Labshake search
Citations for Merck :
2801 - 2850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... Chromatin immunoprecipitation was performed using 5-10 μg of primary mouse monoclonal ANTI-FLAG® M2 antibody (Merck, F1804-200UG) in 250 μL LB3 with 1% Triton X-100 overnight at 4 °C ...
-
bioRxiv - Molecular Biology 2024Quote: Candidate genes were amplified from Drosophila melanogaster ovary cDNA library using KOD Hot Start DNA Polymerase (Merck Millipore, 71086-4) and cloned into overexpression vectors under act5 promoter with either N-terminal or C-terminal FLAG tags using Gibson Assembly® Master Mix (NEB ...
-
bioRxiv - Biochemistry 2024Quote: ... 20% sucrose (Merck, Darmstadt, DE), 0.4 mg/ml lysozyme ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... we used anti-Tph (1:250, sheep polyclonal, Merck Millipore, AB1541) and anti-GFP (1:500 ...
-
bioRxiv - Biochemistry 2024Quote: ... The chromatographic separation was performed on an Agilent Infinity II 1290 HPLC system using a SeQuant ZIC-pHILIC column (150 × 2.1 mm, 5 μm particle size, peek coated, Merck) connected to a guard column of similar specificity (20 × 2.1 mm ...
-
bioRxiv - Biochemistry 2024Quote: LPS with covalently conjugated Alexa Flour 594 was purchased from Merck (cat. No. L23353). A cysteine mutant of HU ...
-
bioRxiv - Biochemistry 2024Quote: ... The loading reaction (480 µL) was spun in a pre-rinsed Amicon Ultra 0.5 mL Centrifugal Filter with 100 kDa MWCO (Merck Millipore, 6 000 g, 6 min). After two washes with water (480 µL ...
-
bioRxiv - Biochemistry 2024Quote: 4WJ DNA was generated in vitro by annealing of four different oligos (purchased from Merck) in equimolar amounts to create a Holliday junction–like (cruciform ...
-
bioRxiv - Biochemistry 2024Quote: Cells were harvested by centrifugation at 2000 x g for 15 minutes at RT and resuspended in Lysis Buffer (1X BugBuster® Protein Extraction Reagent (Merck-Millipore), 20 mM Tris ...
-
bioRxiv - Biochemistry 2024Quote: ... ammonium acetate and methanol were purchased from Merck (Missouri, USA). Serum ...
-
bioRxiv - Biochemistry 2024Quote: ... supplied with one tablet of ethylenediaminetetraacetic acid free SigmaFAST protease inhibitor cocktail tablet (Merck KGaA, Darmstadt, Germany) per 100 ml buffer ...
-
bioRxiv - Bioengineering 2024Quote: ... We performed gel electrophoresis on a 2% agarose gel with 10 µl 10 mg/mL ethidium bromide (EtBr) submerged in 1.5L 1X TBE with 150 µl 10 mg/mL EtBr (Sigma-Aldrich cat no. E1510; Merck KGaA, Darmstadt, Germany). Wells in the gel were loaded with 10 µl amplified DNA ...
-
bioRxiv - Pathology 2024Quote: ... Trypsin and C18 SPE cartridges were obtained from Merck (KGaA, Darmstadt, Germany). The EV1109 column was obtained from Evosep (Evosep Biosystems ...
-
bioRxiv - Bioengineering 2024Quote: ... followed by 10 min of crosslinking in a 2% (w/v) calcium chloride (CaCl2; Merck, Germany) solution at RT ...
-
bioRxiv - Bioengineering 2024Quote: ... 2 μM propidium iodide (PI; Merck, Germany) for dead cell staining ...
-
bioRxiv - Bioengineering 2024Quote: ... and anti- synaptophysin antibody (1:100; Merck, Germany) in a 10% (v/v ...
-
bioRxiv - Biochemistry 2024Quote: ... and 500 nM Smoothened Agonist (SAG, from Merck Millipore) for 4 days ...
-
bioRxiv - Biochemistry 2024Quote: ... and concentrated to ∼ 10 mg/mL (as judged by the extinction coefficient χ410= 213 mM-1cm-1) using Amicon 50K MWCO centrifugal concentrators (Merck). Dimeric NmqNOR was immediately used to make grids following SEC ...
-
bioRxiv - Biochemistry 2024Quote: ... and nicotinamide adenine dinucleotide (NADH) was purchased from Merck (Madrid, Spain).
-
bioRxiv - Biochemistry 2024Quote: ... and Tris-HCl were purchased from Merck (Madrid, Spain). Polyethylene glycol (PEG ...
-
bioRxiv - Biochemistry 2024Quote: ... wells were incubated with the NCAM specific monoclonal antibody OB11 (Merck, Cat.# C9672, mouse IgG1; 1:1000), followed by horseradish peroxidase (HRP)-conjugated anti-mouse IgG1 (Jackson Cat.# 115-035-205 ...
-
bioRxiv - Immunology 2024Quote: ... for 25 min in the dark at RT followed by a second stage blocking using 1M sulphuric acid (Merck, 1.00731.1000). Optical density (OD ...
-
bioRxiv - Microbiology 2024Quote: ... the OD600 of culture samples was determined on a photospectrometer (Merck Chemicals GmbH, Darmstadt, Germany).
-
bioRxiv - Neuroscience 2024Quote: ... and 20% fetal bovine serum (F2442-100ML, Merck KGaA, Darmstadt, Germany), with the addition of 50 μg/ml streptomycin ...
