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Citations for Merck :
1851 - 1900 of 2050 citations for Rat Interleukin 10 Receptor Beta IL10Rb ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Global citrullination was detected with the antimodified citrulline (AMC) Detection Kit (cat. 17-347B, Merck) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2023Quote: ... yolk sac or tail of embryos with the REDExtract-N-Amp Tissue PCR kit (Merck) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... DNA purification was performed using a SpinPrep™ PCR Clean-up Kit (Merck Millipore, Germany). DNA fragments were assayed by qRT-PCR using the primer sequences listed below ...
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bioRxiv - Immunology 2023Quote: ... they were detected for intermolecular binding using Duolink flow PLA detection kit (Green) (Sigma/Merck) with a reaction time of 100 minutes for post-mouse/rabbit probe linking amplification ...
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bioRxiv - Biochemistry 2024Quote: The mitochondrial membrane potential determination was performed employing the mitochondrial membrane potential kit (Merck; MAK159). The cells were grown in 5 mL SC medium supplemented with glucose (0.1% ...
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bioRxiv - Biochemistry 2024Quote: The ADP/ATP ratio was quantified employing the ADP/ATP ratio assay kit (Merck; MAK135). S ...
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bioRxiv - Physiology 2024Quote: Total protein carbonylation was detected by immunoblotting using the OxyBlot Protein Oxidation Detection kit (Merck) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and resuspended in 60 μl same with 1 μl lyticase (17 U/ μl in 10 mM KPO4 pH7, 50% glycerol, Merck >2000 U/mg L2524). Samples were heated to 50°C for 1-10 min before addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594) ...
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bioRxiv - Molecular Biology 2021Quote: ... and resuspended in 60 μl PFGE wash buffer containing 1 μl lyticase (17 U/μl 10 mM KPO4 pH 7, 50% glycerol Merck L2524 >2000 U/mg) then incubated for 10 min at 50°C ...
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bioRxiv - Microbiology 2020Quote: ... The internal standard was 1 μL of methyl heneicosanoate (10 mg/mL) and Bacterial acid methyl ester (BAME) mix (Merck-Millipore, Burlington, MA, USA) was used to identify the each peak of fatty acids and analytical standards for each fatty acid were used for quantification.
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bioRxiv - Cancer Biology 2021Quote: ... Puromycin incorporation is a proxy for the mRNA translation rate in vitro and was measured by western blotting using an anti-puromycin antibody (MABE343, clone 12D10, Merck Millipore, 1:10 000). The antibody was diluted in Milk/TBST (5% non-fat dry milk in TBS with 0.1% Tween20) ...
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bioRxiv - Genetics 2022Quote: ... 50 μg of protein was used for gel electrophoresis in 10-14% SDS-PAGE gels and transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA). After blocking in 5% defatted milk ...
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bioRxiv - Cell Biology 2022Quote: ... The eluate was concentrated down to 1.5 mL using an Amicon Ultra Centrifuge Filter unit (30 kDa cutoff for GST-UtrCH-sfGFP and 10 kDa cutoff for GST-LifeAct-sfGFP; Merck, UFC903024 and UFC901024, respectively). The GST tag was removed by digestion with 8 units/mL PreScission Protease (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... [11] were maintained in Dulbecco’s modified Eagle medium (DMEM) (Nacalai Tesque, Inc., Kyoto, Japan) supplemented with 10% fetal bovine serum (Sigma-Aldrich, Merck, Kenilworth, New Jersey, USA) and 1 mg/ml of G418 (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Equal amounts of protein were then separated through 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore Ltd., Tullagreen, Ireland). Membranes were blocked in 5% nonfat dry milk in 1× TBST for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed 3 times in TBS before being probed with protein A-peroxidase (Merck, 1:50000 in 10% Skimmed Milk in TBS). The membrane was incubated with 1x LumiGLO® / 1x Peroxidase (CellSignal ...
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bioRxiv - Immunology 2023Quote: ... Eluted proteins were buffer exchanged into PBS and then concentrated down using Amicon® 100 kDa or 10 kDa Ultra-15 Centrifugal Filter Units (Merck Millipore UFC9100 & UFC9010), respectively ...
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bioRxiv - Developmental Biology 2022Quote: ... MII oocytes were activated for 6 h in Ca-free α-MEM medium containing 10 mM SrCl2 and 5 μM Latrunculin B (cat. no. 428020, Merck Millipore, Darmstadt, Germany). Following activation ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged to remove cell debris and concentrated with Amicon Ultra-15 Centrifugal Filters (10 kDa molecular weight cut-off; Merck Millipore Ltd, Carrigtwohill, Ireland). Aliquots were stored at −80°C until usage.
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bioRxiv - Biochemistry 2024Quote: ... Sephacryl S-200 and Sephadex G-25 (PD-10 columns) were from Amer-sham-Pharmacia (Cytiva, now purchasable from Merck Life Sciences, Madrid, Spain). DEAE-cellulose DE52 was from Whatman (now purchasable from Merck Life Sciences ...
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bioRxiv - Immunology 2024Quote: ... the differentiating cells were transduced with mVDR and Gfi1-expressing retrovirus particles in the presence of 10 µg/mL of Polybrene transfection reagent (Merck Millipore, Burlington, MA, USA). These cells were then subsequently expanded on day 3 and finally harvested for flow cytometry on day 5 ...
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bioRxiv - Biochemistry 2021Quote: ... Apoptotic cells were stained using ApopTag Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore, MA, USA) according to manufacturers instructions.
