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Citations for Merck :
1 - 50 of 1065 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and nucleoproteins were extracted using a nuclear protein extraction kit (Merck Millipore, Darmstadt, Germany). Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS ...
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bioRxiv - Genomics 2022Quote: ... followed by quenching with final 1 x Glycine solution for 5 min at RT utilizing the Magna Nuclear RIP (Cross-Linked) Nuclear RNA-Binding Protein Immunoprecipitation Kit (Merck millipore, 17-10520). Cross-linked cells were then centrifuged at 800 x g at 4°C and washed three times with ice cold DPBS ...
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bioRxiv - Cancer Biology 2021Quote: Intracellular protein was extracted from cells using the NucBuster Protein Extraction Kit (Merck) and quantified using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: Millipore Compartment Protein Extraction Kit (Merck-Millipore, Cat. #2145) was used to deplete cytosolic ...
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bioRxiv - Synthetic Biology 2023Quote: ... resuspended in BugBuster Protein Extraction Reagent (Merck, Cat. No. 70584) as per vendor’s instruction and then incubated at room temperature for 15 min with rotation ...
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bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
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bioRxiv - Genetics 2020Quote: ... Protein extraction and Western blot using anti-puromycin antibody (Merck Millipore) was performed as described before.
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bioRxiv - Plant Biology 2023Quote: ... The resin was pelleted as above and washed twice with 0.8 mL plant extraction buffer (see plant protein extraction section above) supplemented with 10 μM MG132 (Merck). Cold plant extract was either added directly to the resin (Supplementary Dataset S1 ...
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bioRxiv - Microbiology 2020Quote: Infected insect cells were disrupted with CytoBuster™ protein extraction reagent (Merck) and clarified by centrifugation at 4,300 × g for 20m before column loading ...
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bioRxiv - Microbiology 2021Quote: ... and the bacterial pellets were resuspended in BugBuster protein extraction reagent (Merck, Germany). The cell suspensions were incubated on a rotary shaker for 20 minutes ...
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bioRxiv - Developmental Biology 2020Quote: hESCs were harvested for protein extraction by addition of CelLytic M lysis buffer (Merck), freshly supplemented with protease inhibitors (PIC ...
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bioRxiv - Microbiology 2023Quote: ... and pellets were diluted in 1× BugBuster® (10× Protein Extraction Reagent, Novagen® Merck), 50 mM NaH2PO4 pH 8 ...
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bioRxiv - Cancer Biology 2019Quote: ... mouse anti-human nuclear antigen (1:100; Merck), Alexa Fluor 647-conjugated rat anti-CD45 (1:100 ...
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bioRxiv - Biochemistry 2022Quote: ... Cell lysis and membrane protein extraction was performed using the ProteoExtract Native Membrane kit (Merck Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... Cells were pelleted by centrifugation and lysed in Bugbuster™ protein extraction reagent (Merck; Darmstadt, Germany) by continuous agitation for 20 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... the pellets were washed first with 1× BugBuster® (10× Protein Extraction Reagent Novagen® Merck), then twice with water and once with 50 mM NaH2PO4 (pH 8) ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclear lysates were treated with Benzonase (Merck-Millipore, 70664). Nuclear lysates were pre-cleared on 25 μL Dynabeads M-280 Sheep anti-mouse or antirabbit IgG (Thermo ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclear staining was performed with Hoechst (Merck, cat #94403) for 15 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and SmWRKY27-GST were purified using a NucBuster™ Protein Extraction Kit (Cat. #71183; Merck KGaA, Darmstadt, Germany) for use in further EMSA experiments ...
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bioRxiv - Bioengineering 2022Quote: ... Cell nuclei were stained using 0,1% nuclear fast red (Merck). After finishing the staining procedure ...
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bioRxiv - Microbiology 2020Quote: ... Collected cells were immediately spun down and resuspended in BugBuster® Protein Extraction reagent (Novagen, Merck and Co. Inc) to lyse the bacterial cells and release proteins ...
