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Citations for Merck :
2201 - 2250 of 4853 citations for 6 Amino 3 morpholin 4 ylmethyl indazole 1 carboxylic acid tert butyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The cortices were homogenized mechanically in PBS by pipetting up and down with a 5mL plastic pipette, centrifuged (500 x g, 7 min, 4 °C) and resuspended in DMEM (Sigma-Aldrich, Merck, Switzerland) containing 5% FCS (Biochrom ...
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bioRxiv - Biophysics 2019Quote: ... Purified PCV2 VLPs were concentrated and buffer exchanged to phosphate buffered saline (PBS) using Amicon Utra-4 centrifugal filter devices (Merck Millipore, MA). PCV2 VLP samples were stored in 50-100 µl aliquots at −80°C.
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bioRxiv - Biochemistry 2020Quote: ... concentration and buffer exchange to TE with an Amicon Ultra-4 centrifuge filter unit (30 kDa cut-off, Merck Millipore, Cat#UFC803024).
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bioRxiv - Microbiology 2019Quote: ... 100 μL of the faeces mixture was added to 10 mL of Selenite Broth (19 g/L selenite broth base, Merck, UK, 70153-500G and 4 g/L sodium hydrogen selenite, Merck 1.06340-50G) and incubated overnight at 37°C with shaking at 220 rpm ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
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bioRxiv - Immunology 2021Quote: ... These fractions were then pooled and buffer exchanged using SnakeSkin™ dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096). The clinically relevant mAbs tested here were in-house produced versions of ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein containing fractions were pooled and buffer exchanged using SnakeSkin™ dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096), as described previously (5).
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bioRxiv - Biophysics 2020Quote: Upon deposition the samples were washed 2 times 15 minutes with PBS prior to overnight fixation with 4% paraformaldehyde (PFA, Merck, Darmstadt, Germany). Washing steps and incubation took place under agitation at 4 °C unless stated otherwise ...
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bioRxiv - Biochemistry 2022Quote: Collected samples from the first main peak were concentrated by ultrafiltration on 10K Amicon® Ultra-4 Centrifugal Filters Units (Merck Millipore) in a swinging-bucket rotor 4 000 g ...
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bioRxiv - Biochemistry 2022Quote: ... The TEV cleaved complex was concentrated to around 500 μl using a 4 ml Amicon® Ultra centrifugal filter concentrator (Merck Millipore) with a membrane molecular weight cut off 10 kDa ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were administered a lethal dose of sodium pentobarbital and transcardially perfused with Ringer’s solution followed by paraformaldehyde (PFA, 4 %, Merck Millipore, Darmstadt, Germany) in 125 mM phosphate buffer (PB) ...
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bioRxiv - Neuroscience 2022Quote: ... Sonicated sample was diluted with 3.5 ml PBS and concentrated to 500 μl by diafiltration using an Amicon® Ultra-4 Centrifugal Filter Unit with 30 KDa cutoff (Merck UFC803024) to remove traces of sarkosyl ...
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bioRxiv - Neuroscience 2022Quote: ... and concentrated to 300 ul by diafiltration using an Amicon® Ultra-4 Centrifugal Filter Unit with 30 kDa cutoff (Merck UFC803024) to remove the potentially unincorporated dye ...
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bioRxiv - Synthetic Biology 2024Quote: ... Protein containing fractions were pooled and buffer exchanged using SnakeSkin dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096), as described previously (44).
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bioRxiv - Microbiology 2024Quote: ... the same dilutions were also asdded to Eosin Methylene Blue Agar and MacConkey Agar both supplemented with cefotaxime (4 mg/L) (Merck, Darmstadt, Germany) for the Extended-spectrum beta-lactamase phenotype (37) ...
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bioRxiv - Plant Biology 2024Quote: The following chemicals were used in the DEEP-Clear (Pende et al., 2020) adapted version to plant tissues: PFA (paraformaldehyde) (CAS no. 30525-89-4, Merck, http://www.merck.com/), xylitol (CAS no ...
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bioRxiv - Microbiology 2024Quote: ... The flow-through was further concentrated from 10 ml volume in an Amicon® Ultra-4 Centrifugal Filter 50 kDa unit (Merck Millipore) and stored at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: GNAO1+/G203R#2 cells at the stage of neuronal rosettes (30 day of differentiation) were fixed in 4% paraformaldehyde solution (Merck Life Sciences) for 15 min at room temperature and washed thrice with 1X PBS (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Two sets of four EV-rich fractions (7–12 and 16-22) were pooled and concentrated using Amicon Ultra-4 10 kDa centrifugal filter device (Merck Millipore, USA). Alternatively ...
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bioRxiv - Bioengineering 2023Quote: ... Conditioned medium was then centrifuged at 1500 rpm for 5 min and concentrated 30x times in Amicon® Ultra-4 Centrifugal Filter Unit with 30 kDa MWCO cut-off (Merck Millipore). Concentrated conditioned medium was then filtered using 0.2 μm filters and frozen until use.
