Labshake search
Citations for Merck :
1 - 50 of 321 citations for ER beta Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2021Quote: ... Human pancreatic beta cells 1.4E7 were purchased from Merck and cultured in RPMI-1640 medium (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2021Quote: ... Human pancreatic beta cell line 1.4E7 was purchased from Merck and cultured in RPMI-1640 medium (Thermo Fisher Scientific ...
-
Integrated requirement of non-specific and sequence-specific DNA binding in MYC-driven transcriptionbioRxiv - Molecular Biology 2020Quote: ... ER-alpha (Merck, #06-935). Immunoprecipitated DNA was eluted in TE-2% SDS and crosslinks were reversed by overnight incubation at 65 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... R&D, AF2408), Goat-Anti Human SOX17 (1:100, R&D, AF1924) or Mouse Anti-Human Beta-Tubulin (1:1000, Merck, T8660) primary Chicken Anti-Human MAP2 (1:2000 ...
-
bioRxiv - Genomics 2020Quote: ... was incubated with/without 0.4μg human 6X-His-CDK9/Cyclin T1 (Merck, 140685) and/or 0.4μg human GST-tagged PP2A-α (Sigma ...
-
bioRxiv - Developmental Biology 2022Quote: ... and beta-mercaptoethanol (Merck, M6250) according to manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2020Quote: ... using a Millicell ERS-2 Electrical Resistance System (Merck-Millipore). To calculate transendothelial electrical resistance (TEER ...
-
bioRxiv - Cell Biology 2021Quote: ... TEER was measured with the Millicell ERS-2 Voltohmmeter (Merck Millipore). TEER values (Ω cm2 ...
-
bioRxiv - Bioengineering 2023Quote: ... R was measured using Millicell ERS-2 Volt/Ohmmeter (Merck, MERS00002). All procedures were performed on a 38 °C hotplate ...
-
bioRxiv - Genomics 2020Quote: ... and Tubulin beta 4 (#T7941, Merck). To do so ...
-
bioRxiv - Molecular Biology 2022Quote: ... or anti-His (Merck) for His-G5845–1508) ...
-
bioRxiv - Neuroscience 2022Quote: ... and 25 nM beta-mercaptoethanol (8057400005, Merck). The embedded EBs were cultured in the stationary condition in 6-well plates for 4 days ...
-
bioRxiv - Neuroscience 2023Quote: ... An anti-beta-actin (Merck Group, A1978) antibody was used as loading marker ...
-
bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
-
bioRxiv - Microbiology 2023Quote: ... mouse anti-beta-tubulin MAb (Merck: 05-661)) in 5% skim milk in T-PBS buffer at room temperature for 1 hr ...
-
bioRxiv - Neuroscience 2022Quote: ... were used to assess the difference in beta-secretase activity in each anatomical region using the Beta-Secretase Activity Fluorometric Assay Kit (Merck, MAK237).
-
bioRxiv - Immunology 2019Quote: We measured ALI culture TEER with a Millicell ERS-2 Voltohmmeter (Merck Millipore, USA). The final unit area resistance (Ω*cm2 ...
-
bioRxiv - Microbiology 2020Quote: ... Infectivity has been tested by Beta-galactosidase assay (Merck) activity measured 48 h post infection according to manufacturer’s instructions ...
-
bioRxiv - Genomics 2023Quote: ... 1% of 55 mM beta-Mercaptoethanol solution (Merck Millipore). Upon thawing ...
-
bioRxiv - Microbiology 2023Quote: ... transepithelial electric resistance (TEER) measurements were performed using a Millicell ERS-2 Voltohmmeter (Merck-Millipore) equipped with an Ag/AgCl electrode (STX01 ...
-
bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Tubulin beta (1:8,000; clone KMX-1 from Merck Millipore), dMi-2 (1:8,000 ...
-
bioRxiv - Cell Biology 2019Quote: ... and immunollabeled with anti-beta III tubulin (Tubbeta3) (Merck Millipore) followed by incubation with fluorophore-conjugated secondary antibody (Alexa 488 or 594 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Mouse anti-beta-actin 1:10,000 (A5441, Merck, Darmstadt, Germany) was used as loading control ...
-
bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% HI-FBS (Merck/Biochrom), 2 mM L-glutamine (Merck/Biochrom) ...
-
bioRxiv - Systems Biology 2023Quote: ... Primary his tag antibody (05-949, Merck), stored in a skim milk solution ...
