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Citations for Merck :
101 - 150 of 1852 citations for Recombinant Mouse EGFL6 Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
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bioRxiv - Biophysics 2023Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosetta 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 µg/ml kanamycin and 34 µg/ml chloramphenicol ...
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bioRxiv - Biophysics 2022Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
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bioRxiv - Molecular Biology 2024Quote: ... or HIS-Select Nickel Affinity Gel in a gravity-flow column (Merck, Darmstadt, Germany), followed by washing with binding buffer with and without 1.3% Triton X-100 ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Neuroscience 2021Quote: ... Detection of newly synthesized proteins was carried out by an anti-puromycin antibody (clone 12D10, mouse-monoclonal, MABE343; Merck), an anti-HuD antibody (ab96474 ...
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bioRxiv - Microbiology 2022Quote: ... HRP-conjugated anti-goat or anti-mouse IgG (dilution 1:1,000) were added and protein complexes were visualized using o-phenylenediamine (Merck). The absorbance was then measured at 490 nm employing PowerWave HT (Bio-Tek Instruments ...
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bioRxiv - Microbiology 2022Quote: ... The sections were then incubated overnight in PBS with 0.2% BSA and 0.05% Tween-20 with primary antibodies directed against SARS-CoV-2 Nucleocapsid Protein (1:500; mouse monoclonal, # ZMS1075, Merck); Iba1 (1 ...
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bioRxiv - Cell Biology 2020Quote: ... the GFP-Trap bead bound fraction was incubated with recombinant TEV (Merck) overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... then 1,000 IU recombinant human chorionic gonadotropin alpha (rhCG, OVIDREL, Merck Serono) was injected on day 9 ...
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bioRxiv - Microbiology 2021Quote: 10His-tagged MA gene was expressed in Escherichia coli (E. coli) strain BL21 (DE3) (Merck, Darmstadt, Germany). The 10His-tagged MA protein was initially purified with nickel-NTA affinity column chromatography followed by TEV cleavage ...
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bioRxiv - Plant Biology 2024Quote: ... Phosphorylated SR proteins were detected using a mouse monoclonal α-pan-phosphoepitope SR-specific antibody (1H4, Merck, Catalogue-No.: MABE50) at 0.025 µg/mL ...
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bioRxiv - Biochemistry 2022Quote: ... mouse anti-mouse FAP (Merck, MABC1145), mouse anti-CAR (Millipore ...
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bioRxiv - Genetics 2021Quote: Before the ovaries were stimulated with recombinant FSH (Gonal-F, Merck Serono, Switzerland), downregulation was performed using a GnRH agonist (Decapeptyl ...
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bioRxiv - Zoology 2020Quote: ... Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck) in TE buffer (10 mM Tris-HCI ...
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bioRxiv - Bioengineering 2021Quote: ... recombinant human platelet derived growth factor (hPDGF-AB, 20 ng/ml, Merck, GF106), recombinant human oncostatin M (hOSM ...
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bioRxiv - Cell Biology 2022Quote: All experiments were performed using the BDNF mature recombinant (GF029; Merck Millipore, USA) or the C-ter truncated form of BDNF (BDNFΔC-ter) ...
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bioRxiv - Neuroscience 2023Quote: ... and human recombinant FGF-2 (10 ng/ml; Merck Millipore, Burlington, MA, USA) as mitogens ...
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bioRxiv - Immunology 2023Quote: ... Mo were primed with 200 U/mL human recombinant IFNα2β (Intron A; Merck) for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D ...
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bioRxiv - Plant Biology 2022Quote: ... The filtered supernatant was loaded onto a column packed with cOmpleteTM His-tag purification resin (Merck). His6-proteins were eluted with 4x 2 bead volumes of elution buffer (20 mM Na HEPES ...
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bioRxiv - Evolutionary Biology 2020Quote: ... F2 FLAG-tagged TBP::NveRLR::mCherry and wild-type zygotes were treated with 3% L-Cysteine (Merck Millipore), washed and snap frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Cells were permeabilized with 100% ice-cold methanol for 20 min at -20°C and rehydrated by washing with PBS and then stained for viral hexon protein expression with an anti-hexon primary antibody (mouse; Merck; #MAB8052) and an anti-mouse secondary antibody coupled to AF488 or AF647 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Purified proteins and native protein standard (Supelco-Merck) were loaded in 4-15% gradient mini protean precast native gel (Bio-Rad ...
