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Citations for Merck :
2501 - 2550 of 5053 citations for 8 chloro 10 3 dimethylamino propyl phenothiazin 1 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The cultured cells were collected by centrifugation (8,000 × g for 10 min at 4 °C) and suspended in BugBuster Protein Extraction Reagent according to the manufacturer’s procedures (Merck Millipore). The supernatant was collected by centrifugation (12,000 × g for 10 min at 4 °C) ...
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bioRxiv - Biochemistry 2020Quote: ... concentrated and buffer exchanged to TE (10 mM Tris pH 7.5, 0.1 mM EDTA) using a 10,000 MWCO concentrator (Merck Pte. Ltd, Singapore).
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bioRxiv - Cell Biology 2021Quote: ... the coverslips were mounted on microscopy slides with Mowiol (0.1 M tris-HCl pH 8.5, 25% glycerol, 10% Mowiol (Merck Millipore, 81381). The samples were imaged using a Zeiss Axioscope A1 microscope with a 40x objective.
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bioRxiv - Cell Biology 2021Quote: ... cut axially using a size 10 surgical scalpel into 1mm3 pieces and incubated at 37°C for 45 mins in Liberase (Merck) and 10ul/ml DNAse I (Thermo Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... VLP samples were separated by 10% or 14% SDS-PAGE later blotted to PVDF membrane (Immobilon®-FL, Merck Millipore) and blocked overnight with ODYSSEY® blocking buffer ...
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bioRxiv - Biophysics 2021Quote: ... All the fractions containing the monomeric protein were pooled together and concentrated using amicon 10 k MWCO centrifugal filtration devices (Merck). Protein purity was determined by aRP to be 88.6% (Supplementary Figure 8) ...
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bioRxiv - Plant Biology 2021Quote: ... surface sterilized with 10% (v/v) bleach solution and plated on agar plates containing one-half strength Murashige and Skoog (MS) salts (Merck) pH 5.7 ...
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bioRxiv - Neuroscience 2020Quote: ... two custom-made Teflon containers of 5 mm diameter were filled with 10 μl of odor substance (n-amylacetate, AM; CAS: 628-63-7, Merck, Darmstadt ...
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bioRxiv - Molecular Biology 2019Quote: ... The resulting glycoconjugate was washed and concentrated with deionized water using 10 kDa centrifugal filter units (Merck Millipore, Tullagreen, Ireland).
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bioRxiv - Microbiology 2021Quote: ... the cell suspensions were spun at 10,000 x g for 10 minutes and bacterial pellets were lysed by addition of BugBuster Master Mix (Merck Millipore) for 25 minutes at room temperature with gentle agitation ...
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bioRxiv - Microbiology 2021Quote: ... spun at 10,000 x g for 10 minutes and bacterial pellets lysed by addition of BugBuster Master Mix (Merck Millipore) for 25 minutes at room temperature with gentle agitation ...
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bioRxiv - Molecular Biology 2020Quote: ... 30~50 μg of protein samples were separated on a 10% SDS-PAGE gel and transferred to a PVDF membrane (Merck) using an Amersham semi-dry transfer system ...
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bioRxiv - Microbiology 2020Quote: ... The crude fractions were dissolved in EA (10 mg/ml) and 10 µl from each were spotted onto 20 × 20 cm TLC plates (Silica gel 60 F254, Merck) with at least one cm between the spots and placed in a binary mobile phase consisting of Chloroform ...
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bioRxiv - Microbiology 2020Quote: ... Twenty µl of the EA fraction (10 mg/ml) were spotted onto 20 × 20 cm TLC plates (Silica gel 60 F254, Merck) with at least one cm between the spots and placed in a quaternary mobile phase consisting of Benzene ...
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bioRxiv - Microbiology 2020Quote: ... all supernatant was removed and the cells were fixed with 10% formalin for 15 min at room temperature and stained with 100 µg/ml Hoechst 33342 (Merck) for 30 min in the dark at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... were assessed by analytical gel filtration using an Akta FPLC UPC-900 equipped with a Superose 6 Increase 10/300 GL column (Merck). Typically ...
