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Citations for Merck :
1801 - 1850 of 2050 citations for Rat Interleukin 10 Receptor Beta IL10Rb ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Suspensions were passed through a 40-µm strainer into a 50 ml tube and rinsed again in 12 ml of DMEM with 10% Fetal bovine serum (FBS) (Merck, Kenilworth New Jersey) in 15 ml tubes ...
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bioRxiv - Plant Biology 2022Quote: ... followed by a 20 min centrifugation at 4°C and 4000 rpm using 10-kD Amicon®Ultra centrifuge filter (Merck, Darmstadt, Germany) to remove free (proteinun bound ...
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bioRxiv - Biophysics 2022Quote: ... The PDMS particles were generated by vortex-mixing a solution of 1 g PDMS (10:1 w/w, base/curing agent) dispersed in 10ml of 2%w/v poly(ethylene glycol) monooleate (Merck Chemicals GmbH, Germany) aqueous solution ...
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bioRxiv - Molecular Biology 2019Quote: ... was thawed at room temperature and vortexed extensively before being concentrated to 800 µl using a 10 kDa centrifugal filter device (Centricon Plus-70, Merck Millipore, Massachusetts, USA). The concentrated urine sample was resuspended in 3.2 ml of a 50% iodixanol solution and layered on the bottom of a 17 ml Thinwall Polypropylene Tube (Beckman Coulter ...
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bioRxiv - Physiology 2019Quote: ... in 35 mm Petri dishes were incubated for 10 min in steady state E2 medium (controls) or E2 + 50 or 250 mM NaCl (Merck, #106404, Darmstadt, Germany) at 28°C under white light illumination ...
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bioRxiv - Biochemistry 2019Quote: ... Eluted protein was concentrated using Amicon Ultra-15 centrifugal filter unit with nominal molecular weight limit of 10 kDa (Merck Millipore, Burlington, MA), loaded on a HiLoad 26/600 Superdex 200 column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting cell free supernatant (CFS) was concentrated through a 10 kDa polyethersulfone membrane disc using an Amicon® Stirred Cell (Merck Millipore, Germany) to a final volume of 50 mL ...
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bioRxiv - Cell Biology 2021Quote: ... BHK-2P maintained either in Glasgow’s Minimal Essential Medium (GMEM) with 10% FBS (“line #2”) or adapted to the ACFM Cellvento™ BHK200 (Merck KGaA, Darmstadt, Germany) in two different processes (lines “#4” and “#5”) ...
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bioRxiv - Developmental Biology 2022Quote: ... The dehydrated samples were then incubated in a 1:1 mixture of propylene oxide and absolute ethanol for five minutes at RT followed by a two times 10 minutes incubation in pure propylene oxide (Sigma-Aldrich, Merck, Darmstadt, Germany), and a 30 minutes incubation in a 1:1 mixture of propylene oxide and Spurr resin (Low Viscosity Spurr Kit ...
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bioRxiv - Biochemistry 2022Quote: ... the resulting solids were resuspended in 1 mL of UP-water and desalted using centrifugal filtration (Merck, Amicon® Ultra 10 kDa filters) at 14,000 rcf for 10 minutes at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Testes were washed three times (10 min each) with PBS-T and incubated with anti-H4ac (Merck Millipore #06-598, 1/500 dilution), anti-Histones (Millipore #MABE71 ...
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bioRxiv - Cancer Biology 2022Quote: ... The remaining peptides were loaded onto primed custom-made HILIC SPE micro-columns packed with ZIC-HILIC resin (10 μm particle size, 200 Å pore size, kindly provided by Sequant/Merck, Umea, Sweden) onto supporting C8 disks (Empore ...
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bioRxiv - Immunology 2024Quote: ... The cells were washed three times with 200 µl/well of PBS/Tween and incubated for 10 min at room temperature with 50 µl/well of 3,3‘,5‘,5‘-tetramethylbenzidine (TMB)/H2O2 peroxidase substrate (Merck KGaA, cat. no. T4444). The reaction was stopped by addition of 50 µl/well of 1 M HCl and absorbance read at 450 nm using a GloMax microplate reader (Promega ...
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bioRxiv - Immunology 2024Quote: ... The cells were incubated for 24h at 37°C and 5% CO2 in the presence of 10-9 M of mifepristone (Merck KGaA, Darmstadt, Germany) to allow expression of the G protein ...
