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Citations for Merck :
1 - 50 of 931 citations for Glutathione S transferase Class mu 26 kDa Isozyme GST Schistosoma Japonicum since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... as a translational fusion with glutathione-S-transferase (GST) and purified with GST-Bind resin (Merck), as previously described (Sullivan et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... The glutathione-S-transferases (GST) fusion proteins were expressed in Escherichia coli and purified with glutathione-conjugated agarose beads (Merck) by standard procedures ...
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bioRxiv - Cell Biology 2022Quote: ... The eluate was concentrated down to 1.5 mL using an Amicon Ultra Centrifuge Filter unit (30 kDa cutoff for GST-UtrCH-sfGFP and 10 kDa cutoff for GST-LifeAct-sfGFP; Merck, UFC903024 and UFC901024, respectively). The GST tag was removed by digestion with 8 units/mL PreScission Protease (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... Rgl2RA-Halo or GST- Rgl2RA/GST-BRAFRBD fusion proteins were concentrated using a concentrator (10 KDa MWCO, Merck, Amicon Ultra centrifugal filters ...
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bioRxiv - Biochemistry 2021Quote: ... Double-capped 26S proteasomes were concentrated in 100 kDa Amicon Ultra (Merck) centrifugal filter (45 min ...
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bioRxiv - Cell Biology 2022Quote: ... Equal loading of the GST-UBAN beads was confirmed using ponceau S (Merck KGaA) staining.
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bioRxiv - Neuroscience 2020Quote: ... terminal deoxynucleotidyl transferase (3333566001, Merck), and rhodamine-conjugated antibodies to digoxigenin (11207750910 ...
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bioRxiv - Plant Biology 2023Quote: ... a second GST-tag purification was performed and the peak fractions were pooled and concentrated (Amicon® Ultra MWCO 10 kDa, Merck).
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bioRxiv - Biochemistry 2023Quote: ... at 4 °C for 30 s and concentrated in a 100 kDa Amicon Ultra centrifugal filter (Merck Millipore), which was equilibrated with 1% GDN in IP buffer ...
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bioRxiv - Systems Biology 2021Quote: ... 30 kDa (idem, UFC903024), 50 kDa (idem, UFC905024), 100 kDa (idem, UFC910024) and 300 kDa (Merck, Vivaspin 20 centrifugal concentrator ...
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bioRxiv - Biophysics 2019Quote: ... For experiments involving glutathione (Merck) a 10 mM glutathione stock was prepared and added in a 1:1 (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... GST-agarose magnetic beads (Merck) were incubated with equimolar concentration of flag-tagged human RNase H1 and GST-CTD proteins in PBS buffer (pH ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit anti-GST (Merck,#ZRB1223,1:1000), mouse anti-β-actin (Santa Cruz Biotechnology,#SC-47778,1:1000) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted at 460 g at 4 °C for 30 s and concentrated in a 1% GDN pre-equilibrated 30 kDa Amicon Ultra centrifugal filter (Merck Millipore). The concentrated eluate was spun through a Zeba Spin Desalting Column 40 kDa MWCO (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... GST was labeled with mouse Anti-GST Tag monoclonal antibody (05-782, Merck Chemicals; 2.5 μg/ml). To account for variations in the amount of fusion protein ...
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bioRxiv - Developmental Biology 2020Quote: ... 100µM freshly purchased reduced glutathione (Merck Millipore) and 500µM H2O2 (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... MHC class II molecules were purified from supernatants with Chelating Sepharose FF (Merck). Peptide loading was performed in citrate saline buffer (100 mM citrate ...
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bioRxiv - Cell Biology 2020Quote: ... 100 kDa (Merck). The following antibodies used in this study were raised in mouse unless otherwise stated ...
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bioRxiv - Plant Biology 2021Quote: ... 10 kDa (Merck), desalted with PD 10 desalting columns (Merck ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated MHC class II-peptide complexes were purified on a HisTrap HP column (Merck) and kept at -80°C until multimerization with streptavidin conjugates.
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bioRxiv - Molecular Biology 2023Quote: ... the beads were resuspended in TE buffer with 40 mU/µL proteinase K (#P4850, Merck) and incubated for 2 h at 55 °C and 1200 rpm ...
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bioRxiv - Plant Biology 2021Quote: ... and detected with anti-GST monoclonal antibody (Merck), anti-GFP-HFP monoclonal antibody (Miltenyi Biotech) ...
