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Citations for Merck :
1 - 50 of 3185 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Infected insect cells were disrupted with CytoBuster™ protein extraction reagent (Merck) and clarified by centrifugation at 4,300 × g for 20m before column loading ...
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bioRxiv - Cell Biology 2022Quote: ... Virus amplification was performed in Sf9 TriEX cells (Novagen / Merck #71023-3) grown in TriEx media (Novagen / Merck #71022) ...
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bioRxiv - Molecular Biology 2019Quote: ... We obtained insect Sf9 cells from Merck and cultivated them in the Sf900 medium (Gibco ...
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bioRxiv - Immunology 2023Quote: ... coli serotype O55:B5 (Merck) for 24 to 48 hours to arrive at a mature BMDC culture or alternatively frozen to -80°C in 90% FCS (Biowest ...
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bioRxiv - Immunology 2023Quote: ... coli serotype O55:B5 (Merck) for 24 to 48 hours to induce B-cell blast formation.
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bioRxiv - Neuroscience 2020Quote: ... serotype O111:B4 were purchased from Merck Millipore Ltd ...
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bioRxiv - Immunology 2023Quote: ... coli serotype 0111:B4 (Sigma-Aldrich/Merck) was used as a control of macrophage stimulation ...
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bioRxiv - Microbiology 2021Quote: ... the pUL21 gene was amplified from virus stock by PCR with the oligonucleotide primers 5′-ATGGAGCTTAGCTACGCCAC-3′ and 5′-TTTATTGGGGTCTTTTACACAGACTGTC-3′ using KOD polymerase (Merck) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was estimated using BCA protein assay kit (Merck, 71285-3, Germany).
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bioRxiv - Neuroscience 2020Quote: ... a short protein sequence expressed in the injected virus (1:400 rabbit anti P2A; ABS31, Merck, Darmstadt, Germany). In the third step ...
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Microbiology 2020Quote: ... virus-containing medium from producing cells was treated with 15 U/ml DNase I (Merck) and 10 mM MgCl2 for 5 h at 37°C prior to ultracentrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was added to RPE1-hTERT cells together with 8µg/ml polybrene (Merck Life science). Cells were selected using 5 µg/ml puromycin (Santa Cruz ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing cell supernatants were concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore) and exchanged into TexMACS media (Miltenyi Biotec).
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bioRxiv - Developmental Biology 2022Quote: Ovaries were dissected in Shields and Sang M3 Insect Medium (S3652, Merck). Ovaries were treated for 1 hour at RT with 100 μM BMH-21 or DMSO control ...
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bioRxiv - Developmental Biology 2022Quote: ... ovaries were dissected in Shields and Sang M3 Insect Medium (S3652, Merck) and were immediately transferred after dissection to fresh medium containing a 1:400 dilution of Click-iT OPP Reagent (OP-puro 50 μM) ...
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bioRxiv - Biochemistry 2023Quote: ... with the baculoviral DNA using the Insect GeneJuice transfection reagent (Merck Milipore). The virus was amplified by infecting Sf9 cells in the presence of 2 % fetal bovine serum and incubating for 72h at 27 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The virus supernatants were directly overlaid on H1299 cells in the presence of polybrene (Merck, Germany) at a final concentration of 4 μg/ml ...
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bioRxiv - Bioengineering 2023Quote: ... Wells were then blocked with protein block (3% BSA fraction V (Merck) or 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Cells were fixed with 3% paraformaldehyde (Merck) and stained with anti-perforin Ab and phalloidin-AF 488 ...
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bioRxiv - Developmental Biology 2023Quote: ... was diluted to a final concentration of 20 μm in Grace’s insect medium (Merck). Pupae were injected at 50% pupal development using a Hamilton microliter syringe (701N) ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were concentrated using a centrifugal filter unit (3 kDa MWCO, Merck Millipore) at 3500 rpm and 4°C ...
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bioRxiv - Microbiology 2020Quote: ... virus inoculum was removed from each well and cells were overlaid with 300 μL of 0.6% carboxymethylcellulose (Merck) diluted in DMEM supplemented with 2% FBSi ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were transduced with 1 MOI of concentrated virus containing 8 ug/mL Polybrene (Merck, TR-1003-G). Two days later ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained with 1 µg (1:100 dilution) of a anti-Rabies Virus CVS-11 antibody (Merck) or anti-VZV gE mouse antibody (Abcam ...
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bioRxiv - Molecular Biology 2020Quote: ... the virus-containing supernatant (8ml) was filtered (Steriflip; Merck) before being concentrated 20x (14000 rpm ...
