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Citations for Merck :
1 - 50 of 327 citations for Nipah virus Nucleoprotein N His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... Primary his tag antibody (05-949, Merck), stored in a skim milk solution ...
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Immunology 2022Quote: ... The N-terminal tag was removed by an overnight incubation with thrombin (Merck). Undigested protein was removed by IMAC ...
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bioRxiv - Plant Biology 2022Quote: ... The filtered supernatant was loaded onto a column packed with cOmpleteTM His-tag purification resin (Merck). His6-proteins were eluted with 4x 2 bead volumes of elution buffer (20 mM Na HEPES ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... and applied directly onto a column of 2 ml pre-equilibrated Ni-NTA beads (cOmplete His-Tag Purification Resin from Merck). After several washes with high salt (500 mM NaCl) ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was loaded on to a gravity flow-based Ni-NTA metal affinity column (2 ml beads, cOmplete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... WT—or its mutants fused with a His-tag on the amino-terminus—were expressed in Escherichia coli BL21 (DE3) using pET15b vector (Merck). A single colony was inoculated in 2 mL Luria-Bertani broth (LB ...
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a gravity flow-based Ni-NTA metal affinity column (2 ml beads, complete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged Trx1 was purified on a Ni2+ sepharose resin following the manufactureŕs instructions (cOmplete™ His-Tag Purification Resin, Merck).
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bioRxiv - Microbiology 2021Quote: ... The binding of CoVHH1-FLAG to S1 subunits-His tag was detected by incubating for 1 hour at room temperature with mouse monoclonal ANTI-FLAG® M2-Peroxidase (Merck) diluted with PBST ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tag-free Mpro in the flow-through was concentrated by using Amicon Ultra 15 centrifugal filters (10 kD, Merck Millipore) at 2773 x g ...
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bioRxiv - Microbiology 2023Quote: ... The mouse mAb that detects the IAV nucleoprotein was purchased from Merck (MAB8257). Secondary antibodies conjugated to AF405 and AF488 were purchased from Molecular Probes.
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bioRxiv - Immunology 2023Quote: ... Lysate was clarified by spinning cells for 10 min at 20,000 × g before loading supernatant onto cOmplete™ His-Tag purification resin (Merck; cat # 5893682001). Bound proteins were washed with 20 mM tris (pH 8.0) ...
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bioRxiv - Developmental Biology 2024Quote: ... and 0.1% N,N,N’,N’-Tetramethylethylenediamine (Merck), to make the gel at a stiffness of 100KPa ...
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bioRxiv - Cell Biology 2020Quote: ... and nucleoproteins were extracted using a nuclear protein extraction kit (Merck Millipore, Darmstadt, Germany). Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS ...
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bioRxiv - Biochemistry 2021Quote: ... were constructed using standard genetic engineering techniques with a His6 tag at the N-terminus using the pET28a vector (Novagen, Merck). The recombinant CAHS1 protein ...
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bioRxiv - Biochemistry 2021Quote: ... varieornatus CAHS1 protein was constructed and cloned as a fusion protein with a hexahistidine (His6) tag at the N-terminus using a pET28a vector (Novagen, Merck). CAHS1-N (Met1-Ser96 ...
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bioRxiv - Plant Biology 2023Quote: ... the sequence of CBL1 was fused with an N-terminal 2xStrepII tag in the pET-24b vector (Merck, Darmstadt, Germany). Sequences of active CPK5 and CIPK26 were fused with an N-terminal 2xStrepII tag in the pIVEX-1.3WG vector (Biotechrabbit ...
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bioRxiv - Microbiology 2023Quote: N,N,N’,N’-tetrakis-(2-pyridylmethyl)ethylenediamine (TPEN) (Merck, 616394), made up in DMSO and used at 5 µM ...
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bioRxiv - Biochemistry 2023Quote: The SUN domain sequence of human SUN1 (amino-acids 616-812) was fused to a TEV-cleavable N-terminal His6-GCN4 tag and cloned into a pRSF-Duet1 vector (Merck Millipore). The KASH domain sequence of human JAW1/LRMP (amino-acids 515-555 and 531-555 for KASH6 and KASH6 core ...
