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Citations for Merck :
1 - 50 of 223 citations for Hantaan Virus Nucleoprotein E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: 10His-tagged MA gene was expressed in Escherichia coli (E. coli) strain BL21 (DE3) (Merck, Darmstadt, Germany). The 10His-tagged MA protein was initially purified with nickel-NTA affinity column chromatography followed by TEV cleavage ...
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bioRxiv - Microbiology 2021Quote: ... One mL of virus stock and 9 mL of MEM-E supplemented with 1% pyruvate (Merck KGaA, Darmstadt, Germany) were added to a conical tube with 16 × 106 SK-6 cells and shaken for 30 minutes at 104 rpm and 37°C ...
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bioRxiv - Microbiology 2023Quote: ... The mouse mAb that detects the IAV nucleoprotein was purchased from Merck (MAB8257). Secondary antibodies conjugated to AF405 and AF488 were purchased from Molecular Probes.
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bioRxiv - Cell Biology 2020Quote: ... and nucleoproteins were extracted using a nuclear protein extraction kit (Merck Millipore, Darmstadt, Germany). Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS ...
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bioRxiv - Biochemistry 2019Quote: EcSerRS (from E. coli strain B ER2560) and SaSerRS (from S. aureus seg50 (1150)) were cloned into pET52b(+) vector (Merck Millipore, Germany) using the NcoI and SacI restriction sites allowing for the production of protein with a thrombin cleavable C-terminal His10-tag ...
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bioRxiv - Cell Biology 2024Quote: ... coli (Merck). Cells were grown at 37 °C in lysogen broth medium supplemented with 50 μg .mL−1 kanamycin (and 34 μg.mL−1 of chloramphenicol for Rosetta ...
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bioRxiv - Biochemistry 2023Quote: ... Silica gel (E, Merck) was used for flash chromatography ...
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Actin binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin IIbioRxiv - Biophysics 2022Quote: ... coli (Merck Millipore), and purified basically in the same way as Rng2CHD ...
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bioRxiv - Biochemistry 2020Quote: ... high ethanol concentration (E. Merck), Xylol ...
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bioRxiv - Biochemistry 2020Quote: ... HRP-conjugated streptavidin (E. Merck), 3.3 ‘diaminobenzidine (DAB) ...
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bioRxiv - Cell Biology 2023Quote: ... anti-E-cadherin (#MABT26, Merck), anti-Paxillin (#GTX125891 ...
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bioRxiv - Microbiology 2020Quote: ... coli ROSETTA strain (Merck) using pE-SUMO as an expression plasmid (86 ...
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bioRxiv - Cell Biology 2021Quote: ... coli BL21 (Novagen/ Merck Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... coli Rosetta2 (DE3) (Merck); TALON Metal Affinity Resin (Clontech) ...
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bioRxiv - Biochemistry 2023Quote: ... coli Rosetta2 (DE3) (Merck) was transformed using the constructed plasmid and cultured at 37°C in LB medium containing 30 μg/ml kanamycin and 34 μg/ml chloramphenicol ...
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bioRxiv - Biochemistry 2024Quote: ... coli C41(DE3) (Merck) co-transformed with pET22-Wscytc and pEC86 (which harbors the ccm genes for production of the machinery for c-type heme biosynthesis and maturation (Arslan et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Staining was performed on fixed cells using an antibody directed against the hCoV-OC43 nucleoprotein as a primary antibody (Merck #MAB9013) followed by a horse radish peroxidase labeled detection antibody (Thermo Scientific #31430 ...
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... E-cadherin (1:300, Merck, #U3254), α-actinin (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... E-Cadherin (1:600, Merck U3254), Glucagon (1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli Rosetta 2 cells (Merck) by induction with 0.2 mM isopropyl β-d-1-thiogalactopyranoside for 18 h at 18 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... coli Rosetta 2 (DE3) (Merck) and purified using Glutathione Sepharose High Performance beads (GE Healthacare ...
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bioRxiv - Cell Biology 2019Quote: ... coli Rosetta2 pLysS (Merck 71403). Overnight cultures were diluted 1:50 in a final volume of 50 ml ...