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bioRxiv - Immunology 2021Quote: ... Cells were fixed with 3% glutaraldehyde (Merck, 1042390250) in 0.1 M mNa-phosphate buffer (pH 7.4) ...
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bioRxiv - Biochemistry 2021Quote: ... and the protein was concentrated using Amicon 3 kDa MWCO centrifugal filters (Merck Millipore). The protein concentration was calculated using the measured absorbance at 280 nm under native conditions and assuming an extinction coefficient of 19940 M-1 cm-1.
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bioRxiv - Bioengineering 2019Quote: ... Eluted proteins were dialyzed using a 3 kDa membrane D-Tube Dialyzer (Merck, Germany) to remove the imidazole in Storage Buffer (200 mM ammonium acetate ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was concentrated with an Amicon Ultra-4 (Merck Millipore, MWCO 3 K) to a concentration of 726 µM ...
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bioRxiv - Cell Biology 2022Quote: ... 293T or 293GP virus producing cells were transfected using the GeneJuice reagent according to the manufacturer’s recommendations (Merck Millipore). Two days after transfection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... One milliliter of the virus supernatants was directly overlaid on the cells in the presence of polybrene (Merck, Germany) at a final concentration of 4 μg/ml ...
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bioRxiv - Microbiology 2020Quote: ... 96-well plates with confluent cell layers were fixed and virus was inactivated by immersion in 100% methanol (MERCK) for 20 min ...
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bioRxiv - Genomics 2023Quote: ... The transduction was performed by adding the virus particle directly onto the cells supplemented with 6μg/mL polybrene (Merck). The cells were refreshed 24h after transduction and the antibiotics (puromycin/blasticidin ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were concentrated using 3 kDa MWCO Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore). Protein concentrations were determined using the Proteins and Labels function on a Thermo Scientific™ NanoDrop™ One Microvolume UV-Vis Spectrophotometer and subsequently confirmed on SDS-PAGE gel upon Coomassie staining and colorimetric imaging on an Amersham ImageQuant 800 CCD Imager.
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bioRxiv - Biophysics 2023Quote: ... The final purified protein was concentrated to 1 mM using Amicon (3 kDa cutoff, Merck) and exchanged with NMR buffer D (10 mM sodium phosphate ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
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bioRxiv - Cancer Biology 2021Quote: Intracellular protein was extracted from cells using the NucBuster Protein Extraction Kit (Merck) and quantified using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... The two aqueous streams were supplied with the cell solution and with a 3 μM substrate solution containing lysis agents (0.7× BugBuster protein extraction reagent, Merck Millipore; 60 kU/mL rLysozyme, Novagen) in droplet assay buffer ...
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bioRxiv - Immunology 2022Quote: ... and were maintained in Schneider’s Insect Medium with L-glutamine and sodium bicarbonate (Merck KGaA, Darmstadt, Germany) supplemented with 10 % (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... The virus was concentrated in a 100KDa Amicon centrifugation unit (Merck) at 5000g for 15 mins to achieve a 10-fold concentration ...
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bioRxiv - Neuroscience 2021Quote: ... This concentrated virus solution was filtered (0.22 μm Millex, Merck Millipore) and stored at 4 °C until further use ...
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bioRxiv - Evolutionary Biology 2023Quote: ... virus samples were mixed with SYBR Green I stain (Merck, Germany) and Tris-EDTA buffer in a PCR plate ...
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bioRxiv - Cell Biology 2021Quote: ... Purified proteins were were concentrated > 60 μM using Amicon Ultra centrifugal filters (Merck; MWCO 3 kDa), rebuffered to 50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were concentrated using a centrifugal filter unit (3 kDa MWCO, Merck Millipore; Cat. No. UFC900324) at 3500 rpm and 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... and the protein was concentrated in a centrifugal concentrator (Amicon MWCO 3 kDa: Merck, Darmstadt, Germany) and stored at −20 °C in dialysis buffer (150 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... The pure protein obtained was concentrated using a 3 kDa Amicon® Ultra Centrifugal Filter (Merck).
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bioRxiv - Molecular Biology 2023Quote: ... Protein mixtures were incubated with 40μl PBST washed Streptavidin agarose resin (50% slurry, 69203-3 Merck) and incubated for 2h at room temperature on a roller ...
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bioRxiv - Microbiology 2020Quote: ... cells were treated with MG132 overnight (ON, 3 μM, Merck). Cells were lysed in ice-cold RIPA buffer (150mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then fixed with 3% glutaraldehyde (Merck, Germany) in sodium phosphate buffer (0.1 M ...