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bioRxiv - Cell Biology 2023Quote: ... FLAG tag (Merck, Darmstadt ...
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bioRxiv - Cell Biology 2022Quote: ... It was inserted with N and C-terminal tags (His6 and StrepII, respectively) in the baculovirus transfer vector pIEX/Bac3 (Merck Novagen #717263).
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bioRxiv - Microbiology 2022Quote: N,N-dimethylethylenediamine (Merck, 95%), 4-chloro-7-nitrobenzofurazan (NBD-Cl ...
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bioRxiv - Molecular Biology 2022Quote: ... or anti-His (Merck) for His-G5845–1508) ...
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bioRxiv - Bioengineering 2022Quote: ... 4 μl of N,N,N’,N’-Tetramethylethylendiamine (TEMED, #612-103-00-3, Sigma-Aldrich now Merck) was added to 1 ml of the monomer solution ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Immunology 2021Quote: ... dissolved in N,N-dimethylformamide (Merck Millipore) as substrate ...
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bioRxiv - Microbiology 2024Quote: ... anti-HA tag (Merck, H9658), anti-strep tag (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... was dissolved in N,N-dimethylformamide (Merck Millipore) and used for peroxidase-dependent staining ...
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bioRxiv - Cell Biology 2022Quote: ... 0.15 mg/ml N,N’-Methylenebisacrylamide (Merck, M7279), 11.7 mg/ml NaCl in 1x PBS) ...
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bioRxiv - Biochemistry 2019Quote: ... Bio-Lab Ltd, Jerusalem, Israel) in N,N-dimethylformamide (DMF, Bio-Lab Ltd, Jerusalem, Israel) combined with N,N-Diisopropylethylamine (DIPEA, Merck, Rehovot, Israel) for a 1 h cycle ...
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bioRxiv - Plant Biology 2021Quote: ... α-His6 (anti-polyhistidine tag antibody, Merck), and α-MAR/PAR (anti-pan-ADP-ribose binding reagent ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Staining was performed on fixed cells using an antibody directed against the hCoV-OC43 nucleoprotein as a primary antibody (Merck #MAB9013) followed by a horse radish peroxidase labeled detection antibody (Thermo Scientific #31430 ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% HI-FBS (Merck/Biochrom), 2 mM L-glutamine (Merck/Biochrom) ...
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bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
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bioRxiv - Bioengineering 2022Quote: ... 1.335 g N,N-dimethylacrylamide (DMAA, #274135, Sigma-Aldrich now Merck) and 0.32 g sodium acrylate (SA ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit polyclonal anti-HA-tag (#H6908, Merck), rabbit polyclonal anti-RNF213 (#HPA003347 ...
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bioRxiv - Molecular Biology 2020Quote: ... the virus-containing supernatant (8ml) was filtered (Steriflip; Merck) before being concentrated 20x (14000 rpm ...
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bioRxiv - Developmental Biology 2023Quote: ... or N-Butyl phosphate (NBP, mixture of mono-N-Butyl phosphate and di-N-Butyl phosphate) of >95% purity (808555, Merck), were prepared by diluting in dimethyl sulfoxide (DMSO) ...
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bioRxiv - Cell Biology 2021Quote: ... N’-dimethylformamide (Merck: D4451) to make a 10mg/ml Naphthol AS-MX phosphate “Solution A” stock ...
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bioRxiv - Biochemistry 2020Quote: ... N-hexane p.a (Merck), ethyl acetate p.a (Merck) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1.25mM N-acetylcysteine (Merck), 10mM nicotinamide (Merck) ...
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bioRxiv - Bioengineering 2023Quote: ... N-Hydroxysuccinimide (804518, Merck); Chlorpromazine hydrochloride (sc-202537 ...
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bioRxiv - Cell Biology 2024Quote: ... N’-Methylenebisacrylamide (Merck, M1533), Sodium acrylate (Merck ...