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bioRxiv - Synthetic Biology 2023Quote: ... by centrifuging the purified enzyme preparations (4,000×g, 4°C) using AmiconTM Ultra-15 centrifugal filters of 10 kDa-pore size (Merck-MilliPoreSigma, Burlington, MA, USA). The absorbance at 280 nm (A280 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The protein preparation was then concentrated to 300 – 500 μL volume using Amicon Ultra centrifugal filter units with 10 kD cut-off (Merck KGaA, Darmstadt, Germany) and injected into Superdex 75 10/300 GL size exclusion column attached to ÄKTA micro chromatography system (Cytiva ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were dissolved in 50 μL of Milli-Q water and filtered (0.20 μm pore size, 10 mm diameter, Merck Millipore, Burlington, MA, US)71.
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bioRxiv - Molecular Biology 2023Quote: ... The flow through and wash fractions were concentrated to 3-5 mg/mL using Amicon ultrafiltrators (10 kDa MWCO, Merck Millipore, Darmstadt, Germany). All fractions were combined and dialyzed 2x over night at 4 °C against 5 L 10 % (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 mM EDTA) with 1 µl lyticase (17 U/ µl in 10 mM KPO4 pH 7, 50% glycerol, Merck >2000 U/mg L2524), heated to 50°C for 2 min before addition of 40 µl molten CleanCut agarose (Bio-Rad 1703594) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins purified under native conditions were concentrated to 400 μL using a 10 kDa molecular weight cutoff (MWCO) Amicon Ultra-4 centrifugal spin concentrator (Merck-Millipore, Burlington, MA) and purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 column (Cytiva) ...
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bioRxiv - Immunology 2023Quote: ... The resulting Fabs were buffer exchanged into PBS and then concentrated down using Amicon® 10 kDa Ultra-15 Centrifugal Filter Units (Merck Millipore UFC9010). Selected fractions resulting from the size-exclusion chromatography were pooled together and concentrated again for later use.
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bioRxiv - Neuroscience 2023Quote: ... dermis and epidermis were mechanically separated and the dermal part was collected in fibroblast cultivation medium (DMEM/F12 + 100 U/ml penicillin 100 µg/ml streptomycin [pen/strep; both: Thermo Fisher Scientific, Waltham, MA, USA] + 10% fetal calf serum [FCS; Merck, Darmstadt, Germany]).
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bioRxiv - Genetics 2023Quote: ... and neo-resistance MEFs were established from WT Crl:CD1 (ICR) or ICRneo [27] foetuses at 14 d post-coitus and inactivated using 10 μg/mL mitomycin C (#M0503; Merck KGaA, Darmstadt, Germany). MEFs were cultured in the primary medium [Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Neuroscience 2023Quote: ... TSPO-/- MPAs were grown in T25 flasks until confluent before being stored in liquid nitrogen (10% v/v dimethyl sulfoxide (DMSO; catalogue # D2650, Merck Life Sciences (UK)) until TSPO genotypes had been confirmed ...
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bioRxiv - Plant Biology 2023Quote: ... The sections were then incubated for 1 h with 700 µL of a 10% (v/v) DMSO/3% (v/v) IGEPAL® CA-630 (Merck, Darmstadt, Germany) in MTSB ...
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bioRxiv - Biochemistry 2023Quote: Purified NanoLuc was concentrated to a final concentration of ∼10 mg/mL using Centrifugal Filter Units AmiconR Ultra-15 UltracelR-3K (Merck Millipore Ltd., Ireland). Concentrated NanoLuc was mixed with a 4 molar excess of FMZ non-oxidizable analogue azaFMZ (stock solution of azaFMZ was 10 mM in isopropanol) ...
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bioRxiv - Cell Biology 2024Quote: ... Peak fractions were pooled and concentrated using an Amicon Ultra-0.5 centrifugal filter device with a molecular weight cut-off of 30 or 10 kDa (Merck, cat. # UFC503024 or UFC501008). D-desthiobiotin in the peak fraction was diluted more than 100-fold by a repeating dilution with EB and the centrifugal concentration ...
