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Citations for Merck :
1751 - 1800 of 2050 citations for Rat Interleukin 10 Receptor Beta IL10Rb ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... We discarded the supernatant and carefully resuspended the pellet in 800 μl M2B-taxol (M2B supplemented with 10 μM taxol (T7402, Merck, Darmstadt, Germany).
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bioRxiv - Biophysics 2023Quote: ... containing 0.3□mM biotin and concentrated at +4 °C to 25 μl volume using Amicon ®Ultra 10 kDa MWCO centrifugal filters (Merck Millipore).
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bioRxiv - Neuroscience 2023Quote: ... Flies were raised at 25℃ and collected on the day of eclosion and kept in the dark for 7-10 days on the fly media containing 0.4 mM all trans-Retinal (Merck, St. Louis, MO). The experiments were performed with the “8-well” chamber (16 mm diameter x 10 mm height ...
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bioRxiv - Biochemistry 2023Quote: PrPC was filtered through a 100 kD membrane filter in 10 mM Bis Tris pH 6.5 (Amicon ULTRA 0.5 mL 100K 96PK, UFC510096, Merck Life Science UK Ltd) to remove aggregates and then diluted into reaction buffer (50 mM Na-phosphate ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation on ice for 10 min and loaded on SDS-PAGE gel and wet transfer onto PVDF membrane (Merck, Cat. # IPFL00010). Thereafter ...
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bioRxiv - Synthetic Biology 2024Quote: ... Protein containing fractions were pooled and buffer exchanged using SnakeSkin dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096), as described previously (44).
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bioRxiv - Molecular Biology 2023Quote: ... 10% glycerol) supplemented with 1 mg/ml lysozyme and one tablet of SIGMAFAST Protease Inhibitor Cocktail Tablets EDTA-Free (Merck KGaA, Germany), and disrupted by sonication (10 cycles of 30 sec at 40 % intensity in ice ...
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bioRxiv - Molecular Biology 2023Quote: ... filtered through a 0.45 µm filter and supplemented with 10 mM HEPES buffer (BI, 03-025-1B) and 12.5 µg/ml polybrene (Merck, TR-1003-G). MLV-based GFP-reporter (pNCA-GFP ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 5 μg of plasmid and dsRNA were transfected into BmN4 cells (8 × 105 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche) every 3 days four times ...
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bioRxiv - Cell Biology 2024Quote: ... hTERT-RPE1-Flp-In-Parental cells were co-transfected with a 10:1 ratio of pOG44 and either pCDNA5 FRT/TO-NeoR-HA-VPS35-WT or -[D620N] using Genejuice (Merck Millipore; 70967), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... a concentrated lipid mixture stock solution of fatty acids conjugated to fatty acid-free BSA (10% solution in PBS) (Merck cat# A1595) was created as previously described71 ...
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bioRxiv - Microbiology 2024Quote: ... 20 °C) from NO2- replete active cultures using Amicon Ultra-15 10 kDa cut-off centrifugal filter units (Merck Millipore, Darmstadt, Germany). Concentrated cells were washed with and resuspended in substrate-free medium (pH 6 ...
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bioRxiv - Cell Biology 2024Quote: ... Base elastomer was thoroughly mixed with curing agent at a ratio of a ratio of 10:1, before degassing in a vacuum desiccator (Scienceware, 999320237) with a KNF Laboport N96 diaphragm vacuum pump (Merck, Z675091-1EA). A Si wafer with positive features was placed in a disposable aluminium dish (VWR ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 4.5–6 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). The second transfection was performed 3 days after the first transfection and the cells were harvested after an additional 5 days ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 3–5 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). Transfection was repeated 2 days and 5 days after the first transfection and the cells were harvested after an additional 4 days ...
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bioRxiv - Microbiology 2024Quote: ... the fractions containing each component of the assembled PhoP-TAC were concentrated using Amicon Ultra centrifugal filters (10 kDa MWCO, Merck Millipore, Inc.). In addition ...
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bioRxiv - Cancer Biology 2024Quote: ... 09499-14) were subjected to SDS-PAGE on a 10% polyacrylamide gel and subsequently transferred to Immobilon-P PVDF-membrane (Merck Millipore, IPVH85R). The membrane was blocked with 5% (w/v ...
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bioRxiv - Cancer Biology 2021Quote: ... and cell count and viability were determined with the Muse Count & Viability Kit (Merck) on the Muse Cell Analyser (Merck) ...
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bioRxiv - Cell Biology 2020Quote: ... and nucleoproteins were extracted using a nuclear protein extraction kit (Merck Millipore, Darmstadt, Germany). Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS ...
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bioRxiv - Cell Biology 2022Quote: The cell cycle was determined using Muse Cell Cycle Kit (Luminex/Merck, Poznan, Poland) using the manufacturer’s protocol ...
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bioRxiv - Physiology 2020Quote: ... Total proteins from the supernatant were quantified using a Bradford Protein Assay Kit (Merck) and subjected to electrophoresis on 15% SDS–PAGE gels ...
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bioRxiv - Biochemistry 2022Quote: ... and dialyzed against PBS using a Pur-A-Lyzer™ Midi Dialysis Kit (Merck).
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Induction of autotetraploidy and microbiome associations mediate differential responses to pathogensbioRxiv - Plant Biology 2021Quote: ... RNA was extracted using the Spectrum Plant Total RNA Kit (Merck / MilliporeSigma, MO, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2019Quote: ... and ligated in a three-way ligation reaction using a Rapid Ligation Kit (Merck) for 30 minutes at room temperature.