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bioRxiv - Cell Biology 2019Quote: ... coli Rosetta2 pLysS (Merck 71403) were used ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21 (DE3) cells (Merck) and the mutant proteins were expressed and purified according to the protocol described by Oliver ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Coli BL21 (DE3) cells (Merck) were transformed with the pET28a(+)-scDH and pET28a(+)-ΔscDH and grown in LB medium with 35 μg/ml kanamycin at 37 °C and shaken until an OD600 of ∼ 0.6 ...
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bioRxiv - Molecular Biology 2022Quote: ... coli BL21(DE3) star (Merck). Luria Bertani (LB ...
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bioRxiv - Genetics 2022Quote: ... coli O111:B4 (#LPS25, Merck) or Pam2CSK4 (#tlrl-pm2s-1 ...
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bioRxiv - Immunology 2023Quote: ... coli serotype O55:B5 (Merck) for 24 to 48 hours to arrive at a mature BMDC culture or alternatively frozen to -80°C in 90% FCS (Biowest ...
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bioRxiv - Immunology 2023Quote: ... coli serotype O55:B5 (Merck) for 24 to 48 hours to induce B-cell blast formation.
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bioRxiv - Microbiology 2023Quote: ... coli expression vector (Merck-Novagen) using Gibson assembly ...
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bioRxiv - Molecular Biology 2020Quote: ... the virus-containing supernatant (8ml) was filtered (Steriflip; Merck) before being concentrated 20x (14000 rpm ...
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bioRxiv - Neuroscience 2021Quote: ... and coverslipped with Entellan (E. Merck, Switzerland).
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bioRxiv - Microbiology 2020Quote: ... treatment with 80 µM E-64d (Merck), 80 µM Z-VAD-fmk (Invivogen ...
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bioRxiv - Microbiology 2020Quote: ... coli Rosetta (DE3) competent cells (Merck). A 10 mL overnight culture was used to inoculate 4 L of LB medium + carbenicillin + chloramphenicol ...
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bioRxiv - Immunology 2022Quote: ... coli (Novagen, Merck KGaA, Darmstadt, Germany) for subsequent recombinant protein production following induction with 1 mM IPTG (Carl Roth ...
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bioRxiv - Microbiology 2021Quote: ... coli Rosetta (DE3) competent cells (Merck) by heat-shock transformation ...
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bioRxiv - Biochemistry 2021Quote: ... coli Rosetta pRARE (Merck, Darmstadt, Germany).
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bioRxiv - Microbiology 2022Quote: ... coli strain Rosetta™ DE3 (Merck) at 37 °C (for candidates C05 ...
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bioRxiv - Biophysics 2022Quote: ... coli cells (Merck KGaA, Darmstadt, Germany) for protein expression ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli BL21 (DE3) (Merck; Darmstadt, Germany) and C41(DE3 ...
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bioRxiv - Plant Biology 2020Quote: ... coli BLR(DE3)-pLysS cells (Merck) were grown overnight at 37°C in Terrific Broth (TB ...
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bioRxiv - Biochemistry 2021Quote: ... coli Rosetta 2 (DE3) (Merck Millipore) after double-transformation of pET-Duet-1 (α- and γ-subunits ...
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bioRxiv - Biochemistry 2021Quote: ... coli Rosetta (DE3) cells (Merck, Germany) to perform expression via autoinduction ...
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bioRxiv - Microbiology 2019Quote: ... coli ORIGAMI (DE3, Merck, Darmstadt, Germany) cells according to the manufactureŕs instructions ...
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bioRxiv - Microbiology 2021Quote: ... coli Rosetta pRARE (Merck, Darmstadt, Germany).
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bioRxiv - Plant Biology 2023Quote: ... coli cells from Merck (Cat# CMC0014). Cells were grown in Luria broth or labeled M9 medium with kanamycin (His6-SUMO-SALTY) ...
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bioRxiv - Molecular Biology 2023Quote: ... coli Rosetta 2 (DE3) cells (Merck), and cultured in Terrific Broth medium at 37 °C and induced with IPTG at a final concentration of 500 μM ...
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bioRxiv - Microbiology 2023Quote: ... coli Rosetta pLysS (Novagen/Merck, Germany), inoculated from overnight culture to a starting OD600 of 0.1 ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 (DE3) cells (Novagen, Merck, Darmstadt ...
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bioRxiv - Immunology 2024Quote: ... coli BL21 (DE3) strain (Novagen, Merck) by overnight culturing at 30 °C in an auto-induction medium ...