/v) GelGreen Nucleid Acid Stain (Merck, Darmstadt, Germany) in TBE buffer for 2 h at 120 V ...
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bioRxiv - Neuroscience 2024Quote: ... Fatty acids used were palmitic acid (W283215, Merck), stearic acid (10002390 ...
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Notch signaling functions in non-canonical juxtacrine manner in platelets to amplify thrombogenicitybioRxiv - Cell Biology 2022Quote: ... CaCl2 and reagents for electrophoresis were purchased from Merck. All other reagents were of analytical grade ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... oleic acid and linoleic acid were obtained from Merck, all of them with a purity higher than 97%.
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bioRxiv - Genomics 2021Quote: ... and gentisic acid (2,5-dihydroxybenzoic acid; Merck, Product Number: 841745) was performed in AT minimal medium (86 ...
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bioRxiv - Microbiology 2020Quote: ... Formic acid (Merck) was added to end the reaction (5% v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Trichloroacetic acid(Merck), Fetal Bovine Serum (Merck ...
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bioRxiv - Immunology 2023Quote: ... with Ehrlich’s reagent (Sigma)/perchloric acid (Merck) and absorbance was measured at 557 nm ...