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bioRxiv - Plant Biology 2021Quote: ... we used a commercial OxyBlot™ kit (cat n. S7150, Merck KGaA, Darmstadt, Germany). Sample preparation ...
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bioRxiv - Bioengineering 2020Quote: ... or ApopTag® ISOL fluorescence apoptosis detection kit (Merck Millipore, Merck KGaA, Darmstadt, Germany) according to the manufacturer’s protocol.
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bioRxiv - Physiology 2020Quote: ... The subsequential RNA immunoprecipitation (RIP) was performed using the Magna RIP kit (Merck Millipore) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... performed by the GeneElute Mammalian Total RNA purification kit (Sigma Aldrich-Merck KGaA Germany). The RNA was treated with deoxyribonuclease (DNAse ...
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The Vagus Nerve Mediates the Physiological but not Pharmacological Effects of PYY3-36 on Food IntakebioRxiv - Physiology 2020Quote: Mice were genotyped using the KAPA2G Fast HotStart Mouse Genotyping Kits (Merck, Southampton, UK) in a PCR ...
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bioRxiv - Cancer Biology 2022Quote: ... ATP:ADP ratio assays were performed using an ADP/ATP ratio assay kit (Merck, MAK135).
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bioRxiv - Physiology 2022Quote: ... Protein carbonylation was assessed with an Oxyblot protein oxidation detection kit (#S7150, Merck Millipore) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... A Magna MeRIP™ m1A Kit (Merck Millipore, MA, USA; Cat. No. 17–10499) was used to perform immunoprecipitation (IP ...
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bioRxiv - Pathology 2024Quote: ... was used to detect the signals from the membranes with Immobilon kit (Merck, WBKLS0500).
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bioRxiv - Microbiology 2023Quote: ... DNA and RNA was amplified using the Complete Whole Transcriptome Amplification kit (WTA2, Merck), and resulting PCR products were further purified and prepared for sequencing with the Nextera XT kit (Illumina) ...
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bioRxiv - Molecular Biology 2022Quote: PLA was performed on Vero cells using the Duolink® PLA kit (Merck, DUO92014) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were lysed with 100 μl with RIP lysis buffer (Magna RIP kit, Merck) supplemented with 10 U RNaseIn and 0.5 μl protease-inhibitor cocktail ...
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bioRxiv - Neuroscience 2023Quote: ... and flat-embedded in Epoxy resin (Epoxy embedding medium kit, Merck KGaA, Darmstadt, Germany; #). UC7 LEICA ultramicrotome (UC7 ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted using the GENELUTE™ mammalian genomic DNA Miniprep kit (Merck) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: Drug efflux test was performed using Multidrug Resistance Direct Dye Efflux Assay kit (Merck), according to manufacturer’s instruction ...
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bioRxiv - Biochemistry 2024Quote: ... cells were collected in MLB buffer (RAS Activation Assay Kit, Merck Millipore, 17-218) after 30 min of 10 ng/mL EGF (Preprotech ...
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bioRxiv - Microbiology 2024Quote: ... Methionine synthesis was measured using the Methionine Assay Kit (Sigma-Aldrich, Merck, Darmstadt, Germany). For measurements ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant NS1 was purified using a Ni2+-immobilized column (His-Bind Purification Kit; Merck) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... Glycolipid extracts of up to 15 μl were applied evenly onto half TLC silica plates (20 x 10 cm, Merck KGaA, Darmstadt, Germany). After drying ...
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bioRxiv - Microbiology 2021Quote: ... the cell layers were washed again with PBS and then lysed for 10 min with PBS containing 1% Elugent (Merck Millipore, Darmstadt, Germany) and 0,0625% Antifoam B (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... Chlorophyll a content was determined using a microplate reader (BMG Polarstar Optima) after filtration of the sub-samples (5 to 10 mL) through Whatman GF/F glass circles (25 mm) (Merck KGaA, Darmstadt, Germany) and 90% acetone extraction of the filtrate for 24 h in the dark at 4 °C [74].
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bioRxiv - Biophysics 2019Quote: ... ES products from all three days were thawed at 4 °C and pooled to be concentrated 720 times with Amicon® Ultra-15 Centrifugal Filter Unit 10 kDa cut-off (Merck, cat#UFC901024). The concentrate was used for EV separation.
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bioRxiv - Bioengineering 2019Quote: ... was diluted with ultrapure water (30 mL LUDOX® TM50 to 400 mL water) followed by addition of ammonia (10 mL) (NH3, 28-30%, Merck, Darmstadt, Germany) and (3-aminopropyl)trimethoxysilane (24 mL ...
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bioRxiv - Biochemistry 2020Quote: ... The imidazole of the elution fraction was removed until the concentration of imidazole was below 1 mM using Amicon® centrifugal filters (0.5 ml size, GlnA1: 30 kDa and GlnK1: 10 kDa) (Merck KGaA, Darmstadt, Germany). His6-sP26 was purified as described above with the following additional steps ...
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bioRxiv - Cell Biology 2021Quote: ... 1.5E05 cells were seeded onto a T25 Matrigel coated flask in StemFlex medium supplemented with 10 µM rock inhibitor (Merck Millipore, Y-27632). Medium was refreshed the following day without rock inhibitor and lived cells were sent over to Cell Line Genetics when 20-30% confluence was reached ...
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bioRxiv - Cell Biology 2021Quote: ... The fractions containing the target proteins were pooled and concentrated to less than 0.5 ml using an Amicon Ultra concentrator (Merck Millipore; 10 kDa MWCO). The concentrate was applied to a Superdex 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare ...