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Citations for Merck :
1 - 50 of 75 citations for Lassa Fever Virus Nucleoprotein NP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 1% NP-40 (Merck), 1m DDT (Merck) ...
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bioRxiv - Microbiology 2023Quote: ... The mouse mAb that detects the IAV nucleoprotein was purchased from Merck (MAB8257). Secondary antibodies conjugated to AF405 and AF488 were purchased from Molecular Probes.
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bioRxiv - Cell Biology 2020Quote: ... and nucleoproteins were extracted using a nuclear protein extraction kit (Merck Millipore, Darmstadt, Germany). Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS ...
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bioRxiv - Neuroscience 2023Quote: ... 1% NP-40 substitute (Sigma-Aldrich, Merck, Munich ...
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bioRxiv - Cell Biology 2020Quote: ... Staining was performed on fixed cells using an antibody directed against the hCoV-OC43 nucleoprotein as a primary antibody (Merck #MAB9013) followed by a horse radish peroxidase labeled detection antibody (Thermo Scientific #31430 ...
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bioRxiv - Bioengineering 2019Quote: ... The NPs were washed three times in ethanol (99 % absolute, Merck) and three times in deionized water ...
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bioRxiv - Cell Biology 2022Quote: ... 0.5% NP-40 and Complete EDTA-free protease inhibitor (Merck, 539134)] ...
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bioRxiv - Cell Biology 2023Quote: ... 0.3 percent NP-40) with fresh Proteinase K (Merck Cat 03115887001) at 55 °C overnight followed by 15 min of inactivation of proteinase K inactivated at 95 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 0.05% NP-40) supplemented with a protease inhibitor cocktail (Merck, #4693116001), and incubated on ice for 20 min ...
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bioRxiv - Molecular Biology 2020Quote: ... the virus-containing supernatant (8ml) was filtered (Steriflip; Merck) before being concentrated 20x (14000 rpm ...
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bioRxiv - Plant Biology 2020Quote: ... 1% NP-40 and protease inhibitor cocktail (cOmplete™, EDTA-free; Merck), phosphatase inhibitor cocktail 2 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... 0.5% NP-40) containing 1 mM PMSF and protease inhibitors (Merck-Millipore). The lysates were centrifuged at 12,000 g for 10 min and precipitated with anti-Flag antibody ...
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bioRxiv - Cell Biology 2022Quote: ... 0.5% NP‐40) supplemented with cOmplete Mini EDTA‐free protease inhibitor (Merck) for 30 min at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... 1% NP-40 and protease inhibitor cocktail (cOmplete™, EDTA-free; Merck). For Arabidopsis seedlings ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% NP-40 and protease inhibitor cocktail (complete Mini, Merck, Sigma Aldrich). Cells were lysed for 10 min incubation on ice and lysates were cleared with a 15 min centrifugation at 12000 g and 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... a calibration curve was plotted with p-NP as standard (Merck, Darmstadt, Germany). Endo-xylanase activity was determined using beechwod xylan (1% w/v ...
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bioRxiv - Immunology 2023Quote: ... permeabilised for 15 min with 0.5% NP-40 (Merck Life Science, Dorset, UK) then blocked for 1 h in PBS containing 0.1% Tween (PBST ...
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bioRxiv - Systems Biology 2023Quote: ... The fixative consisted of 4% formaldehyde in a PBS + 0.5% NP-40 (Merck) solution ...
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bioRxiv - Cell Biology 2021Quote: ... The virus was concentrated in a 100KDa Amicon centrifugation unit (Merck) at 5000g for 15 mins to achieve a 10-fold concentration ...
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bioRxiv - Neuroscience 2021Quote: ... This concentrated virus solution was filtered (0.22 μm Millex, Merck Millipore) and stored at 4 °C until further use ...
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bioRxiv - Evolutionary Biology 2023Quote: ... virus samples were mixed with SYBR Green I stain (Merck, Germany) and Tris-EDTA buffer in a PCR plate ...
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bioRxiv - Immunology 2023Quote: ... NP-Osu (Biosearch, cat no: N-1010-100) was dissolved in dimethylformamide (DMF) (Merck) to a concentration of 10mg/ml while vortexing ...
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bioRxiv - Microbiology 2020Quote: ... virus-containing supernatants were filtered through a 0.45 μm membrane (Merck Millipore). The amount of virus in the supernatants was quantified by RT assay ...
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bioRxiv - Cell Biology 2022Quote: ... Virus amplification was performed in Sf9 TriEX cells (Novagen / Merck #71023-3) grown in TriEx media (Novagen / Merck #71022) ...
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bioRxiv - Genetics 2022Quote: ... Virus was concentrated using Amcion Ultra-15 30k centrifugal filter (Merck#C7715) and centrifugation for 30 min at 3,900rpm.
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bioRxiv - Genomics 2024Quote: ... virus-containing supernatant was filtered through a 0.45 μm filter (Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed in Lysis Buffer (PBS with 0.1% NP-40) with protease inhibitor (Merck), 1 mM Na3VO4 ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated using Amicon Ultra-4 centrifugal filter units (Merck-Millipore) and the solution was filtered with a 0.2 µm Acrodisc syringe filter (Sigma-Aldrich).
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bioRxiv - Physiology 2021Quote: ... The virus was further purified using Sepharose G100 SF resin (Merck, Darmstadt, Germany) in Econopac colums (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... Virus was concentrated in PBS using Amicon Ultra-15 Centrifugal Filter Units (Merck) and stored at 4°C.31,77,78 Viral titre was quantified by inverted terminal repeat probe quantitative polymerase chain reaction (PCR) ...
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bioRxiv - Biochemistry 2023Quote: ... Virus- containing supernatant P1 was sterile-filtered using a 0.22 μm filter (Merck). To amplify the virus 1.5 mL P1 was added to 250 mL of Sf21 shaking cell culture at a density of 0.4*106 cells/mL ...
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bioRxiv - Neuroscience 2021Quote: Neurospheres (NP) were prepared in suspension as follows: NSCs were split with Accutase (Merck Millipore, SCR005) and 3 × 106 cells (9 × 102 cells/NP ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μl AAV virus was mixed with 1 μl 20% mannitol (MERCK K93152782 111). The virus and mannitol mixture were injected into a pulled-glass pipette (Warner Instruments ...
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bioRxiv - Biophysics 2023Quote: ... concentrated virus particles were freshly thawed and diluted in phosphate-buffered saline (PBS, Merck, 806552 ...
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bioRxiv - Cell Biology 2023Quote: ... 1% NP-40) containing protease and phosphatase inhibitors (cOmplete™ and PhosTOP™, Roche/Merck, Dorset, UK), 1 mM sodium orthovanadate and 1 mM phenylmethylsulfonyl fluoride and incubated for 30 min on ice.
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bioRxiv - Microbiology 2021Quote: ... Virus-infected culture was mixed with an equal volume of 0.4% Gelzan™ CM (Merck) and spread onto pre-warmed 0.7% Gelzan™ CM/SCV plates ...
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bioRxiv - Microbiology 2020Quote: ... virus-containing medium from producing cells was treated with 15 U/ml DNase I (Merck) and 10 mM MgCl2 for 5 h at 37°C prior to ultracentrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was added to RPE1-hTERT cells together with 8µg/ml polybrene (Merck Life science). Cells were selected using 5 µg/ml puromycin (Santa Cruz ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing cell supernatants were concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore) and exchanged into TexMACS media (Miltenyi Biotec).
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bioRxiv - Microbiology 2023Quote: ... virus stocks were concentrated on a 100 KDa cellulose membrane (Amicon Ultra-15, Merck Millipore) and rinsed three times successively with PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... virus at the MOI of 50 was applied with 8 μg/ml Polybrene (Merck Millipore). Virus-containing medium was replaced with medium containing puromycin (3.5 μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... 150 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 1:100 diluted EDTA-free protease inhibitor cocktail [Merck]) for 45 min before clarification (21,000×g ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The virus supernatants were directly overlaid on H1299 cells in the presence of polybrene (Merck, Germany) at a final concentration of 4 μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... 10mL of virus was concentrated using centrifugation through Amicon® Ultra-15 Centrifugal Filter Units (Merck). Efficacy against steel was assessed at room temperature and 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended ∼2x pellet volume extraction buffer (30 mM HEPES-NaOH pH = 7.8, 100 mM NaCl, 0.5% NP-40, 20 mM iodoacetamide (Merck, 407710), cOmplete™ ...
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bioRxiv - Biochemistry 2021Quote: HCT116 cells were lysed in RIPA buffer (for Mbd3) or Lysis Buffer (PBS with 0.1% NP-40) (for Tcf4) with protease inhibitor cocktail (Merck), 1 mM Na3VO4 ...
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bioRxiv - Biochemistry 2023Quote: ... coli pellet in 7.5 mL of TBS- N (Tris buffered saline with 0.1% NP-40) and supplemented with 1 x protease inhibitor cocktail tablet (Merck). Cells were sonicated for four rounds of one minute on ...
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bioRxiv - Neuroscience 2021Quote: ... Dissected tissue was placed in ice cold lysis buffer (150 mM NaCl, 1% NP-40, 50 mM Tris-HCl pH 8, and cOmplete Mini Protease Inhibitor, Merck) and homogenized with a syringe and 20G needle ...
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bioRxiv - Cancer Biology 2019Quote: ... The virus was harvested 48 hours post transfection and filtered through a 0.45 μm PES filter (Merck-Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... Respiratory syncytial virus RSV-G was immunoblotted with commercially available goat polyclonal anti-RSV serum (#AB1128, Merck). Coxsackievirus VP1 was immunoblotted with commercially available mouse monoclonal anti-VP1 (3A8 ...