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bioRxiv - Cancer Biology 2024Quote: ... The cells were washed in 1x PBS and subsequently mixed with 100 μL 4% SDS (Carl Roth GmbH, Karlsruhe, Germany) and 2% beta-Mercaptoethanol (Merck, Darmstadt, Germany) in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... and loaded in 4–12% NuPAGE Bis-Tris gel (Fisher, 10247002) for subsequent electrophoresis and transference to nitrocellulose membrane (Merck-Sigma, GE10600003). Precipitated RNA was analyzed by autoradiography and FLAG-PTBP protein levels were measured by western blot.
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bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was filtrated through a filter paper (Whatman No. 4, Whatman plc, Kent, UK) and a membrane filter (MF-milliporeTM 0.22 μm, Merck KGaA, Darmstadt, Germany). The solution was cleaned up through a Waters Sep-Pak C18 column (Waters ...
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bioRxiv - Biophysics 2023Quote: ... containing 0.3□mM biotin and concentrated at +4 °C to 25 μl volume using Amicon ®Ultra 10 kDa MWCO centrifugal filters (Merck Millipore).
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bioRxiv - Biochemistry 2023Quote: Total cell lysates of HEK293 cells stably expressing C-terminally Flag M2-tagged SIRT4 were incubated overnight at 4°C with anti-Flag M2 agarose beads (Merck, Darmstadt, Germany). The beads were washed three times with washing buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue homogenisation and western blotting was performed with 50 μg of total protein as previously described.15 Membranes were incubated overnight with gentle agitation at 4 °C with the MSH3 antibody (Merck MSH3MAB(1F6)) in 5% milk PSB-T (phosphate buffer saline (PBS) ...
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bioRxiv - Bioengineering 2023Quote: ... at 40,000 xg for 20 min at 4°C and the supernatant was filtered through a 0.45 µm Millex® syringe filter (Merck, Darmstadt, Germany). Clarified lysate was loaded onto a nickel (II ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM FBP (Merck) (where applicable) ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μM dexamethasone (Merck), 5 μg/mL insulin (Merck ...
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bioRxiv - Neuroscience 2022Quote: ... 1 NADPH (Merck-Millipore), and 0.2% Triton X-100 ...
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bioRxiv - Immunology 2021Quote: ... and 1 % methylcellulose (Merck). The PRRSV-2 16CB02 isolate (referred to as PRRSV-2 ...
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bioRxiv - Molecular Biology 2019Quote: ... Cyclopamine (1 μM, Merck) was added from day 2 to day 10 of differentiation ...
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bioRxiv - Biophysics 2019Quote: ... 1 mM PMSF (Merck) and a protease inhibitor cocktail (complete™ ...
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bioRxiv - Cell Biology 2021Quote: ... and 1% NH4OH (Merck) right before infusion into the MS at a flow rate of 10 μL/min ...
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bioRxiv - Physiology 2022Quote: ... or Mdivi-1 (Merck) at the times and concentrations indicated in the figure’s legends.
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bioRxiv - Cancer Biology 2022Quote: ... 1 µM staurosporine (Merck), vehicle control or compounds from the small molecule library (Supplementary Table 4 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 mM DTT (MERCK, Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Wiskostatin (Merck, 1 μM), CCL19 (Peprotech ...
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bioRxiv - Bioengineering 2022Quote: ... 1× ITS (Merck, I3146), 500 µM monothioglycerol (Sigma–Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... 1% pen/strep (Merck), 1% non-essential amino acids (Gibco ...
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bioRxiv - Neuroscience 2019Quote: ... 1 mM NaHCO3 (Merck).
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bioRxiv - Neuroscience 2019Quote: ... Reelin (1:400, Merck Cat#MAB5364 ...
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bioRxiv - Developmental Biology 2020Quote: ... pH 7.4 (Carl Roth),125 mM KCl (Merck),1 mM MgCl2 (Merck),1 mM EGTA/KOH pH 8.0 (Carl Roth),5% glycerol (Merck),1% NP-40 (Nonidet P 40 Substitute ...
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bioRxiv - Pathology 2021Quote: ... 1 mM EDTA (Merck), 1% Triton X-100 ...
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bioRxiv - Cell Biology 2021Quote: ... 1% sucrose (Merck # 84100) and 2.5□mM morpholinoethanesulfonic acid (Euromedex # EU0033 ...
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bioRxiv - Immunology 2021Quote: ... 1 % penicillin/streptomycin (Merck) and 1 % methylcellulose (Merck) ...
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bioRxiv - Immunology 2020Quote: ... 1% NP-40 (Merck), 1m DDT (Merck) ...
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bioRxiv - Neuroscience 2022Quote: ... and 1% PenStrep (Merck). Human SH-SY5Y neuroblastoma cells were cultured in DMEM/F-12 (ThermoFisher) ...