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Citations for Merck :
1451 - 1500 of 2650 citations for Rat Insulin Like Growth Factor Binding Protein 5 IGFBP5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... reduced with 5 mM TCEP for 30 min and alkylated with 10 mM iodoacetamide (Merck, I1149) for 30 min ...
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bioRxiv - Cell Biology 2023Quote: Oocytes were cultured in G-IVF PLUS (Vitrolife, #10136) under mineral oil (Merck, # 8042-47-5) at 37°C in 5% O2 ...
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bioRxiv - Cell Biology 2022Quote: ... a SeQuant ZIC-pHILIC (100 mm, 2.1 mm I.D. and 5 μm particle size, Merck, Germany) column was used ...
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bioRxiv - Cancer Biology 2022Quote: ... For final and irreversible sample dehydration a graded serie (25%, 50%, 75% and 100%, 5 min each) of 1,1,1,3,3,3-hexamethyldizilazane (HDMS, Merck, Millipore) was used ...
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bioRxiv - Cell Biology 2023Quote: ... Parasitemia was maintained below 5% and determined via Hemacolor staining of blood smears (Sigma-Aldrich, Merck) using a Nikon Eclipse E100 microscope (Nikon Corporation ...
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bioRxiv - Biophysics 2023Quote: ... 6809-1102) PBS (3 × 50 μL) before incubation with gold nanoparticles (5 μL, 0.1 μm, Merck) for 20 minutes.
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bioRxiv - Cell Biology 2023Quote: ... Preparations were thereafter fixed with 100% methanol for 15 s and stained with 5% Giemsa (Merck) for 15 minutes ...
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bioRxiv - Immunology 2023Quote: Resuspended HLA-I-eluted samples were centrifuged through 5 kDa cutoff filters (Merck Millipore #UFC3LCCNB-HMT) and vacuum-dried ...
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bioRxiv - Neuroscience 2023Quote: ... 5 % Natural Goat Serum (Goat serum from controlled donor herd, G6767-100ML, Sigma Aldrich, Merck, Germany) and primary antibodies (see Table 3 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... For removal of chorion embryos were incubated for 5 mins with 1 mg/ml Pronase (Merck) then washed with culture medium and flash-frozen in liquid nitrogen ...
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bioRxiv - Genetics 2024Quote: ... Membranes were blocked for a minimum of 30 min in 5% Skim Milk Powder (Merck, #70166) in PBS + 0.1% Tween-20 (PBS-T ...
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bioRxiv - Immunology 2024Quote: ... slides were rinsed in double-distilled water and counterstained with Hoechst dye (5 μg/mL; Merck). Stained slides were subsequently imaged using an LSM700 confocal microscope (Zeiss).
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bioRxiv - Molecular Biology 2024Quote: ... animals were transferred to 10 cm NGM agar plates containing 5-FU (10 µM) (Merck, #F6627) to prevent progeny production ...
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bioRxiv - Molecular Biology 2024Quote: ... animals were transferred to 10 cm NGM agar plates containing 5-FU (10 µM) (Merck, #F6627) to prevent progeny production ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were permeabilized and blocked with PBS containing 0.1% Triton X (#9005-64-5, Merck, Germany), 5% ...
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bioRxiv - Biochemistry 2024Quote: ... the spacer region between them and flanking regions of 5 bp were ordered from Merck (Germany). The 5’ end of sense strand of each oligonucleotide was labeled with Cyanine 5 dye (Cy5 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The pure proteins were concentrated using a 3000-high molecular weight cutoff centrifugal filter (Merck-Millipore). The concentrated proteins were then subjected to size-exclusion chromatography using a Superdex-75 GL column (GE healthcare pre-equilibrated with 25 mM Tris-HCl 6.8 ...
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bioRxiv - Molecular Biology 2021Quote: ... Total protein stain (30% methanol, 6.7% acetic acid, 0.0005% Fast Green FCF (Merck KGaA, Darmstadt, Germany)) stain was used as a loading control and imaged prior to blocking ...
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bioRxiv - Biophysics 2019Quote: ... Protein was transferred overnight at 10 V to an Immobilon-P membrane (Merck Millipore; Burlington, MA) in 25 mM Tris ...
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bioRxiv - Cell Biology 2019Quote: ... Supernatant was collected and protein concentration was determined using the Direct Detect® Spectrometer (Merck Millipore). The proteins (30 µg ...
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bioRxiv - Cancer Biology 2019Quote: The uncharged polystyrene 96-well plate were coated with different recombinant proteins-Collagen IV (Merck Millipore), P-Selectin ...
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bioRxiv - Bioengineering 2019Quote: ... proteins were separated by SDS-PAGE and transferred onto PVDF (polyvinylidene fluoride) membranes (Merck Millipore, DE). The membranes were then blocked for 1 h in 5% non-fat milk in PBST and incubated with CD64 and CD16 antibodies and β-actin antibody respectively overnight at 4°C ...
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bioRxiv - Genomics 2021Quote: ... Prior to protein quantification milk was diluted 1:2 in assay buffer (Merck-Millipore, Burlington, USA). Concentrations for 15 cytokines (IL-1α ...