-
bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
-
bioRxiv - Biophysics 2021Quote: ... ER-Hoxb8 cells were cultured in uncoated six-well culture plates in RPMI 1640 medium (Merck Millipore) supplemented with 10% FBS (Pan Biotech) ...
-
bioRxiv - Cancer Biology 2021Quote: ... including 1% beta-mercaptoethanol (#M7522, Sigma, part of Merck, Darmstadt, Germany), snap frozen on dry ice and stored at −80°C ...
-
bioRxiv - Microbiology 2020Quote: ... TEER readings were measured using the electrode supplied along with the MilliCell-ERS volt-ohm meter (Merck, USA). A blank insert with media alone was used as a negative control ...
-
bioRxiv - Microbiology 2023Quote: Transepithelial electrical resistance (TEER) of the filter-grown monolayers was measured using a Millicell-ERS device (Merck Millipore) as an index for integrity of the tight junctions (TJ).
-
bioRxiv - Molecular Biology 2023Quote: ... Nuclear translocation of FLAG-ER-SAMD1 was induced by adding 200 nM 4-OHT (Merck; 68392-35-8) for 24 h.
-
bioRxiv - Molecular Biology 2024Quote: ... 1 × 107 wild-type U2OS or AsiSI-ER U2OS cells were crosslinked with 1% formaldehyde (Merck, 252549-100ML) for 10 min at 37°C and inactivated by the addition of glycine (Merck ...
-
bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
-
bioRxiv - Biochemistry 2024Quote: ... we utilized the His-Bind purification kit (Merck, USA) followed by manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2020Quote: The barrier integrity of the in vitro BBB models was assessed through measurements of TEER using Millicell ERS-2 epithelial volt-ohm Meter and STX01 Chopstick Electrodes (Merck Millipore). The TEER value for each hanging culture insert was obtained from an average of three individual measurements subtracted the TEER value of a double-coated cell-free hanging culture insert and multiplied by the area of the hanging culture insert (1.12cm2) ...
-
bioRxiv - Microbiology 2020Quote: ... used for barrier dysfunction experiments were conducted on a 37 °C heating block using a Millicell ERS-2 Voltohmmeter (Merck-Millipore) equipped with an Ag/AgCl electrode (STX01) ...
-
bioRxiv - Molecular Biology 2021Quote: TEER measurements [22] were performed on 3rd day of incubation with growth factors and (or) different inhibitors by using Millicell ERS-2 Electrical Resistance System (Merck-Millipore). Each insert was measured in three different locations ...
-
bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
-
bioRxiv - Biochemistry 2021Quote: ... The supernatant was added to a His Trap HP column (Merck) previously equilibrated with buffer A ...
-
bioRxiv - Cell Biology 2021Quote: ... previously coupled to 1 ml HI-TRAP NHS columns (GE/Merck) packed with Sepharose pre-activated for covalent coupling of ligands containing primary amino groups ...
-
bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Merck). Immortalized human C20 microglia were kindly provided by David Alvarez-Carbonell (21) ...
-
bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were resuspended in 1% beta-Mercaptoethanol (#M3148-100ml, Sigma, part of Merck, Darmstadt, Germany)-supplemented RLT lysis buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... Mouse monoclonal anti vinculin (V9131) and rabbit polyclonal anti beta actin (A2066) were from Merck. Rabbit polyclonal antibodies to ARL8b (A88749) ...
-
bioRxiv - Neuroscience 2021Quote: TEER across cellular monolayers was measured with chopstick electrodes in 24-well Transwell inserts using the Millicell® ERS-2 Voltohmmeter (Merck Millipore). The absolute TEER of eGFP-hCldn5-MDCK II cells before treatment was recorded as a baseline reading ...
-
bioRxiv - Microbiology 2021Quote: ... and the supernatant was incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
-
bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a His-bind resin column (Merck Millipore) (1 ml ...
-
bioRxiv - Plant Biology 2023Quote: ... and purified with Ni-NTA His•Bind® Resin agarose (Merck, 70666). The binding reaction was performed in 20 µL of binding buffer [10 mM Tris (pH 8.0) ...
-
bioRxiv - Biochemistry 2023Quote: ... His tagged proteins were labeled using Ni-NTA-ATTO 488 (Merck Group) at a ratio of ATTO/protein = 1/500 in MN buffer (50 mM MES pH 6 ...