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bioRxiv - Cancer Biology 2020Quote: ... MCF7 cells were transduced with lentiviral particles at 75% confluency in the presence of 10 μg/ml polybrene (Merck, Germany). 24h after transduction virus containing medium was replaced with selection medium for the respective expression constructs to start the selection ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 cells were differentiated to macrophages by complementing the media with 10 ng/ml phorbol 12-myristate 13-acetate (#P8139, Merck) for seven days ...
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bioRxiv - Biophysics 2021Quote: ... We discarded the supernatant and carefully resuspended the pellet in 800 µL M2B-taxol (M2B buffer supplemented with 10 µM taxol (T7402, Merck)) ...
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bioRxiv - Cancer Biology 2021Quote: Urine samples were first adjusted to pH 8.0 using 500 mM ABC supplemented with EDTA-free protease inhibitor and then filtered through an Amicon Ultra-0.5 mL Centrifugal Filter Device (10 kDa, Merck Millipore). Collection of the retentate was performed by flipping the filter device upside-down and conducting centrifugation at 1,000g for 2 min ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions that were highly enriched for Redβ177 were pooled and concentrated in a 10 kDa MWCO centrifugal concentrator (Merck Millipore) to a total volume of <2 ml ...
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bioRxiv - Biochemistry 2022Quote: ... An assay volume of 150 μl was dispensed into a Hellma 10 x 2 mm Suprasil quartz cuvette (Merck #Z802778). For double-stranded substrates ...
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bioRxiv - Microbiology 2022Quote: Supernatants harvested from infected Rhileki cells were centrifuged at 3,500 rpm and precipitated with 10% polyethylene glycol 8000 (Merck, Germany) overnight in 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Ninety μl of cell lysates were mixed with 10 μl of lysis buffer containing 0.1 μl benzonase (Merck Millipore, 70664) and further incubated on ice for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... was repeatedly washed with PBS supplemented with protease inhibitor cocktail and reconcentrated using an Amicon® Ultra-0.5 device with 10 K MWCO (Merck) until residual urea was diluted below 10 mM ...
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bioRxiv - Cell Biology 2022Quote: ... 10 mM stock solution was prepared by dissolving ROCKi powder in 100% dimethyl sulfoxide (DMSO, Merck Life Science, UK, #D8418) and filtered through 0.22µM syringe filters ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were loaded and resolved using a 10% SDS-PAGE gel and the separated proteins were transferred onto a PVDF membrane (IPVH00010, Immobilon-P; Merck). Blocking was performed using 5% Skimmed milk (70166 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... while the rat abdominal aortic rings (15 mg wet mass/mL) were pre-incubated for 10 min in Medium 199 (Merck). The examined enzyme reaction started after pipetting the tracer substrate 1-14C-AA (3.7 kBq ...
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bioRxiv - Microbiology 2020Quote: Stock solutions of crude extracts and fractions were prepared at a concentration of 100 mg/ml in 10% dimethyl sulfoxide (DMSO - Merck).
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bioRxiv - Microbiology 2019Quote: ... pH 7.4) through multiple dilution and centrifugation steps using Amicon Ultra-15 centrifugal filters (10 kDa cuttoff, Merck Millipore, Germany). Protein purity was estimated with SDS-PAGE and the concentration determined with the Pierce BCA assay kit (Thermo Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... Fractions containing protein were pooled and concentrated using Amicon Ultra-15 centrifugal filter unit (10-kDa molecular mass cut-off; Merck) until total protein concentration of 10 mg·mL−1 was reached ...
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bioRxiv - Biophysics 2019Quote: ... All the fractions containing the monomeric protein were pooled together and concentrated by using amicon 10 k MWCO centrifugal filtration devices (Merck).
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bioRxiv - Biochemistry 2019Quote: ... lipid extracts were also spotted on HPTLC precoated silica gel glass plates 60F254 (20×10 cm; 1.05642.0001; E. Merck, Darmstadt, Germany) using a Camag autosampler ATS4 sample applicator (Camag ...