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bioRxiv - Biochemistry 2020Quote: ... Flag-tagged proteins were eluted by incubation with 150 μg/mL Flag tag peptide (Merck; F3290) with 0.1% digitonin in solubilisation buffer for 30 min at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Protein samples were transferred and western blotted using anti phospho-T187 p27KIP1 antibody (Merck, 06-996). Band intensity was revealed using ECL reagent (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... downward capillary action was used to transfer protein to immune-blot PVDF membrane (Merck cat# IPVH00010). Membranes were blocked with 5% milk in wash buffer (0.05% Tween in PBS ...
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CAP1 and cofilin1 cooperate in neuronal actin dynamics, growth cone function and neuron connectivitybioRxiv - Neuroscience 2020Quote: ... Equal protein amounts were separated by SDS PAGE and blotted onto a polyvinylidene difluoride membrane (Merck) by using a Wet/Tank Blotting System (Biorad) ...
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bioRxiv - Immunology 2022Quote: ... Protein was digested by incubating membrane at 50°C for 1 h with proteinase K (Merck) diluted in PK-SDS buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein-containing fractions were dialyzed in a cellulose membrane tube (14 kDa MWCO, Merck-Millipore), first ...
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bioRxiv - Microbiology 2022Quote: Wild type and mutant CANC proteins were expressed in Escherichia coli Rosetta (DE3) cells (Merck Millipore) as previously described (Betancor et al. ...
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bioRxiv - Neuroscience 2021Quote: ... 500–1000 µg of total protein (estimated by Direct Detect® Infrared Spectrophotometer assay, Merck Millipore), were incubated overnight with 2 µg of antibody raised against an intracellular epitope in mouse NLGN1 (aminoacids 826 to 843 ...
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bioRxiv - Neuroscience 2021Quote: ... Purified protein was concentrated to 8 mg/mL using 30 kDa cut-off AMICON concentrators (Merck).
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Proteins were separated by SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Immobilon-P, Merck Millipore). Proteins of interest were detected using the following primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to a PVDF membrane (0.45µm Immobilon®-P, Merck Millipore Ltd., VIC, Australia) at 100 V in chilled transfer buffer containing 25 mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse monoclonal IgG2A antibody to myelin Proteolipid Protein (clone PLPC1, MAB388, MERCK-Millipore, 1:100). All slides were mounted using Mowiol mounting medium (475904 ...
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bioRxiv - Immunology 2022Quote: ... Proteins were resolved on SDS–PAGE gels and transferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore). The PVDF membranes were then blocked with 5% skim milk and sequentially incubated with primary and secondary antibodies ...
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bioRxiv - Biochemistry 2019Quote: All proteins were expressed in E.coli Rosetta-2 DE3 cells (EMD Millipore, Merck KGaA, Darmstadt, Germany). Cells were transformed via heat shock (42°C for 35 secs ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was concentrated to ~65 mM using a MWCO 30,000 Amicon Ultra Centrifugal Filter (Merck), flash-frozen and stored at −80 °C until use.
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bioRxiv - Microbiology 2020Quote: Viral proteins were separated by SDS-PAGE and transferred to polyvinylidene disulfide (PVDF) membranes (Merck Millipore). After blocking the membranes with 5% skim milk ...
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bioRxiv - Plant Biology 2019Quote: ... Cells were pelleted by centrifugation and lysed in Bugbuster™ protein extraction reagent (Merck; Darmstadt, Germany) by continuous agitation for 20 min at room temperature ...
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bioRxiv - Biochemistry 2020Quote: Purified proteins were concentrated using Amicon® Ultra-4 Ultracel®-10K (MWCO: 10.000 Da; Merck). Protein concentrations were quantified using the calculated molar extinction coefficient ε280 (29).
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bioRxiv - Cell Biology 2021Quote: ... Purified proteins were were concentrated > 60 μM using Amicon Ultra centrifugal filters (Merck; MWCO 3 kDa), rebuffered to 50 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... The collected protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore, Beijing China) with 100 kDa cut off and frozen at −80 °C.
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bioRxiv - Microbiology 2021Quote: ... Protein samples for mass spectrometry were purified and concentrated using ZipTipC-18 (Merck, Millipore, Darmstadt, Germany).
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bioRxiv - Microbiology 2021Quote: ... Protein signals on the membranes were detected with Immobilon Forte Western HRP substrate (Merck, Darmstadt, Germany) and the ChemiImager Ready (Alpha Innotec,San Leandro USA).
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bioRxiv - Biochemistry 2021Quote: ... Proteins were eluted with 50 μl of 100 μg/mL of 3X FLAG tag peptide (Merck) in solubilization buffer followed by acetone precipitation (overnight ...
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bioRxiv - Biochemistry 2021Quote: ... 500 μg protein was added to spin columns (Pierce) containing anti-FLAG M2 affinity resin (Merck) and allowed to incubate rotating for two hours at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were concentrated using a centrifugal filter unit (3 kDa MWCO, Merck Millipore; Cat. No. UFC900324) at 3500 rpm and 4°C ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... proteins were transferred to a 0.45 μm PVDF membrane (#IPVH85R; Sigma-Aldrich/Merck, Zwijndrecht, The Netherlands) using an electroblotting device (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... and the protein was concentrated in a centrifugal concentrator (Amicon MWCO 3 kDa: Merck, Darmstadt, Germany) and stored at −20 °C in dialysis buffer (150 mM NaCl ...