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bioRxiv - Immunology 2020Quote: ... 350 ng/mL active ERK2-GST tagged (Merck) or 175 ng/mL ERK1 plus 175 ng/mL ERK2-GST for 30 min at 30°C.
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bioRxiv - Cancer Biology 2023Quote: ... and the membranes stained with 1) PKH-26 (Merck) and 2 ...
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bioRxiv - Immunology 2023Quote: ... The tetramer (428 kDa) was purified from excess GFP-biotin (25 kDa) with a 100 kDa cut-off Amicon Ultra centrifugal filter (Merck). Functionalised agarose beads were washed twice and then incubated with 20 nM of the GFP-streptavidin tetramers and anti-mouse IgG2a/b BV421 antibody (1:10 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... carbonic anhydrase (30 kDa) and cytochrome C (12 kDa; all from Merck). The relationship between elution volume and molecular mass determined using linear regression ...
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bioRxiv - Microbiology 2019Quote: ... 43.6 mM sodium phosphate) or 0.2 mU α-amylase (1,4-α-glucanase) from Bacillus licheniformis (Merck) in citrate-phosphate buffer at pH 7 (24.3 mM citric acid ...
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bioRxiv - Molecular Biology 2023Quote: ... the beads were resuspended in wash buffer supplemented with 20 mU/µL proteinase K (#P4850, Merck) and incubated with shaking for 20 min at 37□ and 300 rpm ...
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bioRxiv - Bioengineering 2022Quote: ... 10 kDa (Merck Millipore) in storage buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... (Merck, 100 KDa cutoff). Approximately 350 ml of culture fluid was concentrated 100-fold before RNA extraction.
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bioRxiv - Biochemistry 2022Quote: ... MWCO 3.5 kDa (Merck) at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... MWCO 50 kDa (Merck).
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bioRxiv - Biochemistry 2022Quote: ... MWCO 3.5 kDa (Merck) at 4 °C.
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bioRxiv - Immunology 2022Quote: ... buffer-exchanged on a HiPrep 26/10 desalting column (Merck) into AviTag buffer and subsequently biotinylated with the BirA enzyme according to the manufacturer instructions (Avidity ...
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bioRxiv - Cell Biology 2019Quote: ... GST-Mm-RAB8A-Q67L and GST-Rn-RAB27A-Q78L proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore, Novagen, Darmstadt, Germany). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Biochemistry 2019Quote: ... Microcon (30 kDa, Merck Millipore) and one sample was processed without prior filtering ...
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bioRxiv - Bioengineering 2020Quote: ... MWCO= 50 kDa (Merck Millipore) for KJ12A and KJ12B or MWCO = 30 kDa (Merck Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... 300 kDa MWCO cassette (Merck) before loading onto a pre-equlibrated POROS CaptureSelect AAVX affinity column (Thermo Fisher ...
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bioRxiv - Biochemistry 2022Quote: ... 100 kDa MWCO concentrator (Merck) before storage at −80°C.
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bioRxiv - Plant Biology 2024Quote: ... 3 kDa MWCO (Merck - UFC500324) and 0.5 µl were injected to LC-MS system ...
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bioRxiv - Molecular Biology 2024Quote: ... 300 kDa MWCO cassette (Merck) before loading onto a pre-equilibrated POROS CaptureSelect AAVX affinity column (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 kDa MWCO concentrator (Merck) before storage at −80 °C.
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bioRxiv - Molecular Biology 2021Quote: ... or already HRP-conjugated antibody (anti-GST-HRP, Merck RPN1236) was added for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The clarified supernatant was incubated with GST-agarose beads (Merck) overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell death was assessed by Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL; S7100, Merck Millipore). Images were obtained with a Leica DM3000 microscope with a mounted Leica DFC420 camera ...
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bioRxiv - Microbiology 2019Quote: ... smegmatis MC2 155 were prepared as described above and mixed with 0.2 mU of commercial cellulase (1,4-β-glucanase) from Trichoderma reesii ATCC 26921 (Merck) in 50 mM citrate-phosphate buffer at pH 5 (6.5 mM citric acid ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatin was enzymatically sheared for 5 min at 37°C with 0.2 mU/μL Micrococcal nuclease (MNase) (#N3755, Merck), and stopped with MNase stop buffer (110 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... The input control was incubated in TE buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA) supplemented with 40 mU/µL proteinase K (#P4850, Merck) for 2 h at 55°C and 1200 rpm ...
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bioRxiv - Bioengineering 2021Quote: ... or 40 kDa (FD40; Merck KGaA) were perfused through the chip at a flow rate of 20 µL/h for 60 min ...