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bioRxiv - Synthetic Biology 2019Quote: ... pH 8.5 (UA buffer) and loaded onto a 10 kDa molecular weight cut-off (MWCO) centrifugal filter (Merck Millipore, Australia) and centrifuged at 20,800 x g for 15 min at 22°C ...
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bioRxiv - Microbiology 2019Quote: Proteins in cell lysates were separated in a 10% SDS-PAGE and transferred to Immobilon NC (Millipore Merck, Darmstadt, Germany) membranes ...
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bioRxiv - Microbiology 2020Quote: ... washed with PBS at room temperature before permeabilized for 30 min in PBS containing 0.1% Triton X-100 and 10% normal goat serum and incubated overnight at 4 °C in PBS containing 10% normal goat serum and primary antibodies: anti-Cytokeratin 14 (K14) antibody (SP53; Merck), anti-Neurofilament heavy polypeptide (NF200 ...
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bioRxiv - Cell Biology 2020Quote: ... Fractions 7-9 were individually collected (500 μL/fraction) and concentrated by Amicon Ultra-0.5 centrifugal filter unit with 10 kDa membrane (Merck Millipore) with 14,000 x g at 4 °C for 8 min centrifugation to have final volume less than 100 μL ...
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bioRxiv - Bioengineering 2019Quote: ... 1 mM EDTA) and glycerol (2.5%, v/v) were mixed and polymerized by the addition of fresh 10% APS solution (Merck Millipore) (final concentration 10% ...
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bioRxiv - Molecular Biology 2020Quote: ... GndHCl containing samples were subjected to a buffer exchange regime using Microcon-10 kDa centrifugal filter units (Merck, Darmstadt, Germany). Centrifugation (14’000x g ...
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bioRxiv - Microbiology 2019Quote: ... 100 μL of the faeces mixture was added to 10 mL of Selenite Broth (19 g/L selenite broth base, Merck, UK ...
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bioRxiv - Biophysics 2019Quote: ... 1 μM EDTA for PsaA) under oxygen-free conditions obtained utilizing an oxygen-scavenging system supplemented with 10 mM of Trolox (Merck)71 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed with 4% (vol/vol) paraformaldehyde for 10 min at RT and permeabilized with 0.3% Tween 20 (#822184, Merck Millipore). All the primary and secondary antibodies were diluted in blocking buffer consisting of 0.3 M glycine ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... all proteins were centrifuged (16 000 g, 10 min, 20°C) and filtered (0.1 μm Ultrafree-MC filter, Merck Millipore). Proper performance of the MALS detectors was validated with bovine serum albumin ...
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bioRxiv - Microbiology 2019Quote: ... and sulfide were measured from the supernatant after centrifugation (10 min at 5000 rpm) using specific colorimetric chemical kits provided by Merck AG (Zug ...
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bioRxiv - Immunology 2021Quote: All samples for CD-MS measurements were first buffer exchanged into 500 mM ammonium acetate solution (pH 7.5) using Amicon 10 kDa MWCO centrifugal filters (Merck Millipore), unless otherwise stated ...
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bioRxiv - Neuroscience 2022Quote: ... Six rats received an infusion of 1.5 μL (n = 4) or 3.0 μL (n = 2) of 10% glycerol (Merck, Darmstadt, Germany) in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein (10 µg) were analyzed by SDS-PAGE and transferred to Immobilon-FL PVDF membranes (Merck Millipore) using Pierce™ 1-step transfer buffer (Thermo ...
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bioRxiv - Biochemistry 2022Quote: ... The MTF1 protein eluent was collected and concentrated using 10 kDa MW cut-off Amicon ultra-centrifugal filter (Merck Millipore) and stored at -80°C ...
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bioRxiv - Cancer Biology 2022Quote: Panc02 pancreatic tumours were flash frozen after excision and approximately 10 mg of tumour mass was homogeneised in RIPA lysis buffer (Merck) using a FastPrep-24 5G homogeneiser (MP Biomedicals ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting cell pellet was freeze-thawed thrice and re-suspended to 10% (w/v) in a lysis buffer containing 5% (v/v) Polysorbate 80 and 20 U/mL benzonase (Merck). After 1h incubation at 37°C ...