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bioRxiv - Biophysics 2020Quote: ... the purified mEGFP-tagged NbALFA was concentrated to 1 mg/ml using an Amicon Ultra 30K filter (Merck Millipore) and stored at -80 °C before use ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... plasmid coding for 6x-His-Cas9 were transformed in Rosetta DE3 Novagen competent cells (Merck Millipore, USA). Protein production was induced using autoinduction medium (Formedium ...
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bioRxiv - Genetics 2023Quote: ... imidazole was removed from the eluted His-SIRT5 using ultra-0.5 centrifugal filter units (UFC501096, Merck Millipore). 500 µl of the recombinant SIRT5 elution fractionation was added to the ultra-0.5 centrifugal filter units and centrifuged at 14,000 x g for 15 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was then incubated with 2 mL pre-washed HIS-Select Cobalt Affinity Gel (Merck, UK) with rotation for an hour ...
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bioRxiv - Molecular Biology 2019Quote: ... protocol with the administration of recombinant FSH (Gonal-F, Merck Serono, or Puregon, Merck Sharp & Dohme Corp. ...
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bioRxiv - Cancer Biology 2023Quote: Lysates from HEK293 cells overexpressing FLAG-tagged wild typeTP53INP2 or the TP53INP2-3A were affinity-purified with anti-FLAG-M2 affinity beads (Merck). Then ...
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bioRxiv - Molecular Biology 2022Quote: CobB driven deacetylation of His-PrsAc was analyzed by Western blot with anti-acetyl lysine antibodies (Sigma/Merck) and mass spectrometry ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein G Plus/Protein A Agarose beads (Merck Millipore #IP05) were added to the mix followed by incubation at room temperature for 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA tagged with 6xHis at the C-terminus was introduced into a pNG2 vector (a derivative of pET-3a, Merck-Novagen) linearized with NdeI and BamHI ...
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bioRxiv - Cell Biology 2020Quote: ... After 24 hrs the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore). The HeLa cells stably expressing mouse Cx26 were originally obtained from Dr K ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore).
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... rh-HI was prepared in either 20% acetic acid (v/v from acetic acid glacial, Merck KGaA, Darmstadt, Germany), 0.3 M NaCl (Merck KGaA ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation (10,000 x g, 30 min, RT) and incubated for 1 hour with Ni-NTA His bind resin (Merck) rolling at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Biophysics 2022Quote: ... Both recombinant and synthetic lyophilized peptides were dissolved in 6M guanidine thiocyanate (Merck KGaA, Darmstadt, Germany) and incubated overnight at 1250 rpm ...
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bioRxiv - Genetics 2020Quote: ... which was performed by intramuscular injection with rhFSH (recombinant human follitropin alpha, GONAL-F, Merck Serono) for 8 days ...
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bioRxiv - Neuroscience 2024Quote: The inhibitory activity of novel compounds against human recombinant AChE (hAChE, E.C. 3.1.1.7, purchased from Merck) and human plasmatic butyrylcholinesterase (hBChE ...
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bioRxiv - Cell Biology 2019Quote: ... GAPDH (mouse; Merck), FLAG (mouse ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG (mouse; Merck), FLAG (rat ...
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bioRxiv - Neuroscience 2022Quote: ... FoxP2 (mouse, Merck Millipore ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells transfected with Flag-tagged NSP1 or vector plasmid were treated with the following compounds for 6 h: 10 μM of MG132 (Merck, Cat# 474787), 50 μM of chloroquine (CQ ...
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bioRxiv - Biochemistry 2023Quote: Total cell lysates of HEK293 cells stably expressing C-terminally Flag M2-tagged SIRT4 were incubated overnight at 4°C with anti-Flag M2 agarose beads (Merck, Darmstadt, Germany). The beads were washed three times with washing buffer (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... and applied directly onto a column of 2 ml pre-equilibrated Ni-NTA beads (cOmplete His-Tag Purification Resin from Merck). After several washes with high salt (500 mM NaCl) ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was loaded on to a gravity flow-based Ni-NTA metal affinity column (2 ml beads, cOmplete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... WT—or its mutants fused with a His-tag on the amino-terminus—were expressed in Escherichia coli BL21 (DE3) using pET15b vector (Merck). A single colony was inoculated in 2 mL Luria-Bertani broth (LB ...
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bioRxiv - Microbiology 2021Quote: ... The sonicated cellular extract was spun down and the supernatant has been incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...