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bioRxiv - Plant Biology 2024Quote: ... or standards (gallic acid in the range of 0–80 µg ml-1) were mixed with 200 µl of 10% Folin-Ciocalteu’s phenol reagent (Sigma-Aldrich, Merck, Burlington MA, USA) and incubated at 25°C in the dark for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked 1h in 10% donkey serum in PBST and incubated overnight with primary antibodies for anti-GFAP (1:250, Merck Life Science, Hpa056030) and anti-MAP2 (1:200 ...
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bioRxiv - Bioengineering 2024Quote: ... We performed gel electrophoresis on a 2% agarose gel with 10 µl 10 mg/mL ethidium bromide (EtBr) submerged in 1.5L 1X TBE with 150 µl 10 mg/mL EtBr (Sigma-Aldrich cat no. E1510; Merck KGaA, Darmstadt, Germany). Wells in the gel were loaded with 10 µl amplified DNA ...
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bioRxiv - Cell Biology 2020Quote: ... tonsil using a ProteoExtract Complete Mammalian Proteome Extraction Kit (cat#539779, Merck Millipore, Darmstadt, Germany), following tissue homogenization with a Precellys 24 (Bertin Instruments ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were counted using the Muse® Count & Viability Kit (Luminex/Merck, Poznan, Poland) according to the manufacturer’s protocol and analyzed using Muse Cell Analyser (Sigma-Aldrich/Merck ...
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bioRxiv - Biochemistry 2022Quote: ... Hemoglobin concentration in the PBS solutions was measured using the Hemoglobin Assay kit (Merck, MAK115).
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bioRxiv - Developmental Biology 2021Quote: PLA was performed using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Merck, USA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... coli NovaBlue competent cells using the Novagen pETBlue-1 Perfectly Blunt Cloning Kit (Merck KGaA). The E ...
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bioRxiv - Cancer Biology 2020Quote: The ChIP assay was carried out using EZ ChIP™ Chromatin Immunoprecipitation kit (Merck-Millipore) according to the manufacture’s instruction ...
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bioRxiv - Microbiology 2020Quote: DNA extraction was carried out using the REDExtract-N-Amp™ tissue kit (Merck, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2022Quote: Apoptotic cell death was determined by In Situ cell death detection kit (Merck, Cat#11684795910) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... Commercial standards of OCFAs (Odd Carbon Straight Chains Kit containing 9 FAs, OC9, Merck, Germany) were converted into their FAMEs using the same method employed with the yeast samples ...
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bioRxiv - Microbiology 2021Quote: ... The consumption of nitrate was monitored using the Spectroquant nitrate test kit HC707906 (Merck, Germany). Sodium nitrate (10 mM ...
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bioRxiv - Neuroscience 2021Quote: ... and then quantified by qPCR using the KAPA Library Quantification Kit (KapaBiosystems, Merck, Darmstadt, Germany) before amplification Illumina’s cBot ...
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bioRxiv - Cancer Biology 2022Quote: RIP was performed according to the Magna-RIPTM RNA-binding protein immunoprecipitation kit (Millipore/Merck) as previously described (Papoutsoglou et al ...
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bioRxiv - Molecular Biology 2022Quote: The concentration of protein extracts was determined by BCA assay kit with BSA standards (Merck).
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bioRxiv - Microbiology 2023Quote: ... The extracted DNA was purified by silica-based gel extraction kit (Merck Biosciences, Bangalore, India) as per the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... PLA was then carried out using the Duolink® In Situ Red starter kit (Merck) according to the manufactureŕs instructions ...
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bioRxiv - Molecular Biology 2024Quote: The strains and samples were extracted using the RED Extract Plant PCR kit from Merck KGaA ...
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bioRxiv - Biochemistry 2024Quote: ... 20 mM imidazole) with protease inhibitor cocktail (complete ultra protease inhibitor kit, Merck, Darmstadt, Germany), 100 U DNAse I ...
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bioRxiv - Molecular Biology 2024Quote: ... The cholesterol efflux assay was performed according to the kit instructions (Sigma-Aldrich MAK192, Merck) without FBS present ...
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bioRxiv - Synthetic Biology 2023Quote: ... RNA was extracted from the microplate using the RNAspin Mini Kit (Merck; GE25-0500-71) and quantified using a NanoDrop™ Spectrophotometer (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: Endothelial tube formation was monitored using an in vitro angiogenesis assay kit (Merck Millipore ECM625) following the manufacturer’s instructions ...