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bioRxiv - Molecular Biology 2022Quote: ... RNA was extracted using a Spectrum Plant Total RNA kit (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Physiology 2022Quote: ... To detect total protein carbonylation by immunoblot we used the OxyBlot Protein Oxidation Detection kit (Merck) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Cell lysis and membrane protein extraction was performed using the ProteoExtract Native Membrane kit (Merck Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Secondary antibody incubation was followed by 0.1% TBST wash and developed using an ECL kit (Merck). Densitometry analysis was used for quantitation of protein expression levels using Image J ...
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bioRxiv - Microbiology 2020Quote: ... Cell death was detected by Apoptag® Red in situ apoptosis detection kit (Merck, catalog # S7165) which labels apoptotic cells ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... Immunoprecipitation of m6A-modified transcripts was carried out with Magna MeRIP™ m6A kit (Merck-Millipore) following the manual ...
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bioRxiv - Cancer Biology 2022Quote: Cell migration was assessed using the QCM Chemotaxis Cell Migration Colorimetric Assay kit (ECM506, Merck, USA). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... The live/dead cells were stained with the Calcein-AM/PI Kit (Merck, 04511-1KT-F) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... and LX-2 cells were labeled with a PKH67 Green Fluorescent Cell Linker Kit (Merck, PKH67GL) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: ... The full-length MDM2 coding sequence was isolated from Addgene plasmid #16233 (pcDNA3 FRT MDM2) by PCR using the KOD hot start polymerase kit (Merck #71086), using primers that included a 5’ BamHI restriction site ...
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bioRxiv - Cell Biology 2024Quote: ... HRP-mediated substrate development was performed using Amersham detection system (ECL Western Blot detection kit, Merck). Blots were imaged using ChemiDoc™ MP Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... medium was further exchanged with FibroGRO™ Complete Media Kit for Culture of Human Fibroblasts (Merck), consisting of a fibroblasts-specific basal media supplemented with P/S (0.5%) ...
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bioRxiv - Microbiology 2023Quote: Apoptosis was detected using the ApopTag Peroxidase In Situ Apoptosis Detection Kit (Merck Catalogue No. S7100) as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... All cell lines were tested every 6-12 months either by PCR (LookOut kit, Sigma/Merck) or Mycostrips (Invivogen ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was isolated using the SpectrumTM Plant Total RNA Kit (Sigma Aldrich, Merck KGaA, Darmstadt, Germany). The material was stored at −70 °C until use.
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bioRxiv - Plant Biology 2023Quote: ... germinating seeds or rosette leaves using Spectrum™ Plant Total RNA Kit (Merck KgaA, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from cell pellets using the GenElute™ Bacterial Genomic DNA kit (Merck) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... gDNA was extracted from neuronal cultures with the GenEluteTM Mammalian Genomic DNA Miniprep Kit (G1N10, Merck). qPCR was performed as described above with 20 ng of gDNA in a final volume of 10 μL ...
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bioRxiv - Molecular Biology 2024Quote: PLA was performed by using Duolink In Situ Red Starter Kit Mouse/Rabbit (Merck, #DUO92101-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Infected and sham-infected animals were separated into four groups according to the treatment received: 10 μL of intramuscular vitamin B12 (Merck, Kenilworth, NJ; 6.25 mg/ kg) (N = 10 infected - vitamin B12 ...
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bioRxiv - Cell Biology 2019Quote: ... and resuspended in Lysis Buffer (Tris 10 mM pH 8, 2 mM EDTA) supplemented with Complete protease inhibitor (Roche, Merck, Sigma-Aldrich, l’Isle d’Abeau, France). After three cycles of freezing – thawing ...
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bioRxiv - Molecular Biology 2024Quote: ... the eggs were transferred into centrifuge tubes containing 1 ml XBE2 + 10 µg/ ml protease inhibitor (aprotinin Sigma A6279, leupeptin Merck 108975, pepstatin Carl Roth 2936.1) + 100 µg/ ml cytochalasin D (Sigma C8273) ...
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bioRxiv - Plant Biology 2021Quote: ... the washed seedlings were transferred into TetSee X solution (15% Na-deoxycholate, 25% urea, 10 % glycerol, 5% 2’2-thiodiethanol [Merck, Product No. 166782], 1% Triton X-100) and kept for 3 days at 4°C with daily changes of the TetSee X solution ...
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bioRxiv - Neuroscience 2020Quote: ... Bacteria were resuspended in (50mM HEPES, 250mM NaCl, 10% glycerol plus 1% Triton X-100, supplemented with protease inhibitors (Merck, Calbiochem set III, Cat. No. 539134) and subsequently sonicated ...
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bioRxiv - Microbiology 2020Quote: ... First strand cDNA preparations were diluted 1:10 with RNase-free water and subjected to RT-qPCR using SYBR Green JumpStart Taq ReadyMix (Merck Life Science UK Ltd., Gillingham, UK), with primers specified in Table S2 ...
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bioRxiv - Neuroscience 2023Quote: ... At 5 hpf embryos were placed into 10 cm Petri dishes containing E3 medium (control) and E3 medium with 1 μM VPA (Sigma-Aldrich, P4543; Merck Life Science Srl, Milan, Italy) for 24 or 48 h ...
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bioRxiv - Developmental Biology 2020Quote: ... the number of viable cells was counted using the Muse Count & Viability Assay Kit (Merck Millipore MCH100102) and run on the Muse cell analyser (Merck Millipore) ...