-
bioRxiv - Neuroscience 2024Quote: Samples of 2mm diameter retinal punches were collected from donor eyes (Supplementary Figure 1A) and homogenized on ice using radio-immunoprecipitation assay (RIPA) buffer (#900000064244, Merck) supplied with phosphatase and protease inhibitor (1:100 ...
-
bioRxiv - Neuroscience 2024Quote: ... The culture medium contained 80% Drosophila Schneider’s insect medium (S0146-100ML, Merck KGaA, Darmstadt, Germany) and 20% fetal bovine serum (F2442-100ML ...
-
bioRxiv - Plant Biology 2024Quote: ... Fab fragments (Merck). Signals were detected using CDP-Star according to the manufacturer’s instructions (Roche Diagnostics).
-
bioRxiv - Plant Biology 2024Quote: ... the membrane was UV crosslinked and hybridized for 16h with a digoxygenin (DIG)- labelled probe specific for the HygR gene (JLF_Ox41 and JLF_Ox42, Table S2) in DIG Easy Hyb solution (Merck). Subsequent steps included stringency washes to remove any spurious annealing or unbound probe ...
-
bioRxiv - Pathology 2024Quote: ... Purified virions and vesicles were shipped in 100% methanol (Merck) on dry ice to IRD ...
-
bioRxiv - Neuroscience 2024Quote: ... Sodium dodecyl sulphate (SDS) was purchased from Merck (Darmstadt, Germany). Bovine Serum Albumin (BSA) ...
-
bioRxiv - Neuroscience 2024Quote: ... MAP2 (Merck Millipore, Mab378, 1:250), MyoD (DSHB ...
-
bioRxiv - Genetics 2024Quote: ... Proteins were then separated using 10% SDS-PAGE and transferred to PVDF membranes (Merck Millipore). PVDF membranes were then blocked by 5% BSA ...
-
bioRxiv - Immunology 2024Quote: ... MultiScreen 96-well filtration plates (Merck Millipore) were coated with LPS or porins (5 µg/ml ...
-
bioRxiv - Neuroscience 2024Quote: ... and afferent fibres (neurofilament H; chicken anti-neurofilament H, 1:500, Merck), were used for immunofluorescent labelling ...
-
bioRxiv - Pathology 2024Quote: ... CD31 (2H8, Merck Millipore), CD31 (MEC13.3 ...
-
bioRxiv - Developmental Biology 2024Quote: ... All sections from each sample were homogenised using a 7 ml glass Dounce tissue grinder set (Merck) with 8–10 strokes of a tight pestle (B ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with one or more of the following primary antibodies in blocking buffer overnight at 4°C (rabbit anti-TH, 1:2000; AB152, Merck-Millipore ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... and then loaded on a silica gel 60 column (Merck, Darmstadt, Germany). Carotenes were eluted with hexane ...
-
bioRxiv - Neuroscience 2024Quote: ... and a second PDMS gasket (Merck, GBL-103250-10EA) was stacked on the coverslip before introducing 3 µL of complimentary imaging strand (TGGTGGT-cy3B ...
-
bioRxiv - Neuroscience 2024Quote: ... the culture was stimulated by substituting one third of the medium with L929 medium (L929 mouse fibroblast cells had previously been plated in T175 cm2 cell culture flasks (Corning, Merck) with 100 mL culture medium (DMEM ...
-
bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used: chicken anti-GFAP 1:500 (ab5541, Merck Millipore, Darmstadt, Germany), mouse anti-AT8 1:1000 (ab5541 ...
-
Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... or mouse monoclonal anti-GAPDH (#MAB374; Merck Millipore). After washes ...
-
bioRxiv - Plant Biology 2024Quote: ... A standard curve was prepared using cyanidin-3-O-glucoside (Merck KGaA, Darmstadt, Germany) for calculating Anth content per unit leaf FW.
-
bioRxiv - Developmental Biology 2024Quote: ... samples were stored at 4 °C overnight in HypoThermosol preservation solution (Merck). Tissue was first minced in a tissue culture dish using scalpel ...
-
bioRxiv - Developmental Biology 2024Quote: A digestion buffer comprising of 0.05 mg/mL activated DNAse I (DNase I with HBSS in a 1:1 ratio, Merck), 10 mg/mL Collagenase (Thermo Fisher ...
-
bioRxiv - Neuroscience 2024Quote: ... Membranes were visualized using Fusion Fx (Vilber Lourmat, Marne-la-Vallée, France) with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, Billerica, MA, USA). Protein levels were quantified using ImageJ and normalized to the β-actin levels.
-
bioRxiv - Neuroscience 2024Quote: ... Sections were blocked in 10% normal goat serum in TBS-T for one hour at room temperature and then incubated in the following primary antibodies suspended in blocking buffer overnight at 4°C: pS129 (El-Agnaf laboratory, 1µg/ml 27) and 5G4 (MABN389, Merck, 2µg/ml) combined with either MGO-aSyn (1µg/ml ...
-
bioRxiv - Biochemistry 2024Quote: ... Supernatant was discarded and pellet was washed once with 1 mL ice-cold 70% ethanol (Merck, 818760.2500) by centrifugation at 13,000 x g for 15 min at 4°C ...
-
bioRxiv - Biochemistry 2024Quote: ... Blocking with 5% bovine serum albumin (Merck, 126575) was used for rabbit anti-LC3B-I/II (1:3000 ...
-
bioRxiv - Biochemistry 2024Quote: ... Excess DTT and imidazole were removed on a PD-10 desalting column (Merck), and the reduced proteins were eluted with 3.5 mL ice-cold assay buffer containing 100 mM NaxHyPO4 ...