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bioRxiv - Biochemistry 2020Quote: ... Portions of 10 g cell pellet were resuspended in 50 mL Bugbuster (BugBuster® Protein Extraction Reagent, Merck Millipore) supplemented with 5 µL Benzonase Nuclease (Novagen ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Microbiology 2021Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Plant Biology 2021Quote: ... The cultured cells were collected by centrifugation (8,000 × g for 10 min at 4 °C) and suspended in BugBuster Protein Extraction Reagent according to the manufacturer’s procedures (Merck Millipore). The supernatant was collected by centrifugation (12,000 × g for 10 min at 4 °C) ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were lysed in a protein extraction solution (∼10 µL/ mg tissue) consisting of 0.32M sucrose with protease inhibitors (Merck #4693159001) and phosphatase inhibitors (Pierce # A32957 ...
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bioRxiv - Cell Biology 2021Quote: RIP assays were performed using a Magna Nuclear RIP Kit from Merck Millipore (17-10520 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... after contrast staining with Kernechtrot (NUCLEAR FAST RED, C.I.60760, Merck-Millipore) for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... and the nuclear stain Hoechst 33258 (2 ug/mL, Sigma-Aldrich, Merck) for 2 h at RT ...
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bioRxiv - Physiology 2023Quote: ... For protein extraction the tissue was homogenised in RIPA buffer with a cocktail of phosphatase inhibitors (Sigma-Aldrich, Merck, Gillingham, Dorset), using a sonicator three times for 30s each time ...
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bioRxiv - Neuroscience 2022Quote: ... The pellets were lysed for 1 h using cell protein extraction reagent (CW BioTech, CW0889, China) with protease inhibitor Cocktail set III (1 %, v/v, Merck, 535140, Germany), followed by centrifugation at 500×g for 10 min and the supernatants were collected ...
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bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... followed by contrast staining with Kernechtrot (NUCLEAR FAST RED, C.I.60760, Merck-Millipore) for 5 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... which was performed using the EZ-Nuclear RIP Kit (17-701, Merck Millipore) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... at 1:500 dilution and nuclear stain DAPI (1 μg/ml, Merck, D9542) at 4°C with gentle rocking ...
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bioRxiv - Immunology 2022Quote: ... Tissue samples for RNA-protein extraction were cut into small pieces (< 5 mm) and placed in a microcentrifuge tube containing RNAlater® solution (Merck Ltd, Dorset, UK) and stored at −70°C until further use.
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bioRxiv - Developmental Biology 2021Quote: ... embryos were washed with PBS-Tween20 (0.1%) and a nuclear counterstain with DAPI (Merck, 1246530100) 1:1000 was done.
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bioRxiv - Developmental Biology 2022Quote: ... we performed nuclear hashtagging of two separate aggregates using single nuclei hashing antibodies (MAb414, Merck). To this end ...
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bioRxiv - Biochemistry 2022Quote: ... The two aqueous streams were supplied with the cell solution and with a 3 μM substrate solution containing lysis agents (0.7× BugBuster protein extraction reagent, Merck Millipore; 60 kU/mL rLysozyme, Novagen) in droplet assay buffer ...
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bioRxiv - Neuroscience 2021Quote: ... covered with mounting medium conjugated with a nuclear cell body marker (Fluoroshield TM with DAPI, Merck) and protected using standard coverglass ...
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bioRxiv - Molecular Biology 2023Quote: ... Nuclear translocation of FLAG-ER-SAMD1 was induced by adding 200 nM 4-OHT (Merck; 68392-35-8) for 24 h.
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bioRxiv - Cancer Biology 2021Quote: ... and lysed using PhosphoSafeM Extraction Reagent (Merck-Millipore, Watford, UK), according to manufacturer’s protocols ...
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bioRxiv - Biochemistry 2023Quote: ... RNA extraction was performed by adding TRI Reagent (Merck, T9424) to the fractions at a 1:1 v/v ratio ...
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bioRxiv - Cell Biology 2021Quote: ... the nuclear pellet was resuspended in 0.5 mL of ice-cold RIPA buffer supplied with 1 U/mL Benzonase (Merck), and incubated on a rotator for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mM K4Fe(CN)6 and 1 mg/ml X-gal (Roth)) and counterstained with nuclear fast red (Merck) for 5 min ...
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bioRxiv - Neuroscience 2021Quote: Cells for RNA extraction were lysed in TRI Reagent (T3809, Merck) for 5 minutes at room temperature and RNA was extracted from TRI Reagent according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Live cell fluorescence imaging was performed as previously described 78 with the minor modification of nuclear staining with Hoechst (Merck) instead of DAPI at a final concentration of 5 μg/ml ...